Next Article in Journal
Recent Advances on the Role of Brain-Derived Neurotrophic Factor (BDNF) in Neurodegenerative Diseases
Next Article in Special Issue
Short-Term Mild Hypoxia Modulates Na,K-ATPase to Maintain Membrane Electrogenesis in Rat Skeletal Muscle
Previous Article in Journal
Orchid Biochemistry 2.0
Previous Article in Special Issue
High-Fat Diet Impairs Muscle Function and Increases the Risk of Environmental Heatstroke in Mice
 
 
Article
Peer-Review Record

Effects of Maca on Muscle Hypertrophy in C2C12 Skeletal Muscle Cells

Int. J. Mol. Sci. 2022, 23(12), 6825; https://doi.org/10.3390/ijms23126825
by Dong Yi, Maki Yoshikawa, Takeshi Sugimoto, Keigo Tomoo, Yoko Okada and Takeshi Hashimoto *
Reviewer 1:
Reviewer 2:
Int. J. Mol. Sci. 2022, 23(12), 6825; https://doi.org/10.3390/ijms23126825
Submission received: 2 June 2022 / Revised: 14 June 2022 / Accepted: 16 June 2022 / Published: 19 June 2022
(This article belongs to the Special Issue Advances in Skeletal Muscle Function and Metabolism)

Round 1

Reviewer 1 Report

The revised version is satisfactory and I have only one comment concerning the experiment shown in Fig. 4: translation efficiency is determined by the incorporation of OPP into newly synthesized proteins, there are commercial kits to measure translation efficiency (Click-iT protein synthesis assay kit).  The method used in Figure 4 is old and inaccurate. Moreover, for muscle cell culture, a bulk protein extract is inaccurate, as differences in translation efficiency are found between fused and non-fused cells (https://doi.org/10.1093/gerona/glac058).

I suggest the authors removing Fig. 4 and the statements related to this Figure (better less and more accurate data).

Author Response

We are so grateful to the reviewer's valuable comments.

Given the comments from the reviewer, we have deleted the data of puromycin expression and related description. 

Thank you very much again.

Reviewer 2 Report

The authors followed all of my suggestions and the manuscript in its current form is suitable for publication in this journal. 

Author Response

We are so grateful to the reviewer's valuable comments. We have improved our manuscript. Thank you very much again.

Back to TopTop