Search Results (346)

The growing demand for clean-label gluten-free bread is driving a reduction in additives, although their technological roles are not yet fully understood. This study evaluated the effect of progressively removing monocalcium phosphate, sodium bicarbonate, and mono- and diglycerides (MDG) on the quality of gluten-free bread during storage. Four formulations were prepared: a reference (RF) containing all additives, and three reduced-additive versions without monocalcium phosphate (FA), without monocalcium phosphate and sodium bicarbonate (FB), or without any additives (FC). Specific volume, moisture, water activity, crumb structure, color, and texture were assessed on days 1, 8, 15, and 22. Additive removal significantly affected bread quality: the formulation without leavening agents (FB) showed the lowest specific volume (≈2.8 cm³/g) and the highest crumb hardness (≈38 N), whereas the additive-free formulation (FC) achieved the highest specific volume (≈3.3 cm³/g) and a crumb structure comparable to the reference bread, with a higher void fraction (≈28%). During storage, all breads exhibited increasing hardness, although FC did not stale faster than RF, likely due to its higher specific volume after baking. The results confirm that sodium bicarbonate and monocalcium phosphate are essential for gas generation and structural development, while removal of MDG improved loaf volume without intensifying deterioration. Full article

Background/Objectives: Poor dietary habits are a major contributor to non-communicable diseases (NCDs), the leading cause of mortality worldwide. To promote healthier eating, governments and stakeholders have implemented various nutrition policies, including front-of-pack nutrition labeling (FOPNL). The Choices International Foundation (Choices), through its criteria, supports these efforts through its standardized nutrient profiling system (NPS). Originally developed to underpin a positive FOPNL logo, in 2021, the criteria were expanded into a globally oriented five-level profiling system covering 23 basic and 10 discretionary food groups, addressing key nutrients such as trans-fatty acids, saturated fat, sodium, sugar, fiber, and energy. To ensure continued scientific relevance, the Choices criteria are periodically reviewed by an independent International Scientific Committee (ISC). Methods: This paper presents the 2025 revision of the Choices criteria, focusing on priority areas identified through stakeholder consultation and recent scientific developments. Results: Key updates include the introduction of nutrient-based equivalence criteria for plant-based alternatives to meat and dairy, based on protein and selected micronutrient thresholds. Non-sugar sweeteners (NSSs) were newly included as a factor that lowers a product’s health classification and makes it ineligible for a positive FOPNL. Additionally, the industrially produced trans-fatty acid (iTFA) criteria were revised and aligned with the latest World Health Organization (WHO) recommendations, improving both technical feasibility and policy coherence. While options for incorporating whole-grain and micronutrient criteria were explored, these were not included in the current revision. Conclusions: The 2025 update system enhances the scientific rigor, policy alignment, and global applicability of the Choices system. By providing a harmonized and evidence-based tool, it aims to support national policies that foster healthier food environments and, ultimately, improve public health outcomes worldwide. Full article

Sodium hypochlorite (NaClO) is widely used to purify oocysts in laboratories. While previous studies have extensively examined its effects on oocyst viability, pathogenicity, and sporulation rate, the impact of NaClO treatment on proteomic profiles remains uncharacterized. Transmission electron microscopy was used in the present study to characterize structural changes in unsporulated oocyst walls of Eimeria tenella treated with NaClO. The results indicated that NaClO treatment destroyed the bilayer wall of unsporulated oocysts, stripping away the outer wall and making the inner layer thicker. Label-free quantitative proteomics was employed to identify differentially expressed proteins (DEPs) in NaClO-treated (Et-T) and untreated (Et-C) unsporulated oocysts. Among 2422 identified proteins, 1345 were differentially expressed, with 1210 upregulated and 134 downregulated in Et-T vs. Et-C. Functional analysis revealed that upregulated proteins are predominantly associated with oocyst wall biosynthesis and cellular stress responses, whereas downregulated proteins are involved in outer wall assembly and structural integrity. Notably, 12 proteins—including 9 hypothetical proteins, acid phosphatase, adenylate cyclase, and microneme protein 2—were exclusively detected in the Et-C, indicating their potential essentiality in outer wall formation. These findings reveal the structure and protein composition of the oocyst wall of E. tenella, supporting research on its biosynthesis and environmental resilience. Full article

Background: Inflammatory bowel diseases (IBD) rely on invasive methods for detecting intestinal inflammation, with the needs for non-invasive molecular imaging tools being unmet. Integrin α4β7 is a key target in IBD pathogenesis due to its role in the recruitment of T cells. This study aimed to develop a novel ⁶⁸Ga-labeled integrin α4β7-targeted radiopharmaceutical (⁶⁸Ga-A2) and evaluate its feasibility for non-invasive PET/CT imaging of IBD inflammation in a dextran sulfate sodium (DSS)-induced murine colitis model. Methods: ⁶⁸Ga-A2 was synthesized via radiolabeling DOTA-A2 with ⁶⁸Ga. In vitro properties (radiochemical purity, stability, binding specificity, and affinity) of ⁶⁸Ga-A2 were validated. The DSS-induced colitis model was established and confirmed in C57BL/6J mice, followed by in vivo PET/CT imaging, ex vivo biodistribution studies, and histological (HE and IHC) analyses to evaluate the targeting efficacy of ⁶⁸Ga-A2. Results: ⁶⁸Ga-A2 was prepared efficiently (20 min) with a radiochemical purity of >95% and demonstrated good in vitro stability. It exhibited specific binding to integrin α4β7 with a Kd of 68.48 ± 6.55 nM. While whole-body PET/CT showed no visible inflammatory focus uptake, ex vivo imaging and biodistribution of colon tissue revealed significantly higher uptake in DSS-treated mice compared to that in healthy/blocking groups, which was consistent with histological evidence of inflammation. Conclusions: ⁶⁸Ga-A2 demonstrated specific targeting of IBD inflammatory foci in vitro and ex vivo. Despite whole-body imaging limitations, further optimization of its structure may enable it to become a promising non-invasive PET agent for IBD. These findings support future clinical investigations to validate its utility in IBD diagnosis and monitoring. Full article

In this paper, the conceptual hydrogeological circulation model of natural mineral waters from Ribeirinho and Fazenda do Arco hydromineral concession (Castelo de Vide) is updated. These waters are exploited by the Super Bock Group, as bottled waters, and are commercially labeled as Água Vitalis. The physico-chemical data (2004–2024) of these waters were processed regarding their joint interpretation with recent isotopic (δ²H and δ¹⁸O) data. The study region is dominated by the Castelo de Vide syncline, which develops along the southern limit of the Central Iberian Zone. These natural mineral waters have low electrical conductivity (EC) mean values (42.80 < EC_mean < 54.45 μS/cm) and a slightly acidic pH (5.14 < pH_mean < 5.46), making them hyposaline waters. The recharge area of this aquifer system coincides fundamentally with the outcrops of Lower Ordovician quartzites. The updated conceptual circulation model presented in this work is essentially developed on the basis of the chloride–sodium signatures of these waters, explained by the preferential recharge of meteoric waters (δ²H and δ¹⁸O) and low water–rock interaction temperature. Such isotopic results seem to indicate the non-existence of a flow continuity between the two blocks (NW and SE) of the quartzite ridges, separated by a fault with a local orientation approximately N-S, as indicated by the most enriched isotopic values of the waters from borehole AC22 (δ¹⁸O = −5.90‰ vs. V-SMOW) located in the SE block, compared to the average isotopic value of the waters from the other boreholes (Vitalis I, II, III, IV, V and VI) located in the NW block (δ¹⁸O_mean = −6.30‰ vs. V-SMOW). This study enhances the understanding of the hydrogeological and geochemical processes controlling low-mineralized (hyposaline) natural mineral waters, widely used for therapeutic and commercial purposes. Despite their global importance, detailed hydrogeological and isotopic studies of such systems are still scarce, making this conceptual model a valuable reference for their sustainable management. Full article

The aim of this study was to evaluate the nutritional composition of beef from five autochthonous calving breeds from Southwest Spain (Retinta, Pajuna, Marismeña, Berrenda en Colorado, and Lidia) reared under their traditional production systems. Longissimus dorsi samples were analyzed for pH, fat, moisture, ash protein content, mineral composition, fatty acid profile, and volatile compounds. Carcass weights of calves ranged from 108 to 328 kg according to the Spanish market, with significant breed differences in fat (range 2.98–8.41%), moisture (69.47–72.62%), and protein (20.98–23.82%), but not in ash (1.03–1.17%). Sodium levels were below 120 mg/100 g, allowing all breeds to be classified as low-sodium, while phosphorus values supported a high-phosphorus label. The Pajuna, Berrenda en Colorado, and Lidia breeds showed higher levels of beneficial fatty acids such as EPA, DPA, DHA, and CLA, with n-6/n-3 ratios ≤ 4, while Retinta and Marismeña presented higher ratios (6.09 and 5.23, respectively). The breeds from Southwest Spain stand out for their content in ketone, ester, and aromatic hydrocarbon volatile compounds linked to the intake of grass, forage, and food concentrate. These results highlight the favorable nutrient profiles and distinctive traits of Spanish autochthonous cattle breeds, emphasizing their value in sustainable production and conservation programs. Full article

Authenticating the geographical origin of honey is crucial for ensuring its quality and preventing fraudulent labeling. This study investigates the influence of altitude on the mineral composition of honey and comparatively evaluates the performance of chemometric and machine learning models for its geographic discrimination. Honey samples from three distinct altitude regions in Türkiye were analyzed for their mineral content using Inductively Coupled Plasma-Mass Spectrometry (ICP-MS). Results revealed that Calcium (Ca), Potassium (K), and Sodium (Na) were the predominant minerals. A significant moderate negative correlation was found between altitude and Ca concentration (r = −0.483), alongside a weak negative correlation with Copper (Cu) (r = −0.371). Among the five supervised models tested (Partial Least Squares-Discriminant Analysis (PLS-DA), Random Forest (RF), Support Vector Machine (SVM), Extreme Gradient Boosting (XGBoost), and Artificial Neural Network (ANN)), PLS-DA achieved the highest classification accuracy (94.9%). Variable importance analysis consistently identified Ca as the most influential discriminator across all models, followed by Barium (Ba) and Cu. These minerals, therefore, represent key markers for differentiating honey by geographical origin. This research demonstrates that an integrated model utilizing mineral profiles provides a robust, practical, and reliable method for the geographical authentication of honey. Full article

Flap viability remains a major challenge in reconstructive surgery due to ischemia–reperfusion injury, excessive inflammation, and impaired tissue regeneration. Boron, a trace element with pro-healing and anti-inflammatory properties, has shown therapeutic promise in various wound models; however, its role in flap healing remains unclear. In this study, we aimed to evaluate the therapeutic potential of sodium pentaborate pentahydrate (SPP)-containing hydrogel, a boron compound we developed, for enhancing flap survival and tissue repair. A dorsal random-pattern flap model was established in male Wistar rats, which were treated topically with an SPP-containing formulation twice daily for seven days. Histological changes were evaluated using hematoxylin–eosin and Masson’s trichrome staining, and proteomic alterations were analyzed using label-free nanoLC-MS/MS followed by bioinformatics analysis. The treatment significantly improved flap survival (p < 0.0001), enhanced granulation tissue formation, promoted organized collagen deposition, and reduced inflammatory infiltration. Proteomic profiling identified 179 differentially expressed proteins, with 14 upregulated and 165 downregulated. Upregulated proteins were enriched in pathways related to complement activation, antioxidant defense, and extracellular matrix remodeling, whereas downregulated proteins were associated with immune overactivation, cellular stress, and senescence, indicating a shift toward regulated inflammation and tissue homeostasis. To our knowledge, this is the first study to demonstrate that an SPP-containing hydrogel promotes flap healing by supporting vascularization, modulating immune responses, and enhancing extracellular matrix remodeling. These findings highlight SPP as a promising therapeutic strategy for improving flap viability in reconstructive surgery. Full article

Background/Objectives: Paediatric patients continue to lack access to age-appropriate oral medicines for their treatment and have to depend on the off-label use of medicines approved for adults, which compromises dosing accuracy and exposes children to unpleasant bitterness. Building on previous proof-of-concept work with flucloxacillin sodium, this study investigated the effects of fatty-acid chain length on the formation, stability, dissolution, and sensory acceptability of diclofenac sodium (DS)–Eudragit® EPO (EE)–fatty acid (FA) polyelectrolyte complexes (PECs). Four saturated fatty acids, lauric (C12), myristic (C14), palmitic (C16), and stearic acid (C18), were evaluated at stoichiometric equimolar DS:EE:FA ratio (1:1:1). Methods: PEC microparticles were prepared by solvent evaporation. A stability-indicating RP-HPLC assay was developed and validated according to ICH guidelines to quantify DS content. Drug content and stability were monitored over 3 months at ambient storage. In vitro dissolution was performed in pH 5.5 medium at 37 °C. Taste acceptability and willingness to take again was assessed with 25 healthy adult volunteers using 11-point scale. Results: All PECs retained >90% of expected drug content after 3 months. Compared with neat DS, PECs markedly suppressed early drug release (32–39% vs. 94% at 2 min) but achieved >87% cumulative drug release in 60 min. Sensory evaluation showed significant differences across samples (p < 0.001): neat DS was least acceptable (20.8% willing to take again), while DS-EE-PA was most acceptable (92%), followed by DS-EE-SA and DS-EE-MA. DS-EE-LA was least favoured among PECs. Conclusions: Fatty-acid chain length influenced PEC formation and taste acceptability, but not the PEC stability and drug dissolution profile. Palmitic acid (DS-EE-PA) offered the best overall profile and represents a promising candidate for further development of paediatric-appropriate diclofenac formulations. Full article

This study evaluated the effects of enzymatically produced collagen hydrolysate and Salicornia perennans extract on the quality, oxidative stability, and nutritional composition of canned canine meat pâtés. Two formulations were prepared: a control 2% NaCl, no hydrolysate and an experimental sample containing 3% collagen hydrolysate sheep:camel:bovine = 1:1:1, 1% Salicornia perennans extract, and 1% NaCl. Physicochemical, textural, amino-acid, fatty-acid, and oxidative parameters were monitored over 10 days of storage. The treated pâtés showed similar proximate composition moisture 76.1%, protein 9.2%, metabolizable energy (ME) 102 kcal·100 g⁻¹; p > 0.05 but exhibited enhanced functional stability, with reduced water loss syneresis 1.8 vs. 3.1%; p < 0.05 and improved cohesiveness 0.46 vs. 0.41; p < 0.05. Amino-acid enrichment included higher aspartic acid +33%; p < 0.05, methionine +53%; p < 0.05, and tryptophan +39%; p < 0.05, while the lipid profile showed lower SFA 52.8 vs. 56.4%; p < 0.05, higher n-3 PUFA 1.5 vs. 0.8%; p < 0.05, and a reduced n-6:n-3 ratio 3.8 vs. 5.6; p < 0.05. During storage, oxidative markers decreased: TBARS −45%, carbonyls −14%, acid value −18%, and color stability improved by +2.0 pp. These findings confirm the synergistic antioxidant and structuring effects of collagen-derived peptides and Salicornia polyphenols, as evidenced by a 45% reduction in TBARS, 14% lower protein carbonyls, and 18% lower acid value relative to the control (p < 0.05). This synergy enabled a sodium-reduced, clean-label formulation with improved technological performance, oxidative resistance, and shelf-life stability for functional wet dog foods. In addition, it enhanced the color and visual appeal—key attributes that influence both animal palatability and the purchasing decisions of pet owners. Full article

Background: Irrigation is a key strategy in reducing bioburden, disrupting biofilms, and supporting wound healing. While saline is the standard for its safety and availability, antiseptic and antibiotic solutions are often used in clinical scenarios that require infection control. However, the rise in antibiotic stewardship and concerns regarding cytotoxicity are reshaping current practices. This review identifies recent trends, current controversies, and persistent gaps in knowledge that warrant further investigation and regulatory attention. Methods: A literature review identified irrigation solutions commonly used in plastic surgery; labeling and concentrations were obtained from Devices@FDA, Drugs@FDA, and DailyMed, and PubMed, Cochrane Central, and Embase were searched (January 2022–July 2025) for human studies on acute wounds, chronic wounds, and implant-based breast surgery. Results: In acute wounds, saline and potable tap water effectively prevent infection. In chronic wounds, such as diabetic foot ulcers and pressure injuries, antiseptic agents, including hypochlorous acid, sodium hypochlorite, polyhexanide, and citrate-based solutions, have shown promise in improving healing and reducing infection. In implant-based breast reconstruction and augmentation, data on antiseptics, such as chlorhexidine, and changes in FDA guidance for povidone–iodine and bacitracin have prompted a reevaluation of intraoperative irrigation practices. Conclusion: Despite widespread use, many antiseptics remain off-label, and high-quality clinical studies comparing efficacy and safety are lacking. Full article

The extensive application of fungicides in citrus packinghouses to mitigate economic losses has resulted in the emergence of fungicide-resistant biotypes of Penicillium spp. Furthermore, many countries have implemented strict monitoring of fungicide residues to protect consumer health and the ecosystem. Maximum residue limits (MRLs) have been established in accordance with Codex Alimentarius standards, which present challenges for exports, as exceeding MRLs may restrict market access. This study aimed to identify fungicide-resistant strains of Penicillium spp. in a Moroccan citrus packinghouse and to assess the efficacy of GRAS (Generally Recognized As Safe) salts as eco-friendly alternatives for controlling these resistant strains through in vitro and in vivo tests. A total of 31 Penicillium isolates, labeled H₁ to H₃₁, were collected; 10 were identified as P. digitatum and 21 were identified as P. italicum. Resistance to thiabendazole (61.3%) and imazalil (58.1%) was notable, with some isolates showing dual resistance. In vitro, potassium sorbate, sodium benzoate, and sodium tetraborate salts were highly effective at inhibiting the mycelial growth of resistant isolates, at a concentration of 0.3% (p < 0.0001). In vivo tests on ‘Nadorcott’ fruits demonstrated that 2% and 4% salt solutions effectively prevented the development of green and blue molds caused by Penicillium spp. and showed strong curative effects, resulting in nearly 100% inhibition of most fungal isolates. Additionally, preventive salt treatments increased the accumulation of phenolic and flavonoid compounds, while in fruits treated with sodium benzoate, chitinase and peroxidase activities were significantly enhanced. Full article

Processed and ultra-processed foods (PF/UPFs) are becoming a significant public health concern because of their concerningly high nutrient content, including sodium, and rapidly increasing consumption, especially in low- and middle-income countries. This study aimed to analyze sodium levels in commonly consumed PF/UPFs in Bangladesh, compare them to the WHO’s sodium benchmarks, and assess the discrepancies between label-reported and laboratory-analyzed sodium content. A countrywide cross-sectional survey was conducted among adults, adolescents, and children to identify commonly consumed PFs/UPFs. Most common PF/UPFs were analyzed using Mohr’s titration method to estimate sodium content. Discrepancies between actual content and labels were analyzed, accepting a ±20% deviation, to determine gaps. Among the 974 participants surveyed, PF/UPF consumption in the past week was 97%, with higher consumption among metropolitan and urban residents. Amount of sodium, analyzed in 105 PF/UPF across 14 WHO categories and sub-categories, varied by categories, with soups, instant noodles, and chutneys having the highest. Compliance with the WHO’s sodium benchmarks was inconsistent across categories. The accuracy of label reporting was suboptimal, with under-reporting in 40% of products and a lack of sodium labelling in 9%. These findings unveiled high sodium intake from PFs/UPFs and inaccurate labelling as public health concerns in Bangladesh, highlighting the need for appropriate policies and strategies. Full article

Background: Unhealthy diets are major contributors to obesity and chronic diseases. In 2023, 50.7% of Chinese adults were overweight or obese, underscoring the need to strengthen healthy-eating intentions. Methods: We analysed a cross-sectional online survey of 1145 adults (18–64 years) from Henan and Shandong. Moderation was tested using multiple linear regression with mean-centred interaction terms between each Theory of Planned Behaviour (TPB) construct (attitude, subjective norms, and perceived behavioural control [PBC]) and each food-literacy component (production, choices, preparation and cooking, intake, disposal). Models were adjusted for age, occupation, marital status, alcohol use, physician-diagnosed chronic disease, and living with family. To address multicollinearity, we performed a ridge-regression robustness check (L2-regularised linear model; λ = 0.02 selected by 10-fold cross-validation; CV-RMSE = 0.483; CV-R² = 0.631). We report B, SE, β, p-values, and R²/adjusted R². Results: The overall food-literacy score did not significantly moderate the associations between attitude, subjective norms, or PBC and healthy-eating intention (p = 0.328, 0.671, 0.985). In component-wise analyses, only intake (intake) significantly moderated the PBC–intention association (B = 0.002, SE = 0.001, t = 2.497, p = 0.013); in the ridge model, the effect remained positive (β = 0.182; λ = 0.02). PBC (β = 0.459) and subjective norms (β = 0.169) were the strongest main-effect predictors. The best-fitting model explained R² = 0.663 of the variance in intention (adjusted R² = 0.663). Conclusions: Among adults in Henan and Shandong, the intake component of food literacy strengthened the association between PBC and healthy-eating intention, whereas overall food literacy showed no general moderating effect. Interventions should prioritise intake-related skills (e.g., portion planning, lower-sodium choices and nutrition label use) to enhance perceived behavioural control and, in turn, intention. Given the cross-sectional design, causal inference is limited; longitudinal, capability-building evaluations are warranted. Full article

The objective of this study was to evaluate the potential of clove extract powder (CEP) as a natural curing accelerator in pork sausages produced with pre-converted Chinese cabbage powder (PCCP) as a nitrite source. Sausages were prepared using a 3 × 2 × 2 factorial design with three levels of CEP (0, 500, and 1000 ppm), two sodium ascorbate levels (0 and 500 ppm), and two nitrite sources (synthetic sodium nitrite and PCCP). Increasing the level of CEP decreased pH, CIE L*, CIE a*, and residual nitrite, whereas CIE b*, cured meat pigment, total pigment, and curing efficiency were increased (p < 0.05). The inclusion of sodium ascorbate decreased (p < 0.05) residual nitrite levels while enhancing CIE b*, cured meat pigment, and curing efficiency. Compared with sodium nitrite, PCCP treatments retained higher residual nitrite (p < 0.05), although no significant differences (p ≥ 0.05) were observed for instrumental color, cured meat pigment, total pigment, or curing efficiency. These results demonstrate that CEP, when combined with PCCP, effectively promotes the development of cured meat color and enhances pigment stability, suggesting that this combination can serve as a promising alternative to synthetic nitrite and ascorbate in clean-label pork sausages. Full article