Search Results (2,479)

Due to their nutritional value and sustainability, edible insect-based foods are gaining popularity in Europe. Their use is regulated by EU legislation, which defines authorised species and sets labelling requirements. Molecular tools are being developed to authenticate such products. In this study, yellow mealworm (Tenebrio molitor) larvae authorised for human consumption were fed wheat flour-based diets containing varying proportions of house cricket (Acheta domesticus) flour for 21 days. This was followed by a 48 h starvation period to assess the persistence of insect DNA in the digestive tract. Two novel, species-specific, single-copy markers were designed: ampd gene for the Acheta domesticus and MyD88 gene for the Tenebrio molitor. These were applied using qPCR and ddPCR. Both methods successfully detected cricket DNA in the guts of starved larvae. Linear regression analysis revealed a strong, statistically significant correlation between the proportion of Acheta domesticus flour in the diet and the normalised relative quantity of DNA. ddPCR proved to be more sensitive than qPCR, particularly in the detection of low DNA levels. These results suggest that the presence of DNA from undeclared insect species in edible insects may be indicative of their diet rather than contamination or adulteration. This highlights the importance of contextual interpretation in food authenticity testing. Full article

Objectives: Pelvic tumor resections demand high surgical precision to ensure clear margins while preserving function. Although patient-specific instruments (PSIs) improve osteotomy accuracy, positioning errors remain a limitation. This study evaluates the feasibility, accuracy, and usability of a novel dual-function augmented reality (AR) system for intraoperative guidance in PSI positioning and osteotomy execution using a head-mounted display (HMD). The system provides dual-function support by assisting both PSI placement and osteotomy execution. Methods: Ten fresh-frozen cadaveric hemipelves underwent AR-assisted internal hemipelvectomy, using customized 3D-printed PSIs and a new in-house AR software integrated into an HMD. Angular and translational deviations between planned and executed osteotomies were measured using postoperative CT analysis. Absolute angular errors were computed from plane normals; translational deviation was assessed as maximum error at the osteotomy corner point in both sagittal (pitch) and coronal (roll) planes. A Wilcoxon signed-rank test and Bland–Altman plots were used to assess intra-workflow cumulative error. Results: The mean absolute angular deviation was 5.11 ± 1.43°, with 86.66% of osteotomies within acceptable thresholds. Maximum pitch and roll deviations were 4.53 ± 1.32 mm and 2.79 ± 0.72 mm, respectively, with 93.33% and 100% of osteotomies meeting translational accuracy criteria. Wilcoxon analysis showed significantly lower angular error when comparing final executed planes to intermediate AR-displayed planes (p < 0.05), supporting improved PSI positioning accuracy with AR guidance. Surgeons rated the system highly (mean satisfaction ≥ 4.0) for usability and clinical utility. Conclusions: This cadaveric study confirms the feasibility and precision of an HMD-based AR system for PSI-guided pelvic osteotomies. The system demonstrated strong accuracy and high surgeon acceptance, highlighting its potential for clinical adoption in complex oncologic procedures. Full article

The increasing demand for sustainable construction materials has driven research into low-carbon geopolymers that mitigate both cement-related emissions and plastic and glass waste accumulation. This study explores the development of geopolymer concrete incorporating fly ash (FA), slag (S), and FA + S blends, with 10% recycled crushed glass (RCG) and recycled plastic waste (RPW) as partial coarse aggregate replacements. Compressive strength testing revealed that FA + S-based geopolymers (25FA + S) with 100% ordinary Portland cement (OPC) replacement achieved a 7-day strength of 24.6 MPa, representing a 98% improvement over control specimens. Slag-based geopolymers demonstrated water absorption properties comparable to OPC, indicating enhanced durability. Microstructural analyses using SEM, XRD, and EDS confirmed the formation of a dense aluminosilicate matrix, with slag promoting FA reactivity and reinforcing interfacial transition zone (ITZ). These effects contributed to superior mechanical performance and water resistance. Despite minor shrinkage-induced cracking, full OPC replacement with S or FA + S geopolymers outperformed control specimens, consistently exceeding the target strength of 15 MPa required for low-impact, single-story housing applications within seven days. These findings underscore the potential of geopolymer systems for rapid and sustainable construction, offering an effective solution for reducing carbon footprints and repurposing industrial waste. Full article

The electromechanical braking (EMB) system is an important component of intelligent vehicles and is also the core actuator for longitudinal dynamic control in autonomous driving motion control. Therefore, we propose a new mechanism layout form for EMB and a feedforward second-order linear active disturbance rejection controller based on clamping force. This solves the problem of excessive axial distance in traditional EMB and reduces the axial distance by 30%, while concentrating the PCB control board for the wheels on the EMB housing. This enables the ABS and ESP functions to be integrated into the EMB system, further enhancing the integration of line control and active safety functions. A feedforward second-order linear active disturbance rejection controller (LADRC) based on the clamping force of the brake caliper is proposed. Compared with the traditional clamping force control methods three-loop PID and adaptive fuzzy PID, it improves the response speed, steady-state error, and anti-interference ability. Moreover, the LADRC has more advantages in parameter adjustment. Simulation results show that the response speed is increased by 130 ms, the overshoot is reduced by 9.85%, and the anti-interference ability is increased by 41.2%. Finally, the feasibility of this control algorithm was verified through the EMB hardware-in-the-loop test bench. Full article

The research was initiated by the Plečnik House gallery (Ljubljana, Slovenia) and focuses on the 3D architectural reconstruction of the unrealised monument of the Czech military leader Jan Žižka, designed by the Slovenian architect Jože Plečnik. In addition, the experience with the 3D reconstructed monument in the exhibition “Plečnik and the Sacred” was analysed. Using the available references and interpretative approaches, a digital and 3D-printed reconstruction was created that retains Plečnik’s architectural style. The experimental phase included a detailed interpretation of the studied references, 3D modelling, 3D printing, exhibition and experience analysis. The dimensions of the finished 3D-printed model are 52.80 × 55.21 × 44.60 cm. It was produced using stereolithography (SLA) for figurative elements and fused deposition modelling (FDM) for architectural components. The reconstruction was evaluated using participant testing, including semantic differential analysis, comparative studies, and knowledge-based questionnaires. The results showed that architectural elements were reconstructed with an average similarity score of 1.97 out of 5. Statues followed with a score of 1.81, and props, though detailed, met audience expectations, scoring 1.61. Clothing received the lowest score of 1.40. This research emphasises the importance of a hypothetical digital 3D reconstruction of never constructed monument for broader understanding of Plečnik’s legacy. Full article

People with severe mental disorders (SMD) face long-term functional impairments requiring integrated, community-based, recovery-oriented care. Italy provides two main housing models for people with SMD: private accommodation (PA) and supported accommodation (SA). This exploratory study investigated differences in recovery outcomes across these settings using the Mental Health Recovery Star (MHRS). A six-month longitudinal study was conducted within the South Verona Community Mental Health Service. Nineteen trained mental health professionals assessed 25 people with SMD (14 in PA, 11 in SA) at baseline (BL) and follow-up (FU) using standardized tools for recovery (MHRS), functioning, psychopathology, functional autonomy, and needs. Group comparisons and within-group changes were analyzed using paired and independent t-tests. At BL, people with SMD in PA showed better functioning (p = 0.040) and fewer needs than those in SA (p = 0.008). Recovery goals differed, with people with SMD in PA focusing on health and networks, while people with SMD in SA emphasized functioning. At FU, people with SMD in PA improved across all MHRS domains (p < 0.001), with significant reductions in symptom severity and unmet needs. People with SMD in SA showed targeted improvements in functioning, autonomy, and MHRS social networks (p < 0.001), with increases in met needs but non-significant changes in unmet needs. When comparing PA and SA at FU, the differences were relatively modest. Recovery is achievable in both housing settings, although outcomes differ. People with SMD in PA experienced broader improvements, while people with SMD in SA progressed in their prioritized areas, likely reflecting more complex initial needs. These findings underscore the value of aligning recovery-oriented care with the specific needs and contexts of different residential settings. Further research is needed to confirm and expand these results. Full article

Background/Objectives: The Rapid Access and Stabilization Program (RASP), launched in Nova Scotia in April 2023, aims to improve timely psychiatric care, reduce reliance on emergency services, and provide early intervention. This study describes the sociodemographic and clinical characteristics of the RASP participants and examines their association with acute service use. Methods: This cross-sectional descriptive study used self-reported surveys and administrative data from 738 RASP participants. Descriptive statistics summarized key sociodemographic and clinical variables. Associations between these characteristics and acute service use (emergency department visits, inpatient admissions, and mobile crisis calls) were examined using chi-square and Fisher’s Exact tests. Bonferroni correction was applied for multiple comparisons. Results: The sample was predominantly female (65.2%) and aged 20–40 years (38.4%). Despite high rates of severe anxiety (53.9%) and depression (36.0%), acute service use was low: emergency department visits (7.2%), mobile crisis calls (1.0%), and inpatient admissions (0.8%). Preliminary analyses showed that education level and housing status were associated with ED visits and inpatient admissions. However, these associations did not remain statistically significant after Bonferroni correction. Conclusions: Although mental health symptom severity was high, acute mental health service use remained low after RASP enrollment, indicating the program’s potential in reducing reliance on crisis services. No participant characteristics were significantly associated with acute service use after adjustment, underscoring the complexity of predicting utilization and the need for robust multivariable models. Continued investment in rapid access programs may be essential to improving timely mental health care and supporting early intervention strategies. Full article

Triple-negative breast cancer (TNBC) remains a major clinical challenge due to its aggressive behavior and lack of targeted therapies. Accurate early prediction of response to neoadjuvant chemotherapy (NACT) is essential for guiding personalized treatment strategies and improving patient outcomes. In this study, we present an attention-based multiple instance learning (MIL) framework designed to predict pathologic complete response (pCR) directly from pre-treatment hematoxylin and eosin (H&E)-stained biopsy slides. The model was trained on a retrospective in-house cohort of 174 TNBC patients and externally validated on an independent cohort (n = 30). It achieved a mean area under the curve (AUC) of 0.85 during five-fold cross-validation and 0.78 on external testing, demonstrating robust predictive performance and generalizability. To enhance model interpretability, attention maps were spatially co-registered with multiplex immunohistochemistry (mIHC) data stained for PD-L1, CD8+ T cells, and CD163+ macrophages. The attention regions exhibited moderate spatial overlap with immune-enriched areas, with mean Intersection over Union (IoU) scores of 0.47 for PD-L1, 0.45 for CD8+ T cells, and 0.46 for CD163+ macrophages. The presence of these biomarkers in high-attention regions supports their biological relevance to NACT response in TNBC. This not only improves model interpretability but may also inform future efforts to identify clinically actionable histological biomarkers directly from H&E-stained biopsy slides, further supporting the utility of this approach for accurate NACT response prediction and advancing precision oncology in TNBC. Full article

This study provides a detailed dataset from two modern homes constructed inside an environmentally controlled chamber. These data are used to carefully calibrate a dynamic thermal simulation model of these homes. The calibrated models show good agreement with measurements taken under controlled conditions. The two case study homes, “The Future Home” and “eHome2”, were constructed within the University of Salford’s Energy House 2.0, and high-quality data were collected over eight days. The calibration process involved updating U-values, air permeability rates, and modelling refinements, such as roof ventilation, ground temperatures, and sub-floor void exchange rates, set as boundary conditions. Results demonstrated a high level of accuracy, with performance gaps in whole-house heat transfer coefficient reduced to 0.5% for “The Future Home” and 0.6% for “eHome2”, falling within aggregate heat loss test uncertainty ranges by a significant amount. The study highlights the improved accuracy of calibrated dynamic thermal simulation models, compared to results from the steady-state Standard Assessment Procedure model. By providing openly accessible calibrated models and a clearly defined methodology, this research presents valuable resources for future building performance modelling studies. The findings support the UK’s transition to dynamic modelling approaches proposed in the recently introduced Home Energy Model approach, contributing to improved prediction of energy efficiency and aligning with goals for zero carbon ready and sustainable housing development. Full article

As high-speed electric multiple units (EMUs) advance in speed and complexity, quasi-static design methods may underestimate the fatigue risks associated with high-frequency dynamic excitations. This study quantifies the contribution of gear meshing-induced vibrations (2512 Hz) to fatigue damage in EMU gearbox housings, revealing resonance amplification of local stresses up to 1.8 MPa at 300 km/h operation. Through integrated field monitoring and bench testing, we demonstrated that gear meshing excites structural modes, generating sustained, very high-cycle stresses (>10⁸ cycles). Crucially, fatigue specimens were directly extracted from in-service gearbox housings—overcoming the limitations of standardized coupons—passing the very high-cycle fatigue (VHCF) test to derive S-N characteristics beyond 10⁸ cycles. Results show a continuous decline in fatigue strength (with no traditional fatigue limit) from 10⁸ to 10⁹ cycles. This work bridges the gap between static design standards (e.g., FKM) and actual dynamic environments, proving that accumulated damage from low-amplitude gear-meshing stresses (3.62 × 10¹¹ cycles over a 12 million km lifespan) contributes to a 16% material utilization ratio. The findings emphasize that even low-magnitude gear-meshing stresses can significantly influence gearbox fatigue life due to their ultra-high frequency, warranting design consideration beyond current standards. Full article

The Centers for Diseases Control (CDC) light trap is widely used for malaria vector surveillance, but its acquisition logistics pose challenges in Africa. Evaluating new traps can improve surveillance tools. This study compared the efficiency of the BG-Pro UV and Silver Bullet 2.1 UV (SB 2.1 UV) against the UV LED CDC trap in western Kenya’s rice irrigation area. The traps were tested indoors in eight houses over 64 nights. Light properties and fan speed were analyzed using spectrometry and an anemometer. The BG-Pro UV trap performed better than the UV LED CDC trap for An. gambiae s.l. (RR 2.0, 95% CI 0.9–3.9) and An. funestus s.l. (RR 3.5, 95% CI 1.9–6.4). The SB 2.1 UV trap was more effective in capturing An. gambiae s.l. (RR 4.3, 95% CI 2.5–7.3) and An. funestus s.l. (RR 7.1, 95% CI 3.9–13.1), and also caught three times more Culex spp. (RR 2.7, 95% CI 1.2–6.0). SB 2.1 UV had the highest downstream force, and all traps emitting UV-A light had consistent wavelengths. Overall, the BG-Pro and SB 2.1 traps’ trapping efficiency was three to six times more than the CDC trap, making them promising surveillance tools, particularly in low-density malaria settings. Full article

Odors from pet cats can negatively affect the quality of life of cat owners. The diverse bioactive compounds in plant extracts make them a promising candidate for effective odor reduction. This study evaluated twelve plant extracts for deodorizing efficacy via in vitro fermentation tests. Rosemary extract and licorice extract exhibited better deodorizing effects, with fractions of rosemary extract below 100 Da demonstrating the most effective deodorizing performance. Based on these findings, subsequent feeding trials were conducted using rosemary extract and its fractions below 100 Da. In the feeding trial, adult British Shorthair cats were divided into three groups (Control Check, RE, and RE100) and housed in a controlled-environment respiration chamber for 30 days. Measurements included odor emissions, fecal and blood physicochemical parameters, immune parameters, microbiota composition based on 16S rRNA sequencing, and metabolome analysis. The results of the feeding trial indicated that rosemary extract significantly reduced ammonia and hydrogen sulfide emissions (46.84%, 41.64%), while fractions below 100 Da of rosemary extract achieved even greater reductions (55.62%, 53.87%). Rosemary extract regulated the intestinal microbial community, significantly increasing the relative abundance of the intestinal probiotic Bifidobacterium (p < 0.05) and reducing the population of sulfate-reducing bacteria (p < 0.05). It also significantly reduced urease and uricase activities (p < 0.05) to reduce ammonia production and inhibited the degradation of sulfur-containing proteins and sulfate reduction to reduce hydrogen sulfide emissions. Furthermore, rosemary extract significantly enhanced the immune function of British Shorthair cats (p < 0.05). This study suggests that rosemary extract, particularly its fractions below 100 Da, is a highly promising pet deodorizer. Full article

Equine influenza is a highly contagious disease caused by the equine influenza virus (EIV). The occurrence of EIV outbreaks in America is associated with low levels of vaccination coverage. In Colombia, no seroprevalence evaluation has been carried out to estimate the distribution of the virus within the country. Our aim was to perform a sero-epidemiological survey of equine influenza infections and to identify associated risk factors in horses from four departments of Colombia. Serological testing was carried out by using an ELISA for the detection of IgG antibodies against the influenza A virus. The evaluation of epidemiological variables, clinical manifestations, and vaccination history was carried out through the application of a data collection instrument. Among the 385 horses analyzed, 27% of the samples tested positive, with a higher prevalence in Study 1 from horses with respiratory symptoms (40.4%) than in Study 2 from horses without clinical signs (16.1%). Only horses housed in stables had higher odds of testing positive. The study also revealed that unvaccinated horses were 68% less likely to test positive than vaccinated horses were. This research highlights a significant gap in vaccination coverage and the presence of antibodies even in asymptomatic horses. Management factors such as activity type and housing should be considered when strategies for EIV prevention are developed. Full article

We present a miniaturized, inexpensive, and user-friendly microfluidic platform to support biological applications. The system integrates a mini-incubator providing controlled environmental conditions and housing a microfluidic device for long-term cell culture experiments. The incubator is designed to be compatible with standard inverted optical microscopes and Raman spectrometers, allowing for the non-invasive imaging and spectroscopic analysis of cell cultures in vitro. The microfluidic device, which reproduces a dynamic environment, was optimized to sustain a passive, gravity-driven flow of medium, eliminating the need for an external pumping system and reducing mechanical stress on the cells. The platform was tested using Raman analysis and adherent tumoral cells to assess proliferation prior and subsequent to hydrogen peroxide treatment for oxidative stress induction. The results demonstrated a successful adhesion of cells onto the substrate and their proliferation. Furthermore, the platform is suitable for carrying out optical monitoring of cultures and Raman analysis. In fact, it was possible to discriminate spectra deriving from control and hydrogen peroxide-treated cells in terms of DNA backbone and cellular membrane modification effects provoked by reactive oxygen species (ROS) activity. The 800–1100 cm⁻¹ band highlights the destructive effects of ROS on the DNA backbone’s structure, as its rupture modifies its vibration; moreover, unpaired nucleotides are increased in treated sample, as shown in the 1154–1185 cm⁻¹ band. Protein synthesis deterioration, led by DNA structure damage, is highlighted in the 1257–1341 cm⁻¹, 1440–1450 cm⁻¹, and 1640–1670 cm⁻¹ bands. Furthermore, membrane damage is emphasized in changes in the 1270, 1301, and 1738 cm⁻¹ frequencies, as phospholipid synthesis is accelerated in an attempt to compensate for the membrane damage brought about by the ROS attack. This study highlights the potential use of this platform as an alternative to conventional culturing and analysis procedures, considering that cell culturing, optical imaging, and Raman spectroscopy can be performed simultaneously on living cells with minimal cellular stress and without the need for labeling or fixation. Full article

Mycoplasma pneumoniae is a significant causative agent of atypical pneumonia in both children and adults. Timely and accurate diagnosis is crucial for appropriate patient management. Conventional methods for detecting M. pneumoniae, such as culture and serology, exhibit several limitations regarding sensitivity, specificity, and turnaround time. In contrast, real-time PCR is considered the most reliable, rapid, and sensitive technique for the diagnosis of M. pneumoniae infection. In this study, we adapted and validated an in-house real-time PCR assay for use on the fully automated Panther Fusion^® System. The validation process included two artificial samples, five external quality controls, and sixty-two patient samples. We evaluated the performance in terms of precision, sensitivity, linearity, and analytical sensitivity, comparing it to the original in-house assay. The Panther Fusion^® System demonstrated a broad dynamic range (16–1.6 × 10⁷ copies/reaction), a robust correlation (94%) with the in-house assay, and comparable sensitivity (46 copies/mL vs. 25 copies/mL). The concordance between the in-house real-time PCR and the Panther Fusion^® System was 100% for both clinical samples and external quality controls. The adaptation of the test to the Panther Fusion^® System enabled the inclusion of M. pneumoniae among the pathogens monitored for respiratory infection surveillance. Throughout 2024, we analyzed 2567 samples, with a peak positivity rate of 38% observed in August. These findings underscore the significance of employing the M. pneumoniae diagnostic assay on the Panther Fusion^® System which proves valuable for the detection of M. pneumoniae infections. This platform offers the advantages of increased automation and greater throughput potential compared to other platforms, enhancing the efficiency of respiratory pathogen detection in clinical settings. Full article