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27 pages, 1493 KB  
Article
Effect of Organic Soil Amendments and Vineyard Topographic Position on the Chemical Composition of Syrah, Trincadeira, Alicante Bouschet, and Antão Vaz Grapes (Vitis vinifera L.) in the Alentejo Wine Region
by Matteo Pierini, Shrika G. Harjivan, Nicolò Sieli, Maria João Cabrita, Sérgio Prats, Sofia Catarino and Jorge M. Ricardo-da-Silva
Environments 2026, 13(1), 44; https://doi.org/10.3390/environments13010044 - 9 Jan 2026
Viewed by 196
Abstract
Climate change and unsustainable agricultural practices are triggering land degradation in semi-arid Mediterranean regions. Organic amendments, such as mulching materials, have shown promising potential to mitigate these impacts by improving soil chemical, physical, and biological properties, while enhancing grapevine growth and productivity. This [...] Read more.
Climate change and unsustainable agricultural practices are triggering land degradation in semi-arid Mediterranean regions. Organic amendments, such as mulching materials, have shown promising potential to mitigate these impacts by improving soil chemical, physical, and biological properties, while enhancing grapevine growth and productivity. This study evaluated the effects of wheat straw mulch (M) and wheat straw combined with biochar (MB), together with vineyard topography (bottom vs. top), on grape chemical and phenolic composition in four Vitis vinifera L. cultivars (Syrah, Trincadeira, Alicante Bouschet, and Antão Vaz) grown in the Alentejo wine region. Grapes were sampled separately at top and bottom topographic positions, and classical and phenolic parameters were analyzed. The application of M and MB significantly modified must composition, mainly through changes in nitrogen and sugar levels across topographic positions. Only MB exhibited stronger effects, enhancing must quality, while MB and M reduced bottom–top variability. Similar patterns and positional effects were observed for phenolic and color parameters. Both organic treatments lowered total monomeric anthocyanin concentrations, although positional differences with wheat straw mulch were found. The results highlight that combining soil management with topography and variety response can optimize grape phenolic composition and promote sustainable viticulture through targeted, site-specific mulching strategies. Full article
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9 pages, 1166 KB  
Proceeding Paper
Yield Stability of Selected Potato Cultivars Under Mulch and Fungicide Applications Across Different Environments
by Nosipho Precious Minenhle Phungula, Sandile Thamsanqa Hadebe, Lucky Sithole, Morgan Nadioo and Nomali Ziphorah Ngobese
Biol. Life Sci. Forum 2025, 54(1), 6; https://doi.org/10.3390/blsf2025054006 - 31 Dec 2025
Viewed by 93
Abstract
Smallholder farmers’ yields fluctuate yearly due to the variability of climate, resources, and diseases. The study aimed to assess genotypes-by-environment interactions under different management practices using additive main effects and multiplicative interaction models. Potato cultivars (Mondial, Electra, Sababa, and Panamera) were grown in [...] Read more.
Smallholder farmers’ yields fluctuate yearly due to the variability of climate, resources, and diseases. The study aimed to assess genotypes-by-environment interactions under different management practices using additive main effects and multiplicative interaction models. Potato cultivars (Mondial, Electra, Sababa, and Panamera) were grown in five environments (Mbalenhle, Hlathikhulu, Mbhava, Stezi, and Gobizembe) for three seasons (2021–2023). Potatoes were planted under mulch (non-mulched and mulched) and fungicide (sprayed and unsprayed) management practices. The results revealed that the genotype–environment effect had a minimal contribution to tuber yield, ranging from 8.42% to 11.01% across management practices. For instance, in the absence of fungicide application with mulch and non-mulched practices, resulted in genotype effects of 69.92% and 60.62% and environments effects of 20.52% and 30.95%, respectively. Full article
(This article belongs to the Proceedings of The 3rd International Online Conference on Agriculture)
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16 pages, 3428 KB  
Article
Effects of Biochar Addition on Topsoil Carbon–Nitrogen Cycling and CO2 Emissions in Reduced-Nitrogen, Film-Mulched Drip-Irrigated Silage Maize Systems
by Zhonghao Jiang, Yanhua Lu, Heng Zhang, Guang Li, Yan Tan, Yingying Zhu, Yang Xie and Bingsheng Wang
Agronomy 2026, 16(1), 10; https://doi.org/10.3390/agronomy16010010 - 19 Dec 2025
Viewed by 344
Abstract
This study conducted a systematic evaluation over two years (2023–2024) through field experiments to assess the regulatory effects of biochar on soil properties, carbon and nitrogen cycling, and CO2 emissions under mulched drip irrigation with varying nitrogen application levels. The core findings [...] Read more.
This study conducted a systematic evaluation over two years (2023–2024) through field experiments to assess the regulatory effects of biochar on soil properties, carbon and nitrogen cycling, and CO2 emissions under mulched drip irrigation with varying nitrogen application levels. The core findings indicate that the effects of biochar are strongly dependent on the nitrogen levels. Under reduced nitrogen conditions, biochar demonstrated a synergistic benefit: with a 15% nitrogen reduction (N2-BC), it significantly enhanced soil water retention (increasing moisture by 68.6% at the tasseling stage); with a 30% nitrogen reduction (N1-BC), it improved soil structure (bulk density decreased by 2.1%, porosity increased by 4.3%). Additionally, biochar differentially activates soil carbon and nitrogen pools: under the 30% nitrogen reduction treatment (N1-BC), soil organic carbon increased to 8.34 g kg−1 during the jointing stage, while dissolved organic carbon reached 0.536 g kg−1 at tasseling, and total nitrogen content rose significantly. Notably, the regulatory effect of biochar on CO2 emissions shifted toward marked suppression as nitrogen input decreased (N1-BC), achieving a net cumulative reduction of 21.4% under deep nitrogen reduction treatment. Correlation analysis further integrated these processes, demonstrating that improvements in the soil physical structure are closely linked to enhanced carbon and nitrogen cycling. This study clarifies that in reduced-N systems, the application of biochar can synergistically achieve “carbon sequestration–nitrogen conservation–emission reduction,” providing a basis for developing green, low-C farmland production models. Full article
(This article belongs to the Section Soil and Plant Nutrition)
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27 pages, 5995 KB  
Article
Optimizing Water and Nitrogen Management Strategies to Unlock the Production Potential for Onion in the Hexi Corridor of China: Insights from Economic Analysis
by Xiaofan Pan, Haoliang Deng, Guang Li, Qinli Wang, Rang Xiao, Wenbo He and Wei Pan
Plants 2026, 15(1), 6; https://doi.org/10.3390/plants15010006 - 19 Dec 2025
Viewed by 394
Abstract
Water and nitrogen are the key factors restricting the productivity improvement of onion in the Hexi Oasis. Unreasonable water and fertilizer management not only increases input costs, but also causes environmental pollution of farmland soil, thereby affecting the sustainable development of agriculture. To [...] Read more.
Water and nitrogen are the key factors restricting the productivity improvement of onion in the Hexi Oasis. Unreasonable water and fertilizer management not only increases input costs, but also causes environmental pollution of farmland soil, thereby affecting the sustainable development of agriculture. To explore the effects of the water–nitrogen interaction and optimized combination schemes on onion yield, water–nitrogen use efficiency, and economic benefits under mulched drip irrigation in the Hexi Oasis, a four-year (2020–2023) water–nitrogen coupling regulation experiment was conducted at the Yimin Irrigation Experimental Station in Minle County, Hexi Corridor. The onion was used as the test crop and three irrigation levels were established, based on reference crop evapotranspiration (ETc): low water (W1, 70% ETc), medium water (W2, 85% ETc), and sufficient water (W3, 100% ETc), as well as high nitrogen N3 (330 kg·ha−1), medium nitrogen N2 (264 kg·ha−1), and low nitrogen N1 (198 kg·ha−1). Meanwhile, no nitrogen application N0 (0 kg·ha−1) was set as the control at three irrigation levels. This study analyzed the effects of different water and nitrogen supply conditions on onion quality, yield, water–nitrogen use efficiency, and economic benefits. A water–nitrogen economic benefit coupling model was established to optimize water–nitrogen combination schemes targeting different economic objectives. The results revealed that medium-to-high water–nitrogen combinations were beneficial for improving onion quality, while excessive irrigation and nitrogen application inhibited bulb quality accumulation. Both yield and economic benefits increased with the increasing amount of irrigation, whereas excessive nitrogen application showed a diminishing yield-increasing effect, simultaneously increasing farm input costs and ultimately reducing the economic benefits. In the four-year experiment, the N3W3 treatment in 2020 achieved the highest yield, economic benefits, and net profit, reaching 136.93 t·ha−1, 20,376.3 USD·ha−1, and 14,320.8 USD·ha−1, respectively, with no significant difference from the N2W3 treatment. From 2021 to 2023, the N2W3 treatment achieved the highest yield, economic benefits, and net profit, averaging 130.87 t·ha−1, 28,449.5 USD·ha−1, and 21,881.5 USD·ha−1, respectively. Lower irrigation and nitrogen application rates mutually restricted the water and nitrogen utilization, resulting in low water use efficiency, irrigation water use efficiency, nitrogen partial factor productivity, and nitrogen agronomic use efficiency. The relationship between the irrigation amount, nitrogen application rate, and the economic benefits of onion fits a bivariate quadratic regression model. This model predicts that onion’s economic benefits are highly correlated with the actual economic benefits, with analysis revealing a parabolic trend in economic benefits as water and nitrogen inputs increase. By optimizing the model, it was determined that when the irrigation amount reached 100%, the ETc and nitrogen application rate was 264 kg·ha−1, and the economic benefits were close to the target range of 27,000–29,000 USD·ha−1; this can be used as the optimal water and nitrogen management model and technical reference for onion in the Hexi Oasis irrigation area, which can not only ensure high yield and quality but also improve the use efficiency of water and nitrogen. Full article
(This article belongs to the Special Issue Water and Nitrogen Management in the Soil–Crop System (3rd Edition))
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25 pages, 6042 KB  
Article
Ridge-Furrow Planting with Nitrogen Application Enhanced Rainfed Maize Yield and Water Productivity by Improving Leaf Photosynthetic Capacity
by Zhenlin Lai, Hao Kong, Mahmood Hemat, Zhenqi Liao, Shengzhao Pei, Han Wang, Zhijun Li and Junliang Fan
Agronomy 2025, 15(12), 2878; https://doi.org/10.3390/agronomy15122878 - 15 Dec 2025
Viewed by 316
Abstract
Leaf photosynthesis plays an important role in maize growth and yield components due to its involvement in dry matter partitioning and organ formation. Nevertheless, how varying planting patterns affect maize leaf photosynthesis, chlorophyll fluorescence and subsequently maize yield remains poorly understood, particularly at [...] Read more.
Leaf photosynthesis plays an important role in maize growth and yield components due to its involvement in dry matter partitioning and organ formation. Nevertheless, how varying planting patterns affect maize leaf photosynthesis, chlorophyll fluorescence and subsequently maize yield remains poorly understood, particularly at various nitrogen rates. A two-season field experiment was performed on rainfed maize in 2021 and 2022 to explore the responses of photosynthetic physiological characteristics, leaf N and chlorophyll contents, chlorophyll fluorescence parameters, grain yield and water productivity to various planting patterns and N rates. The experiment included six planting patterns, i.e., flat planting without mulching (CK), flat planting with straw mulching (SM), ridge mulched with transparent film and furrow without mulching (RP1), flat planting with full transparent film mulching (FM1), ridge mulched with black film and furrow without mulching (RP2), and flat planting with full black film mulching (FM2). Additionally, there were two nitrogen rates, i.e., 0 kg N ha−1 (N0) and 180 kg N ha−1. The results showed that nitrogen application significantly improved leaf physiological characteristics. Under various planting patterns, leaf photosynthetic pigments, leaf area duration, leaf nitrogen content, QYmax and ΦPSII ranked as RP2 > RP1(FM2) > FM1 > SM(CK) in 2021, and RP2(RP1) > FM1(FM2) > SM(CK) in 2022. No significant variations were observed in water productivity (WP) among different film colors, with overall performance of RP2(FM2) > RP1(FM1) > SM > CK. WP significantly improved by 36.14% and 25.15% under N1 compared to N0 in 2021 and 2022, respectively. This pattern paralleled the fluctuation in water consumption intensity. Compared to CK, RP significantly increased leaf nitrogen content (29.3%), total Chl content (16.0%), QYmax (6.39%), ΦPSII (32.01%), and net photosynthesis rate (14.2%), thereby significantly improving grain yield (46.35%) and WP (27.69%), while reducing evapotranspiration (6.84%). Yield performance ranked as RP2 > (RP1 and FM2) > FM1 > SM > CK in 2021 and RP2 > RP1 > (FM1 and FM2) > SM > CK in 2022. Overall, RP2N1 obtained the highest principal component scores in both years, suggesting great potential to improve leaf photosynthetic physiological characteristics, thereby increasing grain production and ensuring food security in rainfed maize cultivation areas. Full article
(This article belongs to the Collection Crop Physiology and Stress)
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23 pages, 3382 KB  
Article
Optimizing Ridge–Furrow Configuration and Nitrogen Rate to Enhance Wheat Nitrogen Use Efficiency Under Diverse Climate and Soil Conditions
by Ting Pan, Zeyu Liu, Liuyang Yan, Fu Chen, Juanling Wang, Xuefang Huang and Yueyue Xu
Agriculture 2025, 15(24), 2543; https://doi.org/10.3390/agriculture15242543 - 8 Dec 2025
Viewed by 372
Abstract
Optimizing field cropping practices to improve nitrogen use efficiency is imperative to promote intensive and sustainable wheat production. As a cultivation method commonly adopted in arid and semi-arid regions globally, the ridge–furrow mulching system (RFMS) is capable of efficiently harvesting rainfall, reduce evaporation [...] Read more.
Optimizing field cropping practices to improve nitrogen use efficiency is imperative to promote intensive and sustainable wheat production. As a cultivation method commonly adopted in arid and semi-arid regions globally, the ridge–furrow mulching system (RFMS) is capable of efficiently harvesting rainfall, reduce evaporation losses, enhancing soil moisture levels in the root zone, and boosting crop productivity. However, the combined effects of varying ridge–furrow ratios (RD), ridge heights (RH), and nitrogen application rates (RN) on nitrogen fertilizer bias productivity (PFPN) under the influence of climatic conditions, soil types, and field management practices remain poorly understood due to a lack of systematic evaluation. This study conducted a meta-analysis of 462 comparative datasets from 98 research projects to reveal the interactive effects of RFMS and nitrogen fertilizer across climatic gradients. The results showed that RH, RD, and RN increased by 23.78%, 22.37%, and 23.07% respectively (p < 0.05), with the most significant enhancement of PFPN being demonstrated by RH. The most significant improvement in PFPN was observed when RD = 1:1, R < 10 cm, and RN > 200 kg∙hm−2, with PFPN increasing by 27.7%, 29.50%, and 29.32% respectively (p < 0.05). Climatic and soil physico-chemical factors and field management practices are the key factors influencing the RFMS. When average annual evapotranspiration (AE) < 1000, RN > 200 has the best effect on nitrogen utilization efficiency, while under the condition of AE > 1500, RN < 100 is more effective. In terms of mulching strategy, full mulching of ridges and furrows is recommended in areas with severe drought and low temperatures, while mulching only ridges or furrows is more appropriate in areas with relatively mild climate. The present study provides a scientific basis for the optimal design of ridge–furrow mulching configuration and nitrogen application level. This is achieved by considering climatic conditions, soil fertility, and field management in agro-ecosystems in arid and semi-arid areas. Full article
(This article belongs to the Section Agricultural Soils)
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21 pages, 3165 KB  
Article
Response of Nitrogen Cycling in Alfalfa (Medicago sativa L.) Grassland Systems to Cropping Patterns and Nitrogen Application Rates: A Quantitative Analysis Based on Nitrogen Balance
by Yaya Duan, Jianxin Yin, Yuanbo Jiang, Haiyan Li, Wenjing Chang, Yanbiao Wang, Minhua Yin, Yanxia Kang, Yanlin Ma, Yayu Wang and Guangping Qi
Plants 2025, 14(23), 3647; https://doi.org/10.3390/plants14233647 - 29 Nov 2025
Viewed by 422
Abstract
An imbalance between the supply and demand of nutrients within the crop–soil system has resulted from the prevalent practice of excessive fertilization in agricultural agriculture. In order to increase crop growth, improve resource usage efficiency, and reduce agricultural nonpoint source pollution, appropriate cropping [...] Read more.
An imbalance between the supply and demand of nutrients within the crop–soil system has resulted from the prevalent practice of excessive fertilization in agricultural agriculture. In order to increase crop growth, improve resource usage efficiency, and reduce agricultural nonpoint source pollution, appropriate cropping management techniques are essential. This study examined the effects of four nitrogen application rates (0 kg·ha−1 (C0), 80 kg·ha−1 (C1), 160 kg·ha−1 (C2), and 240 kg·ha−1 (C3)) and three alfalfa cropping systems (traditional flat planting, FP; ridge-covered biodegradable mulch, JM; and ridge-covered conventional mulch, PM) on soil inorganic nitrogen transport, nitrogen allocation within alfalfa plants, and soil N2O emissions. Throughout the alfalfa growth phase, the dynamics of nitrogen balance within the soil–plant–atmosphere system were quantitatively examined. The findings showed: (1) The concentrations of soil NO3–N and NH4+–N rose with the rate of nitrogen application but decreased with soil depth. The PMC3 treatment had the largest inorganic nitrogen reserves at the end of the alfalfa growth period. (2) The pattern of PM > JM > FP for nitrogen uptake and nitrogen accumulation in biomass in alfalfa leaves and stems peaked at the C2 nitrogen treatment rate. (3) As nitrogen application rates increased, grass-land N2O emission flow and total emissions also followed PM > JM > FP. (4) The PMC2 treatment showed apparent nitrogen balances of 9.73 kg·ha−1 and 1.84 kg·ha−1 during the two-year growing season, with apparent nitrogen loss rates of 6.08% and 1.15%, respectively, both significantly lower than other treatments, according to nitrogen balance analysis. In summary, the nitrogen application pattern combining ridge-covering conventional plastic mulch with moderate nitrogen application levels can achieve nitrogen balance in alfalfa grassland systems within the Yellow River irrigation district of Gansu Province, China, and similar ecological zones. Full article
(This article belongs to the Special Issue Water and Nutrient Management for Sustainable Crop Production)
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18 pages, 4266 KB  
Article
Biodegradable Film Mulching Increases Soil Respiration: A Two-Year Field Comparison with Polyethylene Film Mulching in a Semi-Arid Region of Northern China
by Xiaowei Liu, Dejun Wang, Mahepali Bazhabaike, Mingdong Zhou and Tao Yin
Agronomy 2025, 15(11), 2631; https://doi.org/10.3390/agronomy15112631 - 16 Nov 2025
Viewed by 743
Abstract
Biodegradable film mulching is increasingly used to replace polyethylene in agriculture, but effects on soil respiration (SR) and components remain unclear, especially during degradation. This study investigated biodegradable mulching’s regulation of SR, root-derived respiration (RDR), and non-root-derived respiration (NRDR) under varying phases. A [...] Read more.
Biodegradable film mulching is increasingly used to replace polyethylene in agriculture, but effects on soil respiration (SR) and components remain unclear, especially during degradation. This study investigated biodegradable mulching’s regulation of SR, root-derived respiration (RDR), and non-root-derived respiration (NRDR) under varying phases. A two-year field experiment was conducted in a rainfed maize system in northern China, comparing conventional tillage with biodegradable film mulching (BM), conventional tillage with polyethylene film mulching (PM), and conventional tillage without mulching (CT). Continuous measurements of soil CO2 concentration (SCC), temperature, water content, and respiration components were used to assess dynamic responses. Results showed that BM enhanced SR and shifted peak timing, with the SR peaking at 106 days after sowing (DAS) under BM, 91.8 DAS under PM, and 91.2 DAS under CT, mainly through a more sustained RDR (BM peak at 103 DAS with a broader peak and greater cumulative RDR than PM and CT). As the biodegradable plastic film degraded, NRDR was higher during the degradation phase, consistent with a priming-like response. These phase-dependent effects suggest that BM first facilitates root growth then serves as a microbial substrate. Moreover, elevated SCC was positively associated with both RDR and NRDR, indicating that CO2 may function as a regulatory signal rather than a passive byproduct of respiration. These findings reveal distinct temporal mechanisms by which BM influences soil carbon fluxes and offer mechanistic insights into the sustainable application of biodegradable film mulching. Future research should evaluate long-term effects on microbial community composition, soil carbon balance, and potential trade-offs with crop productivity and environmental risks. Full article
(This article belongs to the Special Issue Microplastics in Farmland and Their Impact on Soil)
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21 pages, 2308 KB  
Article
Incorporation of Natural Biostimulants in Biodegradable Mulch Films for Agricultural Applications: Ecotoxicological Evaluation
by Chelo Escrig Rondán, Celia Sevilla Gil, Pablo Sanz Fernández, Juan Francisco Ferrer Crespo and Cristina Furió Sanz
Polymers 2025, 17(22), 3027; https://doi.org/10.3390/polym17223027 - 14 Nov 2025
Viewed by 790
Abstract
This study deals with the incorporation of biostimulants of natural origin in a biodegradable polymeric matrix, with the aim of developing mulch films that, when degraded in the soil, release bioactive compounds that improve soil quality and favor the agronomic growth of crops. [...] Read more.
This study deals with the incorporation of biostimulants of natural origin in a biodegradable polymeric matrix, with the aim of developing mulch films that, when degraded in the soil, release bioactive compounds that improve soil quality and favor the agronomic growth of crops. Three types of commercial biostimulants were used: one based on seaweed extract, one on lignosulfonates, and one on plant-derived essential amino acids. To ensure the thermal stability of the biostimulant compounds during processing, thermogravimetric analyses (TGAs) were carried out, and a methodology based on the adsorption of the biostimulants onto porous substrates was developed, enabling their effective incorporation into the polymeric matrix. The formulations obtained have been processed by blown film extrusion at a pilot scale. In addition, the presence of film residues in soil was analyzed by pyrolysis–gas chromatography–mass spectrometry (Py-GC/MS). The results indicate that the proposed methodology supports the integrity of the biostimulants in the films obtained. After the incubation period studied, complete degradation of the biopolymer and the absence of film residues in the soil were confirmed. Furthermore, it was confirmed that this final product had no adverse effects on organisms that were representative of the two end-of-life scenarios, with the exception of the film functionalized with the commercial biostimulant based on seaweed extract, which showed a negative effect on terrestrial higher plants. Full article
(This article belongs to the Special Issue New Progress in Biodegradable Polymeric Materials)
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14 pages, 4077 KB  
Article
Effects of Rice Straw Size on Flow Velocity and Rill Erosion: A Laboratory-Scale Experiment
by Misagh Parhizkar, Manuel Esteban Lucas-Borja and Demetrio Antonio Zema
Environments 2025, 12(11), 421; https://doi.org/10.3390/environments12110421 - 7 Nov 2025
Viewed by 538
Abstract
The residues of rice production could be used as a mulch to reduce the effects of rill erosion on long and steep hillslopes. However, there is a need to identify the most effective size of this residue to apply as a countermeasure of [...] Read more.
The residues of rice production could be used as a mulch to reduce the effects of rill erosion on long and steep hillslopes. However, there is a need to identify the most effective size of this residue to apply as a countermeasure of rill erosion, exploring its effect on hydraulic variables. Several investigations have focused on the anti-erosive effects of other crop residues, while experiences on rice straw applications to reduce rill erosion are still lacking. To fill this gap, this study has measured the variability in flow velocity, stream power and the resulting soil loss in a rill covered by rice straw. Flume experiments simulating rill erosion have been carried out comparing soil loss among treatments with rice straw (dose of 3 tonnes ha−1 and lengths between 20 and 70, 80 and 130, or 140 and 190 mm) and a non-mulched control. Moreover, a multiple regression model that predicts soil loss for a rill cover with rice straw of a given length has been proposed. The application of rice straw reduced the soil loss by at least 20% compared to bare soils. The most suitable size of the applied straw was 90 to 130 mm, which reduces soil loss by 45%. Finer straw (20 to 70 mm) did not significantly improve the soil’s resistance to rill erosion. The beneficial effects of straw must be ascribed to the reduction in flow velocity due to the presence of straw, as shown by accurate power equations regressing the soil loss to this variable. In spite of some limitations (small experimental scale, local environmental conditions, and low incorporation level of the substrate), the results are useful for land managers and hydrologists for soil conservation in hillslopes subjected to intense rill erosion and with similar climatic and hydrological and geomorphological conditions as the case study. Full article
(This article belongs to the Special Issue New Insights in Soil Quality and Management, 2nd Edition)
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12 pages, 580 KB  
Article
Development of a PCR Assay for the Detection of Legionella micdadei in the Environment
by William N. Bélanger, Martine Bastien, Eve Bérubé, Martin Gagnon, Yesmine G. Sahnoun, Valérie Dancause, Karel Boissinot, Cindy Lalancette, Christian Riel-Roberge, Marieve Jacob-Wagner, Sylvie Trottier, Damien Biot-Pelletier, Annie Ruest, Isabelle Tétreault, Mathieu Thériault and Sandra Isabel
Infect. Dis. Rep. 2025, 17(5), 131; https://doi.org/10.3390/idr17050131 - 17 Oct 2025
Viewed by 554
Abstract
Background/Objectives: Legionella micdadei is a clinically significant species within the Legionella genus, requiring accurate detection methods, surveillance, and precise clinical diagnosis. Our objective was to develop a sensitive polymerase chain reaction (PCR) assay specific for L. micdadei to detect its presence in environmental [...] Read more.
Background/Objectives: Legionella micdadei is a clinically significant species within the Legionella genus, requiring accurate detection methods, surveillance, and precise clinical diagnosis. Our objective was to develop a sensitive polymerase chain reaction (PCR) assay specific for L. micdadei to detect its presence in environmental specimens. Methods: We targeted the 23S–5S intergenic spacer region, which can differentiate Legionella spp. We tested the detection of L. micdadei with 20 strains and determined the limit of detection with 2 strains. We verified assay specificity with 17 strains of other Legionella spp., 62 strains of other bacterial and fungal genera, and three human DNA specimens. We evaluated intra- and inter-run precision. We tested 15 environmental specimens (water, swabs of water faucets, mulch, and soil) by PCR. Results: The PCR assay demonstrated 100% analytical specificity (no cross-reactivity with non-targeted species), 100% inclusivity (detection of all L. micdadei strains), and high precision, with a coefficient of variation ≤ 2% across replicates. The limit of detection was estimated at 5 genomic DNA copies per reaction. We detected L. micdadei in environmental specimens. Conclusions: This PCR assay enables accurate detection of L. micdadei and is not subject to competition with other Legionella spp., thereby addressing limitations of current broad-spectrum Legionella approaches. The evaluation supports its application in environmental detection for surveillance. Full article
(This article belongs to the Special Issue Prevention, Diagnosis and Treatment of Infectious Diseases)
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18 pages, 960 KB  
Article
Quality Risk Identification and Fuzzy Comprehensive Assessment of Land Trusteeship Services in China
by Yunlong Sui and Lianghong Yu
Land 2025, 14(10), 2027; https://doi.org/10.3390/land14102027 - 10 Oct 2025
Cited by 1 | Viewed by 522
Abstract
The quality risks of land trusteeship services are increasingly prominent, leading to reduced crop yields for farmers and land degradation; however, relevant research remains insufficient. This paper aims to identify and evaluate the quality risk level of land trusteeship services. It comprehensively adopts [...] Read more.
The quality risks of land trusteeship services are increasingly prominent, leading to reduced crop yields for farmers and land degradation; however, relevant research remains insufficient. This paper aims to identify and evaluate the quality risk level of land trusteeship services. It comprehensively adopts a field survey, web crawler technology, and expert consultation methods to identify quality risk types, and then uses the fuzzy comprehensive evaluation method to assess the risk level based on survey data from Chinese farmers. The main conclusions are as follows: (1) Overall, the quality risk level of land trusteeship services is at a relatively high risk level. In terms of spatio-temporal patterns, the quality risk level shows an upward trend, and the quality risk level of mid-production services is increasing at the fastest rate. There are significant variations in service quality risk across prefecture-level cities in the Shandong Province of China. (2) In terms of risk heterogeneity, the quality risk level of small-scale pure farmers is higher than that of part-time farmers and large professional farmers, in that order. The quality risk level of the “farmer + service organization” model is higher than that of the “farmer + intermediary + service organization” model. According to the order of the quality risk level of different crops, the ranking (from highest to lowest) is cash crops, wheat, and corn. (3) The high quality risks of land trusteeship services will impact the multifunctionality of land systems. It exacerbates the land pollution and fertility degradation because of excessive application of chemical inputs like pesticides, fertilizers, and mulch by service organizations. It consequently destroys ecological systems, hinders sustainable agricultural development, and impacts farmers’ income and national food security by reducing yields. The research findings contribute to controlling the quality risks of land trusteeship services and protecting land. Full article
(This article belongs to the Section Land Systems and Global Change)
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17 pages, 1752 KB  
Article
Methodological Study on Maize Water Stress Diagnosis Based on UAV Multispectral Data and Multi-Model Comparison
by Jiaxin Zhu, Sien Li, Wenyong Wu, Pinyuan Zhao, Xiang Ao and Haochong Chen
Agronomy 2025, 15(10), 2318; https://doi.org/10.3390/agronomy15102318 - 30 Sep 2025
Viewed by 704
Abstract
In response to water scarcity and low agricultural water-use efficiency in arid regions in Northwest China, this study conducted field experiments in Wuwei, Gansu Province, from 2023 to 2024. It aimed to develop a water stress diagnosis model for spring maize to provide [...] Read more.
In response to water scarcity and low agricultural water-use efficiency in arid regions in Northwest China, this study conducted field experiments in Wuwei, Gansu Province, from 2023 to 2024. It aimed to develop a water stress diagnosis model for spring maize to provide a scientific basis for precision irrigation and water management. In this work, two irrigation methods—plastic film-mulched drip irrigation (FD, where drip lines are laid on the soil surface and covered with film) and plastic film-mulched shallow-buried drip irrigation (MD, where drip lines are buried 3–7 cm below the surface under film)—were tested under five irrigation gradients. Multispectral UAV remote sensing data were collected from key growth stages (i.e., the jointing stage, the tasseling stage, and the grain filling stage). Then, vegetation indices were extracted, and the leaf water content (LWC) was retrieved. LWC inversion models were established using Partial Least Squares Regression (PLSR), Random Forest (RF), and Support Vector Regression (SVR). Different irrigation treatments significantly affected LWC in spring maize, with higher LWC under sufficient water supply. In the correlation analysis, plant height (hc) showed the strongest correlation with LWC under both MD and FD treatments, with R2 values of −0.87 and −0.82, respectively. Among the models tested, the RF model under the MD treatment achieved the highest prediction accuracy (training set: R2 = 0.98, RMSE = 0.01; test set: R2 = 0.88, RMSE = 0.02), which can be attributed to its ability to capture complex nonlinear relationships and reduce multicollinearity. This study can provide theoretical support and practical pathways for precision irrigation and integrated water–fertilizer regulation in smart agriculture, boasting significant potential for broader application of such models. Full article
(This article belongs to the Section Water Use and Irrigation)
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43 pages, 1450 KB  
Review
Bio-Based and Nanostructured Polymers for Sustainable Protection of Cultural Heritage and Medicinal Crops: Convergence of Heritage Science, Circular Bioeconomy, and Environmental Protection
by Irina Fierascu, Anda Maria Baroi, Roxana Ioana Matei, Toma Fistos, Irina Elena Chican, Cristina Emanuela Enascuta, Sorin Marius Avramescu and Radu Claudiu Fierascu
Polymers 2025, 17(19), 2582; https://doi.org/10.3390/polym17192582 - 24 Sep 2025
Viewed by 1351
Abstract
Polymers have long been central to modern materials science, but their durability has also made them major contributors to environmental pollution. A new generation of bio-based and nanostructured polymers is now reshaping this field, offering materials that are functional, reversible, and sustainable. This [...] Read more.
Polymers have long been central to modern materials science, but their durability has also made them major contributors to environmental pollution. A new generation of bio-based and nanostructured polymers is now reshaping this field, offering materials that are functional, reversible, and sustainable. This review examines their role across three interconnected domains: cultural heritage conservation, the protection of medicinal and aromatic plants (MAPs), and environmental sustainability. In heritage science, polymers are moving away from synthetic resins toward renewable systems such as chitosan, nanocellulose, and PLA, which provide stability while remaining reversible and compatible with delicate substrates. In agriculture, biodegradable coatings, controlled-release carriers, and edible films are improving MAP protection, extending shelf life, and reducing reliance on synthetic pesticides. In environmental applications, polymers are being reinvented as solutions rather than problems—through degradable mulches, functional hydrogels, and nanocomposites that clean soils and waters within a circular economy framework. Looking across these domains reveals strong synergies. The same principles—biodegradability, multifunctionality, and responsiveness—apply in each context, turning polymers from passive barriers into intelligent, adaptive systems. Their future success will depend not only on chemistry but also on life-cycle design, policy alignment, and public trust, making polymers key enablers of sustainability. Full article
(This article belongs to the Section Circular and Green Sustainable Polymer Science)
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31 pages, 4501 KB  
Review
Shifting from Tillage to Cover Cropping in Warm Climate Viticulture: Seeking the Optimal Balance
by Harsh Tiwari, Ginevra Canavera, Francesco Pelusi and Stefano Poni
Agronomy 2025, 15(10), 2245; https://doi.org/10.3390/agronomy15102245 - 23 Sep 2025
Viewed by 1735
Abstract
Vineyard sustainability increasingly focuses on transitioning from traditional soil management practices, such as tillage and herbicides, to environmentally friendly methods like cover cropping and mulching. While this strategy works in cool climates with abundant rainfall, its application in warmer areas is not advisable [...] Read more.
Vineyard sustainability increasingly focuses on transitioning from traditional soil management practices, such as tillage and herbicides, to environmentally friendly methods like cover cropping and mulching. While this strategy works in cool climates with abundant rainfall, its application in warmer areas is not advisable due to potential disadvantages, such as water and nutrient competition from cover crops, which may outweigh the benefits. We examine the pros and cons of vineyard tillage, including data on evaporation rates from wet and dry tilled soils. We explore methodologies to quantify competition between vine roots and grass roots, focusing on distinguishing native versus spontaneous vegetation, duration and extent of cover cropping, species used in sown mixtures, and cover crop water use rates. Novel soil management practices are discussed as alternatives to traditional green manuring, such as mid-row rolling and sub-row sward mulching. The review updates recent approaches for establishing native or sown under-vine cover crops, which, with irrigation, might control native weeds while colonizing shallow soil, allowing grapevine roots to penetrate deeper, moistened soil layers. Promising grasses include creeping species such as Glechoma hederacea, Trifolium subterraneum, and Hieracium pilosella. Finally, we describe three soil management protocols: two suited to dry farm conditions and one involving blue water availability, which may mitigate cover crop competition for water and nutrients while maintaining benefits such as reduced soil erosion, increased soil organic matter, carbon sequestration, and improved machinery access. Full article
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