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Article

Umbilical Cord Mesenchymal Stromal Cell-Derived Exosomes Rescue the Loss of Outer Hair Cells and Repair Cochlear Damage in Cisplatin-Injected Mice

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Department of Otolaryngology, Tungs’ Taichung Metroharbor Hospital, Taichung 435, Taiwan
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Rong Hsing Research Center for Translational Medicine, National Chung Hsing University, 145, Guoguang Rd., South Dist., Taichung City 402, Taiwan
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Department of Medical Research, Mackay Memorial Hospital, Taipei 104, Taiwan
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Department of Otolaryngology, Mackay Memorial Hospital, New Taipei City 251, Taiwan
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Department of Medical Research, Tungs’ Taichung Metroharbor Hospital, Taichung 435, Taiwan
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Graduate Institute of Biomedical Sciences, China Medical University, Taichung 406, Taiwan
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General Education Center, Jen-Teh Junior College of Medicine, Nursing and Management, Miaoli 356, Taiwan
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Department of Thoracic Surgery, Chung Shan Medical University Hospital, Taichung 402, Taiwan
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School of Medicine, Chung Shan Medical University, Taichung 402, Taiwan
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Center for Molecular Medicine, China Medical University Hospital, Taichung 406, Taiwan
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Department of Medical Laboratory and Biotechnology, Asia University, Taichung 413, Taiwan
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Institute of Biomedical Sciences, Mackay Medical College, New Taipei City 252, Taiwan
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Laboratory Animal Service Center, Office of Research and Development, China Medical University, Taichung 406, Taiwan
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Medical and Pharmaceutical Industry Technology and Development Center, New Taipei City 248, Taiwan
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Department of Audiology and Speech-Language Pathology, Mackay Medical College, New Taipei City 252, Taiwan
*
Author to whom correspondence should be addressed.
Academic Editor: Joan Oliva
Int. J. Mol. Sci. 2021, 22(13), 6664; https://doi.org/10.3390/ijms22136664
Received: 15 May 2021 / Revised: 10 June 2021 / Accepted: 12 June 2021 / Published: 22 June 2021
Umbilical cord-derived mesenchymal stromal cells (UCMSCs) have potential applications in regenerative medicine. UCMSCs have been demonstrated to repair tissue damage in many inflammatory and degenerative diseases. We have previously shown that UCMSC exosomes reduce nerve injury-induced pain in rats. In this study, we characterized UCMSC exosomes using RNA sequencing and proteomic analyses and investigated their protective effects on cisplatin-induced hearing loss in mice. Two independent experiments were designed to investigate the protective effects on cisplatin-induced hearing loss in mice: (i) chronic intraperitoneal cisplatin administration (4 mg/kg) once per day for 5 consecutive days and intraperitoneal UCMSC exosome (1.2 μg/μL) injection at the same time point; and (ii) UCMSC exosome (1.2 μg/μL) injection through a round window niche 3 days after chronic cisplatin administration. Our data suggest that UCMSC exosomes exert protective effects in vivo. The post-traumatic administration of UCMSC exosomes significantly improved hearing loss and rescued the loss of cochlear hair cells in mice receiving chronic cisplatin injection. Neuropathological gene panel analyses further revealed the UCMSC exosomes treatment led to beneficial changes in the expression levels of many genes in the cochlear tissues of cisplatin-injected mice. In conclusion, UCMSC exosomes exerted protective effects in treating ototoxicity-induced hearing loss by promoting tissue remodeling and repair. View Full-Text
Keywords: mesenchymal stromal cells; cisplatin; hair cells; inner ear; ototoxicity; extracellular vesicles; exosomes mesenchymal stromal cells; cisplatin; hair cells; inner ear; ototoxicity; extracellular vesicles; exosomes
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MDPI and ACS Style

Tsai, S.C.-S.; Yang, K.D.; Chang, K.-H.; Lin, F.C.-F.; Chou, R.-H.; Li, M.-C.; Cheng, C.-C.; Kao, C.-Y.; Chen, C.-P.; Lin, H.-C.; Hsu, Y.-C. Umbilical Cord Mesenchymal Stromal Cell-Derived Exosomes Rescue the Loss of Outer Hair Cells and Repair Cochlear Damage in Cisplatin-Injected Mice. Int. J. Mol. Sci. 2021, 22, 6664. https://doi.org/10.3390/ijms22136664

AMA Style

Tsai SC-S, Yang KD, Chang K-H, Lin FC-F, Chou R-H, Li M-C, Cheng C-C, Kao C-Y, Chen C-P, Lin H-C, Hsu Y-C. Umbilical Cord Mesenchymal Stromal Cell-Derived Exosomes Rescue the Loss of Outer Hair Cells and Repair Cochlear Damage in Cisplatin-Injected Mice. International Journal of Molecular Sciences. 2021; 22(13):6664. https://doi.org/10.3390/ijms22136664

Chicago/Turabian Style

Tsai, Stella C.-S., Kuender D. Yang, Kuang-Hsi Chang, Frank C.-F. Lin, Ruey-Hwang Chou, Min-Chih Li, Ching-Chang Cheng, Chien-Yu Kao, Chie-Pein Chen, Hung-Ching Lin, and Yi-Chao Hsu. 2021. "Umbilical Cord Mesenchymal Stromal Cell-Derived Exosomes Rescue the Loss of Outer Hair Cells and Repair Cochlear Damage in Cisplatin-Injected Mice" International Journal of Molecular Sciences 22, no. 13: 6664. https://doi.org/10.3390/ijms22136664

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