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Microbiology Research
Volume 2
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10.4081/mr.2011.e4
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Article

Characterization of AcMNPV with a deletion of ac69 gene

by
Jianhao Ke
,
Jinwen Wang
,
Riqiang Deng
,
Lin Lin
,
Bei Jinlong
,
Liao Yaoping
and
Xunzhang Wang
*
*
Author to whom correspondence should be addressed.
Microbiol. Res. 2011, 2(1), e4; https://doi.org/10.4081/mr.2011.e4
Submission received: 20 June 2011 / Revised: 20 June 2011 / Accepted: 20 June 2011 / Published: 19 July 2011

Abstract

ORF69 (Ac69) of Autographa californica multiple nucleopolyhedrovirus (AcMNPV) is conserved in some baculovirus genomes. Although it has been shown that Ac69 has cap 0-dependent methyltransferase activity and is not required for budded virus production in Spodoptera frugiperda Sf-9 cells, its role in occlusion-derived virus synthesis and virus oral infectivity is not known. This paper describes generation of an ac69 knockout AcMNPV bacmid mutant and analyses of the influence of ac69 deletion on the viral infectivity in Sf-9 cells and Trichoplusia ni larvae so as to investigate the role of ac69 in the viral life cycle. Results indicated that ac69 deletion has little effect on the production rates and morphogenesis of budded virus and occlusion-derived virus in Sf-9 cells. In addition, animal experiment revealed that the deletion mutant did not affect AcMNPV infectivity for Trichoplusia ni larvae in LD50 and LT50 bioassay when administered orally. These results suggest that ac69 may be dispensable for viral infectivity both in vitro and in vivo.

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MDPI and ACS Style

Ke, J.; Wang, J.; Deng, R.; Lin, L.; Jinlong, B.; Yaoping, L.; Wang, X. Characterization of AcMNPV with a deletion of ac69 gene. Microbiol. Res. 2011, 2, e4. https://doi.org/10.4081/mr.2011.e4

AMA Style

Ke J, Wang J, Deng R, Lin L, Jinlong B, Yaoping L, Wang X. Characterization of AcMNPV with a deletion of ac69 gene. Microbiology Research. 2011; 2(1):e4. https://doi.org/10.4081/mr.2011.e4

Chicago/Turabian Style

Ke, Jianhao, Jinwen Wang, Riqiang Deng, Lin Lin, Bei Jinlong, Liao Yaoping, and Xunzhang Wang. 2011. "Characterization of AcMNPV with a deletion of ac69 gene" Microbiology Research 2, no. 1: e4. https://doi.org/10.4081/mr.2011.e4

APA Style

Ke, J., Wang, J., Deng, R., Lin, L., Jinlong, B., Yaoping, L., & Wang, X. (2011). Characterization of AcMNPV with a deletion of ac69 gene. Microbiology Research, 2(1), e4. https://doi.org/10.4081/mr.2011.e4

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