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Volume 22
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10.3390/ijms221910200
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Article

The Preeclamptic Environment Promotes the Activation of Transcription Factor Kappa B by P53/RSK1 Complex in a HTR8/SVneo Trophoblastic Cell Line

by
Agata Sakowicz
1,*,
Michalina Bralewska
1,
Tadeusz Pietrucha
1,
Francesc Figueras
2,
Dominika E. Habrowska-Górczyńska
3,
Agnieszka W. Piastowska-Ciesielska
3,
Agnieszka Gach
4,
Bartosz Sakowicz
5,
Magda Rybak-Krzyszkowska
6,
Hubert Huras
6,
Mariusz Grzesiak
7,8 and
Lidia Biesiada
9
1
Department of Medical Biotechnology, Medical University of Lodz, 90-752 Lodz, Poland
2
Barcelona Center of Maternal-Fetal Medicine and Neonatology (Hospital Clinic and Hospital Sant Joan de Deu), IDIBAPS, University of Barcelona, 08036 Barcelona, Spain
3
Department of Cell Culture and Genomic Analysis, Medical University of Lodz, 90-752 Lodz, Poland
4
Department of Genetics, Polish Mother’s Memorial Hospital-Research Institute in Lodz, 93-338 Lodz, Poland
5
Department of Microelectronics and Computer Science, Lodz University of Technology, 93-005 Lodz, Poland
6
Department of Obstetrics and Perinatology, University Hospital in Krakow, 31-501 Krakow, Poland
7
Department of Perinatology, Obstetrics and Gynecology, Polish Mother’s Memorial Hospital-Research Institute in Lodz, 93-338 Lodz, Poland
8
Department of Obstetrics and Gynecology, Medical University of Lodz, 93-338 Lodz, Poland
9
Department of Obstetrics and Gynecology, Polish Mother’s Memorial Hospital-Research Institute, 93-338 Lodz, Poland
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2021, 22(19), 10200; https://doi.org/10.3390/ijms221910200
Received: 18 July 2021 / Revised: 13 August 2021 / Accepted: 17 September 2021 / Published: 22 September 2021
(This article belongs to the Special Issue NF-κB and Disease 2.0)
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Abstract

Preeclampsia is a pregnancy disorder associated with shallow placentation, forcing placental cells to live in hypoxic conditions. This activates the transcription factor kappa B (NFκB) in maternal and placental cells. Although the role of NFκB in preeclampsia is well documented, its mechanism of activation in trophoblastic cells has been never studied. This study investigates the mechanism of NFκB activation in a first trimester trophoblastic cell line (HTR8/SVneo) stimulated by a medium containing serum from preeclamptic (PE) or normotensive (C) women in hypoxic (2% O2) or normoxic (8% O2) conditions. The results indicate that in HTR8/SVneo cells, the most widely studied NFκB pathways, i.e., canonical, non-canonical and atypical, are downregulated in environment PE 2% O2 in comparison to C 8% O2. Therefore, other pathways may be responsible for NFκB activation. One such pathway depends on the activation of NFκB by the p53/RSK1 complex through its phosphorylation at Serine 536 (pNFκB Ser536). The data generated by our study show that inhibition of the p53/RSK1 pathway by p53-targeted siRNA results in a depletion of pNFκB Ser536 in the nucleus, but only in cells incubated with PE serum at 2% O2. Thus, the p53/RSK1 complex might play a critical role in the activation of NFκB in trophoblastic cells and preeclamptic placentas. View Full-Text
Keywords: NFκB activation pathways; preeclampsia; p53/RSK1 complex; trophoblastic cell line NFκB activation pathways; preeclampsia; p53/RSK1 complex; trophoblastic cell line
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MDPI and ACS Style

Sakowicz, A.; Bralewska, M.; Pietrucha, T.; Figueras, F.; Habrowska-Górczyńska, D.E.; Piastowska-Ciesielska, A.W.; Gach, A.; Sakowicz, B.; Rybak-Krzyszkowska, M.; Huras, H.; Grzesiak, M.; Biesiada, L. The Preeclamptic Environment Promotes the Activation of Transcription Factor Kappa B by P53/RSK1 Complex in a HTR8/SVneo Trophoblastic Cell Line. Int. J. Mol. Sci. 2021, 22, 10200. https://doi.org/10.3390/ijms221910200

AMA Style

Sakowicz A, Bralewska M, Pietrucha T, Figueras F, Habrowska-Górczyńska DE, Piastowska-Ciesielska AW, Gach A, Sakowicz B, Rybak-Krzyszkowska M, Huras H, Grzesiak M, Biesiada L. The Preeclamptic Environment Promotes the Activation of Transcription Factor Kappa B by P53/RSK1 Complex in a HTR8/SVneo Trophoblastic Cell Line. International Journal of Molecular Sciences. 2021; 22(19):10200. https://doi.org/10.3390/ijms221910200

Chicago/Turabian Style

Sakowicz, Agata, Michalina Bralewska, Tadeusz Pietrucha, Francesc Figueras, Dominika E. Habrowska-Górczyńska, Agnieszka W. Piastowska-Ciesielska, Agnieszka Gach, Bartosz Sakowicz, Magda Rybak-Krzyszkowska, Hubert Huras, Mariusz Grzesiak, and Lidia Biesiada. 2021. "The Preeclamptic Environment Promotes the Activation of Transcription Factor Kappa B by P53/RSK1 Complex in a HTR8/SVneo Trophoblastic Cell Line" International Journal of Molecular Sciences 22, no. 19: 10200. https://doi.org/10.3390/ijms221910200

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