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Volume 45
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10.3390/medicina45050047
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Medicina is published by MDPI from Volume 54 Issue 1 (2018). Previous articles were published by another publisher in Open Access under a CC-BY (or CC-BY-NC-ND) licence, and they are hosted by MDPI on mdpi.com as a courtesy and upon agreement with Lithuanian Medical Association, Lithuanian University of Health Sciences (LUHS), and Vilnius University.
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Article

Enhancement of photodynamic tumor therapy effectiveness by electroporation in vitro

by
Jūratė Labanauskienė
1,*,
Saulius Šatkauskas
2,
Vida Kirvelienė
3,
Mindaugas Venslauskas
2,
Vydmantas Atkočius
1 and
Janina Didžiapetrienė
1
1
Scientific Research Center, Institute of Oncology, Vilnius University
2
Department of Biology, Vytautas Magnus University
3
Department of Biochemistry and Biophysics, Vilnius University, Lithuania
*
Author to whom correspondence should be addressed.
Medicina 2009, 45(5), 372; https://doi.org/10.3390/medicina45050047
Submission received: 17 November 2008 / Accepted: 4 May 2009 / Published: 9 May 2009
Versions Notes

Abstract

The aim of our study was to determine if electroporation could improve the efficacy of photodynamic tumor therapy. A disadvantage of photodynamic therapy is a slow and in some cases insufficient accumulation of photosensitizer in tumor tissue, which could restrict the achievement of an efficient dose. Under the action of electric pulses, cells undergo membrane electroporation, which results in an increased permeability to various exogenous molecules. In this study, murine hepatoma MH22A cells were exposed to light in vitro in the presence of a photosensitizer, either chlorin e6 or aluminum phthalocyanine tetrasulfonate, following electroporation. Accumulation of the photosensitizers was registered by fluorescence microscopy. Cell viability was determined by the MTT assay. Our results demonstrate that electroporation improves an access of chlorin e6 and aluminum phthalocyanine tetrasulfonate to MH22A cells. Electroporation in combination with photosensitization significantly reduces viability of the treated cells even at low doses of photosensitizers.
Keywords: electroporation; photodynamic tumor therapy; chlorin e6; aluminum phthalocyanine tetrasulfonate electroporation; photodynamic tumor therapy; chlorin e6; aluminum phthalocyanine tetrasulfonate

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MDPI and ACS Style

Labanauskienė, J.; Šatkauskas, S.; Kirvelienė, V.; Venslauskas, M.; Atkočius, V.; Didžiapetrienė, J. Enhancement of photodynamic tumor therapy effectiveness by electroporation in vitro. Medicina 2009, 45, 372. https://doi.org/10.3390/medicina45050047

AMA Style

Labanauskienė J, Šatkauskas S, Kirvelienė V, Venslauskas M, Atkočius V, Didžiapetrienė J. Enhancement of photodynamic tumor therapy effectiveness by electroporation in vitro. Medicina. 2009; 45(5):372. https://doi.org/10.3390/medicina45050047

Chicago/Turabian Style

Labanauskienė, Jūratė, Saulius Šatkauskas, Vida Kirvelienė, Mindaugas Venslauskas, Vydmantas Atkočius, and Janina Didžiapetrienė. 2009. "Enhancement of photodynamic tumor therapy effectiveness by electroporation in vitro" Medicina 45, no. 5: 372. https://doi.org/10.3390/medicina45050047

APA Style

Labanauskienė, J., Šatkauskas, S., Kirvelienė, V., Venslauskas, M., Atkočius, V., & Didžiapetrienė, J. (2009). Enhancement of photodynamic tumor therapy effectiveness by electroporation in vitro. Medicina, 45(5), 372. https://doi.org/10.3390/medicina45050047

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