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Background: Inadequate water intake is prevalent among Chinese college students, a group at a critical stage for establishing lifelong health habits. However, nationwide data on their knowledge, attitudes, and practices (KAP) regarding water intake remain scarce. This study aims to describe regional variations in water-related KAP among undergraduates across seven major geographical regions of China, providing evidence for developing targeted health promotion strategies. Methods: A cross-sectional survey employed multistage stratified convenience sampling to recruit undergraduate students (N = 3161) from one university in each of China’s seven regions. Participants completed a KAP questionnaire. Data analysis utilized chi-square tests with Bonferroni correction, reporting effect sizes and confidence intervals. Results: A total of 3161 valid responses were obtained (response rate: 98.3%). Students in South China demonstrated the lowest awareness of regular water intake (52.0%) but the highest awareness of daily recommended water intake (32.9%). South China and Northeast China exhibited weaker recognition of water’s importance (65.6% and 94.0%, respectively) and the lowest prevalence of “thirst-driven” drinking behavior (21.7% and 32.4%, respectively). Conclusions: The knowledge, attitude, and practice (KAP) status regarding water consumption among Chinese university students is concerning and exhibits significant regional disparities. Key issues include knowledge gaps, disconnect between attitudes and behaviors, and deeply ingrained unscientific drinking habits. Analysis based on KAP theory indicates that future health promotion strategies must move beyond mere knowledge dissemination and adopt region-specific, multilevel comprehensive interventions. Full article

Smart cities aim to integrate technological, infrastructural, and socio-environmental systems in order to improve urban sustainability and quality of life. To qualify as both smart and sustainable, a city is generally expected to pursue self-sufficiency through the adoption of sustainable practices in energy production, water supply, and food systems. Such cities also seek to reduce operational costs for both private operators and municipalities, while aiming to enhance the quality of life of their residents. Within this context, the relevance of a web-based application becomes particularly apparent. An application equipped with predefined indicators can provide a structured and measurable framework for assessing the current status of a city or town in relation to smart and sustainable development. This framework allows for the evaluation of the extent to which a city aligns with established criteria associated with smart and sustainable urban models. This paper introduces a Python-based web application, developed using Python version 3.10, designed to assess or support the self-assessment of a city’s alignment with identified smart and sustainable development indicators. This study does not claim empirical validation or benchmarking performance; the proposed system is presented as a proof-of-concept framework. The work does not propose new smart city indicators. Rather, it presents an integrative system that seeks to operationalise existing smart and sustainable city indicators within a unified and modular web-based assessment framework, designed to support cross-domain evaluation and citizen-accessible self-assessment. Full article

Background: Vegetarian diets are becoming increasingly popular among young adults. The aim of this study was to examine the association between adherence to a vegetarian diet and body composition parameters in young adult women. Methods: A sample of 647 young adult women, mainly university students from Slovakia, consisting of 66 vegetarians (22.02 ± 2.74 years old) and 581 omnivores (21.13 ± 2.20 years old) was analysed. Body composition was measured using a bioelectrical impedance analyser, the InBody 770. Information on participants’ diet was collected using a modified version of the WHO STEPS 2014 questionnaire. Results: Vegetarian women showed significantly lower phase angle (PhA) values compared with omnivores (5.06 ± 0.45 vs. 5.23 ± 0.48; p = 0.004). They also had a higher extracellular-to-total body water ratio (ECW/TBW; 0.382 ± 0.004 vs. 0.380 ± 0.005; p = 0.026). In multivariable linear regression adjusted for smoking status, physical activity, body mass index (BMI) and waist-to-hip ratio (WHR), vegetarian diet remained independently associated with lower PhA (β = −0.094; p = 0.011) and higher ECW/TBW (β = 0.085; p = 0.028). No significant indirect associations between a vegetarian diet and PhA or ECW/TBW through smoking status or physical activity were observed. Conclusions: In this study of young adult Slovak women, adherence to a vegetarian diet was independently associated with lower PhA and a higher ECW/TBW ratio. These findings indicate differences in BIA-derived indicators of cellular integrity and fluid distribution between vegetarian and omnivorous women, although causal relationships cannot be inferred. Full article

Limnological parameters were monitored in four highland reservoirs in the Czech Republic from 2022 to 2024 to evaluate the effects of management practices on water quality. Although the reservoirs share similar morphometry and all serve as drinking water sources, they differ in trophic status and management: Hubenov (HU, eutrophic) is stocked with piscivores, Nová Říše (NŘ, mesotrophic) undergoes hypolimnetic aeration, and Landštejn (LA, meso-oligotrophic) and Mostiště (MO, eutrophic) receive no targeted management interventions. Limnological data were collected monthly from April to October along vertical profiles in dam parts of the reservoirs. Comparisons were performed using graphical presentation and linear mixed-effects models. Analyses of abiotic (thermal, oxygen, and pH stratification, transparency, total phosphorus (TP) and nitrogen (TN) concentrations) and biotic (algae chlorophyll-a, cyanobacterial pigments, zooplankton density and composition) variables revealed that HU and MO exhibited the lowest transparency (on average 1.9 m in both in contrast to 2.2 m and 2.8 m in NŘ and LA, respectively) and highest seasonal algae chlorophyll-a concentrations (11.4 µg/L in HU and 15.1 µg/L in MO in contrast to 6.4 µg/L in NŘ and 5.5 µg/L in LA), indicating negligible improvement from biomanipulation. In contrast, NŘ demonstrated nutrient and chlorophyll-a levels comparable to LA (TP: 0.010 mg/L and 0.009 mg/L, TN: 1.591 mg/L and 0.419 mg/L, in NŘ and LA, respectively), despite higher nutrient input, and achieved the second highest transparency. Zooplankton densities were similar across reservoirs, supporting the hypothesis of bottom-up control or insufficient piscivore impact. These findings highlight the importance of reducing nutrient inputs to preserve water quality. Hypolimnetic aeration, which enhances sediment nutrient retention, appears more effective at mitigating eutrophication and controlling algal proliferation than fish stocking, a commonly applied biomanipulation approach. Full article

Lake Kotokel is one of the largest lakes along the eastern shore of Lake Baikal. Increasing anthropogenic pressure combined with climate variability led to a sharp decline in its ecological condition, culminating in an outbreak of Gaff disease in 2009. Moreover, Lake Kotokel may serve as a source of pollutants to Lake Baikal due to its hydrological connection via a system of rivers. In light of these factors, ongoing research seeks to identify the drivers of ecological degradation in the lake ecosystem and provide a comprehensive assessment of its current environmental status and potential adverse processes. In this study, we report, for the first time, the occurrence of microplastic particles in the surface water and sediments of Lake Kotokel. Mean microplastic concentrations were 0.59 ± 0.25 items/m³ in water and 280 ± 162 items/kg dry weight in sediments, with corresponding average microplastic masses of 2.6 ± 1.4 μg/m³ in water and 1.33 ± 1.21 mg/kg dry weight in sediments. In surface waters, microplastic were predominantly 1–3 mm in size, whereas sediments were dominated by 0.1–1 mm particles. Fibers were the most abundant morphological form, comprising 79.5% and 82.8% of particles in water and sediments, respectively. Five polymer types were identified, with polyethylene terephthalate being the most prevalent. Based on particle abundance and polymer composition, the ecological risk of microplastic in Lake Kotokel was assessed as low to moderate. Full article

This study aimed to evaluate the stock status of the Japanese flying squid (Todarodes pacificus), a critical fishery resource in the waters of Korea, China, and Japan. To achieve this objective, we employed the Bio-Economic Stock Assessment (BESA) model, which integrates catch and market price data to estimate the biological and economic parameters of Japanese flying squid biomass. The assessment results indicated that the current biomass level of Japanese flying squid is below the biomass at Maximum Sustainable Yield ($B_{\mathrm{MSY}}$), suggesting that the stock is overfished. Moreover, the findings from the BESA model were consistent with results obtained from the Monte Carlo Method (CMSY) and Bayesian State-Space (BSS) models, both of which also indicated a collapsed status. Unlike the CMSY and BSS models, which rely on catch and catch per unit effort (CPUE) data, the BESA model utilizes market price data from National Statistics and the Food and Agriculture Organization (FAO), thereby eliminating the need for CPUE standardization. Consequently, the BESA model presents an alternative framework that complements existing assessment methods and enhances the reliability of fishery stock evaluations through its integrated approach, suggesting its potential applicability to the stock assessment of Japanese flying squid in Korea. Full article

Ecological restoration of abandoned mines with high and steep slopes is challenging due to their steepness, water scarcity, and lack of soil, and restoration effects vary with applied techniques. This study aims to assess the ecological restoration effectiveness of restored steep bare rock slopes in Jiangsu Province. The restoration status of steep bare rock slopes was assessed through field surveys, with corresponding methods for vegetation data collection and soil sample analysis subsequently selected. An evaluation system consisting of 14 evaluation indicators from 3 aspects was established using the analytic hierarchy process (AHP). Based on the on-site investigation results and test data, the evaluation criteria and evaluation intervals for the five evaluation levels of “optimum, excellent, good, medium, and poor” have been determined. After obtaining the weights of each indicator, the Technique for Order Preference by Similarity to an Ideal Solution (TOPSIS) method is used to establish a normalized matrix of evaluation indicators, calculate the weighted decision matrix, and determine the ideal solution. The results showed that (1) the proportion of ecological restoration effects in the excellent–optimum, good–excellent, and medium–good was 30%, 43.3%, and 26.7%, respectively, which is consistent with the on-site investigation results; (2) the on-site investigation and evaluation results indicate that the ecological restoration effect of steep bare rock slopes is easily affected by the slope’s soil and water conservation capacity; (3) the weights of each indicator layer are slope ecosystem stability > vegetation > soil, with a maximum value of 0.443, indicating that ecosystem stability is the main factor affecting the ecological restoration effect of mines. This evaluation system is based on on-site investigations and indoor test results, and objectively and effectively evaluates the ecological restoration effect of steep bare rock slopes through qualitative evaluation and quantitative analysis. The methodology demonstrates high applicability and reliability for steep bare rock slopes, thereby serving as a valuable reference for selecting and evaluating the efficacy of ecological restoration technologies in similar environments. Full article

This research investigates the socioeconomic factors that lead to ineffective plastic waste management in household areas and their consequences for water sustainability in four villages in Guyana: Mon Repos, Lusignan, De Endragt, and Good Hope. The study uses a household survey (N = 384), chi-square tests, and a binary logistic model. It finds that labour status, schooling, and earnings affect unsafe disposal practices like dumping, incineration, or leaving garbage. The risk of inappropriate disposal is 20%, higher than the 6.8% among university graduates. The unemployed are at highest risk, with 32.7% at high risk. Low-income individuals (≤GYD $85,000) have a 21.9% rate, which is 2.5 times higher than the 7.6% rate among higher-income individuals. Strikingly, 63.2% of the high-risk households reported seeing “some” or “vast” amounts of dumped garbage in the close vicinity. It suggests a greater possibility of water contamination. Lacking direct proof of water quality, the strong correlation between socioeconomic disadvantage, litter visibility, and proximity to drainage infrastructure is one of the environmental justice concerns. The findings show plastic littering to be a structural issue associated with inequality, rather than purely individual behaviour, beyond the lack of access to sound waste management services. From a sustainability perspective, there needs to be a priority on straightforward strategies that encompass waste infrastructure development alongside poverty reduction and sustainable management practices. If these intrinsic disparities are not addressed, efforts to protect community water resources and realise SDGs 6, 10, and 12 will likely be futile. Full article

Photocatalytic water sterilization has emerged as a promising sustainable technology for addressing microbial contamination across diverse sectors including healthcare, food production, and environmental management. This review examines the fundamental mechanisms and recent advances in photocatalytic water sterilization, with a particular emphasis on the differential bactericidal pathways against Gram-negative and Gram-positive bacteria. Gram-negative bacteria undergo a two-step inactivation process involving initial outer membrane lipopolysaccharide (LPS) degradation followed by inner membrane disruption, whereas Gram-positive bacteria exhibit simpler kinetics due to direct oxidative attacks on their thick peptidoglycan layer. Escherichia coli has long been used as the gold standard in photocatalytic sterilization studies owing to its aerobic nature and suitability for the colony-counting method. In contrast, Lactobacillus casei, a facultative anaerobe, can be cultured statically and evaluated rapidly using turbidity-based optical density measurements. Therefore, both organisms serve complementary roles depending on the experimental objectives—E. coli for precise quantification and L. casei for rapid, practical assessments of Gram-positive bacterial inactivation under laboratory conditions. We also describe sterilization using light alone while comparing it to photocatalytic sterilization and then discuss two innovative suspension-based photocatalyst systems: polystyrene bead-supported TiO₂/SiO₂ composites offering balanced reactivity and separability and magnetic TiO₂-SiO₂/Fe₃O₄ nanoparticles enabling rapid magnetic recovery. Future research directions should prioritize enhancing visible-light efficiency using metal-doped TiO₂ such as Cu-doped systems; improving catalyst durability; developing new applications of photocatalysts, such as protecting RO membranes; and validating scalability across diverse industrial and medical water treatment applications. Full article

Water deficit is a major constraint limiting the growth and yield of faba bean (Vicia faba L.). A pot experiment was conducted under controlled conditions to evaluate the effect of inoculation with Rhizobium leguminosarum bv. viciae BIHB 1148 (strain F14) and Pseudomonas alkylphenolica PF9 (strain L13) on faba bean drought resilience. Two irrigation regimes were applied: well-watered (80% of field capacity) versus water-stressed (40% of field capacity). Strain F14 was used to ensure effective biological nitrogen fixation, while strain L13 was applied in co-inoculation to evaluate its biostimulatory effects. The control plants received nitrogen in its chemical form. Results indicated that water deficit significantly (p < 0.001) reduced plant growth, nodulation, and photosynthesis-related parameters, and increased hydrogen peroxide (H₂O₂) and malondialdehyde (MDA) levels, which are key markers of oxidative stress. However, co-inoculation with strains F14 and L13 significantly enhanced shoot and root biomass, as well as most agro-morphological traits. It also stimulated (p < 0.05) the antioxidant activities of superoxide dismutase (3-fold), guaiacol peroxidase (12%), and catalase (104%), and increased proline content (119%), which led to lower levels of MDA (54% decrease) and H₂O₂ (55% decrease), improved membrane stability, water status, and enhanced photosynthesis. Overall, co-inoculation of faba bean with Rhizobium leguminosarum bv. viciae BIHB 1148 and Pseudomonas alkylphenolica PF9 offers a promising and sustainable approach to improve plant resilience under water deficit. Full article

Quantitative assessment of ecosystem health (EH) effectively provides a scientific reference for regional landscape ecological development and socio-ecological system coordination. This study combined the VORSH framework and the XGBoost-SHAP model to assess EH and its spatiotemporal driving factors in the Pinglu Canal Economic Zone. The results show that the comprehensive ecosystem health index (EHI) generally remained at a moderate level during this period, exhibiting a pattern of initial decline followed by recovery, resulting in an overall improving trend. The period from 2005 to 2010 was identified as a critical transitional phase, during which EH began to recover and gradually improve. The Pinglu Canal Economic Zone exhibits distinct spatial heterogeneity in EH. Areas with poor and unhealthy grades are primarily distributed around urban peripheries, plain regions, and near certain water bodies. In contrast, healthy and relatively healthy areas are predominantly located in the densely vegetated mountainous regions of the southwest, north, and east. Between 2000 and 2020, the EH status demonstrated a significant overall upward trend, with most areas experiencing slight improvement and only a few regions exhibiting significant degradation. Topography and temperature were the primary factors driving the spatiotemporal variations in EH, while the influence of human activities continued to intensify with ongoing socioeconomic development. Full article

In recent decades, rapid socioeconomic development and population growth have led to the degradation of river and lake health worldwide, posing severe challenges to watershed ecological management. The growing intensity of land-use has significantly contributed to the accelerated deterioration of aquatic ecosystems. River and lake health assessment has evolved from single-parameter metrics (e.g., water quality) to multidimensional frameworks integrating hydrological, biological, and anthropogenic factors. This research conducted a bibliometric analysis of 1302 publications from 1996 to 2023 in the Web of Science database to identify research trends and hotspots. Results showed that publications exhibited a three-phase growth incubation (1996–2000), expansion (2001–2012), and acceleration (2013–2023), with the U.S., China, and Australia as leading contributors characterized by regionally clustered international collaborations. Research themes have shifted from single water quality parameters to integrated assessments. “Land-use”, “water quality”, and “biotic integrity” have emerged as core hotspots, forming a synergistic assessment framework that combines physicochemical, biological, and socioeconomic factors. The research scale underwent a spatial refinement process from the whole watershed to the buffer zone of rivers and lakes, and land-use effects on aquatic ecosystems vary significantly across spatial scales (entire watershed and riparian zones). Fine-scale studies better capture localized pollution pathways, supporting targeted conservation strategies. This review systematically outlines research status, hotspots, and development directions for river and lake health studies, highlighting the need for integrated watershed management, emphasizing conservation through fine-scale land-use monitoring, and providing scientific support for integrated refined governance of watershed ecology. Full article

The ecological status of lakes based on ichthyofauna, as defined by the Water Framework Directive, is assessed using intercalibrated methods. However, the methods adopted (in Poland, the Lake Fish Index LFI-EN method, based on results of one-off fishing with multi-mesh gillnets) are labor-intensive and do not allow for frequent repeat testing. Therefore, the concept of a simple model describing changes in the relative number of single traces in the vertical profile (according to the TS target strength distribution) in a lake is presented, as well as an index (the sum of deviations from such a model), enabling quantification of the similarity of TS distributions in lakes with this model. Preliminary analyses were conducted on acoustic data collected in Lake Dejguny. This lake—the condition of which could be estimated based on historical data using the relationships between LFI and the degree of lake eutrophication (expressed by Carlson’s TSI)—was assessed as having a good status in 2006, whereas in 2021, (based on LFI-EN) it had a moderate status. The study tested the TS distribution model, calculated as the arithmetic mean of the relative number of single traces in 2 m-thick layers. It was also shown that the proposed indicator can effectively signal deterioration of ecological status—the sum of the absolute values of the TS distribution deviations in 2021 (moderate status) from the model was more than seven times greater than the sum of the deviations of the distributions from which the model was built (good status). The obtained results confirmed the hypothesis about the possibility of determining a characteristic distribution of single traces in the vertical profile when the lake was classified as being in good condition. Full article

Increasing aridity and climate extremes are challenging the resilience of key Mediterranean species. Proxies that indicate plant water status, physiological condition and soil water availability are valuable tools for management planning. However, their reliability requires species-specific validation under dynamic environmental conditions. This study examined the relationship between predawn leaf water potential (Ψ_PD) and soil water potential (Ψ_S) in potted seedlings of two co-occurring Mediterranean evergreen oaks, Q. ilex and Q. suber, subjected to imposed soil drying under greenhouse conditions. We further quantified the occurrence and magnitude of predawn disequilibrium (PDD)—the mismatch between Ψ_PD and Ψ_S—and evaluated its association with soil water availability, plant water-status indicators, environmental factors, and physiological variables. In parallel, we assessed stomatal closure dynamics during the desiccation phase and characterised species-specific mortality patterns under progressive drought. Linear Mixed-Effects Models (LMMs), with pot identity included as a random factor, were fitted to assess the relationship between Ψ_PD and Ψ_S, as well as the occurrence of PDD and its potential drivers for each species. Stomatal conductance (g_s) responses to Ψ_S were evaluated using a paired t-test and an additional LMM. Finally, Generalised Linear Mixed-Effects Models (GLMMs) were used to analyse interspecific differences in mortality. We confirmed a tight relationship between Ψ_PD and Ψ_S, followed by a consistent PDD in both species, with magnitudes of 0.53 MPa for Q. ilex and 0.98 MPa for Q. suber, which increased significantly with drought severity. Our findings suggest that PDD under the studied conditions is primarily driven by soil water depletion and plant desiccation, as indicated by its negative correlation with water status parameters, as well as by its increase with progressive drought. Both oaks exhibited a water-saving strategy, with stomatal closure initiated around Ψ_S = −0.31 MPa (Q. ilex) and −0.42 MPa (Q. suber). Despite their physiological similarities, Q. suber showed higher mortality under imposed drought. These results encourage modelling the relationship between Ψ_PD and Ψ_S to accurately interpret plant and soil water needs in Mediterranean oaks, especially under soil water scarcity, and highlight species-specific responses critical for forest management and restoration under climate change. Full article

This study presents the first comprehensive assessment of microalgal diversity in two Slovenian transitional waters (TWs): the shallow brackish lagoon of the Škocjanski Zatok Nature Reserve (SZNR) and the Rižana River estuary within the Port of Koper (PK) area. Between 2018 and 2021, water samples collected with a phytoplankton net were analyzed using light and scanning electron microscopy. In total, 240 species from 117 genera were identified in TW, dominated by diatoms and dinoflagellates, surpassing the diversity at a marine coastal station (91 species, 59 genera). Species richness was higher in PK (226) than in SZNR (154), mainly due to dinoflagellates and coccolithophores. Marine taxa predominated along the salinity gradient, with moderate contributions from brackish taxa and few freshwater forms, reflecting both natural and anthropogenic influences. Planktonic taxa dominated at all sites, while benthic forms were abundant in the lagoon, particularly in spring. Thirty-two taxa were recorded for the first time in Slovenian TW, mostly benthic or tychopelagic diatoms. The detection of Coolia monotis and five cyanobacterial genera with potentially harmful traits highlights the role of TW as an ecological interface. The taxonomic sufficiency analysis showed that the order level is sufficient to distinguish transitional from marine assemblages, beyond which ecological information is lost. Overall, this study highlights the importance of detailed taxonomic resolution for detecting microalgal diversity, including harmful and non-indigenous species to ensure robust ecological assessments under the WFD and MSFD directives. Full article