Identification of the High Molecular Weight Isoform of Phostensin
Abstract
:1. Introduction
2. Results and Discussion
2.1. Identification of the High Molecular Weight of Phostensin
2.2. Phostensin-α Is not a Degraded Form of Phostensin-β
2.3. Phostensin-β Binds to PP1 and F-Actin
3. Experimental Section
3.1. Materials
3.2. Identification of Phostensin-β
3.3. Cell Culture and Transfection
3.4. Chase the Degradation Process of Phostensin
3.5. Immunoprecipitation
3.6. Immunocytochemistry
4. Conclusions
Acknowledgments
Conflicts of Interest
- Author ContributionsYSL and HLH contributed to method design, experiments and data analysis. WTL contributed to data analysis. THL and HBH contributed to method design, data analysis and manuscript writing.
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Lin, Y.-S.; Huang, H.-L.; Liu, W.-T.; Lin, T.-H.; Huang, H.-B. Identification of the High Molecular Weight Isoform of Phostensin. Int. J. Mol. Sci. 2014, 15, 1068-1079. https://doi.org/10.3390/ijms15011068
Lin Y-S, Huang H-L, Liu W-T, Lin T-H, Huang H-B. Identification of the High Molecular Weight Isoform of Phostensin. International Journal of Molecular Sciences. 2014; 15(1):1068-1079. https://doi.org/10.3390/ijms15011068
Chicago/Turabian StyleLin, Yu-Shan, Hsien-Lu Huang, Wei-Ting Liu, Ta-Hsien Lin, and Hsien-Bin Huang. 2014. "Identification of the High Molecular Weight Isoform of Phostensin" International Journal of Molecular Sciences 15, no. 1: 1068-1079. https://doi.org/10.3390/ijms15011068