Sign in to use this feature.

Years

Between: -

Subjects

remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline

Journals

remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline

Article Types

Countries / Regions

remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline

Search Results (4,668)

Search Parameters:
Keywords = plasma characterization

Order results
Result details
Results per page
Select all
Export citation of selected articles as:
22 pages, 6337 KiB  
Article
Electrolytic Plasma Hardening of 20GL Steel: Thermal Modeling and Experimental Characterization of Surface Modification
by Bauyrzhan Rakhadilov, Rinat Kurmangaliyev, Yerzhan Shayakhmetov, Rinat Kussainov, Almasbek Maulit and Nurlat Kadyrbolat
Appl. Sci. 2025, 15(15), 8288; https://doi.org/10.3390/app15158288 (registering DOI) - 25 Jul 2025
Abstract
This study investigates the thermal response and surface modification of low-carbon manganese-alloyed 20GL steel during electrolytic plasma hardening. The objective was to evaluate the feasibility of surface hardening 20GL steel—traditionally considered difficult to quench—by combining high-rate surface heating with rapid cooling in an [...] Read more.
This study investigates the thermal response and surface modification of low-carbon manganese-alloyed 20GL steel during electrolytic plasma hardening. The objective was to evaluate the feasibility of surface hardening 20GL steel—traditionally considered difficult to quench—by combining high-rate surface heating with rapid cooling in an electrolyte medium. To achieve this, a transient two-dimensional heat conduction model was developed to simulate temperature evolution in the steel sample under three voltage regimes. The model accounted for dynamic thermal properties and non-linear boundary conditions, focusing on temperature gradients across the thickness. Experimental temperature measurements were obtained using a K-type thermocouple embedded at a depth of 2 mm, with corrections for sensor inertia based on exponential response behavior. A comparison between simulation and experiment was conducted, focusing on peak temperatures, heating and cooling rates, and the effective thermal penetration depth. Microhardness profiling and metallographic examination confirmed surface strengthening and structural refinement, which intensified with increasing voltage. Importantly, the study identified a critical cooling rate threshold of approximately 50 °C/s required to initiate martensitic transformation in 20GL steel. These findings provide a foundation for future optimization of quenching strategies for low-carbon steels by offering insight into the interplay between thermal fluxes, surface kinetics, and process parameters. Full article
(This article belongs to the Section Materials Science and Engineering)
24 pages, 7845 KiB  
Article
Metabolomics and Lipidomics Explore Phenotype-Specific Molecular Signatures for Phenylketonuria
by Buket Yurteri Şahiner, Ali Dursun and Basri Gülbakan
Int. J. Mol. Sci. 2025, 26(15), 7171; https://doi.org/10.3390/ijms26157171 - 25 Jul 2025
Abstract
Phenylketonuria (PKU) is a monogenic disorder caused by pathogenic variants in the gene encoding phenylalanine hydroxylase (PAH), an enzyme essential for phenylalanine (Phe) metabolism. It is characterized by elevated Phe levels, leading to a wide spectrum of clinical phenotypes. These phenotypes are characterized [...] Read more.
Phenylketonuria (PKU) is a monogenic disorder caused by pathogenic variants in the gene encoding phenylalanine hydroxylase (PAH), an enzyme essential for phenylalanine (Phe) metabolism. It is characterized by elevated Phe levels, leading to a wide spectrum of clinical phenotypes. These phenotypes are characterized by varying Phe accumulation, dietary tolerance, and heterogeneous cognitive and neurological outcomes, but current monitoring methods, focused primarily on blood Phe levels, are limited in capturing this variability. In this study, we applied mass spectrometry-based advanced quantitative amino acid analyses, untargeted metabolomics, and lipidomics analyses. We examined the plasma metabolite and lipid profiles in a total of 73 individuals with various PKU phenotypes against healthy controls to see how the metabolome and lipidome of the patients change in different phenotypes. We investigated whether novel markers could be associated with metabolic control status. By elucidating the metabolic and lipid fingerprints of PKU’s phenotypic variability, our findings may provide novel insights that could inform the refinement of dietary and pharmacological interventions, thereby supporting the development of more personalized treatment strategies. Full article
(This article belongs to the Section Molecular Biology)
Show Figures

Figure 1

26 pages, 9486 KiB  
Article
StMAPKK1 Enhances Thermotolerance in Potato (Solanum tuberosum L.) by Enhancing Antioxidant Defense and Photosynthetic Efficiency Under Heat Stress
by Xi Zhu, Yasir Majeed, Kaitong Wang, Xiaoqin Duan, Nengkang Guan, Junfu Luo, Haifei Zheng, Huafen Zou, Hui Jin, Zhuo Chen and Yu Zhang
Plants 2025, 14(15), 2289; https://doi.org/10.3390/plants14152289 - 24 Jul 2025
Abstract
The functional role of MAPKK genes in potato (Solanum tuberosum L.) under high-temperature stress remains unexplored, despite their critical importance in stress signaling and yield protection. We characterized StMAPKK1, a novel group D MAPKK localized to plasma membrane/cytoplasm. Quantitative real-time polymerase chain [...] Read more.
The functional role of MAPKK genes in potato (Solanum tuberosum L.) under high-temperature stress remains unexplored, despite their critical importance in stress signaling and yield protection. We characterized StMAPKK1, a novel group D MAPKK localized to plasma membrane/cytoplasm. Quantitative real-time polymerase chain reaction (qRT-PCR) revealed cultivar-specific upregulation in potato (‘Atlantic’ and ‘Desiree’) leaves under heat stress (25 °C, 30 °C, and 35 °C). Transgenic lines overexpressing (OE) StMAPKK1 exhibited elevated antioxidant enzyme activity, including ascorbate peroxidase (APX), catalase (CAT), superoxide dismutase (SOD), and peroxidase (POD), mitigating oxidative damage. Increased proline and chlorophyll accumulation and reduced oxidative stress markers, hydrogen peroxide (H2O2) and malondialdehyde (MDA), indicate improved cellular redox homeostasis. The upregulation of key antioxidant and heat stress-responsive genes (StAPX, StCAT1/2, StPOD12/47, StFeSOD2/3, StMnSOD, StCuZnSOD1/2, StHSFA3 and StHSP20/70/90) strengthened the enzymatic defense system, enhanced thermotolerance, and improved photosynthetic efficiency, with significant improvements in net photosynthetic rate (Pn), transpiration rate (E), and stomatal conductance (Gs) under heat stress (35 °C) in StMAPKK1-OE plants. Superior growth and biomass (plant height, plant and its root fresh and dry weights, and tuber yield) accumulation, confirming the positive role of StMAPKK1 in thermotolerance. Conversely, RNA interference (RNAi)-mediated suppression of StMAPKK1 led to a reduction in enzymatic activity, proline content, and chlorophyll levels, exacerbating oxidative stress. Downregulation of antioxidant-related genes impaired ROS scavenging capacity and declines in photosynthetic efficiency, growth, and biomass, accompanied by elevated H2O2 and MDA accumulation, highlighting the essential role of StMAPKK1 in heat stress adaptation. These findings highlight StMAPKK1’s potential as a key genetic target for breeding heat-tolerant potato varieties, offering a foundation for improving crop resilience in warming climates. Full article
(This article belongs to the Special Issue Cell Physiology and Stress Adaptation of Crops)
Show Figures

Figure 1

18 pages, 1717 KiB  
Article
An Immune Assay to Quantify the Neutralization of Oxidation-Specific Epitopes by Human Blood Plasma
by Marija Jelic, Philipp Jokesch, Olga Oskolkova, Gernot Faustmann, Brigitte M. Winklhofer-Roob, Bernd Ullrich, Jürgen Krauss, Rudolf Übelhart, Bernd Gesslbauer and Valery Bochkov
Antioxidants 2025, 14(8), 903; https://doi.org/10.3390/antiox14080903 - 24 Jul 2025
Abstract
Oxidized phospholipids (OxPLs) are increasingly recognized as biologically active lipids involved in various pathologies. Both exposure to pathogenic factors and the efficacy of protective mechanisms are critical to disease development. In this study, we characterized an immunoassay that quantified the total capacity of [...] Read more.
Oxidized phospholipids (OxPLs) are increasingly recognized as biologically active lipids involved in various pathologies. Both exposure to pathogenic factors and the efficacy of protective mechanisms are critical to disease development. In this study, we characterized an immunoassay that quantified the total capacity of the plasma to degrade or mask OxPLs, thereby preventing their interaction with cells and soluble proteins. OxLDL-coated plates were first incubated with human blood plasma or a control vehicle, followed by an ELISA using a monoclonal antibody specific to oxidized phosphatidylethanolamine. Pretreatment with the diluted blood plasma markedly inhibited mAb binding. The masking assay was optimized by evaluating the buffer composition, the compatibility with various anticoagulants, potential interfering compounds, the kinetic parameters, pre-analytical stability, statistical robustness, and intra- and inter-individual variability. We propose that this masking assay provides a simple immunological approach to assessing protective mechanisms against lipid peroxidation products. Establishing this robust and reproducible method is essential for conducting clinical association studies that explore masking activity as a potential biomarker of the predisposition to a broad range of lipid-peroxidation-related diseases. Full article
(This article belongs to the Special Issue Exploring Biomarkers of Oxidative Stress in Health and Disease)
Show Figures

Figure 1

19 pages, 9770 KiB  
Article
Microstructural Characterization of S355J2 Steel Plate Cut with Plasma in Water-Bed
by Teodor Machedon-Pisu, Mihai Machedon-Pisu and Arthur Olah
Coatings 2025, 15(8), 866; https://doi.org/10.3390/coatings15080866 - 23 Jul 2025
Viewed by 127
Abstract
When processing widely used materials in welded structures such as steels, a surface operation such as plasma cutting applied in the automated Computer Numerical Control (CNC) version can provide technical and economic benefits to the cut components, but the impact on health and [...] Read more.
When processing widely used materials in welded structures such as steels, a surface operation such as plasma cutting applied in the automated Computer Numerical Control (CNC) version can provide technical and economic benefits to the cut components, but the impact on health and environment must be addressed accordingly. In this paper, a plate with a base material made of S355J2 + AR structural steel is cut in 10 pieces with plasma in a water-bed designed and manufactured by the authors in order to mitigate such risks. The surfaces cut in the water-bed are compared to surfaces cut in open air by macroscopic analyses of the edge cut, by microscopic analyses of the cut parts—base material, heat-affected zone, and cut area—and by hardness determinations. The results reveal improvements as a result of plasma cutting in the water-bed: slag reduction, preservation of granulation, transformations in the austenitic temperature zone, and hardness in the heat-affected zone. Compared to a classical cutting procedure such as oxygen flame cutting, the proposed procedure offers a clean alternative and also requires low maintenance. Full article
(This article belongs to the Section Plasma Coatings, Surfaces & Interfaces)
Show Figures

Figure 1

19 pages, 3031 KiB  
Article
Mutational Profiling Detection in FNAC Samples of Different Types of Thyroid Neoplasms Using Targeted NGS
by Riying Liang, Man Luo, Xinhua Yang, Baoming Luo and Rongbin Liu
Cancers 2025, 17(15), 2429; https://doi.org/10.3390/cancers17152429 - 23 Jul 2025
Viewed by 33
Abstract
Background: Thyroid neoplasms exhibit a diverse molecular landscape, and the 2022 WHO classification emphasizes the critical role of molecular profiling in thyroid cancer management; however, comprehensive mutational data from fine-needle aspiration cytology (FNAC) samples using targeted next-generation sequencing (NGS) are still limited, necessitating [...] Read more.
Background: Thyroid neoplasms exhibit a diverse molecular landscape, and the 2022 WHO classification emphasizes the critical role of molecular profiling in thyroid cancer management; however, comprehensive mutational data from fine-needle aspiration cytology (FNAC) samples using targeted next-generation sequencing (NGS) are still limited, necessitating further investigation to guide clinical practice. Purpose: To characterize the mutational landscape of thyroid neoplasms using targeted NGS of FNAC samples and to assess the clinical implications of molecular profiling. Materials and Methods: This retrospective study included 952 patients with thyroid carcinomaneoplasms who underwent surgery at Sun Yat-sen Memorial Hospital from 2021 to 2023. Preoperative ultrasound, FNAC, and targeted NGS were performed. NGS panels covering 18, 88, and pan-cancer genes were used to analyze FNAC samples. Molecular alterations were correlated with clinical and pathological features. Results: The most frequent mutation was BRAFV600E (84.45%), followed by RET (6.41%), BRCA1/2 (4.41%) and RAS (4.41%). Patients were categorized into BRAF-like (830 cases), RAS-like (36 cases), high-risk mutations (25 cases), and other mutations (28 cases). High-risk mutations were associated with older age and larger tumor size. BRAF-like tumors had a higher lymph node metastasis rate (58.77%) compared to RAS-like tumors (33.33%). Tumor mutation burden varied significantly among different thyroid neoplasm subtypes. Conclusions: Molecular profiling using targeted NGS of FNAC samples provides valuable insights into the genetic landscape of thyroid neoplasms and has significant clinical implications for diagnosis and personalized treatment strategies. Further validation with paired tumor and plasma samples is warranted. Full article
(This article belongs to the Section Molecular Cancer Biology)
Show Figures

Figure 1

15 pages, 4368 KiB  
Article
Antibacterial and Antifungal Tannic Acid Coating on Plasma-Activated Titanium Alloy Surface
by Mariusz Winiecki, Magdalena Stepczyńska, Maciej Walczak, Ewelina Soszczyńska, Magdalena Twarużek, Dorota Bociaga, Marek Trzcinski, Marta Michalska-Sionkowska and Krzysztof Moraczewski
Int. J. Mol. Sci. 2025, 26(15), 7051; https://doi.org/10.3390/ijms26157051 - 22 Jul 2025
Viewed by 168
Abstract
Titanium (Ti) alloys, renowned for their exceptional physicochemical properties and high biocompatibility, are widely utilized in orthopedic and dental implants; however, their lack of intrinsic antimicrobial activity significantly increases the risk of implant-associated infections, often leading to severe complications and implant failure. Developing [...] Read more.
Titanium (Ti) alloys, renowned for their exceptional physicochemical properties and high biocompatibility, are widely utilized in orthopedic and dental implants; however, their lack of intrinsic antimicrobial activity significantly increases the risk of implant-associated infections, often leading to severe complications and implant failure. Developing antimicrobial coatings on Ti implants is therefore a promising strategy. In this study, tannic acid (TA) coatings were deposited by immersing Ti alloy surfaces—beforehand activated by low-temperature oxygen plasma—in TA solutions at 2, 5, and 8 wt%. Coatings were characterized by scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), water contact angle (WCA) measurements, and Folin–Ciocalteu release assays, and their cytocompatibility and antimicrobial performance were assessed in vitro. Surface characterization confirmed the formation of uniform TA layers, and WCA measurements indicated enhanced hydrophilicity relative to unmodified Ti (82.0° ± 3.6°), with values decreasing as TA concentration increased (from 35.2° ± 3.2° for 2% TA to 26.6° ± 2.8° for 8% TA). TA release profiles exhibited an initial burst followed by sustained diffusion, with 5% and 8% coatings releasing significantly more TA than 2% coatings. Coatings containing ≥ 5% TA demonstrated bactericidal activity—achieving > 2-log10 reductions—against Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa, and also showed inhibitory effects against Candida albicans. Importantly, all coatings remained cytocompatible with NIH/3T3 fibroblasts, and the released tannic acid hydrolysis products (particularly gallic acid) enhanced their proliferation. These findings indicate that plasma-activated titanium surfaces coated with ≥5 wt% tannic acid impart broad-spectrum antimicrobial efficacy and hold potential to reduce implant-associated infections and improve long-term outcomes in orthopedic and dental applications. Full article
(This article belongs to the Special Issue The Advances in Antimicrobial Biomaterials)
Show Figures

Figure 1

23 pages, 39698 KiB  
Article
Anti-C1q Autoantibody-Binding Engineered scFv C1q-Mimicking Fragment Enhances Disease Progression in Lupus-Prone MRL/lpr Mice
by Silviya Bradyanova, Nikolina Mihaylova, Nikola Ralchev, Alexandra Kapogianni, Ginka Cholakova, Kalina Nikolova-Ganeva, Ivanka Tsacheva and Andrey Tchorbanov
Int. J. Mol. Sci. 2025, 26(15), 7048; https://doi.org/10.3390/ijms26157048 - 22 Jul 2025
Viewed by 81
Abstract
Systemic lupus erythematosus (SLE) is a chronic inflammatory autoimmune disease characterized by tissue damage in multiple organs caused by autoantibodies and the resulting immune complexes. One possible way for complement system contribution to onset of autoimmune disorder could be realized by the impairment [...] Read more.
Systemic lupus erythematosus (SLE) is a chronic inflammatory autoimmune disease characterized by tissue damage in multiple organs caused by autoantibodies and the resulting immune complexes. One possible way for complement system contribution to onset of autoimmune disorder could be realized by the impairment of C1q-mediated apoptotic clearance as part of human homeostasis. The capacity of C1q to bind early apoptotic cells could be decreased or even lost in the presence of anti-C1q antibodies. A monoclonal anti-idiotypic single-chain (scFv) antibody was selected from the phage library Griffin1” to recognize anti-C1q autoantibodies, purified from sera of lupus nephritis patients. Lupus-prone MRL/lpr mice were injected weekly with scFv A1 fragment-binding anti-C1q antibodies. The number of in vitro and ex vivo studies with collected cells, sera, and organs from the treated animals was performed. scFv treatment changed the percentage of different B-, T-, and NK-cell subpopulations as well as plasma cells and plasmablasts in the spleen and bone marrow. An increase in the levels of splenocyte proliferation, anti-C1q antibodies, and the number of plasma cells producing anti-dsDNA and anti-C1q antibodies were also observed in scFv-treated animals. High levels of proteinuria and hematuria combined with unstable levels of IL10 and IFNγ promote the development of severe lupus and shorten the survival of treated MRL/lpr mice. Therapy with the scFv A1 antibody resulted in BCR recognition on the surface of anti-C1q-specific B-cells and had a disease progression effect, enhancing lupus symptoms in the MRL/lpr mouse model of SLE. Full article
(This article belongs to the Section Molecular Biology)
Show Figures

Figure 1

28 pages, 3926 KiB  
Article
Could the Presence of Ferrihydrite in a Riverbed Impacted by Mining Leachates Be Linked to a Reduction in Contamination and Health Indexes?
by Asunción Guadalupe Morales-Mendoza, Ana Karen Ivanna Flores-Trujillo, Luz María Del-Razo, Betsy Anaid Peña-Ocaña, Fanis Missirlis and Refugio Rodríguez-Vázquez
Water 2025, 17(15), 2167; https://doi.org/10.3390/w17152167 - 22 Jul 2025
Viewed by 241
Abstract
Taxco de Alarcón (Mexico) has been affected by mining activities and the presence of potentially toxic elements (PTEs). In this study, water samples from the Acamixtla, Taxco, and San Juan rivers were analyzed using Inductively Coupled Plasma Optical Emission Spectroscopy (ICP-OES) to determine [...] Read more.
Taxco de Alarcón (Mexico) has been affected by mining activities and the presence of potentially toxic elements (PTEs). In this study, water samples from the Acamixtla, Taxco, and San Juan rivers were analyzed using Inductively Coupled Plasma Optical Emission Spectroscopy (ICP-OES) to determine PTE concentrations. Statistical analyses included principal component analysis, Pearson’s correlation, the Pollution Index, and a Health Risk Assessment. Additionally, solid samples from the San Juan River with leachate from the “La Guadalupana” Mine (RSJMG S2.3) were characterized using Fourier Transform Infrared Spectroscopy (FTIR) and Scanning Electron Microscopy (SEM). Identified PTEs included As, Cr, Ni, Zn, Fe, Mn, Cu, Cd, Pb, Se, and Li. Principal component analysis explained 94.8% of the data variance, and Pearson’s correlation revealed significant associations (p < 0.05) among Fe, As, Cu, Cd, Pb, and Zn. The RSJMG S2.3 site exhibited the highest Pollution Index value (8491.56) and the highest health exposure risks. Lower contamination levels at other sites may be attributed to the complexation of PTEs with ferrihydrite, which was identified in the RSJMG S2.3 site through microscopy and infrared analyses. These findings suggest that the in situ formation of ferrihydrite may enhance the adsorption of PTEs, thereby mitigating environmental contamination and potential health risks. Full article
(This article belongs to the Section Water Quality and Contamination)
Show Figures

Figure 1

18 pages, 1166 KiB  
Article
Incorporation of Pork Meat and Blood Plasma Proteins into a Cocoa Cream Matrix: Characterization, Comparison of Functional Properties, and In Vitro Simulated Digestion
by Milica Stožinić, Đurđica Ačkar, Branislav Šojić, Tea Sedlar, Ljiljana Popović, Biljana Pajin, Ivana Flanjak, Maja Bulatović, Jovana Petrović, Ivana Nikolić and Ivana Lončarević
Foods 2025, 14(14), 2547; https://doi.org/10.3390/foods14142547 - 21 Jul 2025
Viewed by 187
Abstract
Consumer requirements for confectionery products have changed significantly over the past decade. These changes are evident in the growing demand for products that are high in protein but lower in energy content and, as a result, the market for these types of products [...] Read more.
Consumer requirements for confectionery products have changed significantly over the past decade. These changes are evident in the growing demand for products that are high in protein but lower in energy content and, as a result, the market for these types of products is expanding. This study compared the chemical composition and functional properties of pork meat protein (MP) and blood plasma protein (BP) and evaluated their incorporation into cocoa cream formulations. Functional properties, such as water-holding capacity (WHC) and oil-holding capacity (OHC), were determined. Essential amino acid profiles were determined using HPLC analysis, and protein digestibility was evaluated both in the native form and after incorporation into the cocoa cream matrix via in vitro enzymatic digestion assays. Additionally, antioxidant activity of the enriched cocoa creams was assessed using the established ABTS assay. Results showed that BP contained a higher proportion of essential amino acids (26.44% of total amino acids), meeting the FAO/WHO recommendations, and exhibited superior digestibility compared to MP. Both proteins demonstrated high WHC and OHC values. The antioxidant potential of BP-enriched cocoa cream further supported its functional benefits. These findings indicate that blood plasma protein is a promising ingredient for enhancing the nutritional and functional quality of cocoa cream products. Full article
(This article belongs to the Section Food Physics and (Bio)Chemistry)
Show Figures

Figure 1

19 pages, 1109 KiB  
Article
Machine Learning Approach to Select Small Compounds in Plasma as Predictors of Alzheimer’s Disease
by Eleonora Stefanini, Alberto Iglesias, Joan Serrano-Marín, Juan Sánchez-Navés, Hanan A. Alkozi, Mercè Pallàs, Christian Griñán-Ferré, David Bernal-Casas and Rafael Franco
Int. J. Mol. Sci. 2025, 26(14), 6991; https://doi.org/10.3390/ijms26146991 - 21 Jul 2025
Viewed by 119
Abstract
This study employs a machine learning approach to identify a small-molecule-based signature capable of predicting Alzheimer’s disease (AD). Utilizing metabolomics data from the plasma of a well-characterized cohort of 94 AD patients and 62 healthy controls; metabolite levels were assessed using the Biocrates [...] Read more.
This study employs a machine learning approach to identify a small-molecule-based signature capable of predicting Alzheimer’s disease (AD). Utilizing metabolomics data from the plasma of a well-characterized cohort of 94 AD patients and 62 healthy controls; metabolite levels were assessed using the Biocrates MxP® Quant 500 platform. Data preprocessing involved removing low-quality samples, selecting relevant biochemical groups, and normalizing metabolite data based on demographic variables such as age, sex, and fasting time. Linear regression models were used to identify concomitant parameters that consisted of the data for a given metabolite within each of the biochemical families that were considered. Detection of these “concomitant” metabolites facilitates normalization and allows sample comparison. Residual analysis revealed distinct metabolite profiles between AD patients and controls across groups, such as amino acid-related compounds, bile acids, biogenic amines, indoles, carboxylic acids, and fatty acids. Correlation heatmaps illustrated significant interdependencies, highlighting specific molecules like carnosine, 5-aminovaleric acid (5-AVA), cholic acid (CA), and indoxyl sulfate (Ind-SO4) as promising indicators. Linear Discriminant Analysis (LDA), validated using Leave-One-Out Cross-Validation, demonstrated that combinations of four or five molecules could classify AD with accuracy exceeding 75%, sensitivity up to 80%, and specificity around 79%. Notably, optimal combinations integrated metabolites with both a tendency to increase and a tendency to decrease in AD. A multivariate strategy consistently identified included 5-AVA, carnosine, CA, and hypoxanthine as having predictive potential. Overall, this study supports the utility of combining data of plasma small molecules as predictors for AD, offering a novel diagnostic tool and paving the way for advancements in personalized medicine. Full article
(This article belongs to the Section Molecular Neurobiology)
Show Figures

Figure 1

27 pages, 3394 KiB  
Article
Integrative Multi-Omics Profiling of Rhabdomyosarcoma Subtypes Reveals Distinct Molecular Pathways and Biomarker Signatures
by Aya Osama, Ahmed Karam, Abdelrahman Atef, Menna Arafat, Rahma W. Afifi, Maha Mokhtar, Taghreed Khaled Abdelmoneim, Asmaa Ramzy, Enas El Nadi, Asmaa Salama, Emad Elzayat and Sameh Magdeldin
Cells 2025, 14(14), 1115; https://doi.org/10.3390/cells14141115 - 20 Jul 2025
Viewed by 205
Abstract
Rhabdomyosarcoma (RMS), the most common pediatric soft tissue sarcoma, comprises embryonal (ERMS) and alveolar (ARMS) subtypes with distinct histopathological features, clinical outcomes, and therapeutic responses. To better characterize their molecular distinctions, we performed untargeted plasma proteomics and metabolomics profiling in children with ERMS [...] Read more.
Rhabdomyosarcoma (RMS), the most common pediatric soft tissue sarcoma, comprises embryonal (ERMS) and alveolar (ARMS) subtypes with distinct histopathological features, clinical outcomes, and therapeutic responses. To better characterize their molecular distinctions, we performed untargeted plasma proteomics and metabolomics profiling in children with ERMS (n = 18), ARMS (n = 17), and matched healthy controls (n = 18). Differential expression, functional enrichment (GO, KEGG, RaMP-DB), co-expression network analysis (WGCNA/WMCNA), and multi-omics integration (DIABLO, MOFA) revealed distinct molecular signatures for each subtype. ARMS displayed elevated oncogenic and stemness-associated proteins (e.g., cyclin E1, FAP, myotrophin) and metabolites involved in lipid transport, fatty acid metabolism, and polyamine biosynthesis. In contrast, ERMS was enriched in immune-related and myogenic proteins (e.g., myosin-9, SAA2, S100A11) and metabolites linked to glutamate/glycine metabolism and redox homeostasis. Pathway analyses highlighted subtype-specific activation of PI3K-Akt and Hippo signaling in ARMS and immune and coagulation pathways in ERMS. Additionally, the proteomics and metabolomics datasets showed association with clinical parameters, including disease stage, lymph node involvement, and age, demonstrating clear molecular discrimination consistent with clinical observation. Co-expression networks and integrative analyses further reinforced these distinctions, uncovering coordinated protein–metabolite modules. Our findings reveal novel, subtype-specific molecular programs in RMS and propose candidate biomarkers and pathways that may guide precision diagnostics and therapeutic targeting in pediatric sarcomas. Full article
Show Figures

Figure 1

21 pages, 3324 KiB  
Article
Curcumin and Papain-Loaded Liposomal Natural Latex Dressings with Phototherapy: A Synergistic Approach to Diabetic Wound Healing
by Franciéle M. Silva, Jaqueline R. Silva, Wellington Rodrigues, Breno A. S. M. Sousa, Thamis F. S. Gomes, Mario F. F. Rosa, Suélia S. R. F. Rosa and Marcella L. B. Carneiro
Pharmaceuticals 2025, 18(7), 1067; https://doi.org/10.3390/ph18071067 - 20 Jul 2025
Viewed by 284
Abstract
Background: Wound healing in diabetic individuals is a prolonged process, often complicated by infections and impaired tissue regeneration. Innovative strategies combining natural bioactive compounds are needed to enhance repair. Methods: This study reports the development and characterization of natural latex-based biomembranes (NLBs) incorporated [...] Read more.
Background: Wound healing in diabetic individuals is a prolonged process, often complicated by infections and impaired tissue regeneration. Innovative strategies combining natural bioactive compounds are needed to enhance repair. Methods: This study reports the development and characterization of natural latex-based biomembranes (NLBs) incorporated with liposome-encapsulated curcumin and papain. The therapeutic efficacy of these composite dressings, in combination with red light-emitting diode (LED) phototherapy, was evaluated in a diabetic rat model. NLBs were produced by blending natural latex with multilamellar liposomes containing either curcumin, papain, or both. In vivo wound healing was assessed by applying the biomembranes to the dorsal lesions and administering red LED irradiation (650 ± 20 nm, 10 min every 48 h) over 11 days. Results: Fourier transform infrared spectroscopy (FTIR) confirmed that the integration of liposomes did not induce significant chemical alterations to the latex matrix. The treated diabetic rats exhibited enhanced wound contraction, with the curcumin and papain groups demonstrating up to 99% and 95% healing, respectively. Plasma fructosamine levels were significantly reduced (p < 0.05), indicating improved glycemic control. Conclusions: Combining NLBs with bioactive-loaded liposomes and phototherapy accelerated wound healing in diabetic rats. This multifunctional platform shows promise for the treatment of chronic wounds in diabetic patients. Full article
(This article belongs to the Section Pharmacology)
Show Figures

Graphical abstract

9 pages, 252 KiB  
Article
On Extended d-D Kappa Distribution
by Arak M. Mathai and Hans J. Haubold
Axioms 2025, 14(7), 539; https://doi.org/10.3390/axioms14070539 - 17 Jul 2025
Viewed by 95
Abstract
The thermal Doppler broadening of spectral profiles for particle populations in the absence or presence of potential fields can be described by kappa distributions. The kappa distribution provides a replacement for the Maxwell–Boltzmann distribution, which can be considered as a generalization for describing [...] Read more.
The thermal Doppler broadening of spectral profiles for particle populations in the absence or presence of potential fields can be described by kappa distributions. The kappa distribution provides a replacement for the Maxwell–Boltzmann distribution, which can be considered as a generalization for describing systems characterized by local correlations among their particles, as found in space and astrophysical plasmas. This paper presents all special cases of kappa distributions as members of a general pathway family of densities introduced by Mathai. The aim of the present paper is to bring to attention the application of various forms of the kappa distribution, its various special cases and its generalizations, which, in scalar-variable and multivariate situations, belong to a general family of distributions known as Mathai’s pathway models, comprising three different families of functions, namely the generalized type-1 beta, type-2 beta and gamma families. Through one parameter, known as the pathway parameter, one will be able to reach all the three families of functions and the stages of transitioning from one family to another. After pointing out the connection of multivariate (vector-variate) kappa distributions to the multivariate pathway model, the multivariate kappa distribution is extended to the real matrix-variate case by working out the various forms and by evaluating the normalizing constants of the various forms of the matrix-variate case explicitly. It is also pointed out that the pathway models are available for the scalar, vector and rectangular matrix-variate cases in the real domain as well as in the complex domain. Full article
32 pages, 16657 KiB  
Article
Meta-Analysis of Gene Expression in Bulk-Processed Post-Mortem Spinal Cord from ALS Patients and Normal Controls
by William R. Swindell
NeuroSci 2025, 6(3), 65; https://doi.org/10.3390/neurosci6030065 - 16 Jul 2025
Viewed by 458
Abstract
Amyotrophic lateral sclerosis (ALS) is characterized by upper and lower motor neuron failure and poor prognosis. This study performed a meta-analysis of gene expression datasets that compared bulk-processed post-mortem spinal cord from ALS and control (CTL) patients. The analysis included 569 samples (454 [...] Read more.
Amyotrophic lateral sclerosis (ALS) is characterized by upper and lower motor neuron failure and poor prognosis. This study performed a meta-analysis of gene expression datasets that compared bulk-processed post-mortem spinal cord from ALS and control (CTL) patients. The analysis included 569 samples (454 ALS, 115 CTL) from 348 individuals (262 ALS, 86 CTL). Patterns of differential expression bias, related to mRNA abundance, gene length and GC content, were discernable from individual studies but attenuated by meta-analysis. A total of 213 differentially expressed genes (DEGs) were identified (144 ALS-increased, 69 ALS-decreased). ALS-increased DEGs were most highly expressed by microglia and associated with MHC class II, immune response and leukocyte activation. ALS-decreased DEGs were abundantly expressed by mature oligodendrocytes (e.g., the MOL5 phenotype) and associated with myelin production, plasma membrane and sterol metabolism. Comparison to spatial transcriptomics data showed that DEGs were prominently expressed in white matter, with increased DEG expression strongest in the ventral/lateral white matter. These results highlight white matter as the spinal cord region most strongly associated with the shifts in mRNA abundance observed in bulk-processed tissues. These shifts can be explained by attrition of mature oligodendrocytes and an ALS-emergent microglia phenotype that is partly shared among neurodegenerative conditions. Full article
Show Figures

Graphical abstract

Back to TopTop