Next Article in Journal
Effect of High Temperature and Drought on the Fatty Acid Composition of Oil of Different Linseed Varieties and Its Association with Gene Expression
Previous Article in Journal
Special Issue “Machine Learning and Bioinformatics in Human Health and Disease 2nd Edition”
Previous Article in Special Issue
IKKβ Kinase Promotes Stemness, Migration, and Invasion in KRAS-Driven Lung Adenocarcinoma Cells
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
IJMS
Volume 27
Issue 18
10.3390/ijms27188004
ijms-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
Correction to Int. J. Mol. Sci. 2020, 21(16), 5806.
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Correction

Correction: Rodrigues et al. IKKβ Kinase Promotes Stemness, Migration, and Invasion in KRAS-Driven Lung Adenocarcinoma Cells. Int. J. Mol. Sci. 2020, 21, 5806

by
Felipe Silva Rodrigues
1,2,†,
Vanessa Silva Miranda
1,
Tatiana Correa Carneiro-Lobo
1,
Luiza Coimbra Scalabrini
1,
Björn Kruspig
2,
Elena Levantini
3,4,
Daniel J. Murphy
2,5 and
Daniela Sanchez Bassères
1,*
1
Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo 05508-000, Brazil
2
Institute of Cancer Sciences, University of Glasgow, Glasgow G61 1QH, UK
3
Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02115, USA
4
Istituto di Tecnologie Biomediche, Consiglio Nazionale dele Ricerche, 56124 Pisa, Italy
5
Cancer Research UK Beatson Institute, Glasgow G61 1BD, UK
*
Author to whom correspondence should be addressed.
†
Current address: Tumour-Host Interaction Laboratory, The Francis Crick Institute, London NW1 1AT, UK.
Int. J. Mol. Sci. 2026, 27(18), 8004; https://doi.org/10.3390/ijms27188004
Submission received: 11 August 2026 / Accepted: 17 August 2026 / Published: 9 September 2026
(This article belongs to the Special Issue Cancer Cell Reprogramming)
Browse Figure
Versions Notes
Error in Figure
In the original publication [1], the Western blot images for phospho-IκBα, IκBα, and β-actin in Figure 2A were inadvertently duplicated from a separate publication. Because the experiments in both studies used identical cell lines, treatments, antibodies, and protocols, the same immunoblot image was mistakenly included during figure assembly. This panel serves to confirm that Compound A treatment inhibits IKKβ kinase activity, as demonstrated by us and others. The error occurred during figure preparation. The experiments were independently performed using identical protocols, and the results are consistent with those reported in the manuscript. A corrected Figure 2A, containing immunoblot images from the independent experiment, is provided below.
The authors state that the scientific conclusions are unaffected. This correction was approved by the Academic Editor. The original publication has also been updated.

Reference

  1. Rodrigues, F.S.; Miranda, V.S.; Carneiro-Lobo, T.C.; Scalabrini, L.C.; Kruspig, B.; Levantini, E.; Murphy, D.J.; Bassères, D.S. IKKβ Kinase Promotes Stemness, Migration, and Invasion in KRAS-Driven Lung Adenocarcinoma Cells. Int. J. Mol. Sci. 2020, 21, 5806. [Google Scholar] [CrossRef] [Scilit] [PubMed]
Ijms 27 08004 g002
Figure 2. IKKβ targeting reduces the expression of stemness-associated genes in KRAS-mutant lung cancer cells. (A) Western blotting of A549 and H358 cells treated with 0.1% DMSO or 5 µM Compound A (CmpdA) for 30 min. Antibodies were used as indicated. Nitrocellulose membrane was cut before probing with the respective primary antibody, and full membrane blots are presented in Figure S2. The representative Western blots are shown. The protein bands were quantitated and normalized to the reference samples (0.1% DMSO-treated samples) using Image J software (version 1.51). (B) A549 and H358 cells were treated with 0.1% DMSO or the indicated concentrations of CmpdA for 48 h, and expression of SOX2, OCT4, NANOG, BMI1, and CXCR4 was evaluated by qRT-PCR using β-ACTIN as an endogenous control. The bar graphs represent average ±1 SD of three independent experiments (n = 3). Statistical significance was determined by one-way ANOVA with the post hoc Turkey test. (* p < 0.5, ** p < 0.01, *** p < 0.001, **** p < 0.0001) by comparing the CmpdA-treated groups with the DMSO-treated group.
Figure 2. IKKβ targeting reduces the expression of stemness-associated genes in KRAS-mutant lung cancer cells. (A) Western blotting of A549 and H358 cells treated with 0.1% DMSO or 5 µM Compound A (CmpdA) for 30 min. Antibodies were used as indicated. Nitrocellulose membrane was cut before probing with the respective primary antibody, and full membrane blots are presented in Figure S2. The representative Western blots are shown. The protein bands were quantitated and normalized to the reference samples (0.1% DMSO-treated samples) using Image J software (version 1.51). (B) A549 and H358 cells were treated with 0.1% DMSO or the indicated concentrations of CmpdA for 48 h, and expression of SOX2, OCT4, NANOG, BMI1, and CXCR4 was evaluated by qRT-PCR using β-ACTIN as an endogenous control. The bar graphs represent average ±1 SD of three independent experiments (n = 3). Statistical significance was determined by one-way ANOVA with the post hoc Turkey test. (* p < 0.5, ** p < 0.01, *** p < 0.001, **** p < 0.0001) by comparing the CmpdA-treated groups with the DMSO-treated group.
Ijms 27 08004 g002
Disclaimer/Publisher’s Note: The statements, opinions and data contained in all publications are solely those of the individual author(s) and contributor(s) and not of MDPI and/or the editor(s). MDPI and/or the editor(s) disclaim responsibility for any injury to people or property resulting from any ideas, methods, instructions or products referred to in the content.

Share and Cite

MDPI and ACS Style

Rodrigues, F.S.; Miranda, V.S.; Carneiro-Lobo, T.C.; Scalabrini, L.C.; Kruspig, B.; Levantini, E.; Murphy, D.J.; Bassères, D.S. Correction: Rodrigues et al. IKKβ Kinase Promotes Stemness, Migration, and Invasion in KRAS-Driven Lung Adenocarcinoma Cells. Int. J. Mol. Sci. 2020, 21, 5806. Int. J. Mol. Sci. 2026, 27, 8004. https://doi.org/10.3390/ijms27188004

AMA Style

Rodrigues FS, Miranda VS, Carneiro-Lobo TC, Scalabrini LC, Kruspig B, Levantini E, Murphy DJ, Bassères DS. Correction: Rodrigues et al. IKKβ Kinase Promotes Stemness, Migration, and Invasion in KRAS-Driven Lung Adenocarcinoma Cells. Int. J. Mol. Sci. 2020, 21, 5806. International Journal of Molecular Sciences. 2026; 27(18):8004. https://doi.org/10.3390/ijms27188004

Chicago/Turabian Style

Rodrigues, Felipe Silva, Vanessa Silva Miranda, Tatiana Correa Carneiro-Lobo, Luiza Coimbra Scalabrini, Björn Kruspig, Elena Levantini, Daniel J. Murphy, and Daniela Sanchez Bassères. 2026. "Correction: Rodrigues et al. IKKβ Kinase Promotes Stemness, Migration, and Invasion in KRAS-Driven Lung Adenocarcinoma Cells. Int. J. Mol. Sci. 2020, 21, 5806" International Journal of Molecular Sciences 27, no. 18: 8004. https://doi.org/10.3390/ijms27188004

APA Style

Rodrigues, F. S., Miranda, V. S., Carneiro-Lobo, T. C., Scalabrini, L. C., Kruspig, B., Levantini, E., Murphy, D. J., & Bassères, D. S. (2026). Correction: Rodrigues et al. IKKβ Kinase Promotes Stemness, Migration, and Invasion in KRAS-Driven Lung Adenocarcinoma Cells. Int. J. Mol. Sci. 2020, 21, 5806. International Journal of Molecular Sciences, 27(18), 8004. https://doi.org/10.3390/ijms27188004

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop