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Article

Novel Green Biosynthesis of 5-Fluorouracil Chromium Nanoparticles Using Harpullia pendula Extract for Treatment of Colorectal Cancer

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Department of Pharmaceutics and Clinical Pharmacy, Faculty of Pharmacy, Sohag University, P.O. Box 82524, Sohag 82524, Egypt
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Department of Pharmacognosy, Faculty of Pharmacy, Sohag University, Sohag 82524, Egypt
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Department of Medical Microbiology and Immunology, Faculty of Medicine, Assiut University, Assiut 71516, Egypt
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Department of Industrial Pharmacy, Faculty of Pharmacy, Assiut University, Assiut 71516, Egypt
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Chemistry Department, College of Science, Taibah University, Madinah 42353, Saudi Arabia
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Chemistry Department, Faculty of Science, Sohag University, Sohag 82524, Egypt
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Department of Chemistry, Faculty of Science, New Valley University, Al-Kharja 72511, Egypt
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Department of Biochemistry, Faculty of Pharmacy, Nahda University (NUB), Beni-Sueif 62511, Egypt
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Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Nahda University (NUB), Beni-Suef 62511, Egypt
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Author to whom correspondence should be addressed.
Academic Editor: Crispin R. Dass
Pharmaceutics 2021, 13(2), 226; https://doi.org/10.3390/pharmaceutics13020226
Received: 5 December 2020 / Revised: 28 January 2021 / Accepted: 2 February 2021 / Published: 6 February 2021
Colorectal cancer (CRC) is the third highest major cause of morbidity and mortality worldwide. Hence, many strategies and approaches have been widely developed for cancer treatment. This work prepared and evaluated the antitumor activity of 5-Fluorouracil (5-Fu) loaded chromium nanoparticles (5-FuCrNPs). The green biosynthesis approach using Harpullia (H) pendula aqueous extract was used for CrNPs preparation, which was further loaded with 5-Fu. The prepared NPs were characterized for morphology using scanning and transmission electron microscopes (SEM and TEM). The results revealed the formation of uniform, mono-dispersive, and highly stable CrNPs with a mean size of 23 nm. Encapsulation of 5-Fu over CrNPs, with a higher drug loading efficiency, was successful with a mean size of 29 nm being produced. In addition, Fourier transform infrared (FTIR) and X-ray diffraction pattern (XRD) were also used for the investigation. The drug 5-Fu was adsorbed on the surface of biosynthesized CrNPs in order to overcome its clinical resistance and increase its activity against CRC cells. Box–Behnken Design (BBD) and response surface methodology (RSM) were used to characterize and optimize the formulation factors (5-Fu concentration, CrNP weight, and temperature). Furthermore, the antitumor activity of the prepared 5-FuCrNPs was tested against CRC cells (CACO-2). This in vitro antitumor study demonstrated that 5-Fu-loaded CrNPs markedly decreased the IC50 of 5-Fu and exerted more cytotoxicity at nearly all concentrations than 5-Fu alone. In conclusion, 5-FuCrNPs is a promising drug delivery system for the effective treatment of CRC. View Full-Text
Keywords: chromium nanoparticles; 5-Flourouracil; colorectal cancer; green biosynthesis chromium nanoparticles; 5-Flourouracil; colorectal cancer; green biosynthesis
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MDPI and ACS Style

Saddik, M.S.; Elsayed, M.M.A.; Abdelkader, M.S.A.; El-Mokhtar, M.A.; Abdel-Aleem, J.A.; Abu-Dief, A.M.; Al-Hakkani, M.F.; Farghaly, H.S.; Abou-Taleb, H.A. Novel Green Biosynthesis of 5-Fluorouracil Chromium Nanoparticles Using Harpullia pendula Extract for Treatment of Colorectal Cancer. Pharmaceutics 2021, 13, 226. https://doi.org/10.3390/pharmaceutics13020226

AMA Style

Saddik MS, Elsayed MMA, Abdelkader MSA, El-Mokhtar MA, Abdel-Aleem JA, Abu-Dief AM, Al-Hakkani MF, Farghaly HS, Abou-Taleb HA. Novel Green Biosynthesis of 5-Fluorouracil Chromium Nanoparticles Using Harpullia pendula Extract for Treatment of Colorectal Cancer. Pharmaceutics. 2021; 13(2):226. https://doi.org/10.3390/pharmaceutics13020226

Chicago/Turabian Style

Saddik, Mohammed S., Mahmoud M.A. Elsayed, Mohamed S.A. Abdelkader, Mohamed A. El-Mokhtar, Jelan A. Abdel-Aleem, Ahmed M. Abu-Dief, Mostafa F. Al-Hakkani, Hatem S. Farghaly, and Heba A. Abou-Taleb 2021. "Novel Green Biosynthesis of 5-Fluorouracil Chromium Nanoparticles Using Harpullia pendula Extract for Treatment of Colorectal Cancer" Pharmaceutics 13, no. 2: 226. https://doi.org/10.3390/pharmaceutics13020226

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