Analysis of Intestinal Injuries Induced by Ricin in Vitro Using SPR Technology and MS Identification
Abstract
:1. Introduction
2. Experimental Section
2.1. Materials
2.2. Brush Border Membrane Protein (BBMP) preparation [6]
2.3. Preparation of biosensor chips
2.4. Kinetics analysis
2.5. Mass-Spectrum identification
3. Results and Discussion
3.1. Immobilisation of BBMPs on the surface of sensor chip
3.2. Kinetics analysis of BBMP and ricin
3.3. Recovery and identification of interaction components
4. Conclusions
Acknowledgments
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NO. | Cover Percent (%) | MW(D) | PI | Identified Name |
---|---|---|---|---|
1 | 1.27 | 208984.41 | 5.7 | sucrase-isomaltase |
2 | 2.35 | 112982.21 | 5.3 | Na+/K+ -ATPase alpha 1 subunit |
3 | 8.13 | 26134.64 | 4.75 | protease, serine, 1 |
4 | 1.18 | 87436.93 | 5.96 | dipeptidylpeptidase 4 |
5 | 2.82 | 81375.48 | 7.31 | Tripartite motif-containing protein 71 |
6 | 1.24 | 109651.12 | 5.62 | alanyl (membrane) aminopeptidase |
7 | 0.92 | 142634.26 | 4.26 | protocadherin 24 |
8 | 0.95 | 103676.35 | 5.49 | WD repeat domain 22 |
9 | 4.67 | 25110.87 | 9.14 | KDEL (Lys-Asp-Glu-Leu) ER protein retention receptor 3 |
10 | 1.28 | 79500.51 | 5.59 | meprin 1 beta |
11 | 1.57 | 71901.5 | 4.76 | Rab11-FIP4-like |
12 | 0.42 | 242072.22 | 6.54 | BAP28 protein |
13 | 0.32 | 278603.97 | 6.27 | X-linked nuclear protein |
14 | 2.67 | 43129.22 | 5.27 | guanylate binding protein 8 |
15 | 3.13 | 35989.95 | 5.43 | annexin A4 |
16 | 11.65 | 11368.23 | 11.18 | histone 4 protein |
17 | 2.37 | 49225.49 | 5.79 | secretion regulating guanine nucleotide exchange factor |
18 | 0.93 | 105319.96 | 5.6 | similar to neurofilament protein |
19 | 0.3 | 369730.12 | 4.97 | URE-binding protein 1 |
20 | 2.28 | 81229.48 | 9.28 | junctophilin 3 |
21 | 0.25 | 413981.02 | 5.46 | similar to maltase-glucoamylase |
22 | 0.57 | 221497.62 | 5.69 | Myosin cardiac muscle beta chain |
23 | 0.27 | 490969.47 | 6.11 | vacuolar protein sorting 13 D |
24 | 4.41 | 40820.65 | 5.32 | t-complex protein |
25 | 7.20 | 13591.96 | 9.21 | Chemokine-like protein TAFA-5. |
26 | 3.48 | 32599.11 | 5.95 | annexin A2 |
27 | 1.55 | 95321.42 | 5.21 | anaphase promoting complex subunit 2 |
28 | 6.39 | 31393.08 | 9.04 | killer cell lectin-like receptor, subfamily A, member 8 |
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Liu, L.; Gao, H.; Li, J.; Dong, Y.; Liu, N.; Wan, J.; Liu, W.; Sun, Y.; Xu, M. Analysis of Intestinal Injuries Induced by Ricin in Vitro Using SPR Technology and MS Identification. Int. J. Mol. Sci. 2009, 10, 2431-2439. https://doi.org/10.3390/ijms10052431
Liu L, Gao H, Li J, Dong Y, Liu N, Wan J, Liu W, Sun Y, Xu M. Analysis of Intestinal Injuries Induced by Ricin in Vitro Using SPR Technology and MS Identification. International Journal of Molecular Sciences. 2009; 10(5):2431-2439. https://doi.org/10.3390/ijms10052431
Chicago/Turabian StyleLiu, Linna, Hongwei Gao, Jiping Li, Ying Dong, Ning Liu, Jiayu Wan, Wensen Liu, Yucheng Sun, and Ming Xu. 2009. "Analysis of Intestinal Injuries Induced by Ricin in Vitro Using SPR Technology and MS Identification" International Journal of Molecular Sciences 10, no. 5: 2431-2439. https://doi.org/10.3390/ijms10052431
APA StyleLiu, L., Gao, H., Li, J., Dong, Y., Liu, N., Wan, J., Liu, W., Sun, Y., & Xu, M. (2009). Analysis of Intestinal Injuries Induced by Ricin in Vitro Using SPR Technology and MS Identification. International Journal of Molecular Sciences, 10(5), 2431-2439. https://doi.org/10.3390/ijms10052431