Search Results (1,783)

This study presents the design and microstructural investigation of a single-crystal (SX) Re-bearing high-entropy superalloy (HESA-X1) featuring a thermally stable γ–γ′–γ hierarchical microstructure. The alloy exhibits FCC γ nanoparticles embedded within L1₂-ordered γ′ precipitates, themselves distributed in a γ matrix, with the suppression of detrimental topologically close-packed (TCP) phases. To elucidate solidification behavior and phase stability, Scheil–Gulliver and TC-PRISMA simulations were conducted alongside SEM and XRD analyses. Near-atomic scale analysis in 3D using Atom Probe Tomography (APT) revealed pronounced elemental partitioning, with Re strongly segregating to the γ matrix, while Al and Ti were preferentially enriched in the γ′ phase. Notably, Re demonstrated a unique partitioning behavior compared to conventional superalloys, facilitating the formation and stabilization of γ nanoparticles during two-step aging (Ag-2). These γ nanoparticles significantly contribute to improved mechanical properties. Long-term aging (up to 200 h) at 750–850 °C confirmed exceptional phase stability, with minimal coarsening of γ′ and retention of γ nanoparticles. The coarsening rate constant K of γ′ at 750 °C was significantly lower than that of Re-free HESA, confirming the diffusion-suppressing effect of Re. These findings highlight critical roles of Re in enhancing microstructural stability by reducing atomic mobility, enabling the development of next-generation HESAs with superior thermal and mechanical properties for high-temperature applications. Full article

The study examined the effect of BIE eCoaching on the functional communication training (FCT) implementation skills of pre-service teachers (PTs) and the effect of FCT implemented by PTs on reducing problem behaviors and increasing communication skills of children with autism spectrum disorder (ASD). After the intervention, it was examined whether PTs and children with ASD maintained and generalized the acquired behaviors. Moreover, PTs answered a social validity form before and after the intervention. The methodology of this study involved single-subject research with a multiple-probe design between pairs of participants. The BIE eCoaching intervention was effective in children with ASD’s use, maintenance, and generalization of FCT. FCT was effective in reducing problem behaviors, increasing communication skills, and maintaining and generalizing these skills in children with ASD. Social validity findings showed that PTs’ perceptions of BIE eCoaching changed positively at the end of the intervention. Full article

Mass cytometry has promoted the development of single-cell analysis by enabling the highly multiplexed detection of cellular markers using metal-tagged antibodies or cells. Polymer-based mass cytometry reagents have played a critical role in this technique due to their structural versatility, high metal-loading capacity, and sensitivity. This review comprehensively examines the advances in polymer-based reagents for mass cytometry, focusing on their design principles, synthetic strategies, and biomedical applications. We systematically analyze three key categories: metal-chelating polymers with macrocyclic/acyclic chelators developed through controlled polymerization techniques, polymeric particles including encoded microspheres and semiconducting polymer dots, and emerging metal–organic frameworks with high metal-loading capacities. The discussion highlights how these engineered materials overcome spectral limitations of conventional flow cytometry while addressing current challenges in sensitivity, and multiplexing capacity. Finally, we outline current challenges and future research directions for developing polymer probes in single-cell mass cytometry. Full article

Tumor-associated macrophages (TAMs) are pivotal immune cells within the tumor stroma, whose dynamic alterations significantly impact tumor progression and therapeutic responses. Conventional methods for TAM detection, such as biopsy, are invasive and incapable of whole-body dynamic monitoring. In contrast, positron emission tomography (PET) and single-photon emission computed tomography (SPECT) offer a non-invasive imaging approach by targeting TAM-specific biomarkers like CD206, TSPO, and CCR2. This review comprehensively summarizes the advancements in TAM-targeted imaging probes, including cell surface markers, metabolic/functional markers, and multifunctional nanoprobe, while assessing their potential in tumor immune surveillance and tumor targeting therapeutic applications. While current probes, including ⁶⁸Ga-NOTA-anti-CD206 and ⁶⁴Cu-Macrin, have exhibited high specificity and theragnostic potential in preclinical and early clinical trials, challenges such as target heterogeneity, off-target effects, and clinical translation persist. Moving forward, the advancement of multi-target probes, optimization of pharmacokinetics, and incorporation of multimodal imaging technologies are anticipated to further enhance the impact of TAM-targeted imaging in precision medicine and tumor immunotherapy, fostering the refinement of personalized treatment strategies and improving patient outcomes. Full article

Some pancreatic ductal-type (PDADK) and lung adenocarcinomas (LADK) lacking other molecular drivers are reported to harbor NRG1 fusions as potential novel therapeutic targets. We investigated the feasibility of a fluorescent in situ hybridization (FISH)-based diagnosis of NRG1 fusions in a case series of PDADK and LADK lacking other identified oncogenic drivers. First, among a case series of PDADK, KRAS analyses (PCR followed in PCR-negative cases by RNA sequencing—RNAseq) found 27/162 (16.7%) KRAS wild-type cases, among which 1/162 (0.6%) NRG1 fusion was diagnosed using FISH. Secondly, among a case series of LDAK, 191/446 (42.8%) cases had no molecular alterations in EGFR, KRAS, BRAF, HER2, MET, ALK, ROS1 and RET according to NGS and FISH analyses and, among them, 4/446 (0.9%) cases had NRG1 fusions using FISH. Finally, four additional cases out of the two previously mentioned cases series (1 PDADK and 3 LADK) with NRG1 fusions diagnosed by first-line RNAseq were also concluded as NRG1 FISH-positive. The NRG1 FISH tests for the nine NRG1 FISH-positive cases resulted in 50% to 80% of positive tumor nuclei, all with single 3′-NRG1 FISH signals. In our series, of the 22 cases analyzed with both NRG1 FISH (positivity criterion of at least 15% of tumor nuclei with a split between the 5′- and the 3′- parts of the probes and/or isolated single 3′-NRG1 signal) and RNAseq, 17 cases were FISH– RNAseq– and 5 cases were FISH+ RNAseq+ (no FISH+ RNAseq– or FISH– RNAseq+ cases in our study) resulting in 100% sensibility and specificity for the NRG1 FISH test. In the case of no access to RNAseq, NRG1 FISH consists of a valuable tool searching for NRG1 fusions in patients with advanced cancers. Full article

Oncological hyperthermia (HT) is a medical technique aimed at heating a specific region of the human body containing a tumour. The heat makes the tumour cells more sensitive to the cytotoxic effects of radiotherapy and chemotherapy. Electromagnetic (EM) HT devices radiate a single-frequency EM field that induces a temperature increase in the treated region of the body. The typical radiative HT frequencies are between 60 and 150 MHz for deep HT applications, while 434 MHz and 915 MHz are used for superficial HT. The input EM power can reach up to 2000 W in deep HT and 250 W in superficial applications, and the E-field should be linearly polarized. This study proposes the development and use of E-field sensors to measure the distribution and evaluate the polarization of the E-field radiated by HT devices inside equivalent phantoms. This information is fundamental for the validation and assessment of HT systems. The sensor is constituted by three mutually orthogonal probes. Each probe is composed of a dipole, a diode, and a high-impedance transmission line. The fundamental difference in the operability of this sensor with respect to the standard E-field square-law detectors lies in the high-power values of the considered EM sources. Numerical analyses were performed to optimize the design of the E-field sensor in the whole radiative HT frequency range and to characterize the sensor behaviour at the power levels of HT. Then the sensor was realized, and measurements were carried out to evaluate the E-field radiated by commercial HT systems. The results show the suitability of the developed sensor to measure the E-field radiated by HT applicators. Additionally, in the measured devices, the linear polarization is evidenced. Accordingly, the work shows that in these devices, a single probe can be used to completely characterize the field distribution. Full article

Anticoagulants, including warfarin, are often administered to patients who are exhibiting early symptoms of thromboembolic episodes or who have already experienced such episodes. However, warfarin has a limited therapeutic index and might cause bleeding and other clinical problems. Warfarin inhibits the vitamin K epoxide reductase complex subunit 1 (VKORC1), an enzyme essential for activating vitamin K, in the coagulation cascade. Genetic factors, such as polymorphisms, can change the natural function of VKORC1, causing variations in the medication reaction among individuals. Hence, before prescribing warfarin, the patient’s genetic profile should also be considered. In this study, an electrochemical genosensor capable of detecting the VKORC1 1639 G>A polymorphism was designed and optimized. This analytical approach detects the electric current obtained during the hybridization reaction between two 52 base pair complementary oligonucleotide sequences. Investigating public bioinformatic platforms, two DNA sequences with the A and G single-nucleotide variants were selected and designed. The experimental protocol of the genosensor implied the formation of a bilayer composed of a thiolate DNA and an alkanethiol immobilized onto gold electrodes, as well as the formation of a DNA duplex using a sandwich-format hybridization reaction through a fluorescein labelled DNA signalling probe and the enzymatic amplification of the electrochemical signal, detected by chronoamperometry. A detection limit of 20 pM and a linear range of 0.05–1.00 nM was obtained. A clear differentiation between A/A, G/A and G/G genotypes in biological samples was successfully identified by his novel device. Full article

The Very Long Baseline Interferometry Global Observing System (VGOS), a global network of stations equipped with small-diameter, fast-slewing antennas and broadband receivers, is primarily utilized for geodesy and astrometry. In China, the Shanghai and Urumqi VGOS stations have been developed to perform radio source observation regularly. However, these VGOS stations have not yet been used to observe Earth satellites or deep-space probes. In addition, suitable systems for processing VGOS satellite data are unavailable. In this study, we explored a data processing pipeline and method suitable for VGOS data observed in the dual linear polarization mode and applied to the differential observation of satellites. We present the VGOS observations of the Chang’e 5 lunar orbiter as a pilot experiment for VGOS observations of Earth satellites to verify our processing pipeline. The interferometric fringes were obtained by the cross-correlation of Chang’e 5 lunar orbiter signals. The data analysis yielded a median delay precision of 0.16 ns with 30 s single-channel integration and a baseline closure delay standard deviation of 0.14 ns. The developed data processing pipeline can serve as a foundation for future Earth-orbiting satellite observations, potentially supporting space-tie satellite missions aimed at constructing the terrestrial reference frame (TRF). Full article

In this study, we provide a comparison of radiofrequency electromagnetic field exposure level maps as determined using two approaches: a broadband meter (NARDA EMR-300) equipped with an isotropic probe in the range of 100 kHz to 3 GHz, and a Personal Exposimeter (Satimo EME Spy 140) in the range of 88 MHz to 5.8 GHz. The aim of this research was to determine the necessary adjustments to the measurements made with personal exposimeters to obtain RF-EMF exposure maps equivalent to those made with broadband meters. We evaluated different possibilities to obtain the best equivalence of measurements between both devices. For this purpose, the datasets obtained in both cases were analyzed, as well as the possible correction factors. First, the possibility of establishing a single or double correction factor depending on the existence (or lack thereof) of a line of sight with respect to the base stations was analyzed by minimizing the average value of the error between the values of the broadband meter and the corrected values of the personal exposure meter. Due to the differences observed in the exposure maps, a second procedure was carried out, in which a genetic algorithm was used to determine the ratio between the measurements from both methods (the broadband meter and personal exposure meter), depending on the existence (or lack thereof) of a line of sight, and we compared the exposure maps generated using kriging interpolation. Full article

Background and Clinical Significance: Prader–Willi syndrome (PWS) is a rare genetic disease caused by imprinted gene dysfunction, typically involving deletion of the chromosome 15q11.2-q13 region, balanced translocation, or related gene mutations in this region. PWS presents with complex and varied clinical manifestations. Abnormalities can be observed from the fetal stage and change with age, resulting in growth, developmental, and metabolic issues throughout different life stages. Case Presentation: We report the prenatal characteristics observed from the second to third trimester of pregnancy in a neonate with PWS. Prenatal ultrasound findings included a single umbilical artery, poor abdominal circumference growth from 26 weeks, normal head circumference and femur length growth, increased amniotic fluid volume after 30 weeks, undescended fetal testicles in the third trimester, small kidneys, and reduced fetal movement. The male infant was born at 38 weeks of gestation with a birth weight of 2580 g. He had a weak cry; severe hypotonia; small eyelid clefts; bilateral cryptorchidism; low responsiveness to medical procedures such as blood drawing; and poor sucking, necessitating tube feeding. Blood methylation-specific multiple ligation-dependent probe amplification (MS-MLPA) showed paternal deletion PWS. Notably, this case revealed two previously unreported prenatal features in PWS: a single umbilical artery and small kidneys. Conclusions: Through literature review and our case presentation, we suggest that a combination of specific sonographic features, including these newly identified markers, may aid clinicians in the early diagnosis of PWS. Full article

Five-axis sweep scanning measurement technology, as a novel contact measurement technology, offers excellent reachability and high measurement efficiency for complex parts. However, deviations between the measurement instructions based on the model and the workpiece exist, leading to mismatches between the intended and actual sweep scanning areas, which manifest as displacements of the scanning boundaries and subsequently impact the acquisition of sampling points. When these sampling points are utilized to evaluate the machining quality of workpieces, the accuracy and reliability of the assessment results are compromised. Therefore, by focusing on the phenomenon of boundary displacement in a five-axis sweep scanning measurement, the sampling principle has been analyzed, the constrained sector for the probe tip trajectory in a five-axis scanning measurement has been defined, and the concept of the trajectory constrained sector effect has been proposed for the first time. The constrained sector effect reveals how deviations affect the scanning boundary positions and acquisition of sampling points. Based on the constrained sector effect, the influence of deviations on boundary displacement and sampling point acquisition in single-patch and multiple-patch measurement scenarios is discussed. Furthermore, practical engineering recommendations are provided, aiming to reduce the impact of deviations on the completeness of sampling point acquisition. Full article

Background: Overactive bladder (OAB) has been linked to abnormal cerebral blood flow (CBF) and functional connectivity (FC). However, findings related to CBF and FC changes in OAB remain inconsistent across the literature. Methods: This feasibility study employed arterial spin labeling (ASL) to investigate abnormal CBF and posterior cingulate cortex (PCC) FC in individuals with OAB, both at rest and during bladder filling. ASL images were collected from twenty-two female participants (twelve with OAB and ten healthy controls) at bladder filling volumes of 0, 50, 100, 200, 350, and 500 mL. For OAB participants, scans were obtained both at baseline and following a single-session treatment. ASL images were categorized into low-urge and high-urge conditions based on participants’ subjective urge rating during bladder filling. A flexible factorial design was implemented with three factors: subject, group (control, OAB at baseline, and OAB posttreatment), and urge state (low vs. high). Results: Compared to controls, OAB participants exhibited significant decreases in ΔCBF (high urge minus low urge) in the medial prefrontal cortex and increases in ΔCBF in the supramarginal region. Additionally, ΔPCC FC with the insula was reduced in OAB participants. Posttreatment, OAB participants showed increased ΔPCC FC with the postcentral and parietal (PocP), regions associated with the sensorimotor network. Notably, changes in ΔPCC-PocP FC were associated with improvements in OAB symptoms. Conclusions: These findings support the feasibility of using ASL to probe dysfunctional brain–bladder control mechanisms and treatment-related changes in OAB participants, highlighting the involvement of sensory processing and attention regulation in this condition. Full article

The rapid growth of inverter-based resources (IBRs) has created a need for new islanding detection methodologies to determine whether an IBR has been disconnected from the transmission grid in some manner (islanded) or remains connected to the transmission grid (grid-connected). Active islanding detection methods inject a signal into the power system to achieve detection. Existing schemes frequently limit consideration to a single node system with one IBR. Schemes tested on multiple IBRs often see interference, with the signals from one IBR disturbing the others, or require intricate communication. Further, several methods destabilize an islanded grid to detect it, preventing a prospective microgrid from remaining in operation while islanded. This work develops an active islanding detection scheme using Pulse Compression Probing (PCP) that is microgrid-compatible and can be used with multiple IBRs without requirement for communication. This active islanding detection scheme can be implemented on existing inverter switching sequences and has a detection time of 167–223 ms, well within the detection time specified by existing standards. The method is verified via electromagnetic transient (EMT) simulation on a modified version of a 34-bus test system. Full article

Magnetorheological finishing is widely used in the high-precision processing of optical components, but due to the influence of multi-source system errors, the convergence of single-pass magnetorheological finishing (MRF) is limited. Although iterative processing can improve the surface accuracy, repeated tool paths tend to deteriorate mid-spatial frequency textures, and for complex surfaces such as aspheres, traditional manual alignment is time-consuming and lacks repeatability, significantly restricting the processing efficiency. To address these issues, firstly, this study systematically analyzes the effect of six-degree-of-freedom positioning errors on convergence behavior, establishes a positioning error-normal contour error transmission model, and obtains a workpiece positioning error tolerance threshold that ensures that the relative convergence ratio is not less than 80%. Further, based on these thresholds, a hybrid self-positioning method combining machine vision and a probing module is proposed. A composite data acquisition method using both a camera and probe is designed, and a stepwise global optimization model is constructed by integrating a synchronous iterative localization algorithm with the Non-dominated Sorting Genetic Algorithm II (NSGA-II). The experimental results show that, compared with the traditional alignment, the proposed method improves the convergence ratio of flat workpieces by 41.9% and reduces the alignment time by 66.7%. For the curved workpiece, the convergence ratio is improved by 25.7%, with an 80% reduction in the alignment time. The proposed method offers both theoretical and practical support for high-precision, high-efficiency MRF and intelligent optical manufacturing. Full article

This paper presents a broadband near-field probe designed for measuring the normal magnetic field ($H_z$) in radio frequency (RF) circuits operating within a frequency range of 2–8 GHz. The proposed probe uses a cost-effective 4-layer printed circuit board (PCB) structure made with an FR-4 substrate. The probe primarily consists of an $H_z$ detection unit, a broadband microstrip balun, and a coaxial-like output. The broadband balun facilitates the conversion from differential to single-ended signals, thereby enhancing the probe’s common-mode rejection capability. This design ensures that the probe achieves both cost efficiency and high broadband measurement performance. Additionally, this work investigates the feasibility of employing microstrip lines as calibration standards for the $H_z$ probe. The probe’s structural parameters and magnetic field response were initially determined through simulations, and the calibration factor was subsequently verified by calibration experiments. In practical measurements, the field distributions above a microstrip line and a low-noise amplifier (LNA) were captured. The measured field distribution of the microstrip line was compared with simulation results to verify the probe’s performance. Meanwhile, the measured field distribution of the LNA was utilized to identify the radiating components within the amplifier. Full article