Pathological Impairment, Cell Cycle Arrest and Apoptosis of Thymus and Bursa of Fabricius Induced by Aflatoxin-Contaminated Corn in Broilers
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals, Diet, and Study Design
2.2. Pathological Observation
2.3. Cell Cycle Phase Detection by Flow Cytometry
2.4. Annexin V Apoptosis Detection by Flow Cytometry
3. Results
3.1. Histopathological Lesions
3.2. Cell Cycle Phase Distribution of Immune Organs
3.3. Annexin V-FITC Staining Assay by Flow Cytometry
4. Discussion
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Diet 1 | Control | 25% | 50% | 75% | 100% | Control Corn | Contaminated Corn |
---|---|---|---|---|---|---|---|
1–21 days | |||||||
AFB1 | ND 2 | 16.3 | 36.9 | 45.6 | 82.4 | ND | 149.6 |
AFB2 | ND | 3.15 | 6.38 | 7.86 | 14.2 | ND | 24.2 |
22–42 days | |||||||
AFB1 | ND | 34.3 | 69.3 | 95.2 | 134 | ND | 229 |
AFB2 | ND | 6.17 | 12.1 | 17 | 23.6 | ND | 37.8 |
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Peng, X.; Bai, S.; Ding, X.; Zhang, K. Pathological Impairment, Cell Cycle Arrest and Apoptosis of Thymus and Bursa of Fabricius Induced by Aflatoxin-Contaminated Corn in Broilers. Int. J. Environ. Res. Public Health 2017, 14, 77. https://doi.org/10.3390/ijerph14010077
Peng X, Bai S, Ding X, Zhang K. Pathological Impairment, Cell Cycle Arrest and Apoptosis of Thymus and Bursa of Fabricius Induced by Aflatoxin-Contaminated Corn in Broilers. International Journal of Environmental Research and Public Health. 2017; 14(1):77. https://doi.org/10.3390/ijerph14010077
Chicago/Turabian StylePeng, Xi, Shiping Bai, Xuemei Ding, and Keying Zhang. 2017. "Pathological Impairment, Cell Cycle Arrest and Apoptosis of Thymus and Bursa of Fabricius Induced by Aflatoxin-Contaminated Corn in Broilers" International Journal of Environmental Research and Public Health 14, no. 1: 77. https://doi.org/10.3390/ijerph14010077