Evaluation on Anti-hepatitis Viral Activity of Vitis vinifer L
Abstract
:1. Introduction
2. Results and Discussion
2.1. Toxic effect of extracts from suosuo grape on Hep G2.2.15 cells
2.2. Inhibitory effect of extracts from suosuo grape on the expression of HBsAg, HBeAg and HBV DNA replication
2.3. Inhibitory effect of extracts from suosuo grape on the expression of HBsAg, HBeAg and HBV DNA replication
3. Experimental
3.1. Plant material
3.2. Extraction and isolation
3.3. Oleanolic acid content of VTT
3.4. Flavonoid content of VTF
3.5. Experiment design
3.6. Cell culture
3.7. Cytotoxicity assays
3.8. HBsAg and HBeAg analysis
3.9. Detection of HBV DNA
3.10. Statistical analysis
4. Conclusions
Acknowledgements
References and Notes
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Sample Availability: Samples are available from the authors. |
Sample | TC50(μg/mL) | HBsAg | HBeAg | ||
---|---|---|---|---|---|
IC50(μg/mL) | TI | IC50(μg/mL) | TI | ||
VTT | 160.61 | 62.54 | 2.57 | 89.28 | 1.80 |
VTP | 1412.85 | 112.69 | 12.54 | 204.46 | 6.91 |
VTF | 284.91 | 327.56 | 0.87 | 215.34 | 1.32 |
3-TC | 244.77 | 315.37 | 0.78 | 9051.01 | 0.03 |
Sample | Concentration(μg/mL) | HBV DNA(copies/mL) | HBV DNA inhibition ratio (%) |
---|---|---|---|
VTT | 50 | (4.842 ± 4.457) × 105 | 59.17 |
25 | (8.269 ± 2.002) × 105 | 30.28 | |
12.5 | (0.944 ± 1.460) × 106 | 20.40 | |
6.25 | (1.015 ± 5.230) × 106 | 14.42 | |
VTP | 100 | (4.823 ± 3.394) × 105 | 59.33 |
50 | (7.525 ± 5.883) × 105 | 36.55 | |
25 | (0.873 ± 5.335) × 106 | 26.39 | |
12.5 | (1.158 ± 2.027) × 106 | 2.36 | |
VTF | 100 | (6.211 ± 1.910) × 105 | 47.63 |
50 | (0.970 ± 0.432) × 106 | 18.21 | |
25 | (0.892 ± 2.872) × 106 | 24.79 | |
12.5 | (1.162 ± 0.201) × 106 | 2.02 | |
3-TC | 100 | < 103 | > 99.99 |
10 | < 103 | > 99.99 | |
1 | < 103 | > 99.99 | |
Cell group | (1.186 ± 0.226)×106 |
NO | A | B | A×B | C | A×C | B×C | A×B×C | HBeAg inhibition ratio(%) | |||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
1 | 2 | 3 | 4 | 5 | 6 | 7 | X1 | X2 | X3 | ΣX | |||
1 | 1(50) | 1(50) | 1 | 1(50) | 1 | 1 | 1 | 65.55 | 60.24 | 45.88 | 171.67 | ||
2 | 1(50) | 1(50) | 1 | 2(25) | 2 | 2 | 2 | 46.29 | 49.39 | 54.45 | 150.13 | ||
3 | 1(50) | 2(25) | 2 | 1(50) | 1 | 2 | 2 | 51.18 | 46.37 | 46.86 | 144.41 | ||
4 | 1(50) | 2(25) | 2 | 2(25) | 2 | 1 | 1 | 38.53 | 29.96 | 41.22 | 109.71 | ||
5 | 2(25) | 1(50) | 2 | 1(50) | 2 | 1 | 2 | 59.84 | 64.33 | 69.06 | 193.23 | ||
6 | 2(25) | 1(50) | 2 | 2(25) | 1 | 2 | 1 | 48.08 | 37.31 | 35.59 | 120.98 | ||
7 | 2(25) | 2(25) | 1 | 1(50) | 2 | 2 | 1 | 46.12 | 48.08 | 29.80 | 124.00 | ||
8 | 2(25) | 2(25) | 1 | 2(25) | 1 | 1 | 2 | 64.08 | 58.94 | 47.58 | 170.60 | ||
Ⅰ | 575.92 | 636.01 | 616.40 | 633.31 | 607.66 | 645.21 | 526.36 | ||||||
Ⅱ | 608.81 | 548.72 | 568.33 | 551.42 | 577.07 | 539.52 | 658.37 | ||||||
R | 32.89 | 87.29 | 48.07 | 81.89 | 30.59 | 105.69 | 132.01 |
The source of variation | SS | υ | MS | F | Sig. |
---|---|---|---|---|---|
Total variation | 2771.173 | 23 | |||
Total inter-treatment | 1968.782 | 7 | |||
A | 45.073 | 1 | 45.073 | 0.899 | 0.357 |
B | 317.481 | 1 | 317.481 | 6.331 | 0.023 |
C | 279.416 | 1 | 279.416 | 5.572 | 0.031 |
Frist interaction A×B | 96.280 | 1 | 96.280 | 1.920 | 0.185 |
A×C | 38.990 | 1 | 38.990 | 0.777 | 0.391 |
B×C | 465.432 | 1 | 465.432 | 9.281 | 0.008 |
Second interaction A×B×C | 726.110 | 1 | 726.110 | 14.479 | 0.002 |
Error | 802.392 | 16 | 50.149 |
Variable | Level | |
---|---|---|
1 | 2 | |
A, VTT | 50 | 25 |
B, VTP | 50 | 25 |
C, VTF | 50 | 25 |
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Liu, T.; Zhao, J.; Li, H.; Ma, L. Evaluation on Anti-hepatitis Viral Activity of Vitis vinifer L. Molecules 2010, 15, 7415-7422. https://doi.org/10.3390/molecules15107415
Liu T, Zhao J, Li H, Ma L. Evaluation on Anti-hepatitis Viral Activity of Vitis vinifer L. Molecules. 2010; 15(10):7415-7422. https://doi.org/10.3390/molecules15107415
Chicago/Turabian StyleLiu, Tao, Jun Zhao, Haibo Li, and Long Ma. 2010. "Evaluation on Anti-hepatitis Viral Activity of Vitis vinifer L" Molecules 15, no. 10: 7415-7422. https://doi.org/10.3390/molecules15107415
APA StyleLiu, T., Zhao, J., Li, H., & Ma, L. (2010). Evaluation on Anti-hepatitis Viral Activity of Vitis vinifer L. Molecules, 15(10), 7415-7422. https://doi.org/10.3390/molecules15107415