Next Article in Journal
Uncovering the Properties of Energy-Weighted Conformation Space Networks with a Hydrophobic-Hydrophilic Model
Next Article in Special Issue
Coenzyme Autocatalytic Network on the Surface of Oil Microspheres as a Model for the Origin of Life
Previous Article in Journal
Phenolic Compounds Protect Cultured Hippocampal Neurons against Ethanol-Withdrawal Induced Oxidative Stress
Previous Article in Special Issue
Pseudo-Replication of [GADV]-Proteins and Origin of Life
Article Menu

Export Article

Open AccessArticle

Non-Enzymatic Template-Directed Recombination of RNAs

Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of Russian Academy of Sciences, 8 Lavrentiev Avenue, Novosibirsk 630090, Russian Federation
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2009, 10(4), 1788-1807;
Received: 31 March 2009 / Revised: 10 April 2009 / Accepted: 15 April 2009 / Published: 21 April 2009
(This article belongs to the Special Issue Origin of Life)
PDF [714 KB, uploaded 19 June 2014]


RNA non-enzymatic recombination reactions are of great interest within the hypothesis of the "RNA world", which argues that at some stage of prebiotic life development proteins were not yet engaged in biochemical reactions and RNA carried out both the information storage task and the full range of catalytic roles necessary in primitive self-replicating systems. Here we report on the study of recombination reaction occuring between two 96 nucleotides (nts) fragments of RNAs under physiological conditions and governed by a short oligodeoxyribonucleotide template, partially complementary to sequences within each of the RNAs. Analysis of recombination products shows that ligation is predominantly template-directed, and occurs within the complementary complex with the template in "butt-to-butt" manner, in 1- or 3- nts bulges or in 2-3 nts internal loops. Minor recombination products formed in the template-independent manner are detected as well. View Full-Text
Keywords: RNA; non-enzymatic recombination; non-enzymatic ligation; RNA bulge loops; RNA internal loops; RNA world; origin of life RNA; non-enzymatic recombination; non-enzymatic ligation; RNA bulge loops; RNA internal loops; RNA world; origin of life

Graphical abstract

This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Share & Cite This Article

MDPI and ACS Style

Nechaev, S.Y.; Lutay, A.V.; Vlassov, V.V.; Zenkova, M.A. Non-Enzymatic Template-Directed Recombination of RNAs. Int. J. Mol. Sci. 2009, 10, 1788-1807.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top