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Does Protein Glycation Impact on the Drought-Related Changes in Metabolism and Nutritional Properties of Mature Pea (Pisum sativum L.) Seeds?

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Department of Biochemistry, St. Petersburg State University, 199004 St. Petersburg, Russia
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Department of Bioorganic Chemistry, Leibniz Institute of Plant Biochemistry, 06120 Halle, Germany
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Department of Plant Physiology and Biochemistry, St. Petersburg State University, 199034 St. Petersburg, Russia
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Institute of Chemistry – Food Chemistry, Martin-Luther University Halle-Wittenberg, 06120 Halle, Germany
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Research Institute of Hygiene, Occupational Pathology and Human Ecology, 188663 Leningrad Oblast, Russia
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Postovsky Institute of Organic Synthesis of Ural Division of Russian Academy of Sciences, 620137 Yekaterinburg, Russia
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Department of Metabolic and Cell Biology, Leibniz Institute of Plant Biochemistry, 06120 Halle, Germany
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Department of Pharmaceutical Chemistry and Bioanalytics, Institute of Pharmacy, Martin-Luther University Halle-Wittenberg, 06120 Halle, Germany
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All-Russia Research Institute for Agricultural Microbiology, 196608 St. Petersburg, Russia
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Department of Genetics and Biotechnology, St. Petersburg State University, 199034 St. Petersburg, Russia
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(2), 567; https://doi.org/10.3390/ijms21020567
Received: 10 December 2019 / Revised: 1 January 2020 / Accepted: 3 January 2020 / Published: 15 January 2020
(This article belongs to the Special Issue Plant Protein Glycation)
Protein glycation is usually referred to as an array of non-enzymatic post-translational modifications formed by reducing sugars and carbonyl products of their degradation. The resulting advanced glycation end products (AGEs) represent a heterogeneous group of covalent adducts, known for their pro-inflammatory effects in mammals, and impacting on pathogenesis of metabolic diseases and ageing. In plants, AGEs are the markers of tissue ageing and response to environmental stressors, the most prominent of which is drought. Although water deficit enhances protein glycation in leaves, its effect on seed glycation profiles is still unknown. Moreover, the effect of drought on biological activities of seed protein in mammalian systems is still unstudied with respect to glycation. Therefore, here we address the effects of a short-term drought on the patterns of seed protein-bound AGEs and accompanying alterations in pro-inflammatory properties of seed protein in the context of seed metabolome dynamics. A short-term drought, simulated as polyethylene glycol-induced osmotic stress and applied at the stage of seed filling, resulted in the dramatic suppression of primary seed metabolism, although the secondary metabolome was minimally affected. This was accompanied with significant suppression of NF-kB activation in human SH-SY5Y neuroblastoma cells after a treatment with protein hydrolyzates, isolated from the mature seeds of drought-treated plants. This effect could not be attributed to formation of known AGEs. Most likely, the prospective anti-inflammatory effect of short-term drought is related to antioxidant effect of unknown secondary metabolite protein adducts, or down-regulation of unknown plant-specific AGEs due to suppression of energy metabolism during seed filling. View Full-Text
Keywords: advanced glycation end products (AGEs); drought; glycation; SH-SY5Y human neuroblastoma cells; metabolomics; osmotic stress; pea (Pisum sativum L.); pro-inflammatory; seeds; seed metabolism; signaling pathways advanced glycation end products (AGEs); drought; glycation; SH-SY5Y human neuroblastoma cells; metabolomics; osmotic stress; pea (Pisum sativum L.); pro-inflammatory; seeds; seed metabolism; signaling pathways
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Leonova, T.; Popova, V.; Tsarev, A.; Henning, C.; Antonova, K.; Rogovskaya, N.; Vikhnina, M.; Baldensperger, T.; Soboleva, A.; Dinastia, E.; Dorn, M.; Shiroglasova, O.; Grishina, T.; Balcke, G.U.; Ihling, C.; Smolikova, G.; Medvedev, S.; Zhukov, V.A.; Babakov, V.; Tikhonovich, I.A.; Glomb, M.A.; Bilova, T.; Frolov, A. Does Protein Glycation Impact on the Drought-Related Changes in Metabolism and Nutritional Properties of Mature Pea (Pisum sativum L.) Seeds? Int. J. Mol. Sci. 2020, 21, 567.

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