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Expression Analysis of the NAC Transcription Factor Family of Populus in Response to Salt Stress

1
College of Forestry, Shanxi Agricultural University, Taigu 030801, China
2
State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, 51 Hexing Road, Harbin 150040, China
*
Author to whom correspondence should be addressed.
Forests 2019, 10(8), 688; https://doi.org/10.3390/f10080688
Received: 27 June 2019 / Revised: 31 July 2019 / Accepted: 13 August 2019 / Published: 14 August 2019
PDF [2287 KB, uploaded 14 August 2019]

Abstract

Research Highlights: Sequence phylogeny, genome organisation, gene structure, conserved motifs, promoter cis-element and expression profiling of poplar NACs related to salt stress were detected. In addition, expression of two salt-induced NACs was analysed. Background and Objectives: NAC transcription factor (TF) proteins are involved in a wide range of functions during plant development and stress-related endurance processes. To understand the function of Populus NAC TFs in salt stress tolerance, we characterised the structure and expression profile of a total of 289 NAC members. Materials and Methods: Sequence phylogeny, genome organisation, gene structure, motif composition and promoter cis-element were detected using bioinformatics. The expression pattern of Populus NAC TFs under salt stress was also detected using RNA-Seq and RT-qPCR. Results: Synteny analysis showed that 46 and 37 Populus NAC genes were involved in whole-genome duplication and tandem duplication events, respectively. The expression pattern of Populus NAC TFs under salt stress showed the expression of the 289 PtNACs of 84K poplar was induced. Similar expression trends of NACs were found in Populus simonii × P. nigra T. S. Hwang et Liang and Arabidopsis thaliana (L.) Heynh. Conclusions: The correlation analysis showed that the expression of two differentially expressed NAC genes PtNAC024 and PtNAC182 was significantly associated with most of the 63 differentially expressed genes tested. The expression of PtNAC024 and PtNAC182 in different tissues was also analysed in silico and different expression patterns were found. Together, this study provides a solid basis to explore stress-related NAC TF functions in Populus salt tolerance and development.
Keywords: NAC; transcription factor; salt stress; gene express; Populus NAC; transcription factor; salt stress; gene express; Populus
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Wang, S.; Huang, J.; Wang, X.; Dang, H.; Jiang, T.; Han, Y. Expression Analysis of the NAC Transcription Factor Family of Populus in Response to Salt Stress. Forests 2019, 10, 688.

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