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Article

3 Citations

3,913 Views

18 Pages

Primary-like Human Hepatocytes Genetically Engineered to Obtain Proliferation Competence as a Capable Application for Energy Metabolism Experiments in In Vitro Oncologic Liver Models

Andrea Scheffschick,
Jonas Babel,
Sebastian Sperling,
Julia Nerusch,
Natalie Herzog,
Daniel Seehofer and
Georg Damm

Biology2022, 11(8), 1195;https://doi.org/10.3390/biology11081195

9 August 2022

Non-alcoholic fatty liver disease (NAFLD), characterized by lipid accumulation in the liver, is the most common cause of liver diseases in Western countries. NAFLD is a major risk factor for developing hepatocellular carcinoma (HCC); however, in vitr...

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Review

43 Citations

6,246 Views

19 Pages

Three-Dimensional Liver Culture Systems to Maintain Primary Hepatic Properties for Toxicological Analysis In Vitro

Sarah Kammerer

Int. J. Mol. Sci.2021, 22(19), 10214;https://doi.org/10.3390/ijms221910214

23 September 2021

Drug-induced liver injury (DILI) is the major reason for failures in drug development and withdrawal of approved drugs from the market. Two-dimensional cultures of hepatocytes often fail to reliably predict DILI: hepatoma cell lines such as HepG2 do...

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Article

1 Citations

1,424 Views

26 Pages

Investigation and Distinction of Energy Metabolism in Proliferating Hepatocytes and Hepatocellular Carcinoma Cells

Julia Nerusch,
Gerda Schicht,
Natalie Herzog,
Jan-Heiner Küpper,
Daniel Seehofer and
Georg Damm

Cells2025, 14(16), 1254;https://doi.org/10.3390/cells14161254

14 August 2025

Metabolic rewiring is a hallmark of both hepatic regeneration and malignant transformation, complicating the identification of cancer-specific traits. This study aimed to distinguish the metabolic profiles of proliferating hepatocytes and hepatocellu...

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Article

3 Citations

3,053 Views

29 Pages

Human Multi-Lineage Liver Organoid Model Reveals Impairment of CYP3A4 Expression upon Repeated Exposure to Graphene Oxide

Alessio Romaldini,
Raffaele Spanò,
Marina Veronesi,
Benedetto Grimaldi,
Tiziano Bandiera and
Stefania Sabella

Cells2024, 13(18), 1542;https://doi.org/10.3390/cells13181542

13 September 2024

Three-dimensional hepatic cell cultures can provide an important advancement in the toxicity assessment of nanomaterials with respect to 2D models. Here, we describe liver organoids (LOs) obtained by assembling multiple cell lineages in a fixed ratio...

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Article

12 Citations

3,590 Views

15 Pages

Valproate and Short-Chain Fatty Acids Activate Transcription of the Human Vitamin D Receptor Gene through a Proximal GC-Rich DNA Region Containing Two Putative Sp1 Binding Sites

Marta Moreno-Torres,
Carla Guzmán,
Petar D. Petrov and
Ramiro Jover

Nutrients2022, 14(13), 2673;https://doi.org/10.3390/nu14132673

28 June 2022

The vitamin D receptor (VDR) mediates 1,25-dihydroxyvitamin D3 pleiotropic biological actions through transcription regulation of target genes. The expression levels of this ligand-activated nuclear receptor are regulated by multiple mechanisms both...

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