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Endothelium-Independent Vasorelaxant Effect of Ligusticum jeholense Root and Rhizoma on Rat Thoracic Aorta

Department of Herbology, College of Korean Medicine, Kyung Hee University, Seoul 130-701, Korea
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Academic Editor: Derek J. McPhee
Molecules 2015, 20(6), 10721-10733; https://doi.org/10.3390/molecules200610721
Received: 23 April 2015 / Revised: 6 June 2015 / Accepted: 8 June 2015 / Published: 10 June 2015
(This article belongs to the Section Natural Products Chemistry)
Ligusticum jeholense has been used as the traditional medicine ‘Go-Bon’ (Chinese name, Gao-ben) in China and Korea. Considering the increased use of medicinal herbs to treat hypertension, in this study, we aimed to investigate the mechanisms of the vasorelaxation effect caused by L. jeholense. We tested the methanol (MeOH) extract of L. jeholense root and rhizoma for vasorelaxant effects; while using an isolated organ-chamber technique, L. jeholense extract (LJE) induced relaxation in the rat aortic rings by stimulating vascular endothelial and smooth muscle cells. LJE showed concentration-dependent relaxant effects on endothelium-intact and endothelium-denuded aortic rings pre-contracted with both phenylephrine (PE) and potassium chloride (KCl) in Krebs-Henseleit (KH) buffer. The vasorelaxant effect of LJE was partly attenuated by pre-treatment with glibenclamide or 4-aminopyridine (4-AP) as K+ channel blockers. Moreover, LJE showed concentration-dependent inhibition of vasoconstriction by Ca2+ supplementation in the aortic rings that were pre-contracted with PE or KCl in Ca2+-free KH buffer. In addition, a combination of LJE and nifedipine, pre-incubated further, decreased PE-induced contractions. The results suggested that LJE-induced vasorelaxation were related to blocking K+ channels and inhibiting entry of extracellular Ca2+ via receptor-operative Ca2+ channels (ROCCs) or voltage-dependent Ca2+ channels (VDCCs). View Full-Text
Keywords: Ligusticum jeholense; vasorelaxant effect; potassium channels; calcium channels; hypertension Ligusticum jeholense; vasorelaxant effect; potassium channels; calcium channels; hypertension
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Kim, B.; Lee, K.; Chinannai, K.S.; Ham, I.; Bu, Y.; Kim, H.; Choi, H.-Y. Endothelium-Independent Vasorelaxant Effect of Ligusticum jeholense Root and Rhizoma on Rat Thoracic Aorta. Molecules 2015, 20, 10721-10733.

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