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11 pages, 638 KiB  
Communication
Millet in Bioregenerative Life Support Systems: Hypergravity Resilience and Predictive Yield Models
by Tatiana S. Aniskina, Arkady N. Kudritsky, Olga A. Shchuklina, Nikita E. Andreev and Ekaterina N. Baranova
Life 2025, 15(8), 1261; https://doi.org/10.3390/life15081261 (registering DOI) - 7 Aug 2025
Abstract
The prospects for long-distance space flights are becoming increasingly realistic, and one of the key factors for their implementation is the creation of sustainable systems for producing food on site. Therefore, the aim of our work is to assess the prospects for using [...] Read more.
The prospects for long-distance space flights are becoming increasingly realistic, and one of the key factors for their implementation is the creation of sustainable systems for producing food on site. Therefore, the aim of our work is to assess the prospects for using millet in biological life support systems and to create predictive models of yield components for automating plant cultivation control. The study found that stress from hypergravity (800 g, 1200 g, 2000 g, and 3000 g) in the early stages of millet germination does not affect seedlings or yield. In a closed system, millet yield reached 0.31 kg/m2, the weight of 1000 seeds was 8.61 g, and the yield index was 0.06. The paper describes 40 quantitative traits, including six leaf and trichome traits and nine grain traits from the lower, middle and upper parts of the inflorescence. The compiled predictive regression equations allow predicting the accumulation of biomass in seedlings on the 10th and 20th days of cultivation, as well as the weight of 1000 seeds, the number of productive inflorescences, the total above-ground mass, and the number and weight of grains per plant. These equations open up opportunities for the development of computer vision and high-speed plant phenotyping programs that will allow automatic correction of the plant cultivation process and modeling of the required yield. Predicting biomass yield will also be useful in assessing the load on the waste-free processing system for plant waste at planetary stations. Full article
(This article belongs to the Special Issue Physiological Responses of Plants Under Abiotic Stresses)
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14 pages, 706 KiB  
Article
Study on the Effects of Irrigation Amount on Spring Maize Yield and Water Use Efficiency Under Different Planting Patterns in Xinjiang
by Ruxiao Bai, Haixiu He, Xinjiang Zhang and Qifeng Wu
Agriculture 2025, 15(15), 1710; https://doi.org/10.3390/agriculture15151710 (registering DOI) - 7 Aug 2025
Abstract
Planting patterns and irrigation amounts are key factors affecting maize yield. This study adopted a two-factor experimental design, with planting pattern as the main plot and irrigation amount as the subplot, to investigate the effects of irrigation levels under different planting patterns (including [...] Read more.
Planting patterns and irrigation amounts are key factors affecting maize yield. This study adopted a two-factor experimental design, with planting pattern as the main plot and irrigation amount as the subplot, to investigate the effects of irrigation levels under different planting patterns (including uniform row spacing and alternating wide-narrow row spacing) on spring maize yield and water use efficiency in Xinjiang. Through this approach, the study examined the mechanisms by which planting pattern and irrigation amount influence maize growth, yield formation, and water use efficiency. Experiments conducted at the Agricultural Science Research Institute of the Ninth Division of Xinjiang Production and Construction Corps demonstrated that alternating wide-narrow row spacing combined with moderate irrigation (5400 m3/hm2) significantly optimized maize root distribution, improved water use efficiency, and increased leaf area index and net photosynthetic rate, thereby promoting dry matter accumulation and yield enhancement. In contrast, uniform row spacing under high irrigation levels increased yield but resulted in lower water use efficiency. The study also found that alternating wide-narrow row spacing enhanced maize nutrient absorption from the soil, particularly phosphorus utilization efficiency, by improving canopy structure and root expansion. This pattern exhibited comprehensive advantages in resource utilization, providing a theoretical basis and technical pathway for achieving water-saving and high-yield maize production in arid regions, which holds significant importance for promoting sustainable agricultural development. Full article
(This article belongs to the Section Crop Production)
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10 pages, 462 KiB  
Article
Mutation Rates and Fitness Genes in Staphylococcus aureus Treated with the Medicinal Plant Synadenium glaucescens
by Zaituni Msengwa, Martin Saxtorph Bojer, Frank Rwegoshora, James Mwesongo, Magesa Mafuru, Faith Philemon Mabiki, Beda John Mwang’onde, Madundo Mkumbukwa Mtambo, Lughano Jeremy Kusiluka, Henrik Christensen, Robinson Hammerthon Mdegela and John Elmerdahl Olsen
Appl. Sci. 2025, 15(15), 8753; https://doi.org/10.3390/app15158753 (registering DOI) - 7 Aug 2025
Abstract
Extracts, fractions and the pure compound epifriedelanol of the medicinal plant Synadenium glaucescens have antibacterial properties. Herbal products are generally considered less prone to resistance development than conventional antimicrobials, as they contain multiple compounds, which makes bacteria less likely to develop resistance. However, [...] Read more.
Extracts, fractions and the pure compound epifriedelanol of the medicinal plant Synadenium glaucescens have antibacterial properties. Herbal products are generally considered less prone to resistance development than conventional antimicrobials, as they contain multiple compounds, which makes bacteria less likely to develop resistance. However, data supporting this notion are lacking. This study evaluated the development of resistance in Staphylococcus aureus subjected to extract, fractions and epifriedelanol of S. glaucescens. It also identified S. aureus fitness genes contributing to intrinsic resistance to extract of S. glaucescens. Fluctuation and gradient concentration assays were used to determine mutation rates and growth adaptation, respectively, which were lower following exposure to growth in crude extract than the pure compound epifriedelanol. By subjecting 1920 single gene mutants from the Nebraska Transposon Mutant Library to growth in the presence of extract of S. glaucescens, 12 genes were identified as important for natural resistance in S. aureus JE2; however, only mutation in the hemB gene decreased the minimum inhibitory concentration by greater than 4-fold (64-fold). In conclusion, purifying active antimicrobial compounds from S. glaucescens and using them as antibacterial substances as an alternative to crude extract increased the risk of resistance development. Further, the gene hemB appears to have a significant role in the natural resistance to the extracts obtained from S. glaucescens in this study. Full article
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21 pages, 2047 KiB  
Article
Sustainable Management of Fruit By-Products Through Design Thinking: Development of an Innovative Food Product
by Sylwia Sady, Alfred Błaszczyk, Bogdan Pachołek, Anna Muzykiewicz-Szymańska, Anna Nowak, Justyna Syguła-Cholewińska, Tomasz Sawoszczuk, Stanisław Popek, Małgorzata Krzywonos, Agnieszka Piekara and Dominika Jakubowska
Sustainability 2025, 17(15), 7164; https://doi.org/10.3390/su17157164 (registering DOI) - 7 Aug 2025
Abstract
Sustainable development and the circular economy have become key challenges in the modern food sector, calling for innovative solutions that reduce waste and promote the efficient use of resources. The aim of this study was to develop a functional food product by utilizing [...] Read more.
Sustainable development and the circular economy have become key challenges in the modern food sector, calling for innovative solutions that reduce waste and promote the efficient use of resources. The aim of this study was to develop a functional food product by utilizing by-products from chokeberry processing, thereby contributing to circularity in food systems. The integration of design thinking with fermentation of chokeberry pomace is presented in this study as an approach to developing value-added food ingredients. Qualitative consumer research (focus group interviews, n = 36) identified preferences and expectations regarding functional foods containing by-products. Conducted by an interdisciplinary team, the project followed five stages, involving both qualitative and quantitative research. Liquid surface fermentation was performed using Aspergillus niger, selected for its proven ability to enhance the antioxidant capacity and polyphenol content of plant matrices. The optimal process was 2-day fermentation under controlled pH conditions with glucose supplementation, which significantly enhanced the quality and nutritional value of the final product. Antioxidant activity (ABTS, FRAP, CUPRAC assays), total polyphenols, anthocyanins, and proanthocyanidins were determined, showing significant increases compared to non-fermented controls. The outcome was the development of a dried, fermented chokeberry pomace product that meets consumer expectations and fulfils sustainability goals through waste reduction and innovative reuse of fruit processing by-products. Full article
(This article belongs to the Special Issue Innovative Technologies in Food Engineering Towards Sustainability)
20 pages, 1149 KiB  
Article
Assessment of Biomethane Potential from Waste Activated Sludge in Swine Wastewater Treatment and Its Co-Digestion with Swine Slurry, Water Lily, and Lotus
by Sartika Indah Amalia Sudiarto, Hong Lim Choi, Anriansyah Renggaman and Arumuganainar Suresh
AgriEngineering 2025, 7(8), 254; https://doi.org/10.3390/agriengineering7080254 (registering DOI) - 7 Aug 2025
Abstract
Waste activated sludge (WAS), a byproduct of livestock wastewater treatment, poses significant disposal challenges due to its low biodegradability and potential environmental impact. Anaerobic digestion (AD) offers a sustainable approach for methane recovery and sludge stabilization. This study evaluates the biomethane potential (BMP) [...] Read more.
Waste activated sludge (WAS), a byproduct of livestock wastewater treatment, poses significant disposal challenges due to its low biodegradability and potential environmental impact. Anaerobic digestion (AD) offers a sustainable approach for methane recovery and sludge stabilization. This study evaluates the biomethane potential (BMP) of WAS and its co-digestion with swine slurry (SS), water lily (Nymphaea spp.), and lotus (Nelumbo nucifera) shoot biomass to enhance methane yield. Batch BMP assays were conducted at substrate-to-inoculum (S/I) ratios of 1.0 and 0.5, with methane production kinetics analyzed using the modified Gompertz model. Mono-digestion of WAS yielded 259.35–460.88 NmL CH4/g VSadded, while co-digestion with SS, water lily, and lotus increased yields by 14.89%, 10.97%, and 16.89%, respectively, surpassing 500 NmL CH4/g VSadded. All co-digestion combinations exhibited synergistic effects (α > 1), enhancing methane production beyond individual substrate contributions. Lower S/I ratios improved methane yields and biodegradability, highlighting the role of inoculum availability. Co-digestion reduced the lag phase limitations of WAS and plant biomass, improving process efficiency. These findings demonstrate that co-digesting WAS with nutrient-rich co-substrates optimizes biogas production, supporting sustainable sludge management and renewable energy recovery in livestock wastewater treatment systems. Full article
(This article belongs to the Section Sustainable Bioresource and Bioprocess Engineering)
20 pages, 3766 KiB  
Review
Challenges, Unmet Needs, and Future Directions for Nanocrystals in Dermal Drug Delivery
by Muzn Alkhaldi and Cornelia M. Keck
Molecules 2025, 30(15), 3308; https://doi.org/10.3390/molecules30153308 - 7 Aug 2025
Abstract
Nanocrystals, defined as crystalline particles with dimensions in the nanometer range (<1000 nm), exhibit unique properties that enhance the efficacy of poorly soluble active compounds. This review explores the fundamental aspects of nanocrystals, including their characteristics and various preparation methods, while addressing critical [...] Read more.
Nanocrystals, defined as crystalline particles with dimensions in the nanometer range (<1000 nm), exhibit unique properties that enhance the efficacy of poorly soluble active compounds. This review explores the fundamental aspects of nanocrystals, including their characteristics and various preparation methods, while addressing critical factors that influence their stability and incorporation into final products. A key focus of the review is the advantages offered by nanocrystals in dermal applications. It also highlights their ability to enhance passive diffusion into the skin and facilitate penetration via particle-assisted dermal penetration. Additionally, the review discusses their capacity to penetrate into hair follicles, enabling targeted drug delivery, and their synergistic potential when combined with microneedles, which further enhance the dermal absorption of active compounds. The review also addresses several commercial products that successfully employ nanocrystal technology, showcasing its practical applications. Summary: Nanocrystals with their special properties are an emerging trend for dermal applications, particularly the development of plantCrystals—natural nanocrystals sourced from plant materials—which represent a promising path for future research and formulation strategies. These advancements could lead to more sustainable and effective dermal products. Full article
(This article belongs to the Section Natural Products Chemistry)
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34 pages, 3764 KiB  
Review
Research Progress and Applications of Artificial Intelligence in Agricultural Equipment
by Yong Zhu, Shida Zhang, Shengnan Tang and Qiang Gao
Agriculture 2025, 15(15), 1703; https://doi.org/10.3390/agriculture15151703 - 7 Aug 2025
Abstract
With the growth of the global population and the increasing scarcity of arable land, traditional agricultural production is confronted with multiple challenges, such as efficiency improvement, precision operation, and sustainable development. The progressive advancement of artificial intelligence (AI) technology has created a transformative [...] Read more.
With the growth of the global population and the increasing scarcity of arable land, traditional agricultural production is confronted with multiple challenges, such as efficiency improvement, precision operation, and sustainable development. The progressive advancement of artificial intelligence (AI) technology has created a transformative opportunity for the intelligent upgrade of agricultural equipment. This article systematically presents recent progress in computer vision, machine learning (ML), and intelligent sensing. The key innovations are highlighted in areas such as object detection and recognition (e.g., a K-nearest neighbor (KNN) achieved 98% accuracy in distinguishing vibration signals across operation stages); autonomous navigation and path planning (e.g., a deep reinforcement learning (DRL)-optimized task planner for multi-arm harvesting robots reduced execution time by 10.7%); state perception (e.g., a multilayer perceptron (MLP) yielded 96.9% accuracy in plug seedling health classification); and precision control (e.g., an intelligent multi-module coordinated control system achieved a transplanting efficiency of 5000 plants/h). The findings reveal a deep integration of AI models with multimodal perception technologies, significantly improving the operational efficiency, resource utilization, and environmental adaptability of agricultural equipment. This integration is catalyzing the transition toward intelligent, automated, and sustainable agricultural systems. Nevertheless, intelligent agricultural equipment still faces technical challenges regarding data sample acquisition, adaptation to complex field environments, and the coordination between algorithms and hardware. Looking ahead, the convergence of digital twin (DT) technology, edge computing, and big data-driven collaborative optimization is expected to become the core of next-generation intelligent agricultural systems. These technologies have the potential to overcome current limitations in perception and decision-making, ultimately enabling intelligent management and autonomous decision-making across the entire agricultural production chain. This article aims to provide a comprehensive foundation for advancing agricultural modernization and supporting green, sustainable development. Full article
(This article belongs to the Section Agricultural Technology)
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21 pages, 961 KiB  
Article
A Mixed-Method Assessment of Drivers and Barriers for Substituting Dairy with Plant-Based Alternatives by Danish Adults
by Beatriz Philippi Rosane, Lise Tjørring, Annika Ley, Derek Victor Byrne, Barbara Vad Andersen, Susanne Gjedsted Bügel and Sophie Wennerscheid
Foods 2025, 14(15), 2755; https://doi.org/10.3390/foods14152755 - 7 Aug 2025
Abstract
The market for plant-based alternatives to animal foods has increased rapidly in the past decade, mainly due to consumer demand. Little evidence is available regarding nutritional impacts, drivers, and barriers to using these products as substitutes for animal foods in real-life conditions. This [...] Read more.
The market for plant-based alternatives to animal foods has increased rapidly in the past decade, mainly due to consumer demand. Little evidence is available regarding nutritional impacts, drivers, and barriers to using these products as substitutes for animal foods in real-life conditions. This pilot study followed 16 Danish adults (30 ± 11 years old; 11 females) for 4 weeks with substituting milk, cheese, and yogurt with plant-based analogues to dairy (PBADs) and assessed their drivers and barriers to applying the intervention with a mixed-method approach. PBADs are constantly compared to their animal counterparts, both regarding product characteristics, such as price and sensory properties, as well as cultural roles and subjective memories. The mixed methods showed dairy attachment, price, and taste were the main barriers to consuming PBAD, while changes in life and social circles were drivers (qualitative data). As for the liking of PBADs, plant-based yoghurt was the preferred intervention product (73.5/100, p < 0.05), followed by plant-based drinks (65.9/100), while plant-based cheese was the lowest rated (47.9/100, p < 0.05). As for dietary changes, a lower average intake of sugars, saturated fatty acids, cholesterol, calcium, phosphorus, and zinc was observed after the intervention. Additionally, this study describes the attachment of the study population to milk and dairy products. It shows that choosing dairy is beyond nourishment but is connected to tradition, culture, pleasure, memories, and a sense of belonging. In contrast, there is no history or attachment to PBADs. Full article
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32 pages, 5466 KiB  
Article
Comprehensive Energy and Economic Analysis of Selected Variants of a Large-Scale Photovoltaic Power Plant in a Temperate Climate
by Dennis Thom, Artur Bugała, Dorota Bugała and Wojciech Czekała
Energies 2025, 18(15), 4198; https://doi.org/10.3390/en18154198 - 7 Aug 2025
Abstract
In recent years, solar energy has emerged as one of the most advanced renewable energy sources, with its production capacity steadily growing. To maximize output and efficiency, choosing the right configuration for a specific location for these installations is crucial. This study uniquely [...] Read more.
In recent years, solar energy has emerged as one of the most advanced renewable energy sources, with its production capacity steadily growing. To maximize output and efficiency, choosing the right configuration for a specific location for these installations is crucial. This study uniquely integrates detailed multi-variant fixed-tilt PV system simulations with comprehensive economic evaluation under temperate climate conditions, addressing site-specific spatial constraints and grid integration considerations that have rarely been combined in previous works. In this paper, an energy and economic efficiency analysis for a photovoltaic power plant, located in central Poland, designed in eight variants (10°, 15°, 20°, 25°, 30° PV module inclination angle for a south orientation and 10°, 20°, 30° for an east–west orientation) for a limited building area of approximately 300,000 m2 was conducted. In PVSyst computer simulations, PVGIS-SARAH2 solar radiation data were used together with the most common data for describing the Polish local solar climate, called Typical Meteorological Year data (TMY). The most energy-efficient variants were found to be 20° S and 30° S, configurations with the highest surface production coefficient (249.49 and 272.68 kWh/m2) and unit production efficiency values (1123 and 1132 kWh/kW, respectively). These findings highlight potential efficiency gains of up to approximately 9% in surface production coefficient and financial returns exceeding 450% ROI, demonstrating significant economic benefits. In economic terms, the 15° S variant achieved the highest values of financial parameters, such as the return on investment (ROI) (453.2%), the value of the average annual share of profits in total revenues (56.93%), the shortest expected payback period (8.7 years), the value of the levelized cost of energy production (LCOE) (0.1 EUR/kWh), and one of the lowest costs of building 1 MWp of a photovoltaic farm (664,272.7 EUR/MWp). Among the tested variants of photovoltaic farms with an east–west geographical orientation, the most advantageous choice is the 10° EW arrangement. The results provide valuable insights for policymakers and investors aiming to optimize photovoltaic deployment in temperate climates, supporting the broader transition to renewable energy and alignment with national energy policy goals. Full article
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18 pages, 2972 KiB  
Article
Flavonoids from Cercidiphyllum japonicum Exhibit Bioactive Potential Against Skin Aging and Inflammation in Human Dermal Fibroblasts
by Minseo Kang, Sanghyun Lee, Dae Sik Jang, Sullim Lee and Daeyoung Kim
Curr. Issues Mol. Biol. 2025, 47(8), 631; https://doi.org/10.3390/cimb47080631 - 7 Aug 2025
Abstract
With increasing interest in natural therapeutic strategies for skin aging, plant-derived compounds have gained attention for their potential to protect against oxidative stress and inflammation. In this study, we investigated the anti-aging and anti-inflammatory effects of flavonoids isolated from Cercidiphyllum japonicum using a [...] Read more.
With increasing interest in natural therapeutic strategies for skin aging, plant-derived compounds have gained attention for their potential to protect against oxidative stress and inflammation. In this study, we investigated the anti-aging and anti-inflammatory effects of flavonoids isolated from Cercidiphyllum japonicum using a tumor necrosis factor-alpha (TNF-α)-stimulated normal human dermal fibroblast (NHDF) model. The aerial parts of C. japonicum were extracted and analyzed by high-performance liquid chromatography (HPLC), leading to the identification of four major compounds: maltol, chlorogenic acid, ellagic acid, and quercitrin. Each compound was evaluated for its antioxidant and anti-aging activities in TNF-α-stimulated NHDFs. Among them, ellagic acid exhibited the most potent biological activity and was selected for further mechanistic analysis. Ellagic acid significantly suppressed intracellular reactive oxygen species (ROS) generation and matrix metalloproteinase-1 (MMP-1) secretion (both p < 0.001), while markedly increasing type I procollagen production (p < 0.01). Mechanistic studies demonstrated that ellagic acid inhibited TNF-α-induced phosphorylation of mitogen-activated protein kinases (MAPKs), downregulated cyclooxygenase-2 (COX-2), and upregulated heme oxygenase-1 (HO-1), a key antioxidant enzyme. Additionally, ellagic acid attenuated the mRNA expression of inflammatory cytokines, including interleukin-6 (IL-6) and interleukin-8 (IL-8), indicating its broad modulatory effects on oxidative and inflammatory pathways. Collectively, these findings suggest that ellagic acid is a promising plant-derived bioactive compound with strong antioxidant and anti-inflammatory properties, offering potential as a therapeutic agent for the prevention and treatment of skin aging. Full article
(This article belongs to the Section Bioorganic Chemistry and Medicinal Chemistry)
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24 pages, 1967 KiB  
Article
Water Stress Promotes Secondary Sexual Dimorphism in Ecophysiological Traits of Papaya Seedlings
by Ingrid Trancoso, Guilherme A. R. de Souza, João Vitor Paravidini de Souza, Rosana Maria dos Santos Nani de Miranda, Diesily de Andrade Neves, Miroslava Rakocevic and Eliemar Campostrini
Plants 2025, 14(15), 2445; https://doi.org/10.3390/plants14152445 - 7 Aug 2025
Abstract
Plant genders could express different functional strategies to compensate for different reproductive costs, as females have an additional role in fruit and seed production. Secondary sexual dimorphism (SSD) expression is frequently greater under stress than under optimal growth conditions. The early gender identification [...] Read more.
Plant genders could express different functional strategies to compensate for different reproductive costs, as females have an additional role in fruit and seed production. Secondary sexual dimorphism (SSD) expression is frequently greater under stress than under optimal growth conditions. The early gender identification in papaya may help to reduce orchard costs because the most desirable fruit shape is formed by hermaphrodite plants. We hypothesized that (a) gender ecophysiological phenotyping can be an alternative to make gender segregations in papaya seedlings, and (b) such gender segregation will be more efficient after a short drought exposure than under adequate water conditions. To test such hypotheses, seedlings of two papaya varieties (‘Candy’ and ‘THB’) were exposed to two kind of treatments: (1) water shortage (WS) for 45 h, after which they were well watered, and (2) continuously well-watered (WW). Study assessed the ecophysiological responses, such as stomatal conductance (gs), SPAD index, optical reflectance indices, morphological traits, and biomass accumulation in females (F) and hermaphrodites (H). In WS treatment, the SSD was expressed in 14 of 18 traits investigated, while in WW treatment, the SSD was expressed only in 7 of 18 traits. As tools for SSD expression, gs and simple ratio pigment index (SRPI) must be measured on the first or second day after the imposed WS was interrupted, respectively, while the other parameters must be measured after a period of four days. In some traits, the SSD was expressed in only one variety, or the response of H and F plants were of opposite values for two varieties. The choice of the clearest responses of gender segregation in WS treatment will be greenness index, combination of normalized difference vegetation index (CNDVI), photochemical reflectance index (PRI), water band index (WBI), SRPI, leaf number, leaf dry mass, and leaf mass ratio. If the WW conditions are maintained for papaya seedling production, the recommendation in gender segregation will be the analysis of CNDVI, carotenoid reflectance index 2 (CRI2), WBI, and SRPI. The non-destructive optical leaf indices segregated papaya hermaphrodites from females under both water conditions and eventually could be adjusted for wide-scale platform evaluations, with planned space arrangements of seedlings, and sensor’s set. Full article
(This article belongs to the Section Horticultural Science and Ornamental Plants)
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21 pages, 3488 KiB  
Article
Effects of Continuous Saline Water Irrigation on Soil Salinization Characteristics and Dryland Jujube Tree
by Qiao Zhao, Mingliang Xin, Pengrui Ai and Yingjie Ma
Agronomy 2025, 15(8), 1898; https://doi.org/10.3390/agronomy15081898 - 7 Aug 2025
Abstract
The sustainable utilization of saline water resources represents an effective strategy for alleviating water scarcity in arid regions. However, the mechanisms by which prolonged saline water irrigation influences soil salinization and dryland crop growth are not yet fully understood. This study examined the [...] Read more.
The sustainable utilization of saline water resources represents an effective strategy for alleviating water scarcity in arid regions. However, the mechanisms by which prolonged saline water irrigation influences soil salinization and dryland crop growth are not yet fully understood. This study examined the effects of six irrigation water salinity levels (CK: 0.87 g·L−1, S1: 2 g·L−1, S2: 4 g·L−1, S3: 6 g·L−1, S4: 8 g·L−1, S5: 10 g·L−1) on soil salinization dynamics and jujube growth during a three-year field experiment (2020–2022). The results showed that soil salinity within the 0–1 m profile significantly increased with rising irrigation water salinity and prolonged irrigation duration, with the 0–0.4 m layer accounting for 50.27–74.95% of the total salt accumulation. A distinct unimodal salt distribution was observed in the 0.3–0.6 m soil zone, with the salinity peak shifting downward from 0.4 to 0.5 m over time. Meanwhile, soil pH and sodium adsorption ratio (SAR) increased steadily over the study period. The dominant hydrochemical type shifted from SO42−-Ca2+·Mg2+ to Cl-Na+·Mg2+. Crop performance exhibited a nonlinear response to irrigation salinity levels. Low salinity (2 g·L−1) significantly enhanced plant height, stem diameter, leaf area index (LAI), vitamin C content, and yield, with improvements of up to 12.11%, 3.96%, 16.67%, 16.24%, and 16.52% in the early years. However, prolonged exposure to saline irrigation led to significant declines in both plant growth and water productivity (WP) by 2022. Under high-salinity conditions (S5), yield decreased by 16.75%, while WP declined by more than 30%. To comprehensively evaluate the trade-off between economic effects and soil environment, the entropy weight TOPSIS method was employed to identify S1 as the optimal irrigation treatment for the 2020–2021 period and control (CK) as the optimal treatment for 2022. Through fitting analysis, the optimal irrigation water salinity levels over 3 years were determined to be 2.75 g·L−1, 2.49 g·L−1, and 0.87 g·L−1, respectively. These findings suggest that short-term irrigation of jujube trees with saline water at concentrations ≤ 3 g·L−1 is agronomically feasible. Full article
(This article belongs to the Section Water Use and Irrigation)
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22 pages, 1909 KiB  
Review
Cassava (Manihot esculenta Crantz): Evolution and Perspectives in Genetic Studies
by Vinicius Campos Silva, Gustavo Reis de Brito, Wellington Ferreira do Nascimento, Eduardo Alano Vieira, Felipe Machado Navaes and Marcos Vinícius Bohrer Monteiro Siqueira
Agronomy 2025, 15(8), 1897; https://doi.org/10.3390/agronomy15081897 - 7 Aug 2025
Abstract
Cassava (Manihot esculenta Crantz) is essential for global food security, especially in tropical regions. As an important genetic resource, its genetics plays a key role in crop breeding, enabling the development of more productive and pest- and disease-resistant varieties. Scientometrics, which quantitatively [...] Read more.
Cassava (Manihot esculenta Crantz) is essential for global food security, especially in tropical regions. As an important genetic resource, its genetics plays a key role in crop breeding, enabling the development of more productive and pest- and disease-resistant varieties. Scientometrics, which quantitatively analyzes the production and impact of scientific research, is crucial for understanding trends in cassava genetics. This study aimed to apply bibliometric methods to conduct a scientific mapping analysis based on yearly publication trends, paper classification, author productivity, journal impact factor, keywords occurrences, and omic approaches to investigate the application of genetics to the species from 1960 to 2022. From the quantitative data analyzed, 3246 articles were retrieved from the Web of Science platform, of which 654 met the inclusion criteria. A significant increase in scientific production was observed from 1993, peaking in 2018. The first article focused on genetics was published in 1969. Among the most relevant journals, Euphytica stood out with 36 articles, followed by Genetics and Molecular Research (n = 30) and Frontiers in Plant Science (n = 25). Brazil leads in the number of papers on cassava genetics (n = 143), followed by China (n = 110) and the United States (n = 75). The analysis of major methodologies (n = 185) reveals a diversified panorama during the study period. Morpho-agronomic descriptors persisted from 1978 to 2022; however, microsatellite markers were the most widely used, with 102 records. Genomics was addressed in 87 articles, and transcriptomics in 65. By clarifying the current landscape, this study supports cassava conservation and breeding, assists in public policy formulation, and guides future research in the field. Full article
(This article belongs to the Section Crop Breeding and Genetics)
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19 pages, 1717 KiB  
Article
A Multifaceted Approach to Optimizing Processed Tomato Production: Investigating the Combined Effects of Biostimulants and Reduced Nitrogen Fertilization
by Michela Farneselli, Lara Reale, Beatrice Falcinelli, Muhammad Zubair Akram, Stefano Cimarelli, Eleonore Cinti, Michela Paglialunga, Flavia Carbone, Euro Pannacci and Francesco Tei
Horticulturae 2025, 11(8), 931; https://doi.org/10.3390/horticulturae11080931 - 7 Aug 2025
Abstract
Excessive nitrogen (N) fertilizer usage in agriculture has prompted the exploration of sustainable strategies to enhance nitrogen use efficiency (NUE) while maintaining crop yield and quality. Processed tomatoes (Solanum lycopersicum L.) were grown for two years (2023 and 2024) following a two-way [...] Read more.
Excessive nitrogen (N) fertilizer usage in agriculture has prompted the exploration of sustainable strategies to enhance nitrogen use efficiency (NUE) while maintaining crop yield and quality. Processed tomatoes (Solanum lycopersicum L.) were grown for two years (2023 and 2024) following a two-way factorial randomized complete block (RCBD) design, considering three biostimulants and three N regimes as two factors, to assess their morphophysiological, biochemical, anatomical and yield performances. Nitrogen application significantly influenced biomass accumulation, the leaf area index (LAI), nitrogen uptake and yield with notable comparable values between reduced and optimal nitrogen dose, indicating improved nitrogen use efficiency. Biostimulants showed limited effects alone but enhanced plant performance under reduced nitrogen conditions, particularly improving chlorophyll content, crop growth, N uptake, yield and anatomical adaptations. Moreover, compared to 2024, biostimulant application enhanced tomato growth more evidently in 2023 due to environmental variations, likely due to the occurrence of stress conditions. Importantly, biostimulants, together with N regimes, i.e., optimal and reduced doses, showed improved anatomical traits, especially regarding leaf thickness and thickness between the two epidermises, indicating adaptive responses that may support sustained productivity under N-limited conditions. Among the biostimulants used, the processed tomatoes responded better to protein hydrolysate and endophytic N-fixing bacteria than to seaweed extract. These findings suggest that although biostimulants alone were not affected, integrating them with reduced N fertilization provides a viable strategy for optimizing tomato production, conserving resources and minimizing the environmental impact without compromising yield or quality. Full article
(This article belongs to the Special Issue Effects of Biostimulants on Horticultural Crop Production)
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15 pages, 771 KiB  
Review
Trichoderma: Dual Roles in Biocontrol and Plant Growth Promotion
by Xiaoyan Chen, Yuntong Lu, Xing Liu, Yunying Gu and Fei Li
Microorganisms 2025, 13(8), 1840; https://doi.org/10.3390/microorganisms13081840 - 7 Aug 2025
Abstract
The genus Trichoderma plays a pivotal role in sustainable agriculture through its multifaceted contributions to plant health and productivity. This review explores Trichoderma’s biological functions, including its roles as a biocontrol agent, plant growth promoter, and stress resilience enhancer. By producing various [...] Read more.
The genus Trichoderma plays a pivotal role in sustainable agriculture through its multifaceted contributions to plant health and productivity. This review explores Trichoderma’s biological functions, including its roles as a biocontrol agent, plant growth promoter, and stress resilience enhancer. By producing various enzymes, secondary metabolites, and volatile organic compounds, Trichoderma effectively suppresses plant pathogens, promotes root development, and primes plant immune responses. This review details the evolutionary adaptations of Trichoderma, which has transitioned from saprotrophism to mycoparasitism and established beneficial symbiotic relationships with plants. It also highlights the ecological versatility of Trichoderma in colonizing plant roots and improving soil health, while emphasizing its role in mitigating both biotic and abiotic stressors. With increasing recognition as a biostimulant and biocontrol agent, Trichoderma has become a key player in reducing chemical inputs and advancing eco-friendly farming practices. This review addresses challenges such as strain selection, formulation stability, and regulatory hurdles and concludes by advocating for continued research to optimize Trichoderma’s applications in addressing climate change, enhancing food security, and promoting a sustainable agricultural future. Full article
(This article belongs to the Special Issue Advances in Plant–Soil–Microbe Interactions)
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