Topic Editors

Key Laboratory of Integrated Rice-Fish Farming, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, China
Dr. Zhihui Sun
Key Laboratory of Mariculture & Stock Enhancement in the North China Sea, Ministry of Agriculture and Rural Affairs, Dalian Ocean University, Dalian 116023, China
Dr. Jun Zhang
Engineering Technology Research Center of Healthy Aquaculture, Anhui Agricultural University, Hefei 230036, China

Sex Differentiation Mechanisms in Aquatic Species

Abstract submission deadline
31 October 2026
Manuscript submission deadline
31 January 2027
Viewed by
2284

Topic Information

Dear Colleagues,

Sex differentiation is a fundamental biological process that determines whether an organism develops as a male, female, or hermaphrodite. Pronounced sexual dimorphism in growth rate, body size, and gonadal development is frequently observed across aquatic species. Furthermore, the strong market demand for the mature gonads of species such as sturgeon, crabs, and sea urchins makes controlling the sex of these organisms an important breeding objective in aquaculture. Therefore, understanding the mechanisms of sex determination and differentiation is essential not only for developmental and evolutionary biology, but also sex-control technologies that can improve aquaculture productivity and efficiency.

Rapid progress in high-throughput sequencing, genome editing, and multi-omics technologies has greatly accelerated research on key regulatory genes, epigenetic pathways, endocrine signals, and environmental factors involved in sex differentiation. These advances have deepened our understanding of the molecular and cellular foundations of sexual development in various aquatic species and enabled applications such as sex-controlled breeding, germ cell manipulation, and sustainable aquaculture production.

This Special Issue welcomes studies that provide new insights into the molecular, cellular, developmental, and environmental mechanisms underlying sex differentiation in aquatic species, including both vertebrates and invertebrates. Contributions employing genomics, transcriptomics, epigenetics, cell culture, functional assays, comparative analyses, or innovative breeding strategies are particularly encouraged.

Prof. Dr. Mingyou Li
Dr. Zhihui Sun
Dr. Jun Zhang
Topic Editors

Keywords

  • sex determination and sex differentiation
  • germ cell and gonad development
  • vertebrate and invertebrate reproductive biology
  • environmental influences on sex differentiation
  • sex-controlled and unisexual breeding in aquaculture
  • germ cell manipulation, transplantation, and surrogate reproduction

Participating Journals

Journal Name Impact Factor CiteScore Launched Year First Decision (median) APC
Animals
animals
3.2 5.5 2011 15.7 Days CHF 2400 Submit
Aquaculture Journal
aquacj
- 4.0 2021 18.8 Days CHF 1200 Submit
Biology
biology
4.3 7.3 2012 14.2 Days CHF 2700 Submit
Fishes
fishes
2.9 4.4 2016 18.2 Days CHF 2600 Submit
Life
life
3.9 7.1 2011 15.3 Days CHF 2600 Submit

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Published Papers (1 paper)

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15 pages, 9202 KB  
Article
Molecular Insights into Sex Differentiation of Rhinogobio nasutus via Integrated mRNA and miRNA Profiling
by Jie Yin, Yanbin Liu, Muhammad Jawad, Haijing Xu, Muyan Li, Zongqiang Lian and Mingyou Li
Fishes 2026, 11(6), 342; https://doi.org/10.3390/fishes11060342 - 8 Jun 2026
Viewed by 630
Abstract
Rhinogobio nasutus, an endangered fish species endemic to the upper and middle reaches of the Yellow River in China, lacks essential genomic information on gonadal development, hindering research into its reproductive biology. To address this, mRNA-seq and miRNA-seq datasets derived from adult [...] Read more.
Rhinogobio nasutus, an endangered fish species endemic to the upper and middle reaches of the Yellow River in China, lacks essential genomic information on gonadal development, hindering research into its reproductive biology. To address this, mRNA-seq and miRNA-seq datasets derived from adult testis (n = 3) and ovary (n = 3) were integrated to characterize sex-biased expression profiles and potential regulatory mechanisms. A total of 34,813 genes and 68,623 transcripts were detected, and 16,105 differentially expressed genes (DEGs) were identified between testis and ovary, including 9365 testis-biased and 6740 ovary-biased genes. Small-RNA profiling identified 51 differentially expressed miRNAs (DEMs: 31 testis-biased; 20 ovary-biased). The sex-biased mRNA profiles highlighted conserved candidate genes associated with germ-cell maintenance, somatic regulation, ovarian differentiation, and oocyte maturation, including vasa, piwi, dmrt1, amh, cyp19a1a, zar1, zar1l, and rbpms2. Integrated miRNA–mRNA analysis further predicted potential interactions involving key sex-related genes, suggesting that DEMs may contribute to post-transcriptional regulation during gonadal differentiation. Functional enrichment (GO and KEGG analyses) highlighted pathways associated with gonadal differentiation, germline maintenance, and signal transduction pathways. qRT-PCR validation of nine mRNAs and nine miRNAs showed expression patterns consistent with the sequencing results. Collectively, these results provide an integrated mRNA and miRNA resource for R. nasutus gonads and identify candidate genes and miRNAs for future studies on sex-biased gonadal development, reproductive regulation, and artificial propagation. Full article
(This article belongs to the Topic Sex Differentiation Mechanisms in Aquatic Species)
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