Search Results (998)

Leptospirosis is a globally significant zoonosis affecting animal health, productivity, and the environment. While typically associated with tropical climates, its persistence in semi-arid regions such as La Laguna, Mexico—characterized by low humidity, high temperatures, and limited water sources—remains poorly understood. Although these adverse environmental conditions theoretically limit the survival of Leptospira, high livestock density and synanthropic reservoirs (e.g., rodents) may compensate, facilitating transmission. In this cross-sectional study, blood sera from 445 dairy cows (28 herds: 12 intensive [MI], 16 semi-intensive [MSI] systems) were analyzed via microscopic agglutination testing (MAT) against 10 pathogenic serovars. Urine samples were cultured for active Leptospira detection. Risk factors were assessed through epidemiological surveys and multivariable analysis. This study revealed an overall apparent seroprevalence of 27.0% (95% CI: 22.8–31.1), with significantly higher rates in MSI (54.1%) versus MI (12.2%) herds (p < 0.001) and an estimated true seroprevalence of 56.3% (95% CI: 50.2–62.1) in MSI and 13.1% (95% CI: 8.5–18.7) in MI herds (p < 0.001). The Sejroe serogroup was isolated from urine in both systems, confirming active circulation. In MI herds, rodent presence (OR: 3.6; 95% CI: 1.6–7.9) was identified as a risk factor for Leptospira seropositivity, while first-trimester abortions (OR:10.1; 95% CI: 4.2–24.2) were significantly associated with infection. In MSI herds, risk factors associated with Leptospira seropositivity included co-occurrence with hens (OR: 2.8; 95% CI: 1.5–5.3) and natural breeding (OR: 2.0; 95% CI: 1.1–3.9), whereas mastitis/agalactiae (OR: 2.8; 95% CI: 1.5–5.2) represented a clinical outcome associated with seropositivity. Despite semi-arid conditions, Leptospira maintains transmission in La Laguna, particularly in semi-intensive systems. The coexistence of adapted (Sejroe) and incidental serogroups underscores the need for targeted interventions, such as rodent control in MI systems and poultry management in MSI systems, to mitigate both zoonotic and economic impacts. Full article

The Mid-Piacenzian Warm Interval (MPWI) is marked by warmer temperatures and higher atmospheric CO₂ levels than today, making it an analogue for late-21st-century-warming, whereas the early Pleistocene cooling is more like today. We compare seasonal growth temperatures derived from oxygen isotope ratios (δ¹⁸O) and clumped isotopes (∆₄₇) in Mercenaria. Modern shells were previously collected from coastal NC. The fossil shells are from the Duplin (MPWI) and Waccamaw Formations (early Pleistocene), NC. Oxygen isotope ratios range from −2.2‰ to 2.3‰ (modern), −0.9‰ to 2.4‰ (MPWI), and −0.9‰ to 2.9‰ (early Pleistocene). The values of Δ₄₇ range from 0.576‰ to 0.639‰ (modern), 0.566‰ to 0.621‰ (MPWI), and 0.581‰ to 0.615‰ (early Pleistocene). We show that Mercenaria do not require a species-specific ∆₄₇ calibration. Modern and MPWI ∆₄₇-derived summer/winter temperatures (SST_∆47) and seasonal amplitudes are indistinguishable from δ¹⁸O-derived temperatures. The early Pleistocene summer SST_∆47 is indistinguishable from δ¹⁸O-derived temperatures, but the winter SST_∆47 is warmer by 5 °C and may reflect within-shell time averaging. The modern summer/winter SST_∆47 are indistinguishable from the MPWI, but the MPWI has a lower seasonal amplitude by 5 °C. Compared to our calculated δ¹⁸O_sw values, modeled values for the MPWI are within error but are much lower, and they are not within error for the early Pleistocene. Full article

Background/Objectives: To investigate acute caffeine (CAF: 375 mg, ≈4.8 mg/kg body mass) effects on energy expenditure (EE) and substrate kinetics during high-intensity interval exercise in individuals with high (HBAT) versus low (LBAT) brown adipose tissue activity using time-trend polynomial modeling. Methods: This is a randomized, double-blind crossover study in which 35 highly-trained males [HBAT-CAF, HBAT-PLA (Placebo), LBAT-CAF, LBAT-PLA] performed 30-min treadmill HIIE. Infrared thermography (IRT) assessed BAT activity by measuring supraclavicular skin temperature (SST). Breath-by-breath ergospirometry measured EE (kcal/min) and carbohydrate (CHO), lipid (LIP), and protein (PTN) oxidation. We applied second- and third-order polynomial regression models to depict the temporal trajectories of metabolic responses. Results: HBAT groups showed 25% higher sustained EE versus LBAT (p < 0.001), amplified by CAF. CHO oxidation exhibited biphasic kinetics: HBAT had 40% higher initial rates (0.75 ± 0.05 vs. 0.45 ± 0.04 g/min; p < 0.001) with accelerated decline (k = −0.21 vs. −0.15/min; p = 0.01). LIP oxidation peaked later in LBAT (40 vs. 20 min in HBAT), with CAF increasing oxidation by 18% in LBAT (p = 0.01). HBAT-CAF uniquely showed transient PTN catabolism (peak: 0.045 g/min at 10 min; k = −0.0033/min; p < 0.001). Conclusions: BAT status determines EE magnitude and substrate-specific kinetic patterns, while CAF exerts divergent modulation, potentiating early glycogenolysis in HBAT and lipolysis in LBAT. The HBAT-CAF synergy triggers acute proteolysis, revealing BAT-mediated metabolic switching. Full article

Postharvest storage of kiwifruit requires the implementation of precise environmental conditions to maintain fruit quality and reduce decay. In this research, conducted over two years, we examined whether the storage conditions, characterized by low temperature (1 ± 1 °C) and ultra-high relative humidity (higher than 99%, close to saturation), generated by the Xedavap^® machine from Xeda International, were effective in maintaining the fruit quality and reducing postharvest rots compared to standard storage conditions, characterized by involved low temperature (1 ± 1 °C) and high relative humidity (98%). Kiwifruits preserved under the experimental conditions exhibited a significantly lower rot incidence after 60 days of storage, with the treated fruits showing 4.48% rot compared to 23.03% under the standard conditions in the first year, using inoculated fruits, and 6.30% versus 9.20% in the second year using naturally infected fruits, respectively. After shelf life (second year only), rot incidence remained significantly lower in the treated fruits (12.80%) compared to the control (42.30%). Additionally, quality analyses showed better parameters when using the Xedavap^® system over standard methods. The ripening process was effectively slowed down, as indicated by changes in the total soluble solids, firmness, and titratable acidity compared to the control. These results highlight the potential of ultra-high relative humidity conditions to reduce postharvest rot, extend the shelf life, and enhance the marketability of kiwifruit, presenting a promising and innovative solution for the horticultural industry. Full article

Atmospheric warming results in increase in temperatures for the mean, the coldest, and the hottest day of the year, season, or month. Global warming leads to a large increase in the atmospheric water vapor content and to changes in the hydrological cycle, which include an intensification of precipitation extremes. Using the GISS-E2.1 climate model, we present the future changes in the coldest and hottest daily temperatures as well as in extreme precipitation indices (under four main Shared Socioeconomic Pathways (SSPs)). The increase in the wet-day precipitation ranges between 6% and 15% per 1 °C global surface temperature warming. Scaling of the 95th percentile versus the total precipitation showed that the sensitivity for the extreme precipitation to the warming is about 10 times stronger than that for the mean total precipitation. For six precipitation extreme indices (Total Precipitation, R95p, RX5day, R10mm, SDII, and CDD), the histograms of probability density functions become flatter, with reduced peaks and increased spread for the global mean compared to the historical period of 1850–2014. The mean values shift to the right end (toward larger precipitation and intensity). The higher the GHG emission of the SSP scenario, the more significant the increase in the index change. We found an intensification of precipitation over the globe but large uncertainties remained regionally and at different scales, especially for extremes. Over land, there is a strong increase in precipitation for the wettest day in all seasons over the mid and high latitudes of the Northern Hemisphere. There is an enlargement of the drying patterns in the subtropics including over large regions around Mediterranean, southern Africa, and western Eurasia. For the continental averages, the reduction in total precipitation was found for South America, Europe, Africa, and Australia, and there is an increase in total precipitation over North America, Asia, and the continental Russian Arctic. Over the continental Russian Arctic, there is an increase in all precipitation extremes and a consistent decrease in CDD for all SSP scenarios, with the maximum increase of more than 90% for R95p and R10 mm observed under SSP5–8.5. Full article

The development of an integrated circuit faces the challenge of the physical limit of Moore’s Law. One of the most important “Beyond Moore” challenges is the scaling down of Metal-Oxide-Semiconductor Field-Effect Transistors (MOSFETs) versus their increasing static power consumption. This is because, at room temperature, the thermal emission transportation mechanism will cause a physical limitation on subthreshold swing (SS), which is fundamentally limited to a minimum value of 60 mV/decade for MOSFETs, and accompanied by an increase in off-state leakage current with the process of scaling down. Moreover, the impacts of short-channel effects on device performance also become an increasingly severe problem with channel length scaling down. Due to the band-to-band tunneling mechanism, Tunnel Field-Effect Transistors (TFETs) can reach a far lower SS than MOSFETs. Recent research works indicated that TFETs are already becoming some of the promising candidates of conventional MOSFETs for ultra-low-power applications. This paper provides a review of some advances in materials and structures along the evolutionary process of TFETs. An in-depth discussion of both experimental works and simulation works is conducted. Furthermore, the performance of TFETs with different structures and materials is explored in detail as well, covering Si, Ge, III-V compounds and 2D materials, alongside different innovative device structures. Additionally, this work provides an outlook on the prospects of TFETs in future ultra-low-power electronics and biosensor applications. Full article

During high-current vacuum arcing, asymmetric arcing with off-center plasma columns may occur due to stochastic discharge initiation and mechanical motion, receiving less research attention than symmetric arcing. The objective of this paper is to numerically analyze the influence law of asymmetric arc ignition on arc parameters. For 60 mm diameter contacts, three arc conditions of symmetric arcing, 33% arc offset, and 67% arc offset were modeled. The results show that the arc offset causes asymmetry in the arc’s distribution. For 33% offset, the pressure and number density on the side away from the root of the arc is about 50% of root values, while these parameters fall below 20% for the 67% offset. Simultaneously, arc offset elevates peak parameter values: under 33% offset, maxima for ion pressure, ion density, ion temperature, electron temperature, and current density rise 12%, 11%, 6%, 6%, and 14% versus symmetric arcing; during 67% offset, these escalate significantly to 67%, 61%, 12%, 18%, and 47%. This study contributes to providing reference for the analysis of vacuum interruption processes under asymmetric arcing conditions. Full article

This study takes the daily temperature series of Taipei City as an example and proposes a data projection method based on Empirical Mode Decomposition (EMD), which effectively resolves the challenge of modeling non-stationary sequences. According to the daily mean temperature records from 1971 to 2023, Taipei has experienced an average warming rate of 0.02 °C per year. After applying EMD, the data were decomposed into 12 intrinsic mode functions (IMFs) and one residual trend. Among them, IMF5, with a period of 352 days (approximately one year), contributes 78% of the total energy, representing the dominant climatic cycle component. In this study, daily temperatures were categorized into five thermal levels: Cold (<12 °C), Cool (12–18 °C), Moderate (18–27 °C), Warm (27–32 °C), and Hot (>32 °C). In addition, using a 5-year moving process based on the annual EMD results, the IMFs and residuals were recombined to generate 390,625 synthetic sequences per year. Results show that the monthly mean temperatures of each year’s simulations closely match the observations, capturing the non-stationary characteristics of temperature variations. The overall classification accuracy of simulated versus observed daily temperature categories ranges from 60% to 71%, with an average of 65.1%. In summary, the EMD combined with the 5-year moving process developed in this study demonstrates a helpful data projection approach with effective reconstruction of periodic structures and stable simulation accuracy. It offers practical value for reconstructing urban climate variability, conducting risk assessments, and analyzing long-term warming trends. Moreover, it serves as a vital tool for modeling non-stationary climate data and supporting future projections. Full article

To address the high drying temperature, low yield, and low coating rate that characterize traditional chitosan/gum arabic microcapsules, this study used chitosan/sodium tripolyphosphate (STPP) ionic crosslinking to construct a composite wall, combined with optimized emulsifier compounding (T-80/SDBS), to prepare tung oil self-healing microcapsules. Orthogonal testing determined the following optimal parameters: a core-to-wall ratio of 2.0:1.0, a T-80/SDBS ratio of 4.0:6.0 (HLB = 12.383), an STPP concentration of 4%, and a spray-drying temperature of 120 °C. With these parameters, a yield of 42.91% and coating rate of 68.50% were achieved. The microcapsules were spherical (1–6 μm), with chitosan–STPP electrostatic interactions forming a dense wall. Adding 5% microcapsules to the UV topcoat enabled self-healing after 60 s UV curing: the scratch-healing rate reached 25.25% (width decreased from 11.13 μm to 8.32 μm), the elongation at break increased by 110% to 9.31%, the light transmission remained >82.50%, and the color difference (ΔE = 2.16) showed no significant change versus unmodified coating. Full article

Azole resistance in dermatophytes, particularly Trichophyton indotineae, has become a growing global concern. Current antifungal susceptibility testing protocols (EUCAST, CLSI) have limitations in reproducibility and sensitivity. This study aimed to evaluate how medium composition, incubation temperature, and spore concentration influence itraconazole susceptibility testing across various dermatophyte species. Thirty-eight clinical isolates representing Trichophyton, Microsporum, and Epidermophyton species were tested using a microplate laser nephelometry system (MLN). IC₅₀ values for itraconazole were determined in three different media (Sabouraud glucose (SG), RPMI-based (RG), and RG supplemented with casein (RGC)) at 28 °C and 34 °C. Effects of spore concentration on growth dynamics and lag phase were also analyzed. SG medium provided clear phenotypic separation between resistant and sensitive isolates. In contrast, RG and RGC showed overlapping IC₅₀ values. Lower spore concentrations revealed underlying growth differences, which were masked at higher inoculum levels. Temperature and media composition significantly affected IC₅₀ outcomes. Genotypic analysis confirmed resistance-associated Erg11B point mutations and genomic amplifications in T. indotineae, particularly in combination with Erg1 mutations, forming distinct subpopulations. SG medium combined with reduced spore concentrations offered improved differentiation of resistant versus sensitive strains. These findings support the development of more accurate susceptibility testing protocols and highlight the need to establish species-specific ECOFF values for dermatophytes. Full article

Global warming raises surface water temperatures, impacting fish alongside pollutants, such as ubiquitous xenoestrogens. Combined stressor effects are poorly studied but likely to worsen impacts and hinder biota adaptation, warranting further research. Unadapted fish face heightened risks. The liver is a vital metabolic organ, sensitive to temperature and xenoestrogens, eventually adjusting hepatocyte size and number to ensure survival, growth, and reproduction. This study assessed, for the first time, the impact of exposure (45 days) to thermal stress (29 °C versus 26 °C) and ethinylestradiol (EE2, 5 ng/L) on male guppies, primarily on body and quantitative liver morphology. Higher temperature reduced body mass (14%) and standard length (3.6%) gain. EE2 exposure reduced body mass increase (14%), hepatosomatic index (20%), and the volumes of the liver (32%), hepatocytes (16%), and their nuclei (17%). The nucleus-to-cytoplasm ratio and total hepatocyte number remained stable. No histopathological lesions existed. Guppies appear to have adapted to stressors by reducing hepatocyte size and utilizing lipid reserves, yet they exhibited deficits in body growth and hepatosomatic index. Gonadal maturation was unaffected. Only under EE2 at 29 °C did hepatocytes show minimal lipid droplet content (less vacuolation). This indicated exhausted reserves, reinforcing how heat and toxicants interact to exacerbate impacts. Full article

In airport operations management, accurately estimating the service durations of ground support equipment such as Potable Water Trucks (PWTs) is essential for improving resource allocation efficiency and ensuring timely aircraft turnaround. Traditional estimation methods often use fixed averages or assume normal distributions, failing to capture real-world variability and extreme scenarios effectively. To address these limitations, this study performs a comprehensive statistical analysis of PWT service durations using operational data from Beijing Daxing International Airport (ZBAD) and Shanghai Pudong International Airport (ZSPD). Employing chi-square goodness-of-fit tests, twenty probability distributions—including several heavy-tailed candidates—were rigorously evaluated under segmented scenarios, such as peak versus non-peak periods, varying temperature conditions, and different aircraft sizes. Results reveal that heavy-tailed distributions offer context-dependent advantages: the stable distribution exhibits superior modeling performance during peak operational periods, whereas the Burr distribution excels under non-peak conditions. Interestingly, contrary to existing operational assumptions, service durations at extremely high and low temperatures showed no significant statistical differences, prompting a reconsideration of temperature-dependent planning practices. Additionally, analysis by aircraft category showed that the Burr distribution best described service durations for large aircraft, while stable and log-logistic distributions were optimal for medium-sized aircraft. Numerical simulations confirmed these findings, demonstrating that the proposed heavy-tailed probabilistic models significantly improved resource prediction accuracy, reducing estimation errors by 13% to 25% compared to conventional methods. This research uniquely demonstrates the practical effectiveness of employing context-sensitive heavy-tailed distributions, substantially enhancing resource efficiency and operational reliability in airport ground handling management. Full article

Stream metabolism is traditionally defined as the combined metabolism of all aerobic organisms in a stream. Its component processes of oxygenic photosynthesis and aerobic respiration create and consume dissolved oxygen (DO) and therefore can be measured using time series of DO concentration, solar radiation, and water temperature, in conjunction with a model of DO dynamics that includes photosynthesis, respiration, and oxygen exchange with the atmosphere. A complication is that stream communities typically exhibit pronounced longitudinal heterogeneity in habitat type (e.g., shaded versus unshaded reaches) and species composition and abundance. The influence of a given stream reach and associated community on DO concentration propagates downstream with the current, gradually being replaced, over a transition zone, by the influence of the next downstream reach. Knowing the approximate length of this transition zone is important when estimating stream metabolism with one-station DO monitoring, since it indicates which stream reach and associated community the metabolism estimates apply to. We propose new methods for estimating the transition-zone length and for estimating the proportions of DO at a given location in a stream reach that entered the reach from upstream, from photosynthesis within the reach, and from atmospheric uptake within the reach. We also propose methods for estimating the residence-time distribution of DO present at a given stream location, and the corresponding distribution of upstream distances at which the DO entered the stream. Both distributions are approximately exponential. Thus, habitat immediately upstream of the sonde has the greatest influence on metabolism estimates with one-station monitoring, and it is therefore important to place the sonde so this habitat is representative of the study reach. Full article

In a warming climate, rising temperature are expected to influence atmospheric humidity. This study examined the spatio-temporal dynamics of temperature (TEMP) and relative humidity (RH) across Equatorial Africa from 1980 to 2020. The analysis used RH data from European Centre of Medium-range Weather Forecasts Reanalysis v.5 (ERA5) reanalysis, TEMP and precipitation (PRE) from Climate Research Unit (CRU), and soil moisture (SM) and evapotranspiration (ET) from the Global Land Evaporation Amsterdam Model (GLEAM). In addition, four teleconnection indices were considered: El Niño-Southern Oscillation (ENSO), Indian Ocean Dipole (IOD), North Atlantic Oscillation (NAO), and Pacific Decadal Oscillation (PDO). This study used the Mann–Kendall test and Sen’s slope estimator to analyze trends, alongside multiple linear regression to investigate the relationships between TEMP, RH, and key climatic variables—namely evapotranspiration (ET), soil moisture (SM), and precipitation (PRE)—as well as large-scale teleconnection indices (e.g., IOD, ENSO, PDO, and NAO) on annual and seasonal scales. The key findings are as follows: (1) mean annual TEMP exceeding 30 °C and RH less than 30% were concentrated in arid regions of the Sahelian–Sudano belt in West Africa (WAF), Central Africa (CAF) and North East Africa (NEAF). Semi-arid regions in the Sahelian–Guinean belt recorded moderate TEMP (25–30 °C) and RH (30–60%), while the Guinean coastal belt and Congo Basin experienced cooler, more humid conditions (TEMP < 20 °C, RH (60–90%). (2) Trend analysis using Mann–Kendal and Sen slope estimator analysis revealed spatial heterogeneity, with increasing TEMP and deceasing RH trends varying by region and season. (3) The warming rate was higher in arid and semi-arid areas, with seasonal rates exceeding annual averages (0.18 °C decade⁻¹). Winter (0.27 °C decade⁻¹) and spring (0.20 °C decade⁻¹) exhibited the strongest warming, followed by autumn (0.18 °C decade⁻¹) and summer (0.10 °C decade⁻¹). (4) RH trends showed stronger seasonal decline compared to annual changes, with reduction ranging from 5 to 10% per decade in certain seasons, and about 2% per decade annually. (5) Pearson correlation analysis demonstrated a strong negative relationship between TEMP and RH with a correlation coefficient of r = − 0.60. (6) Significant associations were also observed between TEMP/RH and both climatic variables (ET, SM, PRE) and large scale-teleconnection indices (ENSO, IOD, PDO, NAO), indicating that surface conditions may reflect a combination of local response and remote climate influences. However, further analysis is needed to distinguish the extent to which local variability is independently driven versus being a response to large-scale forcing. Overall, this research highlights the physical mechanism linking TEMP and RH trends and their climatic drivers, offering insights into how these changes may impact different ecological and socio-economic sectors. Full article

Background: Metronidazole is the preferred anaerobic agent for empiric treatment of intra-abdominal infections (IAI). Although dosed every 8 h (q8hr), blood concentrations exceed the in vitro minimum inhibitory concentration (MIC) for anaerobic organisms at 12 h (q12hr). A drug shortage of intravenous (IV) metronidazole prompted the conversion to every 12 h dosing in qualifying patients treated for IAI. Objective: To determine efficacy outcomes of metronidazole dosed every 12 h versus every 8 h in patients treated for IAI. Methods: This was a multi-center, retrospective, cohort study of 201 patients from January to July 2021 (q8hr) and January to November 2023 (q12hr) at five hospitals through the greater Houston area. Included patients were adults with a diagnosis of IAI confirmed by radiographic evidence and a white blood count (WBC) > 12,000 cells/µL and/or temperature > 100.4 °F at the time of diagnosis. The primary outcome was clinical cure of IAI, defined as resolution of signs/symptoms of IAI and normalization of WBC or temperature. Results: A total of 201 patients were included, 103 patients in the q8hr group and 98 patients in the q12hr group. Clinical cure of IAI occurred in 72 patients (69.9%) in the q8hr group and 62 patients (63.2%) in the q12hr group (p = 0.318). The median duration of therapy days was similar for both groups (4.0 [4.0–6.0] vs. 4.0 [3.0–6.0] (p = 0.509)). The frequency of clinical failure was higher in the q12hr group (8.7% vs. 21.4%; p = 0.01). Seven patients in the q8hr group and fourteen patients in the q12hr group required escalation of antibiotics due to the need for broader-spectrum antimicrobial therapy by clinical failure definition. Conclusions: There was no difference in clinical cure of IAI with an extended dosing interval. Clinical failure and escalation in antibiotics was higher in the q12h group due to the need for broader-spectrum gram-negative coverage and not related to the need for anaerobic coverage. Findings suggest that every 12 h dosing has similar outcomes. Full article