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Article

MicroRNA-193a-3p as a Valuable Biomarker for Discriminating between Colorectal Cancer and Colorectal Adenoma Patients

by
Marija Fabijanec
1,
Andrea Hulina-Tomašković
2,*,
Mario Štefanović
2,3,
Donatella Verbanac
2,
Ivana Ćelap
2,3,
Anita Somborac-Bačura
2,
Marija Grdić Rajković
2,
Alma Demirović
4,
Snježana Ramić
5,
Božo Krušlin
4,
Lada Rumora
2,
Andrea Čeri
2,
Martha Koržinek
2,
József Petrik
2,
Neven Ljubičić
6,
Neven Baršić
6 and
Karmela Barišić
2
1
Center for Applied Medical Biochemistry, Faculty of Pharmacy and Biochemistry, University of Zagreb, 10000 Zagreb, Croatia
2
Department of Medical Biochemistry and Hematology, Faculty of Pharmacy and Biochemistry, University of Zagreb, 10000 Zagreb, Croatia
3
Department of Clinical Chemistry, Sestre Milosrdnice University Hospital Center, 10000 Zagreb, Croatia
4
Department of Pathology and Cytology “Ljudevit Jurak”, Sestre Milosrdnice University Hospital Center, 10000 Zagreb, Croatia
5
Department of Oncological Pathology, University Hospital for Tumors, Sestre Milosrdnice University Hospital Centre, 10000 Zagreb, Croatia
6
Department of Internal Medicine, Division of Gastroenterology and Hepatology, Sestre Milosrdnice University Hospital Center, 10000 Zagreb, Croatia
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2024, 25(15), 8156; https://doi.org/10.3390/ijms25158156
Submission received: 24 June 2024 / Revised: 16 July 2024 / Accepted: 22 July 2024 / Published: 26 July 2024
(This article belongs to the Special Issue New Insights into Colorectal Cancer)
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Abstract

Specific markers for colorectal cancer (CRC), preceded by colorectal adenoma (pre-CRC), are lacking. This study aimed to investigate whether microRNAs (miR-19a-3p, miR-92a-3p, miR-193a-3p, and miR-210-3p) from tissues and exosomes are potential CRC biomarkers and compare them to existing biomarkers, namely carcinoembryonic antigen (CEA) and carbohydrate antigen (CA) 19-9. MiRNA was isolated in the samples of 52 CRC and 76 pre-CRC patients. Expression levels were analyzed by RT-qPCR. When comparing pre-CRC and CRC tissue expression levels, only miR-193a-3p showed statistically significant result (p < 0.0001). When comparing the tissues and exosomes of CRC samples, a statistically significant difference was found for miR-193a-3p (p < 0.0001), miR-19a-3p (p < 0.0001), miR-92a-3p (p = 0.0212), and miR-210-3p (p < 0.0001). A receiver-operating characteristic (ROC) curve and area under the ROC curve (AUC) were used to evaluate the diagnostic value of CEA, CA 19-9, and miRNAs. CEA and CA 19-9 had good diagnostic values (AUCs of 0.798 and 0.668). The diagnostic value only of miR-193a-3p was highlighted (AUC = 0.725). The final logistic regression model, in which we put a combination of CEA concentration and the miR-193a-3p expression level in tissues, showed that using these two markers can distinguish CRC and pre-CRC in 71.3% of cases (AUC = 0.823). MiR-193a-3p from tissues could be a potential CRC biomarker.
Keywords: colorectal cancer; colorectal adenoma; colonoscopy; liquid biopsy; CEA; CA 19-9; microRNA; miR-193a colorectal cancer; colorectal adenoma; colonoscopy; liquid biopsy; CEA; CA 19-9; microRNA; miR-193a

Share and Cite

MDPI and ACS Style

Fabijanec, M.; Hulina-Tomašković, A.; Štefanović, M.; Verbanac, D.; Ćelap, I.; Somborac-Bačura, A.; Grdić Rajković, M.; Demirović, A.; Ramić, S.; Krušlin, B.; et al. MicroRNA-193a-3p as a Valuable Biomarker for Discriminating between Colorectal Cancer and Colorectal Adenoma Patients. Int. J. Mol. Sci. 2024, 25, 8156. https://doi.org/10.3390/ijms25158156

AMA Style

Fabijanec M, Hulina-Tomašković A, Štefanović M, Verbanac D, Ćelap I, Somborac-Bačura A, Grdić Rajković M, Demirović A, Ramić S, Krušlin B, et al. MicroRNA-193a-3p as a Valuable Biomarker for Discriminating between Colorectal Cancer and Colorectal Adenoma Patients. International Journal of Molecular Sciences. 2024; 25(15):8156. https://doi.org/10.3390/ijms25158156

Chicago/Turabian Style

Fabijanec, Marija, Andrea Hulina-Tomašković, Mario Štefanović, Donatella Verbanac, Ivana Ćelap, Anita Somborac-Bačura, Marija Grdić Rajković, Alma Demirović, Snježana Ramić, Božo Krušlin, and et al. 2024. "MicroRNA-193a-3p as a Valuable Biomarker for Discriminating between Colorectal Cancer and Colorectal Adenoma Patients" International Journal of Molecular Sciences 25, no. 15: 8156. https://doi.org/10.3390/ijms25158156

APA Style

Fabijanec, M., Hulina-Tomašković, A., Štefanović, M., Verbanac, D., Ćelap, I., Somborac-Bačura, A., Grdić Rajković, M., Demirović, A., Ramić, S., Krušlin, B., Rumora, L., Čeri, A., Koržinek, M., Petrik, J., Ljubičić, N., Baršić, N., & Barišić, K. (2024). MicroRNA-193a-3p as a Valuable Biomarker for Discriminating between Colorectal Cancer and Colorectal Adenoma Patients. International Journal of Molecular Sciences, 25(15), 8156. https://doi.org/10.3390/ijms25158156

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