Topic Editors

Department of Molecular Medicine and Medical Biotechnology, University of Naples "Federico II", Via S. Pansini 5, 80131 Naples, Italy
Department of Molecular Medicine and Medical Biotechnology, School of Medicine, University of Naples Federico II, 80131 Naples, Italy

Liquid Biopsy: A Modern Method Transforming Biomedicine

Abstract submission deadline
31 December 2025
Manuscript submission deadline
31 March 2026
Viewed by
543

Topic Information

Dear Colleagues,

Advancements in the understanding of circulating molecular and cellular components across various human bodily fluids have paved the way for liquid biopsy (LB) to emerge as a transformative tool in biomedical research. Whether liquid or solid, the fundamental purpose of a biopsy remains the same: to guide clinical intervention. As a non-invasive alternative to traditional surgical tissue removal, LB serves as a dynamic and real-time surrogate, reflecting the physiological and pathological status of its tissue of origin. Moreover, there is now, in fact, strong concordance between molecular alterations detected in liquid biopsies and their corresponding tissue biopsies.

Defined by its ability to provide a readily accessible snapshot of parental cells or tissues, LB encompasses a wide range of analytes, including circulating tumor cells, cell-free DNA and RNA, extracellular vesicles, proteins, and metabolites. All of these molecules can be isolated from diverse bodily fluids, including blood, urine, saliva, cerebrospinal fluid, and more. By offering a minimally invasive approach, liquid biopsy enables the investigation of key pathological processes such as disease onset, progression, treatment response, and resistance through simple and accessible tests. This capability aligns seamlessly with the rise of personalized medicine, where -omics studies are leveraged to identify novel diagnostic markers, elucidate disease mechanisms, and uncover therapeutic targets, further enhancing its clinical potential.

As part of this topic, we aim to provide a comprehensive overview of the evolving field of liquid biopsy in biomedicine, highlighting cutting-edge research and clinical applications. Special emphasis will be placed on a broad spectrum of omics studies, including but not limited to proteogenomics, lipidomics, fluxomics, data analysis, bioinformatics, single-cell omics, and post-translational modification (PTM) characterization. Additionally, we welcome contributions focusing on the development of novel strategies for the isolation and characterization of biomolecules from LB, in addition to advancements in data analysis and computational methodologies. These insights will help expand knowledge and engage a broader scientific audience.

A key focus will be identifying the challenges associated with integrating LB into clinical trials and routine practice, ensuring a multidimensional perspective. Overcoming these barriers is crucial to establishing LB as a standard diagnostic tool, ultimately making precision medicine a tangible reality for patients worldwide.

Dr. Michele Costanzo
Dr. Marianna Caterino
Topic Editors

Keywords

  • liquid biopsy
  • mass spectrometry
  • biomarker discovery
  • extracellular vesicles
  • proteomics
  • metabolomics
  • lipidomics
  • single-cell omics
  • integrated omics

Participating Journals

Journal Name Impact Factor CiteScore Launched Year First Decision (median) APC
International Journal of Molecular Sciences
ijms
4.9 8.1 2000 16.8 Days CHF 2900 Submit
Metabolites
metabolites
3.5 5.7 2011 16.1 Days CHF 2700 Submit
Molecules
molecules
4.2 7.4 1996 15.1 Days CHF 2700 Submit
Proteomes
proteomes
4.0 6.5 2013 27.3 Days CHF 1800 Submit
Biomedicines
biomedicines
3.9 5.2 2013 14.6 Days CHF 2600 Submit
International Journal of Translational Medicine
ijtm
- - 2021 24.2 Days CHF 1000 Submit

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Published Papers (1 paper)

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31 pages, 3339 KiB  
Review
Liquid Biopsy in Pituitary Neuroendocrine Tumors—Potential Biomarkers for Diagnosis, Prognosis, and Therapy
by Ligia Gabriela Tataranu
Int. J. Mol. Sci. 2025, 26(9), 4058; https://doi.org/10.3390/ijms26094058 - 25 Apr 2025
Viewed by 382
Abstract
Pituitary neuroendocrine tumors (PitNETs) are slow-growing neoplasms with various clinical presentations, often leading to diagnostic challenges. While neuroimaging assessment and histopathological evaluation remain the gold standard for diagnosis, emerging research highlights the potential of liquid biopsy, mainly through the analysis of circulating non-coding [...] Read more.
Pituitary neuroendocrine tumors (PitNETs) are slow-growing neoplasms with various clinical presentations, often leading to diagnostic challenges. While neuroimaging assessment and histopathological evaluation remain the gold standard for diagnosis, emerging research highlights the potential of liquid biopsy, mainly through the analysis of circulating non-coding RNAs (ncRNAs), as a promising diagnostic and prognostic tool. Recent studies have demonstrated distinct expression profiles in different types and subtypes of tumors, with implications in assessing tumor aggressiveness and predicting treatment response. The current article summarizes data about potential biofluid markers implicated in PitNET development, mainly circulating tumor DNA (ctDNA), cell-free RNAs (cfRNA), circulating tumor cells (CTCs), and exosomes. Many studies on genetic and molecular markers in PitNET tissue samples provide exciting information about tumor biology, but to date, specific studies on liquid biopsy biomarkers are still few. Over the past years, a certain understanding of the mechanisms involved in pituitary tumorigenesis has been gained, including the landscape of genomic alterations, changes in epigenetic profile, crucial molecules involved in specific signaling pathways, and non-coding RNA molecules with critical roles in malignant transformation. Genetic and molecular characterization of the PitNETs could help distinguish between functional and non-functional PitNETs, different types and subtypes of pituitary tumors, and invasive and non-invasive forms. Further studies are required to identify and validate innovative biomarkers or therapeutic targets for treating PitNET. Integrating liquid biopsy into clinical workflows could revolutionize the management of pituitary adenomas, enabling more personalized and less invasive diagnostic and therapeutic strategies. Full article
(This article belongs to the Topic Liquid Biopsy: A Modern Method Transforming Biomedicine)
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