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Antioxidant Activity of Leaf Extracts from Different Hibiscus sabdariffa Accessions and Simultaneous Determination Five Major Antioxidant Compounds by LC-Q-TOF-MS

1
SFA Key Laboratory of Bamboo and Rattan Science and Technology, International Centre for Bamboo and Rattan, No. 8 Futong Dongdajie, Wangjing, Chaoyang District, Beijing 100102, China
2
Southern University Agricultural Research and Extension Center, Baton Rouge, LA 70813, USA
3
Urban Forestry Program, College of Science and Agriculture, Southern University, Baton Rouge, LA 70813, USA
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Molecules 2014, 19(12), 21226-21238; https://doi.org/10.3390/molecules191221226
Received: 19 October 2014 / Revised: 27 November 2014 / Accepted: 12 December 2014 / Published: 17 December 2014
(This article belongs to the Section Natural Products Chemistry)
Hibiscus sabdariffa has gained attention for its antioxidant activity. There are many accessions of H. sabdariffa in the world. However, information on the quantification of antioxidant compounds in different accessions is rather limited. In this paper, a liquid chromatography/quadrupole-time-of-flight mass spectrometry (LC-Q-TOF-MS) method for simultaneous determination of five antioxidant compounds (neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, rutin, and isoquercitrin) in H. sabdariffa leaves was developed. The method was validated for linearity, sensitivity, precision, repeatability and accuracy. The validated method has been successfully applied for determination of the five analytes in eight accessions of H. sabdariffa. The eight accessions of H. sabdariffa were evaluated for their antioxidant activities by DPPH free radical scavenging assay. The investigated accessions of H. sabdariffa were rich in rutin and exhibited strong antioxidant activity. The two accessions showing the highest antioxidant activities were from Cuba (No. 2) and Taiwan (No. 5). The results indicated that H. sabdariffa leaves could be considered as a potential antioxidant source for the food industry. The developed LC-Q-TOF-MS method is helpful for quality control of H. sabdariffa. View Full-Text
Keywords: Hibiscus sabdariffa; antioxidant activity; LC-Q-TOF-MS; method validation; DPPH; antioxidant compounds; quantitative analysis Hibiscus sabdariffa; antioxidant activity; LC-Q-TOF-MS; method validation; DPPH; antioxidant compounds; quantitative analysis
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Wang, J.; Cao, X.; Jiang, H.; Qi, Y.; Chin, K.L.; Yue, Y. Antioxidant Activity of Leaf Extracts from Different Hibiscus sabdariffa Accessions and Simultaneous Determination Five Major Antioxidant Compounds by LC-Q-TOF-MS. Molecules 2014, 19, 21226-21238.

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