Next Article in Journal
Changes of Constituents and Activity to Apoptosis and Cell Cycle During Fermentation of Tea
Previous Article in Journal
Dietary Sources of Fiber Intake and Its Association with Socio-Economic Factors among Flemish Preschool Children
Open AccessArticle

Separation and Purification of Sulforaphane from Broccoli by Solid Phase Extraction

Department of Chemical Engineering, Inha University, 253 Yonghyun-Dong, Nam-Ku, Incheon 402-751, Korea
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2011, 12(3), 1854-1861; https://doi.org/10.3390/ijms12031854
Received: 10 February 2011 / Revised: 1 March 2011 / Accepted: 7 March 2011 / Published: 10 March 2011
(This article belongs to the Section Biochemistry)
A simple solid-phase extraction (SPE) method for the determination of sulforaphane in broccoli has been developed. The optimal conditions were found to be use of a silica SPE cartridge, and ethyl acetate and dichloromethane as washing and eluting solvents, respectively, which could eliminate interferences originating from the broccoli matrix. The extracts were sufficiently clean to be directly injected into high-performance liquid chromatography (HPLC) for further chromatographic analysis. Good linearity was obtained from 0.05 to 200 μg/mL (r = 0.998) for sulforaphane with the relative standard deviations less than 3.6%. The mean recoveries of sulforaphane from broccoli were more than 90.8% and the detection limit (S/N = 3:1) was 0.02 μg/mL. The SPE method provides a higher yield of sulforaphane from crude extracts compared to conventional liquid-liquid extraction. View Full-Text
Keywords: broccoli; sulforaphane; solid-phase extraction broccoli; sulforaphane; solid-phase extraction
Show Figures

MDPI and ACS Style

Han, D.; Row, K.H. Separation and Purification of Sulforaphane from Broccoli by Solid Phase Extraction. Int. J. Mol. Sci. 2011, 12, 1854-1861. https://doi.org/10.3390/ijms12031854

AMA Style

Han D, Row KH. Separation and Purification of Sulforaphane from Broccoli by Solid Phase Extraction. International Journal of Molecular Sciences. 2011; 12(3):1854-1861. https://doi.org/10.3390/ijms12031854

Chicago/Turabian Style

Han, Dandan; Row, Kyung Ho. 2011. "Separation and Purification of Sulforaphane from Broccoli by Solid Phase Extraction" Int. J. Mol. Sci. 12, no. 3: 1854-1861. https://doi.org/10.3390/ijms12031854

Find Other Styles

Article Access Map by Country/Region

1
Only visits after 24 November 2015 are recorded.
Search more from Scilit
 
Search
Back to TopTop