MDPI - Publisher of Open Access Journals

25 pages, 2090 KiB

Open AccessArticle

Microcystis aeruginosa msoT1/msoA1 Locus Displays Features of a Type I Toxin–Antitoxin System

by Matija Ruparčič and Marko Dolinar

Toxins 2025, 17(8), 360; https://doi.org/10.3390/toxins17080360 (registering DOI) - 22 Jul 2025

Type I toxin–antitoxin (TA) systems consist of a protein toxin that exerts a cytostatic or cytotoxic effect and an antisense RNA antitoxin that prevents translation of the toxin. Although well studied, type I TA systems have so far only been discovered in bacteria [...] Read more.

Type I toxin–antitoxin (TA) systems consist of a protein toxin that exerts a cytostatic or cytotoxic effect and an antisense RNA antitoxin that prevents translation of the toxin. Although well studied, type I TA systems have so far only been discovered in bacteria from the phyla Proteobacteria, Firmicutes, and Tenericutes. We hypothesized that type I systems could also be present in Cyanobacteria. Through bioinformatic analysis of the Microcystis aeruginosa PCC 7806SL genome, we discovered ten putative type I TA loci and characterized six of them experimentally. Two of the six putative type I toxins, BH695_0320 and MsoT1 (BH695_4017), were observed to negatively affect Escherichia coli cell growth, with MsoT1 exerting a phenotype similar to SrnB, a known type I toxin. We focused on the MsoT1/MsoA1 TA system and confirmed the expression of MsoT1 and MsoA1 in our assay. Additionally, we found that MsoA1 delays the toxic effects of MsoT1, indicating its role as a cognate antitoxin of MsoT1. Our results suggest that MsoT1/MsoA1 represents a novel candidate type I TA system, the first to be discovered in the Cyanobacteria phylum. Full article

(This article belongs to the Section Bacterial Toxins)

► Show Figures

Figure 1

18 pages, 2502 KiB

Open AccessArticle

Learning Local Texture and Global Frequency Clues for Face Forgery Detection

by Xin Jin, Yuru Kou, Yuhao Xie, Yuying Zhao, Miss Laiha Mat Kiah, Qian Jiang and Wei Zhou

Biomimetics 2025, 10(8), 480; https://doi.org/10.3390/biomimetics10080480 (registering DOI) - 22 Jul 2025

Abstract

In recent years, the rapid advancement of deep learning techniques has significantly propelled the development of face forgery methods, drawing considerable attention to face forgery detection. However, existing detection methods still struggle with generalization across different datasets and forgery techniques. In this work, [...] Read more.

In recent years, the rapid advancement of deep learning techniques has significantly propelled the development of face forgery methods, drawing considerable attention to face forgery detection. However, existing detection methods still struggle with generalization across different datasets and forgery techniques. In this work, we address this challenge by leveraging both local texture cues and global frequency domain information in a complementary manner to enhance the robustness of face forgery detection. Specifically, we introduce a local texture mining and enhancement module. The input image is segmented into patches and a subset is strategically masked, then texture enhanced. This joint masking and enhancement strategy forces the model to focus on generalizable localized texture traces, mitigates overfitting to specific identity features and enabling the model to capture more meaningful subtle traces of forgery. Additionally, we extract multi-scale frequency domain features from the face image using wavelet transform, thereby preserving various frequency domain characteristics of the image. And we propose an innovative frequency-domain processing strategy to adjust the contributions of different frequency-domain components through frequency-domain selection and dynamic weighting. This Facilitates the model’s ability to uncover frequency-domain inconsistencies across various global frequency layers. Furthermore, we propose an integrated framework that combines these two feature modalities, enhanced with spatial attention and channel attention mechanisms, to foster a synergistic effect. Extensive experiments conducted on several benchmark datasets demonstrate that the proposed technique demonstrates superior performance and generalization capabilities compared to existing methods. Full article

(This article belongs to the Special Issue Exploration of Bioinspired Computer Vision and Pattern Recognition)

► Show Figures

Figure 1

41 pages, 1710 KiB

Open AccessArticle

Toward Integrated Satellite Operations and Network Management: A Review and Novel Framework

by Arnau Singla, Franco Criscola, David Canales, Juan A. Fraire, Anna Calveras and Joan A. Ruiz-de-Azua

Technologies 2025, 13(8), 312; https://doi.org/10.3390/technologies13080312 (registering DOI) - 22 Jul 2025

Abstract

Achieving global coverage and performance goals for 6G requires seamless integration of satellite and terrestrial networks, yet current operational frameworks lack common standards for managing these heterogeneous infrastructures. This paper addresses the critical need for unified satellite-terrestrial network operations by proposing the CMS [...] Read more.

Achieving global coverage and performance goals for 6G requires seamless integration of satellite and terrestrial networks, yet current operational frameworks lack common standards for managing these heterogeneous infrastructures. This paper addresses the critical need for unified satellite-terrestrial network operations by proposing the CMS framework, a novel task-scheduling-based approach that bridges the operational gap between satellite operations (SatOps) and network operations (NetOps). The framework integrates satellite-specific constraints with network service requirements and QoS metrics through constraint satisfaction programming and multi-objective optimization. Three novel architectures are introduced: integrated operations (embedding NetOps within SatOps), coordinated operations (unified control with separate execution channels), and adaptive operations (mutual adaptation through intelligent interfaces). Each architecture addresses different connectivity scenarios and integration requirements for both sporadic and persistent satellite constellations. The proposed architectures are evaluated against challenges spanning infrastructure and architecture, interoperability and standardization, integrated management, operational dynamics, and technology maturation and deployment. Validation through simulation demonstrates significant performance improvements, with task completion rates improving by 17.87% to 44.02% and data throughput gains of 25.09% to 93.62% compared to traditional approaches. The CMS framework establishes a resilient operational standard for future 6G networks, offering practical solutions to bridge the current divide between satellite and terrestrial network operations. Full article

(This article belongs to the Section Information and Communication Technologies)

► Show Figures

Figure 1

18 pages, 6673 KiB

Open AccessArticle

Tribological Properties of MoN/TiN Multilayer Coatings Prepared via High-Power Impulse Magnetron Sputtering

by Jiaming Xu, Ping Zhang, Jianjian Yu, Puyou Ying, Tao Yang, Jianbo Wu, Tianle Wang, Nikolai Myshkin and Vladimir Levchenko

Lubricants 2025, 13(8), 319; https://doi.org/10.3390/lubricants13080319 (registering DOI) - 22 Jul 2025

Abstract

To address the limitations of single-layer nitride coatings, such as poor load adaptability and low long-term durability, MoN/TiN multilayer coatings were prepared via high-power impulse magnetron sputtering (HiPIMS). HiPIMS produces highly ionized plasmas that enable intense ion bombardment, yielding nitride films with enhanced [...] Read more.

To address the limitations of single-layer nitride coatings, such as poor load adaptability and low long-term durability, MoN/TiN multilayer coatings were prepared via high-power impulse magnetron sputtering (HiPIMS). HiPIMS produces highly ionized plasmas that enable intense ion bombardment, yielding nitride films with enhanced mechanical strength, durability, and thermal stability versus conventional methods. The multilayer coating demonstrated a low coefficient of friction (COF, ~0.4) and wear rate (1.31 × 10⁻⁷ mm³/[N·m]). In contrast, both TiN and MoN coatings failed at 5 N and 10 N loads, respectively. Under increasing loads, the multilayer coating maintained stable wear rates (1.84–3.06 × 10⁻⁷ mm³/[N·m]) below 20 N, and ultimately failed at 25 N. Furthermore, the MoN layer contributes to COF reduction. Grazing-incidence X-ray diffraction analysis confirmed the enhanced crystallographic stability of the multilayer coating, thereby revealing a dominant (111) orientation. The multilayer architecture suppresses crack propagation while effectively balancing hardness and toughness, offering a promising design for extreme-load applications. Full article

► Show Figures

Figure 1

15 pages, 1443 KiB

Open AccessArticle

Prediction of Waiting Lists for Medical Specialties in Hospitals in Costa Rica Using Queuing Theory and Monte Carlo Simulation

by Bernal Vargas-Vargas, Erick Pérez-Murillo, Jaime González-Domínguez and Justo García-Sanz-Calcedo

Hospitals 2025, 2(3), 17; https://doi.org/10.3390/hospitals2030017 (registering DOI) - 22 Jul 2025

Abstract

This study applies stochastic discrete event modeling to demonstrate that reducing wait times for specialized outpatient clinics in the Costa Rican public healthcare system is possible. The classification process identified four medical specialties with the longest wait times. It includes the creation of [...] Read more.

This study applies stochastic discrete event modeling to demonstrate that reducing wait times for specialized outpatient clinics in the Costa Rican public healthcare system is possible. The classification process identified four medical specialties with the longest wait times. It includes the creation of a separate queuing theory model for each specialty. The birth and death model allowed for estimating the number of arrivals and consultations in the simulation. Validation was performed by comparing the model’s input and output data with real-world statistical reports. An analysis of medical specialists revealed that approximately 22% of patients referred to secondary care did not require specialized medical consultation. Through simulation and the use of stochastic input data, patient waiting times decreased. In an optimistic scenario, waiting times decreased steadily across all specialties over 24 months. Ophthalmology and orthopedics reduced their waiting times to less than 300 days. Otorhinolaryngology decreased from 370 to 250 days, and urology showed the most significant improvement, decreasing from 350 to 100 days in the first year and remaining stable. This evidence transforms the traditional paradigm of increasing capacity as the only solution to the waiting list problem and positions improving the referral process as an alternative. To achieve these results, the study highlights the importance of implementing improved triage protocols in primary care, integrating decision-support tools for general practitioners using machine learning, for example, to reduce unnecessary referrals. Training programs and feedback mechanisms could also align referral practices with specialty criteria. While these strategies were not implemented in this study, the simulation results provide a solid basis for their design and future evaluation. Full article

► Show Figures

Figure 1

28 pages, 2072 KiB

Open AccessReview

Advances in Epstein–Barr Virus Detection: From Traditional Methods to Modern Technologies

by Yidan Sun, Shuyu Ling, Dani Tang, Meimei Yang and Chao Shen

Viruses 2025, 17(8), 1026; https://doi.org/10.3390/v17081026 (registering DOI) - 22 Jul 2025

Abstract

The Epstein–Barr virus (EBV) is a prevalent virus linked to various diseases, including infectious mononucleosis (IM), nasopharyngeal carcinoma, and Hodgkin’s lymphoma. Over the past few decades, EBV diagnostic strategies have evolved significantly—progressing from traditional serological assays and histopathology to more sensitive and specific [...] Read more.

The Epstein–Barr virus (EBV) is a prevalent virus linked to various diseases, including infectious mononucleosis (IM), nasopharyngeal carcinoma, and Hodgkin’s lymphoma. Over the past few decades, EBV diagnostic strategies have evolved significantly—progressing from traditional serological assays and histopathology to more sensitive and specific molecular techniques such as nucleic acid amplification and high-throughput sequencing (HTS). While conventional methods remain valuable for their accessibility and established clinical use, they are often limited by sensitivity, speed, and multiplexing capability. In contrast, emerging technologies, including isothermal amplification, Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-based diagnostics, multi-omics integration, and AI-assisted analysis, have demonstrated great promise in improving diagnostic accuracy, speed, and applicability in diverse clinical settings, including point-of-care testing (POCT). This review systematically explores the historical development of EBV diagnostic technologies, highlighting key milestones and future trends in precision medicine and global health readiness. Full article

(This article belongs to the Special Issue EBV and Disease: New Perspectives in the Post COVID-19 Era)

► Show Figures

Figure 1

13 pages, 579 KiB

Open AccessArticle

Molecular Epidemiology of Beak and Feather Disease Virus (BFDV), Avian Polyomavirus (APV-1), Psittacid Herpesvirus 1 (PsHV-1), and Avian Metapneumovirus (aMPV) in Birds Kept as Non-Traditional Companion Animals (NTCAs) in Italy

by Riccardo Baston, Claudia Maria Tucciarone, Alberto Caudullo, Francesca Poletto, Matteo Legnardi, Mattia Cecchinato, Michele Drigo, Giovanni Franzo and Diego Cattarossi

Animals 2025, 15(15), 2164; https://doi.org/10.3390/ani15152164 (registering DOI) - 22 Jul 2025

Abstract

The non-traditional companion animal (NTCA) sector, particularly involving avian species, has significantly expanded in Italy, raising concerns over the spread of infectious diseases. These animals can harbor various pathogens and act as reservoirs, posing risks to native wildlife through legal or illegal trade, [...] Read more.

The non-traditional companion animal (NTCA) sector, particularly involving avian species, has significantly expanded in Italy, raising concerns over the spread of infectious diseases. These animals can harbor various pathogens and act as reservoirs, posing risks to native wildlife through legal or illegal trade, escapes, or intentional releases. However, the epidemiology of avian pathogens in NTCAs remains poorly understood and is typically investigated only in symptomatic individuals. In the present study, cloacal and choanal cleft swabs were collected from 319 ornamental and raptor birds across 19 families, pooled and tested for beak and feather disease virus (BFDV), avian polyomavirus (APV-1), psittacid herpesvirus 1 (PsHV-1), and avian metapneumovirus (aMPV). BFDV and APV-1 were detected in 13.79% and 2.19% of birds, respectively, with five co-infections. No cases of PsHV-1 or aMPV were found. Both viruses showed a higher prevalence than in previous Italian and most of international studies, with several non-psittacine species, including birds of prey, testing positive—some for the first time. Mixed-species settings and participation in public exhibitions were proven as significant infection risk factors. The study highlights the growing relevance of BFDV and APV-1 in non-commercial birds and recommends improved biosecurity and preventive screening to reduce disease spread and safeguard animal health. Full article

(This article belongs to the Special Issue Exotic Animal Medicine and Surgery—Recent Advances and Perspectives)

► Show Figures

Figure 1

26 pages, 1310 KiB

Open AccessReview

Combination Strategies with HSP90 Inhibitors in Cancer Therapy: Mechanisms, Challenges, and Future Perspectives

by Yeongbeom Kim, Su Yeon Lim, Hyun-Ouk Kim, Suk-Jin Ha, Jeong-Ann Park, Young-Wook Won, Sehyun Chae and Kwang Suk Lim

Pharmaceuticals 2025, 18(8), 1083; https://doi.org/10.3390/ph18081083 (registering DOI) - 22 Jul 2025

Abstract

Heat shock protein 90 (HSP90) is a molecular chaperone that plays a pivotal role in the stabilization and functional activation of numerous oncoproteins and signaling molecules essential for cancer cell survival and proliferation. Despite the extensive development and clinical evaluation of HSP90 inhibitors, [...] Read more.

Heat shock protein 90 (HSP90) is a molecular chaperone that plays a pivotal role in the stabilization and functional activation of numerous oncoproteins and signaling molecules essential for cancer cell survival and proliferation. Despite the extensive development and clinical evaluation of HSP90 inhibitors, their therapeutic potential as monotherapies has been limited by suboptimal efficacy, dose-limiting toxicity, and the emergence of drug resistance. Recent studies have demonstrated that combination therapies involving HSP90 inhibitors and other anticancer agents such as chemotherapeutics, targeted therapies, and immune checkpoint inhibitors can enhance anticancer activity, overcome resistance mechanisms, and modulate the tumor microenvironment. These synergistic effects are mediated by the concurrent degradation of client proteins, the disruption of signaling pathways, and the enhancement of antitumor immunity. However, the successful clinical implementation of such combination strategies requires the careful optimization of dosage, administration schedules, toxicity management, and patient selection based on predictive biomarkers. In this review, we provide a comprehensive overview of the mechanistic rationale, preclinical and clinical evidence, and therapeutic challenges associated with HSP90 inhibitor-based combination therapies. We also discuss future directions leveraging emerging technologies including multi-omics profiling, artificial intelligence, and nanoparticle-mediated delivery for the development of personalized and effective combination regimens in oncology. Full article

(This article belongs to the Special Issue Hsp90 Inhibitors: Progress, Challenges, and Opportunities in Drug Discovery and Development)

► Show Figures

Figure 1

12 pages, 3204 KiB

Open AccessSystematic Review

Association Between ABO or Rh Blood Groups and Chikungunya Virus Infection: A Systematic Review and Meta-Analysis

by Yanisa Rattanapan, Wanatsanan Chulrik, Karunaithas Rasaratnam and Thitinat Duangchan

Medicina 2025, 61(8), 1316; https://doi.org/10.3390/medicina61081316 (registering DOI) - 22 Jul 2025

Abstract

Background and Objectives: The relationship between ABO or Rh blood groups and susceptibility to Chikungunya virus (CHIKV) infection remains unclear. This systematic review and meta-analysis aimed to synthesize available evidence on this association. Materials and Methods: Studies reporting ABO and/or Rh [...] Read more.

Background and Objectives: The relationship between ABO or Rh blood groups and susceptibility to Chikungunya virus (CHIKV) infection remains unclear. This systematic review and meta-analysis aimed to synthesize available evidence on this association. Materials and Methods: Studies reporting ABO and/or Rh blood groups and CHIKV infection were searched through PubMed, Scopus, EMBASE, MEDLINE, Ovid, ProQuest, and Google Scholar up to 8 July 2025. A random-effects meta-analysis was conducted to calculate pooled odds ratios (Ors) with 95% CIs. Heterogeneity was assessed using I² statistics. Subgroup analyses were performed based on study design and study quality. Sensitivity analysis was conducted using a leave-one-out method. Publication bias was evaluated via funnel plots and Egger’s test. Results: Seven studies, including 24,828 participants, were included. No significant associations were observed between blood groups A, B, AB, or Rh(D) and CHIKV infection. However, blood group O was significantly associated with an increased risk of CHIKV infection (OR: 1.52, 95% CI: 1.01–2.29, p = 0.043, I² = 95.38%) compared to non-O blood groups. Subgroup analyses showed stable results. Nevertheless, the sensitivity analysis indicated that certain studies had a greater influence on the overall results. In addition, significant publication bias was also detected. Conclusions: Current evidence indicates that blood group O is significantly associated with an increased susceptibility to CHIKV infection. In contrast, no consistent associations were observed for other ABO or Rh blood groups. Due to substantial heterogeneity and methodological limitations, these findings should be interpreted with caution. Further well-designed, large-scale studies with standardized diagnostics are needed to clarify these associations and underlying mechanisms. Full article

(This article belongs to the Section Infectious Disease)

► Show Figures

Figure 1

11 pages, 778 KiB

Open AccessArticle

Gut and Other Differences Between Female and Male Veterans—Vive La Différence? Bringing It All Together

by Martin Tobi, Donald Bradley, Fadi Antaki, MaryAnn Rambus, Noreen Rossi, James Hatfield, Suzanne Fligiel and Benita McVicker

Gastrointest. Disord. 2025, 7(3), 48; https://doi.org/10.3390/gidisord7030048 (registering DOI) - 22 Jul 2025

Abstract

Background: The number of women veterans has been rising steadily since the Gulf War and many assume the functions of their male counterparts. Women face unique obstacles in their service, and it is imperative that differences in physiology not be overlooked so [...] Read more.

Background: The number of women veterans has been rising steadily since the Gulf War and many assume the functions of their male counterparts. Women face unique obstacles in their service, and it is imperative that differences in physiology not be overlooked so as to provide better and appropriate care to our women in uniform. Despite this influx and incorporation of female talent, dedicated reports contrasting female and male veterans are rare, outside of specific psychological studies. We therefore attempt to contrast gut constituents, absorption, innate immune system, and nutritional differences to provide a comprehensive account of similarities and differences between female and male veterans, from our single-center perspective, as this has not been carried out previously. Herein, we obtained a detailed roster of commonly used biomedical tests and some novel entities to detect differences between female and male veterans. The objective of this study was to detect differences in the innate immune system and other ancillary test results to seek differences that may impact the health of female and male veterans differently. Methods: To contrast biochemical and sociomedical parameters in female and male veterans, we studied the data collected on 450 female veterans and contrasted them to a group of approximately 1642 males, sequentially from 1995 to 2022, all selected because of above-average risk for CRC. As part of this colorectal cancer (CRC) screening cross-sectional and longitudinal study, we also collected stool, urine, saliva, and serum specimens. We used ELISA testing to detect stool p87 shedding by the Adnab-9 monoclonal and urinary organ-specific antigen using the BAC18.1 monoclonal. We used the FERAD ratio (blood ferritin/fecal p87), a measure of the innate immune system to gauge the activity of the innate immune system (InImS) by dividing the denominator p87 (10% N-linked glycoprotein detected by ELISA) into the ferritin level (the enumerator, a common lab test to assess anemia). FERAD ratios have not been performed elsewhere despite past Adnab-9 commercial availability so we have had to auto-cite our published data where appropriate. Results: Many differences between female and males were detected. The most impressive differences were those of the InImS where males clearly had the higher numbers (54,957 ± 120,095) in contrast to a much lower level in females (28,621 ± 66,869), which was highly significantly different (p < 0.004). Mortality was higher in males than females (49.4% vs. 24.1%; OR 3.08 [2.40–3.94]; p < 0.0001). Stool p87, which is secreted by Paneth cells and may have a protective function, was lower in males (0.044 ± 0.083) but higher in females (0.063 ± 0.116; p < 0.031). Immunohistochemistry of the Paneth cell-fixed p87 antigen was also higher in females (in the descending colon and rectum). In contrast, male ferritin levels were significantly higher (206.3 ± 255.9 vs. 141.1 ± 211.00 ng/mL; p < 0.0006). Females were less likely to be diabetic (29.4 vs. 37.3%; OR 0.7 [0.55–0.90]; p < 0.006). Females were also more likely to use NSAIDs (14.7 vs. 10.7%, OR 1.08 [1.08–2.00]; p < 0.015). Females also had borderline less GI bleeding by fecal immune tests (FITs), with 13.2% as opposed to 18.2% in males (OR 0.68 [0.46–1.01]; p = 0.057), but were less inclined to have available flexible sigmoidoscopy (OR 0.68 [0.53–0.89]; p < 0.004). Females also had more GI symptomatology, a higher rate of smoking, and were significantly younger than their male counterparts. Conclusions: This study shows significant differences with multiple parameters in female and male veterans Full article

► Show Figures

Figure 1

14 pages, 688 KiB

Open AccessArticle

A Post-Hoc Analysis of Depressive Disorders in Patients with Type 2 Diabetes

by Yegan Pillay, W. Guyton Hornsby, Jr., Chandan K. Saha, Jay Shubrook, Kent A. Crick, Ziyi Yang, Kieren Mather and Mary de Groot

Healthcare 2025, 13(15), 1773; https://doi.org/10.3390/healthcare13151773 (registering DOI) - 22 Jul 2025

Abstract

Background/Objectives: This study is an investigation of the occurrence, remission and recurrence of major depressive disorders (MDDs) in adults with type 2 diabetes (T2DM). Methods: Interviews were conducted with individuals (N = 176) who met the criteria for MDD using the Structured Clinical [...] Read more.

Background/Objectives: This study is an investigation of the occurrence, remission and recurrence of major depressive disorders (MDDs) in adults with type 2 diabetes (T2DM). Methods: Interviews were conducted with individuals (N = 176) who met the criteria for MDD using the Structured Clinical Interview for the DSM-IV-TR (SCID). Results: N = 176 T2DM adults, with a mean (SD) age of 55.5 (10.4) years, 74% of whom were female and 62% were white, completed the Structured Clinical Interview for the DSM-IV-TR (SCID). A mean (SD) number of 1.8 (0.9) episodes of major depression (MDD) were recorded from birth to the date of interview, with a mean (SD) onset age of the first episode of 40.4 (15.9) years. Median (IQR) MDD episode duration was 13.9 (5.6–31.9) months and the median (IQR) cumulative lifetime exposure duration to MDD was 33 (12.9–63.1) months. Kaplan–Meier survival analysis along with the frailty model, to account for the correlation among multiple recurrences or remissions within a subject, indicated that the median first episode duration was shorter than the median second episode duration (14 vs. 37.9 months, p < 0.0001). Of those who had at least three episodes, the median second episode duration was shorter than the median third-episode duration (13.0 vs. 28.0 months, p = 0.006). The median recurrence time following first remission was significantly longer than the median recurrence time following second remission (138.0 vs. 80.6 months, p = 0.02). Conclusions: These results document that clinical depression is recurrent in adults with T2DM. Moreover, depressive episodes in individuals with T2DM are persistent well beyond episode durations observed in the general population. Full article

(This article belongs to the Special Issue Psychodiabetology: The Psycho-Social Challenges of Diabetes)

► Show Figures

Figure 1

24 pages, 11674 KiB

Open AccessArticle

DNAJ Homolog Subfamily C Member 11 Stabilizes SARS-CoV-2 NSP3 to Promote Double-Membrane Vesicle Formation

by Shuying Chen, Shanrong Yang, Xiaoning Li, Junqi Xiang, Jiangyu Cai, Yaokai Wang, Qingqing Li, Na Zang, Jiaxu Wang, Jian Shang and Yushun Wan

Viruses 2025, 17(8), 1025; https://doi.org/10.3390/v17081025 (registering DOI) - 22 Jul 2025

Abstract

Coronaviruses, particularly those classified as highly pathogenic species, pose a significant threat to global health. These viruses hijack host cellular membranes and proteins to facilitate their replication, primarily through the formation of replication organelles (ROs). However, the precise regulatory mechanisms underlying RO formation [...] Read more.

Coronaviruses, particularly those classified as highly pathogenic species, pose a significant threat to global health. These viruses hijack host cellular membranes and proteins to facilitate their replication, primarily through the formation of replication organelles (ROs). However, the precise regulatory mechanisms underlying RO formation remain poorly understood. To elucidate these mechanisms, we conducted mass spectrometry analyses, identifying interactions between the host protein DnaJ homolog subfamily C member 11 (DNAJC11) and the SARS-CoV-2 nonstructural protein 3 (NSP3) protein. Notably, results showed that DNAJC11 depletion reduces SARS-CoV-2 infection, indicating possible positive regulatory involvement. But the ectopic expression of DNAJC11 did not lead to marked alterations in immune or inflammatory responses. DNAJC11 enhanced NSP3 expression stability through endogenous apoptosis pathways and facilitated its interaction with NSP4, thereby promoting the formation of double-membrane vesicles (DMVs). Knockdown of DNAJC11 reduced DMV number and size, accompanied by dysregulation of the endoplasmic reticulum and mitochondria. However, supplementation with DNAJC11 restored both DMV number and size. These findings provide novel insights into the role of DNAJC11 as a host factor that modulates DMV formation and supports SARS-CoV-2 replication by targeting the NSP3 protein. This study advances our understanding of the molecular interactions between host and viral components and highlights DNAJC11 as a potential target for antiviral interventions. Full article

(This article belongs to the Section Coronaviruses)

► Show Figures

Figure 1

40 pages, 2830 KiB

Open AccessReview

Metal Complexes with Hydroxyflavones: A Study of Anticancer and Antimicrobial Activities

by Ljiljana E. Mihajlović, Monica Trif and Marijana B. Živković

Inorganics 2025, 13(8), 250; https://doi.org/10.3390/inorganics13080250 (registering DOI) - 22 Jul 2025

Abstract

Metal chelation to bioactive small molecules is a well-established strategy to enhance the biological activity of the resulting complexes. Among the widely explored structural motifs, the combination of prominent metal centers with naturally inspired derivatives has attracted considerable attention. One such promising platform [...] Read more.

Metal chelation to bioactive small molecules is a well-established strategy to enhance the biological activity of the resulting complexes. Among the widely explored structural motifs, the combination of prominent metal centers with naturally inspired derivatives has attracted considerable attention. One such promising platform is the flavone scaffold, derived from flavonoids and studied since ancient times. Flavones are plant-derived compounds known for their diverse biological activities and health benefits. They exhibit significant structural variability, primarily through backbone modifications such as hydroxylation. Importantly, coordination of metal ions to hydroxylated flavone cores often improves their natural bioactivities, including anticancer and antimicrobial effects. In this review, we summarize transition metal complexes incorporating hydroxyflavone (OH–F) ligands reported over the past 15 years. We provide a concise overview of synthetic approaches and structural characterization, with a particular emphasis on coordination modes (e.g., maltol-type, acetylacetonate-type, catechol-type, and others). Furthermore, we discuss biological evaluation results, especially anticancer and antimicrobial studies, to highlight the therapeutic potential of these complexes. Finally, we suggest directions for the future development of metal-based agents bearing hydroxyflavone moieties through several critical points in terms of the accuracy, reproducibility, and relevance of biological studies involving metal-based compounds. Full article

(This article belongs to the Special Issue Metal Complexes Containing Bioactive Ligands: Structure and Biological Evaluation)

► Show Figures

Graphical abstract

17 pages, 3444 KiB

Open AccessArticle

Multiphysics-Coupled Simulation of Ultrasound-Assisted Tailing Slurry Sedimentation

by Liang Peng and Congcong Zhao

Materials 2025, 18(15), 3430; https://doi.org/10.3390/ma18153430 (registering DOI) - 22 Jul 2025

Abstract

This study establishes a multiphysics coupling model of acoustics, mechanics, and electrostatics through COMSOL, systematically explores the sound field distribution and stress–strain characteristics of tailing particles in sand silos under different frequencies of ultrasonic radiation, and proposes an optimization scheme for the sound [...] Read more.

This study establishes a multiphysics coupling model of acoustics, mechanics, and electrostatics through COMSOL, systematically explores the sound field distribution and stress–strain characteristics of tailing particles in sand silos under different frequencies of ultrasonic radiation, and proposes an optimization scheme for the sound field. The simulation results show that under 28 kHz ultrasonic radiation, the amplitude of sound pressure in the sand silo (173 Pa) is much lower than that at 40 kHz (1220 Pa), which can avoid damaging the original settlement mode of the tail mortar. At the same time, the periodic fluctuation amplitude of its longitudinal sound pressure is significantly greater than 25 kHz, which can promote settlement by enhancing particle tensile and compressive stress, achieving the best comprehensive effect. The staggered placement scheme of the transducer eliminates upward disturbance in the flow field by changing the longitudinal opposing sound field to oblique propagation, reduces energy dissipation, and increases the highest sound pressure level in the compartment to 130 dB. The sound pressure distribution density is significantly improved, further enhancing the settling effect. This study clarifies the correlation mechanism between ultrasound parameters and tailings’ settling efficiency, providing a theoretical basis for parameter optimization of ultrasound-assisted tailing treatment technology. Its results have important application value in the optimization of tailings settling in metal mine tailing filling. Full article

(This article belongs to the Special Issue Resource Recovery in Building Materials: Developing Eco-Friendly Driven Sustainable Binders or Aggregates from Solid Waste)

► Show Figures

Figure 1

24 pages, 6601 KiB

Open AccessArticle

Micromechanical Finite Element Model Investigation of Cracking Behavior and Construction-Related Deficiencies in Asphalt Mixtures

by Liu Yang, Suwei Hou and Haibo Yu

Materials 2025, 18(15), 3426; https://doi.org/10.3390/ma18153426 (registering DOI) - 22 Jul 2025

Abstract

This study investigated the fracture behavior of asphalt mixtures under indirect tensile loading by comparing the performance of homogenized and micromechanical finite element (FEMs) models based on the cohesive zone model (CZM). Five asphalt mixture types were tested experimentally, and both models were [...] Read more.

This study investigated the fracture behavior of asphalt mixtures under indirect tensile loading by comparing the performance of homogenized and micromechanical finite element (FEMs) models based on the cohesive zone model (CZM). Five asphalt mixture types were tested experimentally, and both models were calibrated and validated using load–displacement curves from indirect tensile tests (IDTs). The micromechanical model, incorporating random aggregate generation and three-phase material definition, exhibited significantly higher predictive accuracy (R² = 0.86–0.98) than the homogenized model (R² = 0.66–0.77). The validated micromechanical model was further applied to quantify the impact of construction-related deficiencies—namely, increased air voids, non-continuous gradation, and aggregate segregation. The simulation results showed that higher void content (from 4% to 10%) reduced peak load by up to 35% and increased localized stress concentrations by up to 40%. Discontinuous gradation and uneven aggregate distribution also led to premature crack initiation and more complex fracture paths. These findings demonstrated the value of micromechanical modeling for evaluating sensitivity to mix design and compaction quality, providing a foundation for performance-based asphalt mixture optimization and durability improvement. Full article

(This article belongs to the Section Construction and Building Materials)

► Show Figures

Figure 1

32 pages, 32586 KiB

Open AccessArticle

Magmatic Evolution at the Saindak Cu-Au Deposit: Implications for the Formation of Giant Porphyry Deposits

by Jun Hong, Yasir Shaheen Khalil, Asad Ali Narejo, Xiaoyong Yang, Tahseenullah Khan, Zhihua Wang, Huan Tang, Haidi Zhang, Bo Yang and Wenyuan Li

Minerals 2025, 15(8), 768; https://doi.org/10.3390/min15080768 (registering DOI) - 22 Jul 2025

Abstract

The Chagai porphyry copper belt is a major component of the Tethyan metallogenic domain, which spans approximately 300 km and hosts several giant porphyry copper deposits. The tectonic setting, whether subduction-related or post-collisional, and the deep dynamic processes governing the formation of these [...] Read more.

The Chagai porphyry copper belt is a major component of the Tethyan metallogenic domain, which spans approximately 300 km and hosts several giant porphyry copper deposits. The tectonic setting, whether subduction-related or post-collisional, and the deep dynamic processes governing the formation of these giant deposits remain poorly understood. Mafic microgranular enclaves (MMEs), mafic dikes, and multiple porphyries have been documented in the Saindak mining area. This work examines both the ore-rich and non-ore intrusions in the Saindak porphyry Cu-Au deposit, using methods like molybdenite Re-Os dating, U-Pb zircon ages, Hf isotopes, and bulk-rock geochemical data. Geochronological results indicate that ore-fertile and barren porphyries yield ages of 22.15 ± 0.22 Ma and 22.21 ± 0.33 Ma, respectively. Both MMEs and mafic dikes have zircons with nearly identical ²⁰⁶Pb/²³⁸U weighted mean ages (21.21 ± 0.18 Ma and 21.21 ± 0.16 Ma, respectively), corresponding to the age of the host rock. Geochemical and Sr–Nd–Hf isotopic evidence indicates that the Saindak adakites were generated by the subduction of the Arabian oceanic lithosphere under the Eurasian plate, rather than through continental collision. The adakites were mainly formed by the partial melting of a metasomatized mantle wedge, induced by fluids from the dehydrating subducting slab, with minor input from subducted sediments and later crust–mantle interactions during magma ascent. We conclude that shallow subduction of the Arabian plate during the Oligocene–Miocene may have increased the flow of subducted fluids into the sub-arc mantle source of the Chagai arc. This process may have facilitated the widespread deposition of porphyry copper and copper–gold mineralization in the region. Full article

(This article belongs to the Section Mineral Deposits)

► Show Figures

Figure 1

20 pages, 10834 KiB

Open AccessArticle

Genesis of Basalts of the Raohe Subduction–Accretion Complex in the Wandashan Block, NE China, and Its Inspirations for Evolution of the Paleo-Pacific Ocean

by Qing Liu, Cui Liu, Jixu Liu, Jinfu Deng and Shipan Tian

Appl. Sci. 2025, 15(15), 8139; https://doi.org/10.3390/app15158139 (registering DOI) - 22 Jul 2025

Abstract

The Raohe subduction–accretion complex (RSAC) in the Wandashan Block, NE China, comprises ultramafic rocks, gabbro, mafic volcanic rocks, deep-sea and hemipelagic sediments, and trench–slope turbidites. We investigate the basalts within the RSAC to resolve debates on its origin. Zircon U-Pb dating of pillow [...] Read more.

The Raohe subduction–accretion complex (RSAC) in the Wandashan Block, NE China, comprises ultramafic rocks, gabbro, mafic volcanic rocks, deep-sea and hemipelagic sediments, and trench–slope turbidites. We investigate the basalts within the RSAC to resolve debates on its origin. Zircon U-Pb dating of pillow basalt from Dadingzi Mountain yields a concordant age of 117.5 ± 2.1 Ma (MSWD = 3.6). Integrating previous studies, we identify three distinct basalt phases. The Late Triassic basalt (210 Ma–230 Ma) is characterized as komatites–melilitite, exhibiting features of island arc basalt, as well as some characteristics of E-MORB. It also contains high-magnesium lava, suggesting that it may be a product of a juvenile arc. The Middle Jurassic basalt (around 159 Ma–172 Ma) consists of a combination of basalt and magnesium andesite, displaying features of oceanic island basalt and mid-ocean ridge basalt. Considering the contemporaneous sedimentary rocks as hemipelagic continental slope deposits, it is inferred that these basalts were formed in an arc environment associated with oceanic subduction, likely as a result of subduction of the young oceanic crust. The Early Cretaceous basalt (around 117 Ma) occurs in pillow structures, exhibiting some characteristics of oceanic island basalt but also showing transitional features towards a continental arc. Considering the regional distribution of the rocks, it is inferred that this basalt likely formed in a back-arc basin. Integrating the formation ages, nature, and tectonic attributes of the various structural units within the RSAC, as well as previous research, it is inferred that subduction of the Paleo-Pacific Ocean had already begun during the Late Triassic and continued into the Early Cretaceous without cessation. Full article

► Show Figures

Figure 1

13 pages, 4863 KiB

Open AccessArticle

p53 Protein Stability Plays a Crucial Role in NaB-Mediated Apoptosis in Colorectal Cancer Cells

by Jeong Yeon Lee and Hyunju Kim

Curr. Issues Mol. Biol. 2025, 47(8), 579; https://doi.org/10.3390/cimb47080579 (registering DOI) - 22 Jul 2025

Abstract

Colorectal cancer (CRC) is associated with factors such as an unhealthy diet, physical inactivity, obesity, diabetes, and chronic inflammatory conditions like inflammatory bowel disease (IBD), as well as TP53 mutations, which are observed in a broad spectrum of CRC. Additionally, alteration in the [...] Read more.

Colorectal cancer (CRC) is associated with factors such as an unhealthy diet, physical inactivity, obesity, diabetes, and chronic inflammatory conditions like inflammatory bowel disease (IBD), as well as TP53 mutations, which are observed in a broad spectrum of CRC. Additionally, alteration in the composition of the gut microbiome community and metabolism plays a significant role in the development of colorectal cancer and its therapeutic effects. It is well known that treatment with sodium butyrate (NaB), an intestinal microbial metabolite, can induce apoptosis by activating histone deacetylase (HDAC) in cancer cells. Therefore, this study examined the relationship between NaB-induced apoptosis and p53 protein level in colorectal cancer cells. Treatment with NaB triggered cell death in the HCT116 cell line. Furthermore, a notable elevation in p53 protein level was detected following treatment with a high concentration of NaB, compared to both the control group and the low concentration NaB. Furthermore, apoptotic cell death was diminished in a p53-deficient cell line (HCT 116 p53^−/−) and p53 protein expression was more stabilized. Although p53 mRNA expression was not affected, acetylation of p53 protein was clearly observed by high concentration NaB treatment. To demonstrate the relationship between p53 acetylation and cell death, HT29 cells were treated with a high concentration of NaB. In HT29 cells with a mutation in the p53 gene, increased cell viability, overproduction p53 protein, and hyperacetylation of p53 were observed compared to the control. The results of this study suggest that p53 protein expression plays an important role in the effectiveness of therapy utilizing gut microbiota metabolites. Full article

(This article belongs to the Section Molecular Medicine)

► Show Figures

Figure 1

18 pages, 2094 KiB

Open AccessSystematic Review

Scrotal Migration of the Ventriculoperitoneal Shunt in a 1-Year-Old Pediatric Patient: A Case Report and Systematic Literature Review

by Zenon Pogorelić, Stipe Ninčević, Vlade Babić, Miro Jukić and Stipe Vidović

J. Clin. Med. 2025, 14(15), 5183; https://doi.org/10.3390/jcm14155183 (registering DOI) - 22 Jul 2025

Abstract

Background: Migration of the peritoneal end of the ventriculoperitoneal shunt (VPS) into the scrotum is a rare but recognized complication. Inguinoscrotal migration typically occurs as a result of increased intra-abdominal pressure combined with a patent processus vaginalis. A 14-month-old pediatric patient presented to [...] Read more.

Background: Migration of the peritoneal end of the ventriculoperitoneal shunt (VPS) into the scrotum is a rare but recognized complication. Inguinoscrotal migration typically occurs as a result of increased intra-abdominal pressure combined with a patent processus vaginalis. A 14-month-old pediatric patient presented to the emergency department with abdominal pain, vomiting, and swelling of the right scrotum that had persisted for two days. The patient had a history of a head injury that had resulted in a large secondary arachnoid cyst for which a VPS had been placed at eight months of age. Examination of the inguinoscrotal region revealed a swollen and painful right side of the scrotum with a hydrocele and a palpable distal portion of the ventriculoperitoneal catheter in the right groin extending to the scrotum. After a brief preoperative preparation, the patient underwent laparoscopic abdominal emergency exploration, during which shunt repositioning and laparoscopic closure of the patent processus vaginalis were performed. Methods: A systematic review was conducted in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. Results: A total of 30 case reports and six case series were included, analyzing 52 pediatric patients with scrotal migration of the VPS. The median age at presentation was 24 months (range: 1–169 months). The indication for VPS placement was hydrocephalus. Migration of the VPS catheter occurred on the right side in 34 cases. The median interval from VPS placement to the onset of symptoms was 9.0 months (range: 1 day–72 months). The most frequently reported symptoms were scrotal/inguinoscrotal swelling (n = 50), vomiting (n = 7), and fever (n = 3). Diagnostic methods included abdominal X-ray (n = 43), ultrasound (n = 5), scrotal transillumination test (n = 5), and computed tomography (n = 1). Regarding treatment, surgical repositioning of the VPS catheter into the peritoneal cavity was performed in 47 patients (90.4%), with no intraoperative or postoperative complications reported. Conclusions: Laparoscopic repositioning of the VPS into the peritoneal cavity, combined with closure of the processus vaginalis, appears to be a safe and effective treatment option for scrotal migration of the VPS. However, further well-designed studies are warranted to provide more comprehensive, generalizable, and unbiased evidence regarding this complication in the pediatric population. Full article

(This article belongs to the Special Issue Latest Advances in Pediatric Surgery)

► Show Figures

Figure 1

15 pages, 3017 KiB

Open AccessArticle

Strategies for the Recovery of Tungsten from Wolframite, Scheelite, or Wolframite–Scheelite Mixed Concentrates of Spanish Origin

by Francisco Jose Alguacil, Manuel Alonso, Luis Javier Lozano and Jose Ignacio Robla

Metals 2025, 15(8), 819; https://doi.org/10.3390/met15080819 (registering DOI) - 22 Jul 2025

Abstract

Among the strategic materials considered by the EU, tungsten is included; thus, investigations about the recovery of this metal both from natural and recyclable sources are of interest. In this work, we presented an investigation about the recovery of tungsten based on the [...] Read more.

Among the strategic materials considered by the EU, tungsten is included; thus, investigations about the recovery of this metal both from natural and recyclable sources are of interest. In this work, we presented an investigation about the recovery of tungsten based on the treatment of three tungsten-bearing concentrates: scheelite (29% W), wolframite (50% W), and mixed scheelite–wolframite (29% W). All of these come from a cassiterite ore of Spanish origin. The characteristics of each concentrate pave the procedure to be followed in each case. In the case of the wolframite concentrate, the best results were derived from the leaching of the ore with NaOH solutions, whereas the treatment of the scheelite concentrate benefits from an acidic (HCl) leaching. The attack of the mixed concentrate is only possible by a previous roasting step (sodium carbonate and 700–800 °C) followed by a leaching step with water. In the acidic leaching, tungstic acid (H₂WO₄) was obtained, and the alkaline–water leaching produces Na₂WO₄ solutions from which pure synthesized scheelite is precipitated. Full article

► Show Figures

Figure 1

10 pages, 2670 KiB

Open AccessArticle

High-Temperature-Resistant High-Entropy Oxide Protective Coatings for Piezoelectric Thin Films

by Huayong Hu, Jie Liu, Liqing Chao, Xiangdong Ma, Jun Zhang, Yanbing Zhang and Bing Yang

Coatings 2025, 15(8), 861; https://doi.org/10.3390/coatings15080861 (registering DOI) - 22 Jul 2025

Abstract

By introducing oxygen doping, the structure of an AlCrNbSiTiN coating was optimized, and its high-temperature oxidation resistance was improved. As the oxygen content incorporated increases, the coating changes from an FCC structure to an amorphous or spinel structure. Meanwhile, stress relaxation occurred, and [...] Read more.

By introducing oxygen doping, the structure of an AlCrNbSiTiN coating was optimized, and its high-temperature oxidation resistance was improved. As the oxygen content incorporated increases, the coating changes from an FCC structure to an amorphous or spinel structure. Meanwhile, stress relaxation occurred, and the hardness of the coating dropped to 12 gpa. Oxygen-doped coatings exhibit excellent oxidation resistance; this is especially the case for oxidized coatings, whose structure remains stable up to 900 °C in an oxidizing environment. Full article

(This article belongs to the Special Issue Advanced Thin Films of High-Entropy Alloys)

► Show Figures

Figure 1

19 pages, 6555 KiB

Open AccessArticle

Exploiting Structured Global and Neighbor Orders for Enhanced Ordinal Regression

by Imam Mustafa Kamal, Solichin Mochammad, Latifah Nurahmi, Azis Natawijaya and Muhammad Kalili

Information 2025, 16(8), 624; https://doi.org/10.3390/info16080624 (registering DOI) - 22 Jul 2025

Abstract

Ordinal regression combines classification and regression techniques, constrained by the intrinsic order among categories. It has wide-ranging applications in real-world scenarios, such as product quality grading, medical diagnoses, and facial age recognition, where understanding ranked relationships is crucial. Existing models, which often employ [...] Read more.

Ordinal regression combines classification and regression techniques, constrained by the intrinsic order among categories. It has wide-ranging applications in real-world scenarios, such as product quality grading, medical diagnoses, and facial age recognition, where understanding ranked relationships is crucial. Existing models, which often employ a series of binary classifiers with ordinal consistency loss, effectively enforce global order consistency but frequently encounter misclassification errors between adjacent categories. Achieving both global and local (neighbor-level) ordinal consistency, however, remains a significant challenge. In this study, we propose a hybrid ordinal regression model that addresses global ordinal structure while enhancing local consistency between neighboring categories. Our approach leverages ordinal metric learning to generate embeddings that capture global ordinal relationships and extends consistent rank logits with a neighbor order penalty in the loss function to reduce adjacent category misclassifications. Experimental results on multiple benchmark ordinal datasets demonstrate that our model significantly minimizes neighboring misclassification errors and global order inconsistencies, outperforming existing ordinal regression models. Full article

(This article belongs to the Special Issue Application of Machine Learning in Data Science and Computational Intelligence)

► Show Figures

Figure 1

24 pages, 5200 KiB

Open AccessArticle

DRFAN: A Lightweight Hybrid Attention Network for High-Fidelity Image Super-Resolution in Visual Inspection Applications

by Ze-Long Li, Bai Jiang, Liang Xu, Zhe Lu, Zi-Teng Wang, Bin Liu, Si-Ye Jia, Hong-Dan Liu and Bing Li

Algorithms 2025, 18(8), 454; https://doi.org/10.3390/a18080454 (registering DOI) - 22 Jul 2025

Abstract

Single-image super-resolution (SISR) plays a critical role in enhancing visual quality for real-world applications, including industrial inspection and embedded vision systems. While deep learning-based approaches have made significant progress in SR, existing lightweight SR models often fail to accurately reconstruct high-frequency textures, especially [...] Read more.

Single-image super-resolution (SISR) plays a critical role in enhancing visual quality for real-world applications, including industrial inspection and embedded vision systems. While deep learning-based approaches have made significant progress in SR, existing lightweight SR models often fail to accurately reconstruct high-frequency textures, especially under complex degradation scenarios, resulting in blurry edges and structural artifacts. To address this challenge, we propose a Dense Residual Fused Attention Network (DRFAN), a novel lightweight hybrid architecture designed to enhance high-frequency texture recovery in challenging degradation conditions. Moreover, by coupling convolutional layers and attention mechanisms through gated interaction modules, the DRFAN enhances local details and global dependencies with linear computational complexity, enabling the efficient utilization of multi-level spatial information while effectively alleviating the loss of high-frequency texture details. To evaluate its effectiveness, we conducted ×4 super-resolution experiments on five public benchmarks. The DRFAN achieves the best performance among all compared lightweight models. Visual comparisons show that the DRFAN restores more accurate geometric structures, with up to +1.2 dB/+0.0281 SSIM gain over SwinIR-S on Urban100 samples. Additionally, on a domain-specific rice grain dataset, the DRFAN outperforms SwinIR-S by +0.19 dB in PSNR and +0.0015 in SSIM, restoring clearer textures and grain boundaries essential for industrial quality inspection. The proposed method provides a compelling balance between model complexity and image reconstruction fidelity, making it well-suited for deployment in resource-constrained visual systems and industrial applications. Full article

► Show Figures

Figure 1

26 pages, 2029 KiB

Open AccessArticle

Illegal Waste Dumps and Water Quality: Environmental and Logistical Challenges for Sustainable Development—A Case Study of the Ružín Reservoir (Slovakia)

by Oľga Glova Végsöová and Martin Straka

Environments 2025, 12(8), 251; https://doi.org/10.3390/environments12080251 (registering DOI) - 22 Jul 2025

Abstract

The aim of the article is to highlight the increasing environmental burden on aquatic ecosystems in Slovakia due to continuous pollution from municipal, industrial and agricultural sources. Laboratory analyses have shown alarming exceedance of the limit values of contaminants, with nitrate nitrogen (NO [...] Read more.

The aim of the article is to highlight the increasing environmental burden on aquatic ecosystems in Slovakia due to continuous pollution from municipal, industrial and agricultural sources. Laboratory analyses have shown alarming exceedance of the limit values of contaminants, with nitrate nitrogen (NO₃⁻) reaching 5.8 mg/L compared to the set limit of 2.5 mg/L and phosphorus concentrations exceeding the permissible values by a factor of five, thereby escalating the risk of eutrophication and loss of ecological stability of the aquatic ecosystem. The accumulation of heavy metals is also a problem—lead (Pb) concentrations reach up to 9.7 μg/L, which exceeds the safe limit by a factor of ten. Despite the measures implemented, such as scum barriers, there is continuous contamination of the aquatic environment, with illegal waste dumps and uncontrolled runoff of agrochemicals playing a significant role. The research results underline the critical need for a more effective environmental policy and more rigorous monitoring of toxic substances in real time. These findings highlight not only the urgency of more effective environmental policy and stricter real-time monitoring of toxic substances, but also the necessity of integrating environmental logistics into the design of sustainable solutions. Logistical approaches including the optimization of waste collection, coordination of stakeholders and creation of infrastructural conditions can significantly contribute to reducing environmental burdens and ensure the continuity of environmental management in ecologically sensitive areas. Full article

► Show Figures

Figure 1

24 pages, 2758 KiB

Open AccessArticle

A Techno-Economic Analysis of Integrating an Urban Biorefinery Process Within a Wastewater Treatment Plant to Produce Sustainable Wood Adhesives

by Blake Foret, William M. Chirdon, Rafael Hernandez, Dhan Lord B. Fortela, Emmanuel Revellame, Daniel Gang, Jalel Ben Hmida, William E. Holmes and Mark E. Zappi

Sustainability 2025, 17(15), 6679; https://doi.org/10.3390/su17156679 (registering DOI) - 22 Jul 2025

Abstract

Societies are aiming to have a higher ecological consciousness in wastewater treatment operations and achieve a more sustainable future. With this said, global demands for larger quantities of resources and the consequent waste generated will inevitably lead to the exhaustion of current municipal [...] Read more.

Societies are aiming to have a higher ecological consciousness in wastewater treatment operations and achieve a more sustainable future. With this said, global demands for larger quantities of resources and the consequent waste generated will inevitably lead to the exhaustion of current municipal wastewater treatment works. The utilization of biosolids (particularly microbial proteins) from wastewater treatment operations could generate a sustainable bio-adhesive for the wood industry, reduce carbon footprint, mitigate health concerns related to the use of carcinogenic components, and support a more circular economic option for wastewater treatment. A techno-economic analysis for three 10 MGD wastewater treatment operations producing roughly 11,300 dry pounds of biosolids per day, in conjunction with co-feedstock defatted soy flour protein at varying ratios (i.e., 0%, 15%, and 50% wet weight), was conducted. Aspen Capital Cost Estimator V12 was used to design and estimate installed equipment additions for wastewater treatment plant integration into an urban biorefinery process. Due to the mechanical attributes and market competition, the chosen selling prices of each adhesive per pound were set for analysis as USD 0.75 for Plant Option P1, USD 0.85 for Plant Option P2, and USD 1.00 for Plant Option P3. Over a 20-year life, each plant option demonstrated economic viability with high NPVs of USD 107.9M, USD 178.7M, and USD 502.2M and internal rates of return (IRRs) of 24.0%, 29.0%, and 44.2% respectively. The options examined have low production costs of USD 0.14 and USD 0.19 per pound, minimum selling prices of USD 0.42–USD 0.51 per pound, resulting in between 2- and 4-year payback periods. Sensitivity analysis shows the effects biosolid production fluctuations, raw material market price, and adhesive selling price have on economics. The results proved profitable even with large variations in the feedstock and raw material prices, requiring low market selling prices to reach the hurdle rate of examination. This technology is economically enticing, and the positive environmental impact of waste utilization encourages further development and analysis of the bio-adhesive process. Full article

(This article belongs to the Special Issue Development in Sustainable Water Management: Processes, Barriers, Protection and Future Perspectives)

► Show Figures

Figure 1

29 pages, 4032 KiB

Open AccessArticle

Advancing Sustainability in Aerospace: Evaluating the Performance of Recycled Carbon Fibre Composites in Aircraft Wing Spar Design

by Naomi Bouman, Sofia Salles Lantyer Marques, Naiara Poli Veneziani Sebbe, Antoine Gerritse, Heide Heloise Bernardi, William Marcos Muniz Menezes, Francisco José Gomes da Silva, Jorge Tadao Matsushima, Lucas Giovanetti and Rita de Cássia Mendonça Sales-Contini

J. Compos. Sci. 2025, 9(8), 384; https://doi.org/10.3390/jcs9080384 (registering DOI) - 22 Jul 2025

Abstract

The aerospace industry is increasingly turning to composite materials due to their exceptional strength, stiffness, and beneficial physical properties. However, increased reliance on carbon fibre composites has substantial environmental implications, particularly concerning waste management. Recycling these materials is a potential solution to these [...] Read more.

The aerospace industry is increasingly turning to composite materials due to their exceptional strength, stiffness, and beneficial physical properties. However, increased reliance on carbon fibre composites has substantial environmental implications, particularly concerning waste management. Recycling these materials is a potential solution to these sustainability issues, provided the recycled fibres retain adequate mechanical strength and durability. This study evaluates the mechanical capabilities of recycled carbon fibres in a scaled-down aircraft spar model (AMT-600 GURI), contrasting them with the capabilities of conventional spars. The primary objective is to ascertain whether recycled composites can fulfil the stringent structural requirements of aerospace applications, employing both simulation and experimental validation methods. The recycled carbon fibre composites were manufactured using hand lay-up and vacuum bagging techniques, and their properties were validated through rigorous tensile and compressive strength testing. These validated results were then used to inform a finite element model developed in HyperWorks software. Simulations revealed that the recycled spar achieved maximum stress values of 3.87 MPa under lift forces, a slight increase of +8.95% compared to the original spar, and 55.05 MPa under drag forces, a significant improvement of +36%. Aerodynamic evaluations further confirmed the structural resilience of the recycled spar, with displacement measurements of 141.4 mm for lift and 504.8 mm for drag, closely aligning with the original spar’s performance. In summary, this study demonstrates that recycled carbon fibre composites can serve as effective substitutes for traditional aerospace materials, thereby supporting sustainability initiatives without compromising performance. The outlined approach provides a reliable framework for incorporating recycled materials. Full article

(This article belongs to the Collection Editorial Board Members’ Collection Series: Fibre Composite Materials)

► Show Figures

Figure 1

Recent Articles

Open Access Journals

Highly Accessed Articles

Latest Books

News

Blog Posts

Topics

Selected Special Issues

Selected Collections

Journals by Subject

Further Information

Guidelines

MDPI Initiatives

Follow MDPI