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Viruses 2016, 8(2), 47;

NF45 and NF90 Bind HIV-1 RNA and Modulate HIV Gene Expression

Department of Medical Microbiology and Immunology, Creighton University, Omaha, NE 68178, USA
The Nebraska Center for Virology, University of Nebraska, Lincoln, NE 68583, USA
Author to whom correspondence should be addressed.
Received: 6 November 2015 / Revised: 27 January 2016 / Accepted: 4 February 2016 / Published: 16 February 2016
(This article belongs to the Section Animal Viruses)
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A previous proteomic screen in our laboratory identified nuclear factor 45 (NF45) and nuclear factor 90 (NF90) as potential cellular factors involved in human immunodeficiency virus type 1 (HIV-1) replication. Both are RNA binding proteins that regulate gene expression; and NF90 has been shown to regulate the expression of cyclin T1 which is required for Tat-dependent trans-activation of viral gene expression. In this study the roles of NF45 and NF90 in HIV replication were investigated through overexpression studies. Ectopic expression of either factor potentiated HIV infection, gene expression, and virus production. Deletion of the RNA binding domains of NF45 and NF90 diminished the enhancement of HIV infection and gene expression. Both proteins were found to interact with the HIV RNA. RNA decay assays demonstrated that NF90, but not NF45, increased the half-life of the HIV RNA. Overall, these studies indicate that both NF45 and NF90 potentiate HIV infection through their RNA binding domains. View Full-Text
Keywords: HIV; NF45; NF90; transcription; RNA regulation HIV; NF45; NF90; transcription; RNA regulation

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Li, Y.; Belshan, M. NF45 and NF90 Bind HIV-1 RNA and Modulate HIV Gene Expression. Viruses 2016, 8, 47.

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