Journal Description
Horticulturae
Horticulturae
is an international, peer-reviewed, open access journal published monthly online by MDPI. The Spanish Society of Horticultural Sciences (SECH) is affiliated with Horticulturae and its members receive a discount on the article processing charges.
- Open Access— free for readers, with article processing charges (APC) paid by authors or their institutions.
- High Visibility: indexed within Scopus, SCIE (Web of Science), PubAg, AGRIS, FSTA, and other databases.
- Journal Rank: JCR - Q1 (Horticulture) / CiteScore - Q2 (Horticulture)
- Rapid Publication: manuscripts are peer-reviewed and a first decision is provided to authors approximately 14.7 days after submission; acceptance to publication is undertaken in 2.6 days (median values for papers published in this journal in the second half of 2023).
- Recognition of Reviewers: reviewers who provide timely, thorough peer-review reports receive vouchers entitling them to a discount on the APC of their next publication in any MDPI journal, in appreciation of the work done.
Impact Factor:
3.1 (2022);
5-Year Impact Factor:
3.4 (2022)
Latest Articles
Phytoene Desaturase (PDS) Gene-Derived Markers Identify “A” and “B” Genomes in Banana (Musa spp.)
Horticulturae 2024, 10(3), 294; https://doi.org/10.3390/horticulturae10030294 - 19 Mar 2024
Abstract
Phytoene desaturase (PDS) is a plant enzyme involved in carotenoid biosynthesis. The PDS gene has been used as a selective marker for genome editing in several plant species, including banana (Musa spp.). Its knockout promotes dwarfism and albinism, characteristics that are easily
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Phytoene desaturase (PDS) is a plant enzyme involved in carotenoid biosynthesis. The PDS gene has been used as a selective marker for genome editing in several plant species, including banana (Musa spp.). Its knockout promotes dwarfism and albinism, characteristics that are easily recognizable and highly favorable. In Musa spp., the A genome increases fruit production and quality, whereas the B genome is associated with tolerance to biotic and abiotic stresses. The objective of this study was to identify a molecular marker in the PDS gene to easily discriminate the A and B genomes of banana. A 2166 bp fragment for the “PDSMa” marker was identified as polymorphic for the A genome (identification accuracy of 99.33%), whereas ~332 and ~225 bp fragments were detected for the “PDSMb” marker with 100% accuracy using MedCalc software. In this study, we used genotypes with A and B genomes that are used in the genetic improvement of bananas and an accession with the BT genome. It was not possible to differentiate the accession with the BT genome from the others, suggesting that the markers do not have the capacity to separate the T genome from the A and B genomes. To the best of our knowledge, this is the first study to use the PDS gene to determine doses of the A genome and identify the B genome in Musa spp., which will aid in evaluating the genomic constitution of banana hybrids and accessions at the seedling stage and accelerating their classification in crop genetic improvement programs.
Full article
(This article belongs to the Special Issue Developments in the Genetics and Breeding of Banana Species)
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Open AccessArticle
De Novo Transcriptome Analysis by PacBio SMRT-Seq and Illumina RNA-Seq Provides New Insights into Polyphenol Biosynthesis in Chinese Olive Fruit
by
Qinghua Ye, Shiyan Zhang, Qian Xie, Wei Wang, Zhehui Lin, Huiquan Wang, Yafang Yuan and Qingxi Chen
Horticulturae 2024, 10(3), 293; https://doi.org/10.3390/horticulturae10030293 - 19 Mar 2024
Abstract
Polyphenols play a crucial role in fruit flavor. To elucidate the mechanism of fruit polyphenol metabolism, we constructed a transcriptome atlas through PacBio single-molecule real-time (SMRT) sequencing and Illumina next-generation sequencing (NGS) using Canarium album (Lour.) Raeusch., which is a fantastic fruit rich
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Polyphenols play a crucial role in fruit flavor. To elucidate the mechanism of fruit polyphenol metabolism, we constructed a transcriptome atlas through PacBio single-molecule real-time (SMRT) sequencing and Illumina next-generation sequencing (NGS) using Canarium album (Lour.) Raeusch., which is a fantastic fruit rich in polyphenolic compounds. In this work, PacBio full-length transcriptome assembly generated 135,439 isoforms with an average length of all isoforms of 2687.94 bp and an N50 length of 3224 bp. To gain deeper insights into the molecular mechanisms of polyphenol biosynthesis in C. album, we constructed twelve RNA-Seq libraries from four developmental stages of the fruits. We identified a total of 28,658 differentially expressed genes (DEGs). We found that many DEGs were involved in metabolic pathways, biosynthesis of secondary metabolites, biosynthesis of antibiotics, starch and sucrose metabolism, and plant hormone signal transduction. Here, we report the expression profiles of 215 DEGs encoding 27 enzymes involved in the polyphenol biosynthesis pathway in C. album. In addition, 285 differentially expressed transcription factors (TFs) continuously down-regulated in four developmental periods of C. album fruit, which may indicate their potential role in the response to polyphenol metabolism and phenylpropanoid biosynthesis pathways. This report will help us understand polyphenol biosynthesis’s functions and metabolic mechanism in C. album. The transcriptome data provide a valuable resource for genetic and genomics research. They will facilitate future work exploiting C. album and other fruits used as medicine and food.
Full article
(This article belongs to the Section Genetics, Genomics, Breeding, and Biotechnology (G2B2))
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Open AccessReview
Potato Biofortification: A Systematic Literature Review on Biotechnological Innovations of Potato for Enhanced Nutrition
by
Smita Agrawal, Amit Kumar, Yash Gupta and Ayushi Trivedi
Horticulturae 2024, 10(3), 292; https://doi.org/10.3390/horticulturae10030292 - 19 Mar 2024
Abstract
Potato biofortification is a comprehensive approach aimed at enhancing the nutritional content of potatoes, addressing widespread nutrient deficiencies and contributing to global food security. This systematic review examines the existing literature on various aspects of potato biofortification, encompassing genetic, agronomic, and biotechnological strategies.
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Potato biofortification is a comprehensive approach aimed at enhancing the nutritional content of potatoes, addressing widespread nutrient deficiencies and contributing to global food security. This systematic review examines the existing literature on various aspects of potato biofortification, encompassing genetic, agronomic, and biotechnological strategies. The review highlights the nutritional significance of potatoes, emphasizing their role as a staple food in many regions. Genetic approaches to biofortification involve the identification and use of natural variations in potato germplasm to develop varieties with elevated levels of essential nutrients. This includes targeting key micronutrients, such as iron, zinc, and vitamins, through traditional breeding methods. The review explores the genetic diversity within potato germplasm and the potential for breeding programs to develop nutrient-rich varieties. Agronomic practices play a crucial role in potato biofortification, with studies demonstrating the impact of tuber priming and the application of mineral fertilizers on nutrient concentrations in potatoes. The review delves into the intricacies of agronomic biofortification, emphasizing the importance of precise dosages and timing for optimal results. Biotechnological tools, including transgenic and non-transgenic approaches, are discussed in the context of potato biofortification. The review evaluates the efficiency and ethical considerations associated with the development of biofortified transgenic potatoes and emphasizes the significance of non-transgenic approaches in addressing consumer concerns and regulatory barriers. Overall, this systematic review provides a comprehensive overview of the current state of potato biofortification research. It synthesizes findings from diverse studies, offering insights into the potential of biofortified potatoes to address hidden hunger and contribute to improved nutritional outcomes. This review also identifies knowledge gaps and areas for future research, guiding the direction of efforts to harness the full potential of potato biofortification for global food and nutrition security.
Full article
(This article belongs to the Section Plant Nutrition)
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Open AccessArticle
Integrative Metabolome and Transcriptome Analyses Reveal the Pericarp Coloration Mechanisms in Bitter Melon (Momordica charantia L.)
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Liang Yang, Zhi Li, Ju Li, Yanqin Ma, Mingjun Miao, Haicheng Long, Yujie Zhou and Wei Chang
Horticulturae 2024, 10(3), 291; https://doi.org/10.3390/horticulturae10030291 - 18 Mar 2024
Abstract
Pericarp colors are critical agronomic traits that affect the quality and economic values of fruits. Although a diversity of bitter melon pericarp (BMP) colors is available, the fruit pigmentation mechanisms remain elusive. Hence, this study aimed to unveil the key metabolites and molecular
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Pericarp colors are critical agronomic traits that affect the quality and economic values of fruits. Although a diversity of bitter melon pericarp (BMP) colors is available, the fruit pigmentation mechanisms remain elusive. Hence, this study aimed to unveil the key metabolites and molecular mechanisms underlying variation in BMP coloration through integrative metabolomics and transcriptomics analyses of four differently colored genotypes, including K1102 (grayish orange), 262 (grayish yellow), 1392 (very soft green), and K115 (dark grayish cyan). The four BMPs exhibited significant metabolite profile and transcriptional differences, as over 112 and 1865 DAMs (differentially accumulated metabolites) and DEGs (differentially expressed genes), respectively, were identified. The variation in the content of six anthocyanins, including malvidin 3-O-glucoside, petunidin 3-O-glucoside, rosinidin O-hexoside, cyanidin, cyanidin 3-p-hydroxybenzoylsophoroside-5-glucoside, and pelargonidin 3-O-beta-D-glucoside, might be the major driving factor of BMP color changes. Notably, malvidin 3-O-glucoside, rosinidin O-hexoside, and petunidin 3-O-glucoside are the dominant pigments in K115, while carotenoids and other flavonoids may contribute to other colors. Candidate flavonoid structural and regulatory (MYBs, NACs, MADSs, bHLHs, and bZIPs) genes were identified. Of them, gene13201 (anthocyanin reductase), gene8173 (polyphenol oxidase), gene2136 (NAC43), gene19593 (NAC104), and gene15171 (tetrapyrrole-binding protein) might play essential roles in K115 pericarp color development. Our findings deepen our understanding of BMP pigmentation and provide fundamental resources for higher-valued bitter melon breeding perspectives.
Full article
(This article belongs to the Special Issue The Physiology, Molecular Biology, Biochemistry and Photoсhemistry in Horticultural Plant)
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Open AccessArticle
Physiological and Transcriptional Characteristics of Banana Seedlings in Response to Nitrogen Deficiency Stress
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Lei Zhao, Bingyu Cai, Xiaohan Zhang, Bencheng Zhang, Junting Feng, Dengbo Zhou, Yufeng Chen, Miaoyi Zhang, Dengfeng Qi, Wei Wang, Jianghui Xie and Yongzan Wei
Horticulturae 2024, 10(3), 290; https://doi.org/10.3390/horticulturae10030290 - 18 Mar 2024
Abstract
Nitrogen is a crucial element for the growth and development of plants, directly affecting crop growth and yield. To investigate the physiological and molecular mechanism of nitrogen-deficiency stress, we conducted an investigation into the effects of different nitrogen levels on the growth, photosynthetic
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Nitrogen is a crucial element for the growth and development of plants, directly affecting crop growth and yield. To investigate the physiological and molecular mechanism of nitrogen-deficiency stress, we conducted an investigation into the effects of different nitrogen levels on the growth, photosynthetic characteristics, and gene transcription levels of banana seedlings. Compared with the control group with normal nitrogen levels (NN), the height of plants receiving Reduced-N (NR), Low-N (LN), and N-Free (NF) treatments was decreased by 0.45 cm, 2.5 cm, and 3.25 cm, respectively. Their dry weight was reduced by 1.63 g, 2.99 g, and 2.88 g, respectively. Conversely, the dry weight of the underground plant part in the LN and NF treatment groups exhibited an increase of 0.13 g and 0.16 g, respectively. Regarding photosynthetic characteristics, the Specialty Products Agricultural Division (SPAD) values of the NR, LN, and NF treatments showed reductions of 15.5%, 30.4%, and 35.9%, respectively, compared with those of the control treatments. The values of maximum photosynthetic efficiency (Fv/Fm), actual photosynthetic efficiency (Y(Ⅱ)), and relative electron transfer (ETR) of the banana seedlings decreased to different degrees after NR, LN, and NF treatment, and their values were positively correlated with N levels. Gene transcription analysis showed that N transport-related proteins, including NRT1.7, NRT2.3a, NRT2.3b, and NRT2.5, were significantly up-regulated to increase the nitrogen absorption capacity of plant roots. On the other hand, various transcription factors including GRAS, MYB, and WRKY were notably up-regulated, facilitating root growth and the expanding root absorption area, thereby enhancing nitrogen uptake. Furthermore, genes associated with endogenous hormone metabolic pathways such as gibberellin (GA), strigolactone (SL), and brassinosteroids (BR) were activated in banana plants subjected to low nitrogen stress, enhancing the plant’s ability to adapt to nitrogen-deficient conditions. These findings offer valuable insights into understanding the transcriptional regulatory mechanisms governing banana responses to low nitrogen stress and breeding new varieties with improved nutrient utilization.
Full article
(This article belongs to the Special Issue Horticultural Plant Nutrition, Fertilization, Soil Management)
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Open AccessArticle
Effect of Seed Spaceflight Storage on Tomato Fruit Quality and Peel/Pulp Mineral and Antioxidant Distribution
by
Nadezhda Golubkina, Elena Dzhos, Maria Bogachuk, Marina Antoshkina, Olga Verba, Tatiana Zavarykina, Galina Nechitailo, Otilia Cristina Murariu, Alessio Vincenzo Tallarita and Gianluca Caruso
Horticulturae 2024, 10(3), 289; https://doi.org/10.3390/horticulturae10030289 - 18 Mar 2024
Abstract
The spaceflight storage of seeds is known to cause mutations affecting both their quality and the mature plants originating from them. To study the effects of space stress, tomato seeds of two cultivars (Lotus and Autumn rhapsody) were subjected to half a year
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The spaceflight storage of seeds is known to cause mutations affecting both their quality and the mature plants originating from them. To study the effects of space stress, tomato seeds of two cultivars (Lotus and Autumn rhapsody) were subjected to half a year of storage at the International Space Station (ISS), and then, sown in a greenhouse to produce tomato fruits. The space-treated plants gave smaller fruits with a stable total yield not significantly different from that of the control plants. Space-treated tomatoes showed significantly higher levels of dry matter, dietary fiber, monosaccharides and citric and malic acids and lower values of oxalic acid compared to the control plants. The pulp of space-treated fruits had 1.44–1.70 times lower levels of carotenoids, while their peel contained a 1.27–1.90 times higher pigment amount compared to the control plants. No significant changes in the total antioxidant activity (AOA), photosynthetic pigments and phenolic (TP) and proline content were recorded in the fruits due to seed spaceflight storage. Contrarily, space-treated tomatoes showed decreased levels of Ca, Sr and Mo and increased Se both in the fruit pulp and peel. The concentration of Fe and especially Pb was lower in space-treated fruit pulp. Positive correlations between Se and dry matter, Ca and Sr, Ca and Co, Ca and Fe, and Cr and carotenoids, and negative correlations between Se and Mo, Se and K, and Mo and dry matter were recorded. The results indicate that seed stress caused by long-term spaceflight affects both the biochemical characteristics and mineral composition of tomato fruits and causes the peel/pulp redistribution of carotenoids as well as macro- and micro-elements, improving Se accumulation levels in the fruit peel.
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(This article belongs to the Special Issue Indoor Farming and Artificial Cultivation)
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Open AccessArticle
Comparison of the Differences in Tolerance to Drought Stress across Five Clematis Species Based on Seed Germination and Seedling Growth
by
Zhu Zhu, Yongmei Li, Ting Liu, Rongrong Shi, Xiangyun Xu, Zhanbang Song and Yanli Wang
Horticulturae 2024, 10(3), 288; https://doi.org/10.3390/horticulturae10030288 - 17 Mar 2024
Abstract
Plants of the Clematis genus are attractive ornamental plants due to their various flower colors and patterns, and they play an important role as ground cover plants in landscaping. However, the application of Clematis plants in landscaping in arid and semi-arid areas is
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Plants of the Clematis genus are attractive ornamental plants due to their various flower colors and patterns, and they play an important role as ground cover plants in landscaping. However, the application of Clematis plants in landscaping in arid and semi-arid areas is limited. This study used five common wild Clematis species in Gansu Province as experimental materials, including Clematistangutica, Clematis glauca, Clematis intricata, Clematis nannophylla, and Clematis fruticosa. By simulating different intensities of drought stress with polyethylene glycol (PEG), the germination behavior of seeds and the physiological and biochemical responses of seedlings of these five species to drought stress were observed. The results showed that 15% PEG stress significantly inhibited the seed germination of the five species, which was also the drought threshold for seed germination of C. fruticosa. C. tangutica exhibited the strongest tolerance to drought stress in seed germination. Seedlings of the five Clematis plants showed different levels of tolerance to drought stress, mainly demonstrating higher tolerance to 10% and 20% concentrations of PEG stress, while a 30% concentration of PEG stress caused varying degrees of damage to the seedlings of the five Clematis species. PCA analysis indicated that seedlings of C. intricata and C. glauca had higher scores under drought stress. These findings can provide a theoretical basis for the selection of urban landscaping plant species in arid and semi-arid regions of northwest China.
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(This article belongs to the Section Biotic and Abiotic Stress)
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Open AccessArticle
LbHSP17.9 Participated in the Regulation of Cold Stress in Cut Lily Flowers
by
Jiahui Zhao, Xinyu Yan, Wei Huang, Cheng Liu, Xuan Hao, Chengye Gao, Minghua Deng and Jinfen Wen
Horticulturae 2024, 10(3), 287; https://doi.org/10.3390/horticulturae10030287 - 17 Mar 2024
Abstract
Heat shock proteins (HSPs) play important roles in plant stress resistance, but it is not clear whether small molecular HSPs (sHSPs) are involved in the cold stress resistance of lily flowers. In this study, we cloned LbHSP17.9 and found that its expression was
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Heat shock proteins (HSPs) play important roles in plant stress resistance, but it is not clear whether small molecular HSPs (sHSPs) are involved in the cold stress resistance of lily flowers. In this study, we cloned LbHSP17.9 and found that its expression was up-regulated under cold stress. When LbHSP17.9 was silenced (TRV2::LbHSP17.9) using virus-induced gene silencing in cut lily flowers, the content of malondialdehyde was increased under 4 °C stress treatment. The catalase (CAT) activity in TRV2::LbHSP17.9 was significantly lower than in TRV2 in the first 7 days, and the peroxidase (POD) activity in TRV2::LbHSP17.9 was significantly lower than in TRV2 after 4 days of 4 °C stress. Further analysis showed that the transcription levels of LbCu/ZnSOD, LbMnSOD and LbCAT in TRV2::LbHSP17.9 were lower than those of TRV2 under 4 °C stress. When LbHSP17.9 was overexpressed in lily petal disks, the OE-LbHSP17.9 disks faded later than the controls at 4 °C and the relative conductivity decreased significantly. Overexpression of LbHSP17.9 in Arabidopsis thaliana resulted in fewer injury symptoms and lower MDA content than wild type under 4 °C stress. Therefore, we speculate that LbHSP17.9 can improve the resistance of lily flowers to cold stress.
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(This article belongs to the Section Biotic and Abiotic Stress)
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Open AccessArticle
Integrative Phytohormone and Transcriptome Analyses Reveal the Inhibitory Mechanism of Ethylene on Potato Tuber Sprouting at Room Temperature
by
Meixue Zhang, Wenxiao Jiao, Qingmin Chen, Maorun Fu and Cong Han
Horticulturae 2024, 10(3), 286; https://doi.org/10.3390/horticulturae10030286 - 17 Mar 2024
Abstract
As a commercial potato sprout suppressant, ethylene (Eth) is usually used under a low temperature for long-term storage of potato tubers. However, in many cases, potato tubers are usually transferred from a refrigeration house and sold at room temperature. In the present research,
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As a commercial potato sprout suppressant, ethylene (Eth) is usually used under a low temperature for long-term storage of potato tubers. However, in many cases, potato tubers are usually transferred from a refrigeration house and sold at room temperature. In the present research, Eth’s inhibitory effects on tuber sprouting at room temperature were investigated. The potential molecular mechanisms of Eth-induced sprout suppression were revealed by phytohormone and transcriptome analyses. Results showed that exogenous Eth significantly suppressed sprout growth in potato tubers during two weeks of storage. The endogenous plant hormone levels of abscisic acid (ABA) and auxin (Aux) were markedly reduced by Eth treatment. Transcriptomic analysis revealed that more transcriptional changes occurred in the early stage of sprouting. The differentially expressed genes (DEGs) assigned to the pathways of plant hormone signal transduction, photosynthesis, starch and sucrose metabolism and phenylpropanoid biosynthesis, which were closely associated with sprouting, were extensively down-regulated by Eth treatment. In addition, the heat map of six hormone signal transduction pathways showed that the expression of most DEGs annotated into the Aux signal transduction pathway was suppressed by Eth treatment, while the expression of many DEGs annotated as ETR (Eth receptor) and ERF1/2 (Eth-responsive transcription factor 1 and 2) in the Eth signal transduction pathway was enhanced by Eth treatment. Taken together, our results indicated that Eth-induced sprout inhibition might be closely related to the suppression of internal Aux production and signal transduction and the activation of Eth signal transduction.
Full article
(This article belongs to the Special Issue Postharvest Physiology and Quality Maintaining of Horticultural Plants)
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Open AccessFeature PaperArticle
Seed Morphological Analysis in Species of Vitis and Relatives
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José Javier Martín-Gómez, José Luis Rodríguez-Lorenzo, Diego Gutiérrez del Pozo, Félix Cabello Sáez de Santamaría, Gregorio Muñoz-Organero, Ángel Tocino and Emilio Cervantes
Horticulturae 2024, 10(3), 285; https://doi.org/10.3390/horticulturae10030285 - 16 Mar 2024
Abstract
Seed shape descriptions of species of Vitis have traditionally been based on adjectives comparing overall shape with geometric figures, such as oval, elongated oval, and pear-shaped, corresponding to higher values of the Stummer index (lower aspect ratio) for oval, and lower values of
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Seed shape descriptions of species of Vitis have traditionally been based on adjectives comparing overall shape with geometric figures, such as oval, elongated oval, and pear-shaped, corresponding to higher values of the Stummer index (lower aspect ratio) for oval, and lower values of the Stummer index for pear shape (or elongated seeds, with a higher aspect ratio). Analytical, quantitative descriptions of shape have recently been applied to diverse genera of Vitaceae and cultivated varieties of Vitis. Here, we present the application of three quantitative methods to the seed shape description of ten species of the genus Vitis and three species of related genera (Ampelopsis, Cissus and Parthenocissus). First, general seed shape was described through comparisons using geometric models. For this, the average silhouettes of representative seed populations were used as models for shape quantification. Two additional quantitative methods were based on the measurement of bilateral symmetry and curvature analysis in the apex. Quantitative methods for shape description based on similarity with the models give an accurate account of the relationships between Vitis species. The resulting dendrogram is like the dendrogram obtained from a combined analysis using the data from general measurements and curvature and symmetry analyses. The original methods presented here for seed morphology are useful for analyzing the phylogenetic relationships between species of Vitis.
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(This article belongs to the Section Propagation and Seeds)
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Open AccessReview
A Review on Flower Bulb Micropropagation: Challenges and Opportunities
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Sara Yasemin and Margherita Beruto
Horticulturae 2024, 10(3), 284; https://doi.org/10.3390/horticulturae10030284 - 15 Mar 2024
Abstract
This comprehensive review scrutinizes tissue culture and micropropagation methodologies in geophytes, focusing on bulbous plants. The examination encompasses key stages, including somatic embryogenesis, bulb growth, dormancy breaking, and planting. Studies underscore the pivotal role of plant growth regulators (PGRs) in plant regeneration and
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This comprehensive review scrutinizes tissue culture and micropropagation methodologies in geophytes, focusing on bulbous plants. The examination encompasses key stages, including somatic embryogenesis, bulb growth, dormancy breaking, and planting. Studies underscore the pivotal role of plant growth regulators (PGRs) in plant regeneration and bulb growth. Bioreactor systems for healthy plant regeneration, rooting methods, acclimatization strategies, and considerations for ex vitro survival are elucidated. The review also delves into somaclonal variation dynamics and acknowledges the burgeoning field of gene editing, particularly Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) studies, as a promising avenue for enhancing valuable compound content in geophytes. In addition to addressing challenges in flower bulb micropropagation, this review briefly highlights emerging opportunities, including the potential integration of artificial intelligence (AI) to optimize culture conditions, predict growth parameters, and enhance efficiency in bulb production. The conclusion emphasizes the necessity of a multifaceted approach integrating biochemistry, physiology, and molecular biology to address existing challenges and improve tissue culture protocols for diverse geophyte species. This review article also intends to highlight how tissue culture techniques could contribute to the development and valorization of flower bulbs in today’s scenario of the ornamental industry.
Full article
(This article belongs to the Special Issue Advancement in Propagation, Breeding, Cultivation and Marketing of Ornamentals)
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Open AccessArticle
Organogenesis of Plant Tissues in Colchicine Allows Selecting in Field Trial Blueberry (Vaccinium spp. cv Duke) Clones with Commercial Potential
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Ricardo Hernández, Alan López, Bárbara Valenzuela, Vivian D’Afonseca, Aleydis Gomez and Ariel D. Arencibia
Horticulturae 2024, 10(3), 283; https://doi.org/10.3390/horticulturae10030283 - 15 Mar 2024
Abstract
Plants’ genetic improvement continues to be crucial for modern agriculture, while biotechnology can offer efficient tools that enhance the selection and recommendation processes of elite clones. This work established a suitable methodology for the regeneration of blueberry (Vaccinium corymbsum) plants in
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Plants’ genetic improvement continues to be crucial for modern agriculture, while biotechnology can offer efficient tools that enhance the selection and recommendation processes of elite clones. This work established a suitable methodology for the regeneration of blueberry (Vaccinium corymbsum) plants in cultures with colchicine. This could be considered a basis for producing populations for the selection of clones following a genetic improvement program assisted by biotechnology. The factors studied were: (a) explant type (leaf discs; nodal segments); (b) colchicine concentration (0, 0.5, 1, and 2 mg/L); and (c) time of exposure to colchicine (1, 2, 3, 5, and 30 days). The basal medium McCown’s Woody Plant (WP) supplemented with 2 mg/L 2iP and 1 mg/L BAP was used with the commercial genotype Duke as a model. A total of 1957 blueberry clones were produced in a medium with 1 mg/L colchicine, distributed at different exposure times. Flow cytometry analyses revealed the following patterns: single patterns for random samples of control plants (Duke donor) and some clones regenerated on colchicine; double patterns for chlorotic plants regenerated on colchicine. Triple and quadruple patterns were observed in callus tissues that did not regenerate plants on colchicine. Populations of plants regenerated in colchicine (6787) and control plants regenerated in in vitro culture without colchicine were adapted under greenhouse conditions. The variables evaluated at this stage were adaptability, height, diameter, number of leaves, incidence of diseases, flowering capacity, and agrobotanical traits. Selected clones demonstrating phenotypic variability (157 clones) were transplanted to field conditions. From the clonal field trial conducted under minimum tillage conditions, 38 clones were selected for improved traits related to the agricultural yield and nutritional quality of the fruits. Of these, six clones showed the highest agronomic performance and adaptability to adverse environmental conditions compared to the Duke donor genotype. It is recommended that these clones continue genotype × environment interaction trials at different locations.
Full article
(This article belongs to the Special Issue Advances in Berry Crops Production, Genomics and Breeding)
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Open AccessArticle
Genome-Wide Prediction and Expression Characterization of the GATA Gene Family under Nitrogen and Phosphate Deficiency in Panax ginseng
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Hao Liang, Hai Sun, Cai Shao, Jiaqi Qian, Bochen Lv, Jiapeng Zhu and Yayu Zhang
Horticulturae 2024, 10(3), 282; https://doi.org/10.3390/horticulturae10030282 - 15 Mar 2024
Abstract
GATA transcription factors are widespread in plants, exerting crucial functions in multiple processes such as flower development, photoperiod regulation, and light signal transduction. The GATA gene family has a key role in the regulation of medicinal plant adaptation to environmental stress. However, since
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GATA transcription factors are widespread in plants, exerting crucial functions in multiple processes such as flower development, photoperiod regulation, and light signal transduction. The GATA gene family has a key role in the regulation of medicinal plant adaptation to environmental stress. However, since the publication of the Ginseng (Panax ginseng C.A. Meyer) genome-wide data, there has never been an analysis of the whole GATA gene family. To understand the function of the GATA gene family more broadly, the GATA gene family members in P. ginseng were predicted using an in silico bioinformatics approach. A comprehensive and systematic analysis encompassing chromosome scaffold, expression pattern, gene structure, and phylogeny was conducted. The results showed that a total of 52 GATA gene family members were recognized in P. ginseng, distributed across 51 scaffolds. Each member encoded a diverse number of amino acid residues, extending from 138 to 1064. Moreover, the expression levels of PgGATA genes were significantly altered by nitrogen (N) and phosphorus (P) stresses. The expression levels of PgGATA6, PgGATA11, PgGATA27, PgGATA32, PgGATA37, PgGATA39, PgGATA40, and PgGATA50 exhibited significant elevation under N deficiency, whereas PgGATA15, PgGATA18, PgGATA34, PgGATA38, PgGATA41, and PgGATA44 genes showed substantial upregulation under P deficiency. In addition, PgGATA3, PgGATA4, PgGATA14, PgGATA19, and PgGATA28 were substantially upregulated under both N and P deficiency. This research establishes a theoretical foundation for the thorough examination of the functions of the PgGATA gene family and its regulation by N and P fertilization during P. ginseng cultivation.
Full article
(This article belongs to the Section Genetics, Genomics, Breeding, and Biotechnology (G2B2))
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Open AccessReview
Emerging Perspectives on Non-Chemical Weed Management Tactics in Container Ornamental Production in the United States
by
Greeshmanth Alluri and Debalina Saha
Horticulturae 2024, 10(3), 281; https://doi.org/10.3390/horticulturae10030281 - 15 Mar 2024
Abstract
Weeds are undesirable plants that can interfere with human activities and can hamper crop production and practices. The competition among ornamentals and weeds for space, nutrition, light, and moisture within a restricted area, such as in container production, can be intense and destructive.
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Weeds are undesirable plants that can interfere with human activities and can hamper crop production and practices. The competition among ornamentals and weeds for space, nutrition, light, and moisture within a restricted area, such as in container production, can be intense and destructive. In response to increasing concerns regarding herbicide injuries and the effects of pesticide use on the environment, many growers are extremely interested in non-chemical pest-management approaches. There are various non-chemical strategies to control weeds in containers, which include scouting, sanitation practices, hand weeding, mulching, irrigation management, substrate stratification, mulch discs or geo discs, lid bags, and fertilizer placement. In a restricted growth environment, weeds have been shown to reduce crop growth significantly. Limited information is available on the effects of weed densities and container sizes on ornament–weed competition within containerized production and how the concepts of fertilizer placement can be used efficiently to control weeds in containers without using any herbicides on the ornamentals. There is an immediate need to evaluate the interference and competitive effects of pernicious weed species in container-grown ornamentals in the North Central United States and to develop effective non-chemical weed control strategies by altering fertilizer placement in container production.
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(This article belongs to the Section Floriculture, Nursery and Landscape, and Turf)
Open AccessEditorial
Medicinal, Aromatic, and Spice Plants: Biodiversity, Phytochemistry, Bioactivity, and Their Processing Innovation
by
Ivan Salamon
Horticulturae 2024, 10(3), 280; https://doi.org/10.3390/horticulturae10030280 - 15 Mar 2024
Abstract
For centuries, drugs were entirely of natural origin, composed of herbs, animal products, and inorganic materials [...]
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(This article belongs to the Special Issue Medicinal, Aromatic, Spice Plants: Biodiversity, Phytochemistry, Bioactivity and Their Processing Innovation)
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Open AccessFeature PaperArticle
Physical Properties and Crop Performance of Four Substrate Fibers in Greenhouse Petunia Production
by
Maureen E. Thiessen, Jeb S. Fields and Damon E. Abdi
Horticulturae 2024, 10(3), 279; https://doi.org/10.3390/horticulturae10030279 - 15 Mar 2024
Abstract
As peat (P) demand increases throughout the horticultural industry, alternative fibers must be evaluated. Sugarcane bagasse (B), wood fiber (W), and coconut coir (C) have received interest as domestically available alternatives to P, with demonstrated success in producing greenhouse crops. However, there is
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As peat (P) demand increases throughout the horticultural industry, alternative fibers must be evaluated. Sugarcane bagasse (B), wood fiber (W), and coconut coir (C) have received interest as domestically available alternatives to P, with demonstrated success in producing greenhouse crops. However, there is limited research comparing these materials to peat. This research evaluated the substrate properties and productivity of Petunia Supertunia Mini Vista ‘Indigo’ in pine bark substrates amended with C, W, B, or P and fertigated weekly at 100, 200, or 300 parts per million (ppm) nitrogen (N) to account for possible N immobilization. The container capacity was lowest and air-filled porosity was highest in W and B substrates. Substrate pH increased in W and B substrates, and C substrates were fertigated at 100 ppm N. Increasing the N rate increased the growth index in all substrates, especially B and W substrates later in the production period. Higher fertilization increased shoot mass, chlorophyll content, and blooms across all substrates, demonstrating that fertilizer supplementation may offset possible N immobilization. While plant growth and quality parameters were greatest in the P blend, increasing N applications produced similar-quality plants using alternative substrates, demonstrating that modifying fertilizer management practices can make alternative fibers a viable horticultural substrate.
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(This article belongs to the Special Issue Amendments in Soilless Culture for Improved Productivity of Horticultural Plants)
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Open AccessArticle
An Evaluation of Insecticidal Trunk Injections for the Control of the European Cherry Fruit Fly Rhagoletis cerasi L. (Diptera: Tephritidae)
by
Rita Gyuris, Árpád Szabó, Anna M. László, Ádám Gutermuth and Csilla Sörös
Horticulturae 2024, 10(3), 278; https://doi.org/10.3390/horticulturae10030278 - 14 Mar 2024
Abstract
Cherry productivity is challenging in Europe due to the presence of the cherry fruit fly (Rhagoletis cerasi). Trunk injection is an alternative method of insecticide delivery that may improve pesticide performance in controlling pests. In our study, four pesticides (abamectin, acetamiprid,
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Cherry productivity is challenging in Europe due to the presence of the cherry fruit fly (Rhagoletis cerasi). Trunk injection is an alternative method of insecticide delivery that may improve pesticide performance in controlling pests. In our study, four pesticides (abamectin, acetamiprid, flupyradifurone, and cyantraniliprole) were investigated for trunk injection against R. cerasi. Acetamiprid trunk injection in a minimum dose of 0.56 g/tree was suitable for protection against the pest, the efficacy of the control was over 95%, and the pesticide residue concentrations in the fruits were below the maximum permissible limit in each experiment. The trunk injection and foliar spray of the same dose of acetamiprid were equally effective. In the case of the other three active ingredients (abamectin, flupyradifurone, and cyantraniliprole), the trunk injection method did not prove suitable for practical use due to various reasons, which are detailed in the manuscript.
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(This article belongs to the Section Insect Pest Management)
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Open AccessArticle
Pull-Out Resistance of Rebar Stake Depending on Installation Conditions and Compaction Levels of Agricultural Soil
by
Giseok Heo, Inhyeok Choi, Jinyoung Lee, Heedu Lee, Seongyoon Lim and Dongyoup Kwak
Horticulturae 2024, 10(3), 277; https://doi.org/10.3390/horticulturae10030277 - 13 Mar 2024
Abstract
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Strong winds, particularly in the absence of disaster-resistant designs, significantly impact the stability of greenhouse foundations and eventually lead to structural damage and potential harm to crops. As a countermeasure, rebar stakes are commonly used to reinforce the foundations of non-disaster-resistant greenhouses. This
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Strong winds, particularly in the absence of disaster-resistant designs, significantly impact the stability of greenhouse foundations and eventually lead to structural damage and potential harm to crops. As a countermeasure, rebar stakes are commonly used to reinforce the foundations of non-disaster-resistant greenhouses. This study evaluates the pull-out resistance (Rpull-out) of rebar stakes considering various factors like soil compaction, embedded length, installation duration and angle, and changes in soil water content against uplift pressure by strong winds. A combination of field (i.e., the cone penetration test and rebar stake pull-out test) and laboratory (i.e., the compaction test, soil compaction meter test, and soil box test) tests are performed for the assessment of Rpull-out. The results indicate that Rpull-out increases with higher soil compaction, greater embedded length, longer installation duration, and an inclined installation angle. The soil compaction exerts the most significant impact; 90% to 100% of the soil compaction rate has approximately 10 folds higher Rpull-out than the 60–70% compaction rate. If the embedded length is increased from 20 cm to 40 cm, there is a two-fold increase in the average of Rpull-out. Inclined installation of rebar stakes increases Rpull-out by 250% to 350% compared to vertical installation, and rebar stakes installed prior to the uplift event have 1.5 to 6.4 fold increases in Rpull-out than those with instant installation. Additionally, we observed variations in the surface soil moisture due to climatic changes introducing variability in Rpull-out. These findings lead to the proposition of efficient rebar stake installation methods, contributing to the enhanced stability of a greenhouse.
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Open AccessArticle
A MYB Transcription Factor from Brassica juncea Regulates Purple Leaves in Pak Choi (Brassica campestris L. ssp. chinensis)
by
Xia Wang, Yating Zhu, Bo Shi, Shujiang Zhang, Shifan Zhang, Hui Zhang, Rifei Sun, Jinyan Zhou, Ze Li, Guoliang Li and Fei Li
Horticulturae 2024, 10(3), 276; https://doi.org/10.3390/horticulturae10030276 - 13 Mar 2024
Abstract
The purple-leaf phenotype in pak choi is due to the accumulation of anthocyanin. The main regulated genes are unclear. The gene controlling the purple-leaf phenotype was mapped on A03 using BSA-seq, but the candidate interval was not further narrowed with InDel markers. Based
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The purple-leaf phenotype in pak choi is due to the accumulation of anthocyanin. The main regulated genes are unclear. The gene controlling the purple-leaf phenotype was mapped on A03 using BSA-seq, but the candidate interval was not further narrowed with InDel markers. Based on our previous study, we hypothesized that the candidate gene that regulates purple leaves in pak choi may also be the Dark_Pur gene from B. juncea. Using the Dark_Pur-1 marker to identify P1, P2, F1, and F2, it was confirmed that the purple trait in purple-leaf pak choi was controlled by the Dark_Pur gene from B. juncea through distant hybridization. A DNA segment of approximately 514 Kb containing the Dark_Pur gene was reintroduced into pak choi from B. juncea. Meanwhile, a new purple pak choi germplasm line was created with green pak choi × purple B. juncea via distant hybridization, which proved that distant hybridization is an effective method for creating new germplasms. Furthermore, the purple-leaf phenotypes of 20 pak choi varieties were identified, and the purple-leaf traits of all lines were derived from B. juncea via distant hybridization. At present, few studies have focused on the background of the purple trait in pak choi; however, in this study, our results suggest that there is a high probability that the purple trait in pak choi may be completely derived from purple B. juncea via distant hybridization. This study also lays a good foundation for research on the creation of new germplasms through distant hybridization among the Brassica species.
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(This article belongs to the Section Genetics, Genomics, Breeding, and Biotechnology (G2B2))
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Open AccessArticle
Study on Target Detection Method of Walnuts during Oil Conversion Period
by
Xiahui Fu, Juxia Wang, Fengzi Zhang, Weizheng Pan, Yu Zhang and Fu Zhao
Horticulturae 2024, 10(3), 275; https://doi.org/10.3390/horticulturae10030275 - 12 Mar 2024
Abstract
The colors of walnut fruits and leaves are similar in the oil transformation period, and the fruits are easily blocked by the branches and leaves. On the basis of the improved YOLOv7-tiny, a detection model is proposed and integrated into an Android application
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The colors of walnut fruits and leaves are similar in the oil transformation period, and the fruits are easily blocked by the branches and leaves. On the basis of the improved YOLOv7-tiny, a detection model is proposed and integrated into an Android application to solve the problem of walnut identification. Ablation experiments conducted with three improved strategies show that the strategies can effectively enhance the performance of the model. In terms of combinatorial optimization, the YOLOv7-tiny detection model that combines FasterNet and LightMLP modules works excellently. Its AP50 and AP50–95 are 3.1 and 4 percentage points (97.4% and 77.3%, respectively) higher than those of the original model. YOLOv7-tiny’s model size and number of parameters are reduced by 14.6% and 14.4%, respectively, relative to those of the original model, and its detection time decreases to 15.4 ms. The model has good robustness and generalization ability and can provide a technical reference for intelligent real-time detection of walnuts during the oil conversion period.
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(This article belongs to the Special Issue Advances in Intelligent Orchard)
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