Chemopreventive Effects of Peucedanum praeruptorum DUNN and Its Major Constituents on SGC7901 Gastric Cancer Cells
1
School of Pharmaceutical Science, Shanxi Medical University, No 56, Xinjian Nan Road, Taiyuan 030001, Shanxi, China
2
School of Public Health, Shanxi Medical University, No 56, Xinjian Nan Road, Taiyuan 030001, Shanxi, China
*
Author to whom correspondence should be addressed.
Molecules 2010, 15(11), 8060-8071; https://doi.org/10.3390/molecules15118060
Received: 6 October 2010
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Revised: 1 November 2010
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Accepted: 3 November 2010
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Published: 9 November 2010
In this study, the effects of Peucedanum praeruptorum DUNN methanolic extract (PPME) and its major constituents on SGC7901 human gastric cancer cells were evaluated. Two pyranocoumarins, namely, (±) praeruptorin A (PA) and (±) praeruptorin B (PB), were isolated from PPME. A high performance liquid chromatographic (HPLC) method was developed to determine the contents of PA and PB in PPME. The antiproliferative and cytotoxic actions of PPME were observed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and release of lactate dehydrogenase (LDH) assays. At 300 µg/mL, PPME inhibited cell growth by 51.2% (P < 0.01), probably linked to the high concentration of PA and PB. Both PA and PB exhibited antiproliferative and cytotoxic activities on the SGC7901 cells. Furthermore, the active principle compound, PA, also enhanced the actions of doxorubincin (DOX) on SGC7901 cells. Cell growth decreased higher with the combined treatment of PA and DOX than that with the chemotherapy agent applied alone, suggesting that PA could reduce the dose of DOX for the desired effects.
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Keywords:
Peucedanum praeruptorum DUNN; (±) praeruptorin A; (±) praeruptorin B; chemopreventive effect; SGC7901 gastric cancer cell
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MDPI and ACS Style
Liang, T.; Yue, W.; Li, Q. Chemopreventive Effects of Peucedanum praeruptorum DUNN and Its Major Constituents on SGC7901 Gastric Cancer Cells. Molecules 2010, 15, 8060-8071. https://doi.org/10.3390/molecules15118060
AMA Style
Liang T, Yue W, Li Q. Chemopreventive Effects of Peucedanum praeruptorum DUNN and Its Major Constituents on SGC7901 Gastric Cancer Cells. Molecules. 2010; 15(11):8060-8071. https://doi.org/10.3390/molecules15118060
Chicago/Turabian StyleLiang, Taigang, Wenyan Yue, and Qingshan Li. 2010. "Chemopreventive Effects of Peucedanum praeruptorum DUNN and Its Major Constituents on SGC7901 Gastric Cancer Cells" Molecules 15, no. 11: 8060-8071. https://doi.org/10.3390/molecules15118060
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