Search Results (1,165)

Valued for furniture, crafts, and medicine, Dalbergia odorifera T. C. Chen confronts critically depleted wild populations and slow cultivation growth, necessitating precision nutrient formulation to overcome physiological constraints. Using a ‘3414’ regression design with four levels of N, P, and K, this study identified phosphorus (P) as the most influential nutrient in regulating growth (P > N > K). Maximal growth enhancement occurred under T7 (N2P3K2), with height and basal diameter increments increasing by 239% and 128% versus controls (p < 0.05). Both traits exhibited progressive gains with rising P but unimodal responses to N and K, initially increasing then declining. T7 boosted total biomass by 50% (p < 0.05) with stem-biased partitioning (stem > root > leaf; 52%, 26%, 22%). Photosynthetic capacity increased significantly under T7 (p < 0.05), driven by P-mediated chlorophyll gains (Chla + 70%; Chlb + 75%) and an 82% higher net photosynthetic rate. Metabolic shifts revealed peak soluble sugar in T7 (+139%) and soluble protein in T9 (+226%) (p < 0.05), associated primarily with P and K availability, respectively. Correlation networks revealed significant associations among structural growth, photosynthesis, and metabolism. Principal component analysis established T7 as optimal, defining a “medium-N, high-P medium-K” precision fertilization protocol. These findings elucidate a phosphorus-centered regulatory mechanism governing growth in D. odorifera, providing a scientific foundation for efficient cultivation. Full article

This study aimed to assess the phenotypic and histological characteristics of leaves, stems, and roots of sweet potato (‘Hopungmi’ and ‘Sodammi’, Korean cultivars) under the low-temperature conditions induced by early transplanting. In leaves, early transplanting (ETP) led to reductions in vascular bundle width (from −22.6% to −53.7%), xylem diameter (from −51.6% to −52.6%), palisade parenchyma thickness (from −31.3% to −31.5%), and the palisade parenchyma thickness-to-leaf thickness ratio (from −31.2% to −32.1%), while the total leaf thickness remained unchanged. Principal component 1 (PC1: 69.7%) was positively correlated with vascular characteristics and palisade parenchyma thickness, reflecting enhanced development under optimal transplanting (OTP) and greater photosynthetic capacity. These findings indicate that low temperatures hinder palisade parenchyma development. In stems, ETP reduced stem radius (from −20.3% to −42.1%) and the pith-to-stem radius ratio (from −21.0% to −25.3%) but increased the xylem-to-stem radius ratio (from +45.8% to +47.1%) and the collenchyma-to-stem radius ratio (from +61.5% to +84.7%). PC1 (45.7%) showed positive correlations with xylem and collenchyma ratios and negative correlations with stem radius and pith ratio, suggesting that these anatomical adjustments helped maintain stem rigidity under stress. In roots, ETP significantly reduced root radius (from −78.0% to −94.5%), vascular radius (from −83.9% to −96.9%), cortex thickness (from −68.9% to −80.7%), and the vascular-to-root radius ratio (from −28.6% to −44.7%), while increasing the cortex-to-root radius ratio (from +53.0% to +248.0%). PC1 (93.8%) was positively associated with vascular characteristics and cortex thickness and negatively associated with the cortex-to-root radius ratio. Overall, the low temperatures resulting from early transplanting altered the anatomical structures of leaves, stems, and roots, indicating suboptimal conditions for storage root development. In particular, the vascular bundle radius of sweet potato roots was identified as a crucial indicator for evaluating storage root development, which can be utilized in future breeding strategies. Full article

This study aims to evaluate the feasibility and benefits of integrating photovoltaic (APV) systems with rice cultivation, focusing on growth characteristics, chlorophyll content and fluorescence, yield components, and electricity production. An APV system was installed over a rice paddy area in Namhae-gun, Gyeongsangnam-do, with 607 modules providing a total capacity of approximately 97.12 kW. The Baegokchal variety of rice was cultivated following standard practices, and growth characteristics, chlorophyll content, and fluorescence were measured throughout the growing period. Yield components were analyzed, and electricity production was monitored to assess the performance of the APV system. The rice growing period in 2021 experienced lower than average temperatures and higher rainfall. Despite these conditions, rice in the APV systems showed increased chlorophyll content and fluorescence, indicating an adaptive response to reduced sunlight. Rice plants in APV systems exhibited greater plant height but fewer tillers compared to the control group. Leaves were significantly longer and wider, enhancing photosynthetic efficiency under shading. The yield of rice in APV systems was reduced by approximately 9% compared to the control, less severe than that reported in other studies. The APV system demonstrated stable electricity production, with consistent output throughout the year, despite variations in solar radiation. Integrating photovoltaic systems with rice cultivation is feasible and beneficial, providing a reliable source of renewable energy and enhancing farm income despite a slight reduction in rice yield. This study highlights the potential of APV systems to contribute to sustainable agriculture and renewable energy expansion, suggesting the need for further research on various crops and conditions to optimize system performance. Full article

This study investigates the impact of climate change on key physiological parameters of Greek indigenous grapevine cultivars (Savvatiano, Muscat, Assyrtiko, Mavrodafni, Moschofilero, and Agiorgitiko), using Sauvignon blanc and Merlot as benchmarks. The aim was to identify genotypes with higher photosynthetic dynamics and water use efficiency (WUE) under heat stress and to examine the role of CO₂ enrichment in modulating these responses. Gas exchange measurements showed that short-term exposure to elevated CO₂ (e[CO₂]) (i.e., 700 ppm) enhanced photosynthesis by 37–64%, 77–89%, and 18–68% under control, moderate, and severe heat-stress regimes (23, 35, and 40 °C), respectively. CO₂ enrichment also improved WUE by 61–122%, 96–138%, and 11–63%, with the greatest benefits at 30–33 °C, depending on genotype. Cultivars with strong CO₂-saturated photosynthetic capacity and small stomata, such as Sauvignon blanc and Mavrodafni, showed greater photosynthetic stimulation and WUE improvement from CO₂ elevation. Stomatal traits influenced photosynthesis under ambient CO₂ (a[CO₂]) but not under e[CO₂]. Of the white varieties examined, Sauvignon blanc and Savvatiano showed the best performance under combined e[CO₂] and heat stress, while Assyrtiko and Muscat adapted better to high temperatures at a[CO₂]. Among red cultivars, Mavrodafni showed the highest photosynthetic efficiency at both CO₂ conditions, even under heat stress. The present findings indicate that grapevine varieties exhibit differential responses to elevated temperature and CO₂ levels. A comprehensive understanding of grapevine responses to stress conditions is therefore essential for the selection of cultivars with enhanced adaptation to climate change. Full article

Periphytic algae, as representative aquatic epiphytic communities, play a vital role in the material cycling and energy flow in rivers. Through physiological processes such as photosynthetic carbon fixation and nutrient absorption, they perform essential ecological functions in water self-purification, maintenance of primary productivity, and microhabitat formation. This study investigates the interaction mechanisms between these highly flexible organisms and the hydrodynamic environment, thereby addressing the limitations of traditional hydraulic theories developed for rigid vegetation. By incorporating the coupled effects of biological flexibility and water flow, an innovative nonlinear resistance model with velocity-dependent response is developed. Building upon this model, a coupled governing equation that integrates water flow dynamics, periphytic algae morphology, and layered Reynolds stress is formulated. An analytical solution for the vertical velocity distribution is subsequently derived using analytical methods. Through Particle Image Velocimetry (PIV) measurements conducted under varying flow velocity conditions in an experimental tank, followed by comprehensive error analysis, the accuracy and applicability of the model were verified. The results demonstrate strong agreement between predicted and measured values, with the coefficient of determination R² greater than 0.94, thereby highlighting the model’s predictive capacity in capturing flow velocity distributions influenced by periphytic algae. The findings provide theoretical support for advancing the understanding of ecological hydrodynamics and establish mechanical and theoretical foundations for river water environment management and vegetation restoration. Future research will build upon the established nonlinear resistance model to investigate the dynamic coupling mechanisms between multi-species periphytic algae communities and turbulence-induced pulsations, aiming to enhance the predictive modelling of biotic–hydrodynamic feedback processes in aquatic ecosystems. Full article

Forage sorghum (Sorghum bicolor (L.)) is a cereal native to Africa and belongs to the family Poaceae. It is a forage with a C4 photosynthetic pathway that stands out for its ability to adapt to different environments; it is able to produce even in unfavorable circumstances. The objective of this study was to analyze the attenuating effect of the brassinosteroid hormone in the form of 24-epibrassinolide on forage sorghum plants subjected to water deficit and rehydration. A completely randomized design (CRD) was used in the experiment. A 2 × 3 × 5 factorial arrangement was used, with two water conditions (water deficit and rehydration), three brassinosteroid doses (0 nM, 50 nM, and 100 nM as 24-epibrassinolide), and five replicates. The experiment was conducted in a greenhouse. Sorghum seeds were sown in pots with a capacity of 3 kg of substrate. Analyses were performed on the roots and leaves of sorghum plants at different growth stages. The macronutrients (N, P, K, Ca, and Mg) were analyzed in the soil physics laboratory. As a result, the content of N, P, K, Ca, and Mg decreased under a water deficit and was then restored by the hormone 24-epibrassinolide, which was able to restore these nutrients. The effect of the hormone under rehydration had a positive effect, increasing the levels of nutrients. Given the above, it was possible to conclude that there were no significant divergences between the treatments during the period of irrigation suspension. Among the tested concentrations, 50 nM of 24-epibrassinolide showed the most consistent improvements in nutrient concentrations under water-deficit conditions, suggesting a potential role in mitigating nutritional imbalance during stress. Rehydrated plants maintained nutrient levels similar to the controls regardless of 24-epibrassinolide application. However, it is important to note that nutritional quality indices such as crude protein and total digestible nutrients (TDN) were not evaluated in this study, which limits direct conclusions about the forage nutritional value. Full article

Passiflora edulis propagation relies extensively on grafting, yet the optimization of pruning strategies for scion quality remains empirically guided. This study elucidates the physiological and molecular mechanisms underlying scion quality across five leaf retention treatments (0%, 25%, 50%, 75%, and unpruned control). The 50% partial leaf retention (50% PLR) treatment optimally promoted axillary bud development in passion fruit through coordinated physiological and molecular adaptations. This treatment significantly outperformed other treatments in terms of both bud sprouting rate and growth parameters (including length and diameter). Physiological analyses demonstrated transient auxin accumulation coupled with synchronized antioxidant system activation, maintaining redox homeostasis. Transcriptomic profiling identified upregulation of genes in the auxin signaling pathway and cytokinin activators, while dormancy-related genes were suppressed. These findings establish 50% PLR as an optimal threshold that balances photosynthetic capacity with hormonal regulation, providing a science-based strategy to standardize grafted seedling production, while enhancing scion quality for grafting efficiency. Full article

Walnut (Juglans regia L.), an ecologically and economically important species, requires the elucidation of its salt stress response mechanisms for improved salt tolerance breeding. This study elucidates the physiological and molecular mechanisms through which exogenous methyl jasmonate (MeJA) mitigates salt stress in walnut, providing novel strategies for salt-tolerant cultivar development. This integrated study combined physiological, biochemical, and multi-omics analyses to decipher how exogenous MeJA enhances ROS scavenging through the synergistic activation of phenylalanine (Phe), tryptophan (Trp), and α-linolenic acid pathways, establishing a multilevel antioxidant defense network. MeJA treatment effectively mitigated salt stress-induced oxidative damage, as demonstrated by a significant 16.83% reduction in malondialdehyde (MDA) content, concurrent 11.60%, 10.73% and 22.25% increases in superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) activities, respectively, the elevation of osmoregulatory soluble sugars (SS), and 1.2- to 2.0-fold upregulation of key antioxidant enzyme genes (SOD, POD, APX, GPX, DHAR) and elevated osmoregulatory substances (soluble sugars, SS). Improved photosynthetic parameters (P_n, G_s) and chlorophyll fluorescence efficiency (F_v/F_m) collectively indicated reduced oxidative stress (improved by 7.97–23.71%). Joint metabolomic-transcriptomic analyses revealed MeJA-enhanced ROS scavenging via the coordinated regulation of Phe, Trp, and α-linolenic acid pathways. In summary, MeJA significantly enhanced reactive oxygen species (ROS) scavenging efficiency and comprehensive antioxidant capacity in walnut seedlings through the synergistic regulation of key metabolic pathways, effectively mitigating salt stress. These findings establish a crucial mechanistic foundation for understanding plant salt stress responses and advance the utilization of MeJA-mediated strategies for the genetic improvement of salinity tolerance in walnut. Future research should prioritize optimizing MeJA application protocols and functionally validating key regulatory genes for breeding applications. Full article

The precipitation of minerals, in particular carbonates, is a widespread phenomenon in all ecosystems, where it assumes a high relevance both from a geological and biogeochemical standpoint. Most carbonate rocks are of biological origin and made in an aquatic environment. In particular, bioprecipitation of carbonates is believed to have started in the Mesoproterozoic Era, thanks to a process often driven by photosynthetic microorganisms. Nevertheless, an important contribution to carbonate precipitation is also due to the metabolic activity of heterotrophic bacteria, which is not restricted to specific taxonomic groups or to specific environments, making this process a ubiquitous phenomenon. In this framework, the relationship between carbonatogenic microorganisms and other living organisms assumes a particular interest. This study aims to isolate and identify the culturable heterotrophic bacterial component associated with the coenosarc of Corallium rubrum in order to evaluate the occurrence of strains able to precipitate carbonates. In particular, the study was focused on the identification and characterisation of bacterial strains isolated from a coral coenosarc showing a high carbonatogenic capacity under laboratory conditions. Samples of C. rubrum were taken in the coastal waters of three Italian regions. The concentration of the aerobic heterotrophic microflora colonising C. rubrum coenosarc samples spanned from 3 to 6∙10⁶ CFU/cm². This variation in microbial populations colonising the C. rubrum coenosarc, spanning over 6 orders of magnitude, is not mirrored by a corresponding variability in the colony morphotypes recorded, with the mean being 5.1 (±2.1 sd). Among these bacteria, the carbonatogenic predominant species was Staphylococcus equorum (93% of the isolates), whereas Staphylococcus xylosus and Shewanella sp. accounted only for 3% of isolates each. All these strains showed a remarkable capacity of precipitating calcium carbonate, in the form of calcite crystals organised radially as well crystalised spherulites (S. equorum) or coalescing spherulites (Shewanella sp.). S. xylosus only produced amorphous precipitates of calcium carbonate. All bacterial strains identified were positive both for the production of urease and carbon anhydrase in vitro at 30 °C. It seems that they potentially possess the major biochemical abilities conducive to Ca²⁺ precipitation, as they showed in vitro. In addition, all our carbonatogenic isolates were able to hydrolyse the phytic acid calcium salt and then were potentially able to induce precipitation of calcium phosphates also through such a mechanism. Full article

In arid regions, water scarcity and soil potassium destruction are major constraints on the sustainable development of the jujube industry. In this regard, the use of crop models can compensate for time-consuming and costly field trials to screen for better irrigation regimes, but their predictive accuracy is often compromised by parameter uncertainty. To address this issue, we utilized data from a three-year (2022–2024) field trial (with irrigation at 50%, 75%, and 100% of evapotranspiration and potassium applications of 120, 180, and 240 kg/ha) to simulate the growth process of jujube trees in arid regions using the WOFOST model. In this study, parameter sensitivity analyses were conducted to determine that photosynthetic capacity maximization (A_max), the potassium nutrition index (K_status), the water stress factor (SWF), the water–potassium photosynthetic coefficient of synergy (α), and potassium partitioning weight coefficients (β_i) were the important parameters affecting the simulated growth process of the crop. Path analysis using segmented structural equations also showed that water stress factor (SWF) and potassium nutrition index (K_status) indirectly controlled yield by significantly affecting photosynthesis (path coefficients: 0.72 and 0.75, respectively). The ability of the crop model to simulate the growth process and yield of jujube trees was improved by the introduction of water and potassium parameters (R² = 0.94–0.96, NRMSE = 4.1–12.2%). The subsequent multi-objective optimization of yield and crop water productivity of dates under different combinations of water and potassium treatments under a bi-objective optimization model based on the NSGA-II algorithm showed that the optimal strategy was irrigation at 80% ET_c combined with 300 kg/ha of potassium application. This management model ensures yield and maximizes crop water use efficiency (CWP), thus providing a scientific and efficient irrigation and fertilization regime for jujube trees in arid zones. Full article

The inclusion of the Internet of Things (IoT) in indoor agricultural systems has become a fundamental tool for improving cultivation systems by providing key information for decision-making in pursuit of better performance. This article presents the design and implementation of an IoT-based agricultural system installed in a plant growth chamber for hydroponic cultivation under controlled conditions. The growth chamber is equipped with sensors for air temperature, relative humidity (RH), carbon dioxide (CO₂) and photosynthetically active photon flux, as well as control mechanisms such as humidifiers, full-spectrum Light Emitting Diode (LED) lamps, mini split air conditioner, pumps, a Wi-Fi surveillance camera, remote monitoring via a web application and three Nutrient Film Technique (NFT) hydroponic systems with a capacity of ten plants each. An ATmega2560 microcontroller manages the smart system using the MODBUS RS-485 communication protocol. To validate the proper functionality of the proposed system, a case study was conducted using lettuce crops, in which the impact of different nutrient solution concentrations (50%, 75% and 100%) on the phenotypic development and nutritional content of the plants was evaluated. The results obtained from the cultivation experiment, analyzed through analysis of variance (ANOVA), show that the treatment with 75% nutrient concentration provides an appropriate balance between resource use and nutritional quality, without affecting the chlorophyll content. This system represents a scalable and replicable alternative for protected agriculture. Full article

Peanut cultivation is widely practiced using plastic mulch film, resulting in the accumulation of microplastics/nanoplastics (MPs/NPs) in agricultural soils, potentially negatively affecting peanut growth. To investigate the effects of two polystyrene (PS) sizes (5 μm, 50 nm) and three concentrations (0, 10, and 100 mg L⁻¹) on peanut growth, photosynthetic efficiency, and physiological characteristics, a 15-day hydroponic experiment was conducted using peanut seedlings as the experimental material. The results indicated that PS-MPs/NPs inhibited peanut growth, reduced soil and plant analyzer development (SPAD) values (6.7%), and increased levels of malondialdehyde (MDA, 22.0%), superoxide anion (O₂⁻, 3.8%) superoxide dismutase (SOD, 16.1%) and catalase (CAT, 12.1%) activity, and ascorbic acid (ASA, 12.6%) and glutathione (GSH, 9.1%) contents compared to the control. Moreover, high concentrations (100 mg L⁻¹) of PS-MPs/NPs reduced the peanut shoot fresh weight (16.1%) and SPAD value (7.2%) and increased levels of MDA (17.1%), O₂⁻ (5.6%), SOD (10.6%), POD (27.2%), CAT (7.3%), ASA (12.3%), and GSH (6.8%) compared to low concentrations (10 mg L⁻¹) of PS-MPs/NPs. Notably, under the same concentration, the impact of 50 nm PS-NPs was stronger than that of 5 μm PS-MPs. The peanut shoot fresh weight of PS-NPs was lower than that of PS-MPs by an average of 7.9%. Additionally, we found that with an increasing exposure time of PS-MPs/NPs, the inhibitory effect of low concentrations of PS-MPs/NPs on the fresh weight was decreased by 2.5%/9.9% (5 d) and then increased by 7.7%/2.7% (15 d). Conversely, high concentrations of PS-MPs/NPs consistently reduced the fresh weight. Correlation analysis revealed a clear positive correlation between peanut biomass and both the SPAD values as well as Fv/Fm, and a negative correlation with MDA, SOD, CAT, ASA, and GSH. Furthermore, the presence of PS-MPs/NPs in roots, stems, and leaves was confirmed using a confocal laser scanning microscope. The internalization of PS-MPs/NPs within peanut tissues negatively impacted peanut growth by increasing the MDA and O₂⁻ levels, reducing the SPAD values, and inhibiting the photosynthetic capacity. In conclusion, the study demonstrated that the effects of PS on peanuts were correlated with the PS size, concentration, and exposure time, highlighting the potential risk of 50 nm to 5 μm PS being absorbed by peanuts. Full article

Nano- and microplastic pollution, together with the ongoing rise in global temperatures driven by climate change, represent increasingly critical environmental challenges. Although these stressors often co-occur in the environment, their combined effects on plant systems remain largely unexplored. To test the hypothesis that their interaction may exacerbate the effects observed under each stressor individually, we investigated the response of seedlings of Triticum turgidum to treatments with fluorescent polystyrene nanoplastics under optimal (25 °C) and elevated (35 °C) temperature conditions. We evaluated seedling growth, photosynthetic pigment content, and oxidative stress markers using both biochemical and histochemical techniques. In addition, we assessed enzymatic and non-enzymatic antioxidant responses. The use of fluorescently labeled nanoplastics enabled the visualization of their uptake and translocation within plant tissues. Elevated temperatures negatively affect plant growth, increasing the production of proline, a key protective molecule, and weakly activating secondary defense mechanisms. Nanoplastics disturbed wheat seedling physiology, with these effects being amplified under high temperature conditions. Combined stress enhances nanoplastic uptake in roots, increases oxidative damage, and alters antioxidant responses, reducing defense capacity in leaves while triggering compensatory mechanisms in roots. These findings underscore a concerning interaction between plastic pollution and climate warming in crop plants. Full article

The cessation of traditional mountain grazing has emerged as a principal driver of habitat degradation and the local extinction of Parnassius apollo (Linnaeus, 1758) in Serbia. While previous studies have cited multiple contributing factors, our research provides evidence that the abandonment of extensive livestock grazing has triggered vegetation succession, the disappearance of the larval host plant (Sedum album), and a reduction in microhabitat heterogeneity—conditions essential for the persistence of this stenophagous butterfly species. Through satellite-based analysis of vegetation dynamics (2015–2024), we identified clear structural differences between habitats that currently support populations and those where the species is no longer present. Occupied sites were characterized by low levels of exposed soil, moderate grass coverage, and consistently high shrub and tree density, whereas unoccupied sites exhibited dense encroachment of grasses and woody vegetation, leading to structural instability. Furthermore, MODIS-derived indices (2010–2024) revealed a consistent decline in vegetation productivity (GPP, FPAR, LAI) in succession-affected areas, alongside significant correlations between elevated land surface temperatures (LST), thermal stress (TCI), and reduced photosynthetic capacity. A wildfire event on Mount Stol in 2024 further exacerbated habitat degradation, as confirmed by remote sensing indices (BAI, NBR, NBR2), which documented extensive burn scars and post-fire vegetation loss. Collectively, these findings indicate that the decline of P. apollo is driven not only by ecological succession and climatic stressors, but also by the abandonment of land-use practices that historically maintained suitable habitat conditions. Our results underscore the necessity of restoring traditional grazing regimes and integrating ecological, climatic, and landscape management approaches to prevent further biodiversity loss in montane environments. Full article

Flavonoids serve as crucial plant antioxidants in drought tolerance, yet their antioxidant regulatory mechanisms within mycorrhizal plants remain unclear. In this study, using a two-factor design, trifoliate orange (Poncirus trifoliata (L.) Raf.) seedlings in the four-to-five-leaf stage were either inoculated with Funneliformis mosseae or not, and subjected to well-watered (70–75% of field maximum water-holding capacity) or drought stress (50–55% field maximum water-holding capacity) conditions for 10 weeks. Plant growth performance, photosynthetic physiology, leaf flavonoid content and their antioxidant capacity, reactive oxygen species levels, and activities and gene expression of key flavonoid biosynthesis enzymes were analyzed. Although drought stress significantly reduced root colonization and soil hyphal length, inoculation with F. mosseae consistently enhanced the biomass of leaves, stems, and roots, as well as root surface area and diameter, irrespective of soil moisture. Despite drought suppressing photosynthesis in mycorrhizal plants, F. mosseae substantially improved photosynthetic capacity (measured via gas exchange) and optimized photochemical efficiency (assessed by chlorophyll fluorescence) while reducing non-photochemical quenching (heat dissipation). Inoculation with F. mosseae elevated the total flavonoid content in leaves by 46.67% (well-watered) and 14.04% (drought), accompanied by significantly enhanced activities of key synthases such as phenylalanine ammonia-lyase (PAL), chalcone synthase (CHS), chalcone isomerase (CHI), 4-coumarate:coA ligase (4CL), and cinnamate 4-hydroxylase (C4H), with increases ranging from 16.90 to 117.42% under drought. Quantitative real-time PCR revealed that both mycorrhization and drought upregulated the expression of PtPAL1, PtCHI, and Pt4CL genes, with soil moisture critically modulating mycorrhizal regulatory effects. In vitro assays showed that flavonoid extracts scavenged radicals at rates of 30.07–41.60% in hydroxyl radical (•OH), 71.89–78.06% in superoxide radical anion (O₂^•−), and 49.97–74.75% in 2,2-diphenyl-1-picrylhydrazyl (DPPH). Mycorrhizal symbiosis enhanced the antioxidant capacity of flavonoids, resulting in higher scavenging rates of •OH (19.07%), O₂^•− (5.00%), and DPPH (31.81%) under drought. Inoculated plants displayed reduced hydrogen peroxide (19.77%), O₂^•− (23.90%), and malondialdehyde (17.36%) levels. This study concludes that mycorrhizae promote the level of total flavonoids in trifoliate orange by accelerating the flavonoid biosynthesis pathway, hence reducing oxidative damage under drought. Full article