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Mass Spectrometry-Based Metabolomics Analysis of Obese Patients’ Blood Plasma

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Institute of Biomedical Chemistry, 10 Building 8, Pogodinskaya Street, 119121 Moscow, Russia
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Metabometrics Inc, 651 N Broad St, Suite 205 #1370, Middletown, DE 19709, USA
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Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(2), 568; https://doi.org/10.3390/ijms21020568
Received: 25 November 2019 / Revised: 9 January 2020 / Accepted: 14 January 2020 / Published: 15 January 2020
(This article belongs to the Special Issue Metabolomics in Medicine)
Scientists currently use only a small portion of the information contained in the blood metabolome. The identification of metabolites is a huge challenge because only highly abundant and well-separated compounds can be easily identified in complex samples. However, new approaches that enhance the identification of compounds have emerged; among them, the identification of compounds based on their involvement in a particular biological context is a recent development. In this work, this approach was first applied to identify metabolites in complex samples and, together with metabolite set enrichment analysis, was used for the evaluation of blood plasma from obese patients. The proposed approach was found to provide a statistically sound overview of the biochemical pathways, thus presenting additional information on obesity. Obesity progression was demonstrated to be accompanied by marked alterations in steroidogenesis, androstenedione metabolism, and androgen and estrogen metabolism. The findings of this study suggest that the workflow used for blood analysis is sufficient to demonstrate obesity at the biochemical pathway level as well as to monitor the response to treatment. This workflow is also expected to be suitable for studying other metabolic diseases. View Full-Text
Keywords: metabolomics; obesity; mass spectrometry; blood plasma; metabolite set enrichment analysis; biological context; metabolite identification; putatively annotated metabolites metabolomics; obesity; mass spectrometry; blood plasma; metabolite set enrichment analysis; biological context; metabolite identification; putatively annotated metabolites
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MDPI and ACS Style

Lokhov, P.G.; Balashova, E.E.; Trifonova, O.P.; Maslov, D.L.; Ponomarenko, E.A.; Archakov, A.I. Mass Spectrometry-Based Metabolomics Analysis of Obese Patients’ Blood Plasma. Int. J. Mol. Sci. 2020, 21, 568.

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