Search Results (392)

The artichoke (Cynara cardunculus L. subsp. scolymus) is an endemic perennial plant of the Mediterranean area commonly consumed as food. It is known since ancient times for its beneficial properties for human health, among which its antioxidant activity due to polyphenolics stands out. In the frame of our ongoing studies aiming to highlight the biodiversity and the chemodiversity of natural resources, we investigated the phenolic and saponin content of the cultivar “Carciofo di Procida” collected at Procida, an island of the Gulf of Naples (Italy). Along with the edible part of the immature flower, we included in our analyses the stem and the external bracts, generally discarded for food consuming or industrial preparations. The LCMS quali-quantitative profiling of polyphenols (including anthocyanins) and cynarasaponins of this cultivar is reported for the first time. In addition to antioxidant properties, we observed a significant cytotoxic activity due to extracts from external bracts against human neuroblastoma SH-SY5Y cell lines with 43% of cell viability, after 24 h from the treatment (50 μg/mL), and less potent but appreciable effects also against human colorectal adenocarcinoma CaCo-2 cells. This suggests that the different metabolite composition may be responsible for the bioactivity of extracts obtained from specific parts of artichoke and foresees a possible exploitation of the discarded material as a source of beneficial compounds. Full article

Background: The dual nature of cannabis, as both a promising therapeutic tool and a widely used recreational substance with potential risks, raises important societal controversies, including its unclear impacts regarding mental health. This narrative review provides a comprehensive overview of cannabis, addressing (i) its historical context; (ii) its chemical composition and pharmacokinetics; (iii) its pharmacological effects; (iv) its negative impacts on physiological and mental health; (v) its potential use as a drug for the treatment of neurological and psychiatric disorders; (vi) its relationship with the gut microbiome and how this interaction might influence mental functioning; (vii) the pathophysiology, prevalence, comorbidities, and treatment strategies of cannabis use disorder; and (viii) social perspectives on its legalization. Results: Cannabis presents a complex chemical profile and pharmacokinetics that show promise in treating numerous neurological, psychiatric, and psychological conditions. However, its use carries risks, which depend on factors such as compound concentration, dosage, consumption method, frequency of use, and individual vulnerability. Cannabis use disorder seems to be less severe than other substance use disorders, but it still constitutes a significant concern, as its manifestation is not uniform across all users. Conclusions: Cannabis demands a thorough understanding that goes beyond simplistic explanations and prejudices, standing as a plant of substantial clinical significance and highlighting the importance of personalized approaches to its use and increased awareness of how individuals respond to its effects. Full article

Niche complementarity is suggested to be a main driver of productivity overyielding in diverse environments due to enhanced resource use efficiency and reduced competition. Here, we combined multiple different approaches to demonstrate that niche overlap is the most likely cause to explain a lack of overyielding of three tree species when grown in different species combinations. First, in an experimental planting we found no relationship between productivity and species diversity for leaf, wood, or root production (no slope was significantly different from zero), suggesting a lack of niche differences among species. Second, data extracted from the United States Department of Agriculture Forest Inventory and Analysis revealed that the species do not significantly co-occur in natural stands (p = 0.4065) as would be expected if coexistence was common across their entire range. Third, we compared trait differences among our species and found that they are not significantly different in multi-dimensional trait space (p = 0.1724). By combining multiple analytical approaches, we provide evidence of potential niche overlap that precludes coexistence and a positive diversity–productivity relationship between these three tree species. Full article

The transition zone between the Qinghai–Tibet and Loess Plateaus is an important ecological functional area and carbon (C) reservoir in China. Studying the main drivers of C density changes in forest ecosystems is crucial to enhance the C sink potential of those ecosystems in ecologically fragile regions. In this study, four stand types at different succession stages in the transition zone of Xinglong Mountain were selected as the study objective. The C densities of the ecosystem, vegetation, plant debris, and soil of each stand type were estimated, and the related driving factors were quantified. The results showed that the forest ecosystem C density continuously increased significantly with natural succession (381.23 Mg/hm² to 466.88 Mg/hm²), indicating that the ecosystem has a high potential for C sequestration with progressive forest succession. The increase in ecosystem C density was mainly contributed to by the vegetation C density, which was jointly affected by the vegetation characteristics (C sink, mean diameter at breast height, mean tree height), litter C/N (nitrogen), and surface soil C/N, with factors explaining 95.1% of the variation in vegetation C density, while the net effect of vegetation characteristics was the strongest (13.9%). Overall, this study provides a new insight for understanding the C cycle mechanism in ecologically fragile areas and further improves the theoretical framework for understanding the C sink function of forest ecosystems. Full article

Cancers, particularly those resistant to treatment, stand as one of the most significant challenges in medicine. Frequently, available therapies need to be improved, underscoring the necessity for innovative treatment modalities. Over the years, there has been a resurgence of interest in natural plant substances, which have been traditionally overlooked as anticancer agents. A prime example of this is natural antioxidants, such as acteoside (ACT) and orientin (ORI), which offer novel approaches to cancer treatment, emphasizing liver cancer compared to other cancer types. They reduce oxidative stress by activating the Nrf2/ARE pathway and exhibit anticancer activity, e.g., decreasing Bcl-2 and Bcl-XL expression and increasing Bax levels. This review explores the individual effects of ACT and ORI and their synergistic interactions with sorafenib, temozolomide, 5-fluorouracil (for ACT), celecoxib, and curcumin (for ORI), highlighting their enhanced anticancer efficacy. In addition, ACT and ORI successfully integrate into various drug delivery systems (DDSs), including metal-containing carriers such as nanoparticles (NPs), nanoshells (NSs), quantum dots (QDs), and liposomes as representative examples of lipid-based drug delivery systems (LBDDSs). Advanced methods, including nanotechnology, offer potential solutions to low bioavailability, paving the way for the use of these substances in anticancer therapy. Full article

The two-spotted spider mite Tetranychus urticae Koch (Acari: Tetranychidae) is a major pest of horticultural, ornamental, fruit, and strawberry crops worldwide. Currently, various management tools have been explored for this pest, with nanoparticles being one of them, which stand out for their characteristics and multiple effects. This study evaluated the effects of green-synthesized gold nanoparticles (AuNPs) on the mortality and repellency of T. urticae and its natural predator Phytoseiulus persimilis under laboratory conditions, as well as their efficacy in greenhouse tomatoes against T. urticae. In the laboratory, a biological window for AuNPs (50–100 mg L⁻¹) on the pest and predator was established using a residual film method and a free-choice assay. In the greenhouse, four concentrations (300, 500, 750, and 1000 mg L⁻¹) were evaluated via foliar application at 10-day intervals. The results showed susceptibility to AuNPs in all stages of T. urticae and the adult P. persimilis. The death times from AuNPs were similar in both species. Furthermore, the AuNPs were selective for the pest rather than the natural enemy. In greenhouses, AuNPs affected T. urticae populations in tomato plants, and significant differences were observed on some continuous and final agronomic variables (associated with fruits). This study showed that T. urticae and P. persimilis were susceptible to green-synthesized AuNPs. AuNPs can be a management tool, although studies on other non-target species and estimating agronomic effects on other crops are recommended. Full article

Common buckthorn (Rhamnus cathartica L.) is a small tree that forms dense stands, displacing native plant species and threatening natural forest habitats in its introduced range in North America. Removal via cutting is labor intensive and often ineffective due to vigorous resprouting. Although chemical control methods are effective, they can negatively affect sensitive ecosystems. A mycoherbicide that selectively kills buckthorn would provide an additional method for control. In the present study, fungi were collected from dying buckthorn species (Frangula alnus Mill., Rhamnus cathartica, Ventia alnifolia L’Hér) located at 19 sites across Minnesota and Wisconsin for their potential use as mycoherbicides for common buckthorn. A total of 412 fungi were isolated from samples of diseased tissue and identified via DNA extraction and sequencing. These fungi were identified as 120 unique taxa belonging to 81 genera. Of these fungi, 46 species belonging to 26 genera were considered to be canker or root-rot pathogens of woody plants, including species in Cytospora, Diaporthe, Diplodia, Dothiorella, Eutypella, Fusarium, Hymenochaete, Irpex, Phaeoacemonium, and others. A future study testing the pathogenicity of these putative pathogens of buckthorn is now needed to assess their utility as potential mycoherbicide agents for control of common buckthorn. Full article

In this work, we evaluated the silvicultural and ecological parameters of Populus bolleana Lauche trees growing in conditions of anthropogenic pollution, using the example of one of the largest megacities of the Donetsk ridge, the city of Donetsk. The objectives of this study included determining the level of anthropogenic load of the territory; conducting dendrological studies to assess morphometric and allometric parameters, age structure, and condition of P. bolleana stands under the influence of environmental factors; as well as completing biomechanical studies to assess and predict the mechanical stability of stands. A total of 1109 plants growing in areas with increased anthropogenic load and in the control areas were studied. The model territories of the study were located in the city of Donetsk on Fallen Communards Avenue (length of field routes: 2.6 km) and Ilyicha Avenue (length of field routes: 9.7 km). Control plantings grew on the territory of the Donetsk botanical garden and residential (dormitory) districts of the city. The age structure of P. bolleana plantations remained uniform throughout the city for 50–55 years due to the fact that the landscaping was under a single state program. In the steppe zone in the south of the East European Plain, with a high level of anthropogenic load and severe natural climatic factors, the critical age of P. bolleana (55 years) was determined. The condition of plantations and their morphometric indices correlate with the level of anthropogenic load of the city (H, Dbase, DBH). Under control conditions, the plants are in good condition with signs of weakening (2 points). Under conditions of increased anthropogenic load, the plants are in a severely weakened condition (3 points). A total of 25% of the plants in the sample are in critical condition (4–5 points). The main damages to the crowns and trunks of plants include core rot, mechanical damage to bark and tissues, the development of core rot through the affected skeletal branch, crown thinning, and drying. P. bolleana trees are valued for their crown area and ability to retain dust particles from the air. The analysis of experimentally obtained data on the crown area showed that in the initial phases of ontogenesis, the average deviation in the crown area of plants does not depend on the place of growth. Due to artificial narrowing and sanitary pruning of the crown, as well as skeletal branches dying along the busiest highways, the values do not exceed 22–23 m² on average, with an allometric coefficient of 0.35–0.37. When comparing this coefficient in the control areas, the crown area in areas with a high level of anthropogenic load is 36 ± 11% lower. For trees growing under the conditions of the anthropogenic load of an industrial city and having reached the critical age, mechanical resistance varied depending on the study area and load level. At sites with a high level of pollution of the territory, a significant decrease in indicators was revealed in comparison with the control (mcr—71%, EI—75%, RRB—43%). Having analyzed all the obtained data, we can conclude that, until the age of 50–55 years, P. bolleana retains good viability, mechanical resistance, and general allometric ratios, upon which the stability of the whole plant depends. Even with modern approaches and tendencies toward landscaping with exotic introductions, it is necessary to keep P. bolleana as the main species in dendrobanocenoses. Full article

Monoculture plantation systems face increasing challenges in sustaining ecosystem multifunctionality (EMF) under intensive management and climate change, with long-term functional trajectories remaining poorly understood. Although biodiversity–EMF relationships are well-documented in natural forests, the drivers of multifunctionality in managed plantations, particularly age-dependent dynamics, require further investigation. This study examines how stand development influences EMF in Castanopsis hystrix L. plantations, a dominant subtropical timber species in China, by assessing six ecosystem functions (carbon stocks, wood production, nutrient cycling, decomposition, symbiosis, and water regulation) of six forest ages (6, 10, 15, 25, 30, and 34 years). The results demonstrate substantial age-dependent functional enhancement, with carbon stocks and wood production increasing by 467% and 2016% in mature stand (34 year) relative to younger stand (6 year). Nutrient cycling and water regulation showed intermediate gains (6% and 23%). Structural equation modeling identified plant diversity and microbial community composition as direct primary drivers. Tree biomass profiles emerged as the strongest biological predictors of EMF (p < 0.01), exceeding abiotic factors. These findings highlight that C. hystrix plantations can achieve high multifunctionality through stand maturation facilitated by synergistic interactions between plants and microbes. Conservation of understory vegetation and soil biodiversity represents a critical strategy for sustaining EMF, providing a science-based framework for climate-resilient plantation management in subtropical regions. Full article

Coral restoration is essential for recovering depleted populations and reef ecological functions. However, its effect on enhancing fish assemblages remains understudied. This study investigated the integration of 3D-printed and natural Staghorn coral (Acropora cervicornis) out-planting to assess their role in enhancing benthic spatial complexity and attracting fish communities. Conducted between 2021 and 2023 at Culebra Island, Puerto Rico, we employed a before-after-control-impact (BACI) design to test four treatments: natural A. cervicornis, 3D-printed corals, mixed stands of 3D-printed and natural corals, and non-restored controls. Fish assemblages were monitored through stationary counts. Results showed that integrating 3D-printed and natural corals enhanced fish assemblages and their ecological functions. Significant temporal changes in fish community structure and biodiversity metrics were observed, influenced by treatment and location. Herbivore abundance and biomass increased over time, especially in live coral and 3D-printed plots. Reefs with higher rugosity exhibited greater Scarid abundance and biomass post-restoration. Piscivore abundance also rose significantly over time, notably at Tampico site. Fishery-targeted species density and biomass increased, particularly in areas with live and 3D-printed coral out-plants. Fish assemblages became more complex and diverse post-restoration, especially at Tampico, which supported greater habitat complexity. Before restoration, fish assemblages showed a disturbed status, with biomass k-dominance curves above abundance curves. Post-out-planting, this trend reversed. Control sites showed no significant changes. The study demonstrates that restoring fast-growing branching corals, alongside 3D-printed structures, leads to rapid increases in abundance and biomass of key fishery species, suggesting its potential role promoting faster ecosystem recovery and enhanced coral demographic performance. Full article

Dysphania ambrosioides, commonly known as epazote, is a medicinal plant of great relevance in traditional Latin American medicine. Its cultural roots and pharmacological properties have made it an object of study for phytochemical research. An artificial intelligence (AI) tool was utilized to assist in reviewing scientific information regarding D. ambrosioides. An initial search was conducted in the Scopus database using the keywords epazote, D. ambrosioides, anti-helminthic, antioxidant, and antimicrobial, which yielded a total of 814 publications. To select the most relevant articles, this AI tool based on natural language processing (available online and free of charge) was applied, which analyzed the keywords that appeared in the titles and abstracts of the works and clustered them, leading to a reduction of 86.73% in the number of studies. D. ambrosioides stands out for its rich composition of bioactive compounds, which give the plant a wide range of therapeutic properties, including antiparasitic activity, through which it is effective against several parasites, such as helminths and protozoa, due to its schistosomicidal, nematocidal and antimalarial action. Additionally, it has shown antimicrobial, antioxidant, and anticancer properties as it contains compounds that help fight cell damage caused by free radicals. Epazote represents a rich source of compounds with a wide therapeutic range. However, much research is required to understand the mechanisms of action of these compounds and to evaluate their safety and efficacy in clinical trials. Full article

The long-standing need for a modern public transportation system in Lebanon, a developing country of the Middle East with an almost exclusive dependence on costly and polluting passenger cars, has become more pressing in recent years due to the worsening economic crisis and the onset of hyperinflation. This study investigates the potential reductions in energy use, emissions, and costs from the possible introduction of natural gas, hybrid, and battery-electric buses compared to traditional diesel buses in local real driving conditions. Four operating conditions were considered including severe congestion, peak, off-peak, and bus rapid transit (BRT) operation. Battery-electric buses are found to be the best performers in any traffic operation, conditional on having clean energy supply at the power plant and significant subsidy of bus purchase cost. Natural gas buses do not provide significant greenhouse gas emission savings compared to diesel buses but offer substantial reductions in the emission of all major pollutants harmful to human health. Results also show that accounting for additional energy consumption from the use of climate-control auxiliaries in hot and cold weather can significantly impact the performance of all bus technologies by up to 44.7% for electric buses on average. Performance of all considered bus technologies improves considerably in free-flowing traffic conditions, making BRT operation the most beneficial. A vehicle mix of diesel, natural gas, and hybrid bus technologies is found most feasible for the case of Lebanon and similar developing countries lacking necessary infrastructure for a near-term transition to battery-electric technology. Full article

The aim of this research was to record changes in the population structure of epigeic spider assemblages in the Central European Danube Delta (Slovakia) as a result of habitat management measures and the impact of human intervention. During this research (2020–2023), we assessed the impact of management measures on newly planted forest stands and the effect of grazing in semi-natural conditions, and carried out diversity monitoring in flooded meadows. A total of 6344 individuals belonging to 89 spider species were collected by pitfall traps and identified. Using spatial modelling, we observed the following: (i) there are differences between the structures of managed and unmanaged forest stands (larger number of taxa); (ii) the differences in the number of individuals between study plots and years were statistically significant; (iii) the trend analysis of spider communities showed that study plots that underwent management intervention are expected to see an increase in the number of individuals in the future; and (iv) in the areas that did not undergo management, the number of species was stable. Using spiders as bioindicators could therefore answer the question of whether anthropogenic disturbance disrupts ecological stability. This approach utilizes spiders to assess the sustainability of the landscape. Full article

As the largest terrestrial carbon pool, forest ecosystems play a pivotal role in climate change mitigation through greenhouse gas regulation. This study estimated the carbon sequestration potential of arbor forests at the county-level scale in Mentougou District, Beijing, based on subcompartment vector data from forest surveys and employed the Intergovernmental Panel on Climate Change (IPCC) carbon stock–biomass difference methodology. Additionally, using 2020 as the baseline year, the research projected carbon sink potential and carbon sequestration–oxygen release values for 2030 and 2060 by applying the carbon stock change methodology and the carbon sequestration–oxygen release value methodology. The results showed that there is a total carbon stock of 2.198 million tonnes (Mt) C in Mentougou, with an average storage density of 33.4 t C/ha. Natural broadleaf forests constituted the dominant carbon pool (79.2%), followed by planted coniferous stands (11.9%), collectively accounting for 91.1% of the regional arboreal carbon storage. In the future, the district’s arboreal carbon stock is projected to reach 3.17 Mt C in 2030 and 4.82 Mt C in 2060, with cumulative sequestration reaching 0.97 Mt C and 2.63 Mt C, respectively. It is evident that the carbon storage dynamics in Mentougou were governed by three principal determinants: (1) natural broadleaf forests dominate carbon storage (1.559 Mt C) in Mentougou, exceeding planted coniferous stands by 6.7-fold; (2) carbon storage decreases progressively with younger age classes, while carbon density increases steadily with stand maturity; (3) mid-elevation slopes (600–1200 m) concentrate 48% of regional stocks, with shaded slopes being optimal carbon sinks, and slope position gradients reveal topography-driven carbon accumulation patterns, confirming scale-dependent material transport effects. The value of carbon fixation and oxygen release of existing arbor forests in Mentougou District was CNY 6.12 billion, and this is predicted to reach CNY 8.84 billion by 2030, with a further anticipated increase to CNY 13.45 billion by 2060. Our analysis provides empirical evidence and quantitative support for forestry carbon sink initiatives at the regional scale and thus promotes the achievement of dual-carbon goals proposed by the Chinese government. Full article

Boreal forests have historically been regarded as some of the largest terrestrial carbon (C) sinks. However, increased soil organic matter (SOM) decomposition due to forest harvesting and post-harvest silviculture (e.g., site preparation, planting, and managing for competing vegetation) may exacerbate the effects of climate warming and shift boreal forests from being C sinks to C sources. We used an established stand-scale, fully replicated, experimental study to identify how two levels of forest management (harvesting = Harvest Only, and harvesting with post-harvest silviculture = Harvest Plus) influence SOC dynamics at three boreal forest sites varying in soil texture. Each site was surveyed for forest floor (litter and F/H horizons) and mineral soils pre-harvest (0) and 5, 14, and 20 years post-harvest. We predicted that sites harvested and left to revegetate naturally would have the lowest SOC stocks after 20 years, as sites that were planted and managed for competing vegetation would recover faster and contribute to a larger nutrient pool, and that the sand-dominated site would have the largest SOC losses following harvest due to the inherently lower ability of sand soils to chemically and/or physically protect SOC from decomposition following harvest. Over a 20-year period, both forest management treatments generally resulted in reduced total (litter, F/H, and mineral horizon) SOC stocks compared with the control: the Harvest Only treatment reduced overall SOC stocks by 15% at the silt-dominated site and 31% at the clay-dominated site but increased overall SOC stocks by 4% at the sand-dominated site, whereas the Harvest Plus treatment reduced overall SOC stocks by 32% at the sand- and silt-dominated sites and 5% at the clay-dominated site. This suggests that harvesting and leaving plots to revegetate naturally on sand-dominated sites and harvesting followed by post-harvest silviculture on clay-dominated sites may minimize total SOC losses at similar sites, though a full replicated field experiment is needed to test this hypothesis. Most treatment effects in this study were observed only in the second decade post-harvest (14 and 20 years post-harvest), highlighting the importance of long-term field experiments on the effects of forest harvesting and post-harvest silviculture. This research improves our understanding of the relationship between C dynamics, forest management, and soil texture, which is integral for developing sustainable management strategies that optimize C sequestration and contribute to the resilience of boreal forest ecosystems in the face of climate change. Full article