Separation and Purification of Sulforaphane from Broccoli by Solid Phase Extraction
Abstract
:1. Introduction
2. Results and Discussion
2.1. Optimization of Chromatographic Conditions
2.2. Optimization of the Hydrolyzation from Glucoraphanin to Sulforaphane
2.3. Optimization of SPE Conditions
2.3.1. Choice of Different SPE Cartridges
2.3.2. Influence of the Washing Solvent and Elution Solvent
2.3.3. Influence of Elution Solvent Volume
2.3.4. Validation of the Proposed Method
3. Experimental Section
3.1. Reagents and Materials
3.2. Chromatographic Conditions
3.3. Sample Clean-up and Preparation by SPE
4. Conclusions
Acknowledgments
References
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pH | Amount of sulforaphane (mg/g) |
---|---|
0 | 0.024 |
2.0 | 0.057 |
3.0 | 0.148 |
4.0 | 0.132 |
6.0 | 0.118 |
Washing solvent | Amount of sulforaphane (mg/g) | ||
---|---|---|---|
Silica cartridge | C18 cartridge | Amino cartridge | |
Water | 0.016 | 0.003 | 0.002 |
Acetonitrile | 0.069 | 0.032 | 0.027 |
Dichloromethane | 0.357 | - | 0.057 |
0.1M Acetic acid | - | 0.062 | - |
Hexane | - | - | 0.006 |
Ethyl acetate | 0 | 0 | - |
Volume of dichloromethane (mL) | Amount of sulforaphane (mg/g) |
---|---|
2 | 0.120 |
3 | 0.214 |
4 | 0.324 |
5 | 0.326 |
6 | 0.327 |
Target compound | Linear range (μg/mL) | r2 | LOD (μg/mL) |
---|---|---|---|
Sulforaphane | 0.05–200 | 0.998 | 0.002 |
Original (μg/mg) | Add amount (μg/mg) | Found amount (μg/mg) | Recovery (%) | RSD | |
---|---|---|---|---|---|
Sulforaphane | 0.513 | 10.0 | 9.59 | 90.8 | 3.54 |
0.513 | 50.0 | 47.10 | 93.2 | 3.17 | |
0.513 | 100.0 | 96.91 | 96.4 | 2.36 |
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Han, D.; Row, K.H. Separation and Purification of Sulforaphane from Broccoli by Solid Phase Extraction. Int. J. Mol. Sci. 2011, 12, 1854-1861. https://doi.org/10.3390/ijms12031854
Han D, Row KH. Separation and Purification of Sulforaphane from Broccoli by Solid Phase Extraction. International Journal of Molecular Sciences. 2011; 12(3):1854-1861. https://doi.org/10.3390/ijms12031854
Chicago/Turabian StyleHan, Dandan, and Kyung Ho Row. 2011. "Separation and Purification of Sulforaphane from Broccoli by Solid Phase Extraction" International Journal of Molecular Sciences 12, no. 3: 1854-1861. https://doi.org/10.3390/ijms12031854