Search Results (208)

Biogenic nanoparticles have recently emerged as promising bacterial growth inhibitors, requiring low concentrations and not producing harmful byproducts. However, knowledge gaps remain regarding how different extraction techniques affect nanoparticle synthesis, thereby influencing their replicability and scalability across various applications. To address these knowledge gaps, this study compared six extracts derived from Moringa oleifera biomass for the synthesis of iron oxide nanoparticles. Multivariate statistical analyses correlated extraction methods with biomolecule content (polyphenols, flavonoids, carbohydrates, proteins), iron percentage, and E. coli growth inhibition. All extracts showed varying concentrations of biomolecules, and different extraction methods were preferable for specific components. Flavonoids were best extracted by salting-out, while infusion methods were better for obtaining carbohydrates. Higher percentages of iron (22.77%) were linked to the presence of polyphenols and flavonoids. Nanoparticles prepared using salting-out and infusion extraction from leaf biomass displayed the highest efficiency in inhibiting E. coli growth, up to a dilution factor of 4. The outcomes of this research study provide an in-depth understanding of the role of specific biomolecules in biogenic nanoparticle synthesis, confirming that both synthesis yield and application effectiveness depend on the extract preparation method. Full article

Moringa oleifera (MO) is a versatile non-traditional feed supplement rich in bioactive compounds. The objective of this study was to examine the effects of MO leaf (MOL) polysaccharide (MOLP) intake as a natural product on broiler chicken production and antioxidant indices. A polysaccharide with a molecular weight of 182.989 kDa was isolated from MOL in a previous study. Broiler chickens were allocated at random into four groups receiving varying doses of MOLP (0, 0.1, 0.2 and 0.4 g/kg feed) for three weeks. Feed intake (FI), average daily feed ingestion (ADFI), feed conversion ratio (FCR), and body weight gain (BWG) were monitored. Serological markers, including total protein (TP), albumin (ALB), globulin (GLO), albumin-to-globulin ratio (ALB/GLO), creatinine (CREA), as well as the activities of total superoxide dismutase (T-SOD), glutathione peroxidase (GSH-Px), total antioxidant capacity (T-AOC) and the concentrations of malondialdehyde (MDA) were assessed. Results from days 21 to 28 demonstrated that the high dose of MOLP significantly enhanced BWG, ADFI, liver and bursa indices compared to the control group. Additionally, TP and GLO, T-SOD, GSH-Px, T-AOC and MDA levels were elevated (p < 0.05). In conclusion, MOLP supplementation, particularly at 0.4 g/kg feed, positively impacted broiler chicken growth performance and antioxidant indices, suggesting its potential as a valuable feed additive. Full article

Celtis africana, a rare plant native to southwestern Saudi Arabia, was explored for the first time as a source for the green synthesis of silver nanoparticles (AgNPs). Catechol-bearing phenolic amides in the aqueous leaf extract acted as both reducing and capping agents, enabling eco-friendly AgNP fabrication. The synthesized AgNPs were characterized using SEM, TEM, XRD, UV-Vis, and FTIR, revealing predominantly spherical nanoparticles with an average size of 9.28 ± 0.11 nm, a face-centered cubic crystalline structure, and a pronounced surface plasmon resonance at 424 nm. HPLC analysis confirmed the presence of caffeoyltryamine in the extract, while UV-Vis and FTIR indicated its attachment to the AgNP surface. The AgNPs exhibited broad-spectrum antimicrobial activity against Gram-positive bacteria (S. aureus, MRSA and E. faecalis) and Gram-negative bacteria (E. coli, K. pneumoniae, S. typhimurium, and P. aeruginosa), as well as pathogenic fungi such as C. albicans, C. glabrata, C. parapsilosis, and C. krusei with performance comparable to or exceeding that of AgNPs from Artemisia vulgaris, Moringa oleifera, and Nigella sativa. The MIC and MBC values for S. aureus, MRSA, E. coli, and S. typhimurium were consistently 6.25 µg/mL and 25 µg/mL, respectively, reflecting strong inhibitory and bactericidal effects at low concentrations. MTT assays demonstrated selective cytotoxicity, showing higher viability in normal human skin fibroblasts (HSF) than in MCF-7 breast cancer cells. The AgNPs also displayed strong antioxidant activity (IC₅₀ = 5.41 µg/mL, DPPH assay) and efficient catalytic reduction of 4-nitrophenol (4-NP) and methylene blue (MB), with rate constants of 0.0165 s⁻¹ and 0.0047 s⁻¹, respectively, exceeding most reported values. These findings identify Celtis africana as a promising source for eco-friendly AgNPs with strong antimicrobial, antioxidant, and catalytic properties for broad biological and environmental applications. Full article

Moringa oleifera leaves are a protein-rich source containing all essential amino acids and offering high nutritional value. Ultrasound-assisted extraction (UAE) has emerged as an efficient method to improve protein recovery while enhancing the structural and functional properties of plant proteins. This study aimed to improve protein extraction from M. oleifera leaves using UAE and to characterize the nutritional composition and gel-related properties of the resulting protein concentrate. Chosen conditions were a solubilization pH of 11.68, 20 min of ultrasound treatment, and precipitation at pH 4.5, resulting in an extraction yield of 79.90% and protein content of 53.97%. Moringa oleifera leaf flour (MOF) contained 29.38% protein, 37.98% dietary fiber, and high mineral levels (1751.85 mg/100 g of calcium; 512.55 mg/100 g of magnesium). Compared with MOF, the M. oleifera protein concentrate (MOPC) showed a 21.4% increase in essential amino acids, with leucine and lysine being the most abundant. Functionally, MOPC exhibited 24.26% solubility at pH 2, complete gelation at pH 8, 58.66% emulsifying capacity with 79.52% stability at pH 10, and 21.11% foaming capacity with 94.44% stability at pH 2. The gel-forming ability was the most promising characteristic, highlighting the potential of MOPC as a natural structuring agent in gel-based food systems and functional formulations. Full article

Despite the extensive cultivation of Moringa trees in tropical regions, understanding of accession-specific performance across diverse agroecological zones remains inadequate. Thus, this study evaluated the growth, adaptability, and leaf yield performance of 12 Moringa accessions (11 M. oleifera and 1 M. stenopetala) over three years in a subtropical climate (Pretoria, South Africa). Seeds were planted in seedling trays in the glasshouse at the University of Pretoria’s experimental farm. Vigorous seedlings were transplanted to the field at the Roodeplaat experimental site of the Agricultural Research Council two months after establishment, following a randomized complete block design (RCBD). Data were measured on establishment (emergence, survival), growth and yield parameters, and monitored plant health via leaf greenness, vigour, chlorosis, and pest and disease incidence. Accessions exhibited substantial variation for most traits, except for stem diameter. Moringa stenopetala showed the highest initial emergence rate but later displayed lower survival rates than most M. oleifera accessions. Survival rates, morphological features (plant height, canopy diameter, and branching), visual scores for leaf greenness and plant vigour, and leaf yield (fresh and dry) varied considerably among the accessions. Moringa oleifera A2 consistently performed well, exhibiting vigorous growth, the maximum survival rate (78%), and fresh leaf production (6206 kg ha⁻¹). Accessions A3 and A8 showed intermediate yield and longevity, indicating potential for cultivation or breeding. Conversely, M. oleifera A10 and M. stenopetala markedly underperformed in most traits, limiting their cultivation potential. Based on multi-year performance, A2 is suggested for large-scale cultivation due to its vigour, yield, and stress tolerance, while A3 and A8 hold breeding potential. The study emphasizes the critical role of genetic variation and selection in enhancing Moringa productivity under subtropical environments. Future work should focus on genetic characterization and agronomic practices optimization of superior accessions. Full article

This study assessed the combined application of poultry manure (Pm), biochar (B), and coenzyme A (CoA) into soils to enhance Moringa oleifera Lam. growth, biomass yield, and nutritional and phytochemical composition. This approach allowed us to cover the gap of knowledge on sustainable, low-cost agronomic management alternatives suitable for smallholder systems. To achieve this objective a field experiment was conducted using three treatments (control (no amendment), Pm + B, and Pm + B + CoA) and four consecutive harvests were monitored. Morphological traits (height, stem diameter, number of branches, and leaf yield) were recorded, and phytochemical analyses of glucosinolates and (poly)phenols were performed via HPLC-DAD-ESI/MSn. Mineral and trace elements were quantified by ICP-OES. The main results retrieved allowed describing the capacity of the combined use of Pm + B + CoA to enhance plant growth and productivity, thus increasing the moringa trees’ height of 226.3 by 39.5%, on average, relative to control plants. ILeaf yield and branch number augmented up to 7.0-fold and 2.5-fold, respectively, under amendment treatments. Petiole girth also increased significantly by >50% (p < 0.01). Phytochemically, Pm + B + CoA significantly elevated total phenolics, vicenin-2, and quercetin acetyl-hexoside in leaves by 2.8-fold, on average, relative to control. The glucosinolate content also augmented as a result of the soil amendments assayed by 51.0%, on average, in stems and petioles, under Pm + B + CoA, compared to control samples. From these results, it can be concluded that the combined use of poultry manure, biochar, and CoA significantly improved M. oleifera growth, biomass yield, and nutritional quality, with a particular efficiency concerning (poly)phenolic accumulation. This low-cost, sustainable amendment strategy provides a viable agronomic solution in regions suffering socioeconomic constraints that hinder access to high-cost agronomic management options. Therefore, this approach effectively links ecological soil management with improved productivity, nutritional value, and potential for food industries. Full article

Moringa oleifera, renowned for its medicinal potency, was investigated to discern the impact of varying storage temperatures (4 °C, 25 °C, 40 °C) and light conditions (dark and light) on the quality attributes of its leaf powder during a 12-month storage period. The study encompassed comprehensive analyses of phytochemical levels, nutritional properties, microbial contamination, and colour changes in response to these diverse storage environments. The lightness L* colour value changed significantly (40 to 60) from baseline tests when stored at 40 °C in transparent packaging. Results highlighted distinct variations in phytochemical composition and nutritional content based on the interplay between temperature and light conditions. Lower temperatures, particularly 4 °C, in both dark and light environments, demonstrated superior preservation of bioactive compounds, with mean values for quercetin-3-rutinoside of 3.34 µg/g and 3.19 µg/g, respectively; both are significantly higher compared to other treatments (p < 0.05). This trend was also observed for rutin, chlorogenic acid, and quercetin. Conversely, higher temperatures (25 °C, 40 °C) coupled with light exposure hastened degradation, notably impacting phytochemical stability. Microbial proliferation was evident in elevated temperatures, indicating potential safety risks. Further observations unveiled significant colour changes within the leaf powder, notably influenced by storage temperatures and light exposure. Lower temperatures exhibited diminished colour alterations compared to higher temperatures, underscoring their impact on product quality. This study underscores the critical role of controlled storage conditions, especially cooler temperatures and reduced light exposure, in maintaining the potency and quality of M. oleifera leaf powder. Recommendations advocate for stringent temperature control (preferably 4 °C) and light shielding during storage to uphold phytochemical stability and mitigate microbial proliferation. While this study provides valuable insights into temperature-mediated alterations, future research avenues should delve deeper into elucidating the underlying mechanisms of colour changes and long-term temperature effects on phytochemical and nutritional integrity. Full article

Objective: The study investigates the dietary effects of Moringa oleifera leaf powder on obesity, blood biochemical parameters, and organ health in hyperlipidemic zebrafish (Danio rerio). Methodology: Adult hyperlipidemic zebrafish (n = 56/group) were fed for 12 weeks either with a high-cholesterol diet (HCD, 4% w/w) or HCD supplemented with 0.5% (w/w) M. oleifera leaf powder (0.5% MO) or HCD with 1.0% (w/w) M. oleifera leaf powder (1.0% MO). At different time points (0 to 12 weeks), the survivability and body weight (BW) of zebrafish were measured, while various biochemical and histological evaluations were performed after 12 weeks of feeding the respective diets. Additionally, an in silico approach was used to assess the binding interactions of MO phytoconstituents with 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase. Results: Following 12-week supplementation, higher zebrafish survivability was observed in the MO-supplemented groups compared to the survivability of the HCD group. Relative to the initial BW, only 4% BW enhancement was observed post 12 weeks of dietary intake of 1.0% MO, in contrast to 27% BW gain in the HCD group. MO supplementation at both (0.5% and 1.0%) effectively mitigates the HCD-induced dyslipidemia and significantly minimizes the atherogenic coefficient and atherogenic index. Similarly, MO reduces elevated blood glucose levels, the ALT/AST ratio, and augments ferric ion reduction (FRA) and paraoxonase (PON) activity in a dose-dependent manner. Likewise, MO (particularly at 1.0%) effectively restrained HCD-induced steatosis, hepatic interleukin (IL)-6 production, and protected the kidneys, testes, and ovaries from oxidative stress and cellular senescence. The in silico findings underscore that the six phytoconstituents (chlorogenic acid, isoquercetin, kaempferol 3-O-rutinoside, astragalin, apigetrin, and myricetin) of MO exhibited a strong interaction with HMG-CoA reductase active and binding site residues via hydrogen and hydrophobic interactions. Conclusions: The findings demonstrated an antioxidant, anti-inflammatory, and hypoglycemic effect of MO, guiding the events to prevent HCD-induced metabolic stress and safeguard vital organs. Full article

Excessive use of antibiotic growth promoters (AGPs) in broiler rearing has led to severe issues due to antimicrobial resistance and drug residues in meat. This study was conducted to evaluate aqueous Moringa oleifera leaf extract (MOLE) as a natural alternative to antibiotics in broiler chickens. 150 broiler chicks were randomly distributed into five groups: one control, three MOLE-treated groups (60, 90, and 120 mL/L), and one Enrofloxacin-treated group (an antibiotic). The birds were monitored for a 35-day trial period, split further into a starter phase (0–21 days) and a finisher phase (22–35 days). The results were that at the starter phase of their lives, birds treated with MOLE120 experienced better body weight gain and optimal feed conversion ratio (FCR), which showed improved early growth performance. In the finisher phase, the MOLE90 group demonstrated the best FCR and a favorable weight gain, showing better efficiency at later stages. Crude protein digestibility was highest in the MOLE90 group (69.97%), and apparent metabolizable energy also increased in all MOLE-treated groups, especially MOLE120 (2938.9 kcal/kg). Regarding the blood lipid profile, the MOLE90 group had the lowest low-density lipoprotein (LDL) (82.3 mg/dL) and cholesterol (181.7 mg/dL), while MOLE120 achieved the highest high-density lipoprotein (HDL) level (92.6 mg/dL) with significant (p < 0.05) effects across all parameters. Triglycerides were slightly higher in MOLE groups but remained within physiological limits. In conclusion, MOLE supplementation, particularly at 90–120 mL/L, improved performance and blood lipid metabolism in a phase-specific manner. MOLE120 was more effective in early growth, while MOLE90 proved optimal in the finishing stage. This study supports the potential of MOLE as a phytogenic substitute for antibiotics in poultry production. Full article

Sleep disturbances are linked to metabolic and neurological dysregulation. Moringa oleifera leaves, rich in bioactive compounds, may improve sleep via gut–brain axis modulation. This study investigated the sleep-enhancing effects of fermented Moringa oleifera leaf extract (FM) in mice using metabolomics, gut microbiota analysis, network pharmacology, and molecular docking. A 1:1 combination of Lactobacillus plantarum GDMCC 1.2685 and L. swissii GDMCC 1.791 optimally fermented FM, increasing GABA by 1.67-fold and total amino acids to 46,058.20 ± 845.53 μg/g. FM shortened sleep latency, increased sleep duration, and elevated brain GABA while reducing glutamate (Glu) and Glu/GABA ratios. Hypothalamic metabolomics identified seven sleep-related metabolites, implicating glycerophospholipid, tryptophan, and purine metabolism pathways. FM also reduced Mycobacterium anisopliae (a gut bacterium associated with insomnia) and increased the Firmicutes/Bacteroidetes ratio. Network pharmacology revealed that FM’s effects were mediated via GABA, Glu, and serotonin (5-HT) pathways. These findings demonstrate that FM improves sleep by modulating hypothalamic neurotransmitters and gut microbiota, exerting sedative-hypnotic effects through amino acid, purine, and energy metabolism. Full article

Moringa oleifera leaf (MOL) and their extracts have been demonstrated to possess hypoglycemic effects in a variety of species, but they are still unknown in dogs. This study examined the effects of Moringa oleifera leaf peptide (MOLP) on α-amylase and α-glucosidase activities. Furthermore, we assessed the impact of MOLP on the estimated glycemic index (eGI) of snacks in vitro and the postprandial glycemic response in dogs. The findings indicated that MOLP exhibited significant inhibitory activities against α-amylase (IC₅₀ = 2.29 ± 0.10 mg/mL) and α-glucosidase (IC₅₀ = 2.80 ± 0.04 mg/mL). Moreover, the MOLP-containing snacks exhibited a lower rate of starch hydrolysis during in vitro digestion, leading to a notable reduction in the eGI when compared to white bread (WB) and control snacks. Incorporating MOLP into snacks causes smoother alterations in postprandial blood glucose, significantly reducing glucose peak, time to peak, and glycemic index (GI). Our findings indicate that MOLP exhibits hypoglycemic potential, offering a scientific foundation for the future development of functional foods aimed at managing diabetes in dogs. Full article

Breastfeeding women require specific nutrition to support the quality and secretion of breast milk, which can be achieved through the development of analog rice. Several potential alternatives to develop analog rice, including sago and corn flour, can be developed with the addition of moringa leaf powder due to its high nutritional composition and bioactive compounds, particularly high protein, iron, phytosterols, and flavonoids, which are suitable for breastfeeding women. However, as a new product, besides considering its nutritional value, developing the preferred and acceptable formulation of analog rice remains challenging. This research aims to gain the best formulation and investigate the physicochemical and sensory properties. Three formulations of analog rice were applied in this study utilizing extrusion technology, comprising sago, corn flour, and moringa leaf powder in ratio variations as follows: A, 60:37:3; B, 70:25:5; C, 80:10:10. Overall, the analog rice produced had a green color, a sticky texture, a distinct moringa aroma, and a slightly bitter taste, with Formulation C being most preferred (overall organoleptic value of 2.5, categorized as neutral), containing 6.22 ± 0.83% moisture, 1.04 ± 0.07% ash, 4.08 ± 0.17% protein, 0.46 ± 0.09% fat, 88.21 ± 0.59% carbohydrate, 3.42 ± 1.54% crude fiber, 382.62 ± 3.75 Kcal, 40.12 ± 13.38 ppm iron, 1.09 ± 0.05% sitosterol, 1.16 ± 0.03% stigmasterol, and 0.19 ± 0.07% flavonoid levels. The analog rice provides high energy and lactation-supporting bioactive compounds (iron, phytosterols, and flavonoids), demonstrating potential as a sustainable dietary intervention. This study offers a novel approach through the development of extruded analog rice, which transforms local ingredients into a functional food targeting maternal nutritional gaps by synergizing sago, corn flour, and moringa leaf powder. Full article

Moringa peregrina (Forssk.) Fiori is a tropical tree in southern Iran known as the most important natural coagulant in the world. Today, plant tissue culture is a new method that has a very high potential to produce valuable medicinal compounds on a commercial level. Advances in in vitro cultivation methods have increased the usefulness of plants as renewable resources. In this study, in addition to the phytochemical analysis of the extract of M. peregrina using HPLC, the interaction effect of different concentrations of aqueous extract of M. peregrina (0, 1, 1.5, and 3 mg/L) in two types of MS and ½ MS basal culture media over three weeks on the in vitro growth of Staurogyne repens (Nees) Kuntze was studied. The amounts of quercetin, gallic acid, caffeic acid, and myricetin in the aqueous extract of M. peregrina were 64.9, 374.8, 42, and 4.6 mg/g, respectively. The results showed that using M. peregrina leaf aqueous extract had a positive effect on the length of the branches, the percentage of green leaves, rooting, and the fresh and dry weight of S. repens samples. The highest increase in growth indices was observed in the MS culture medium supplemented with 3 mg/L of M. peregrina leaf aqueous extract after three weeks of cultivation. Of course, this effect was significantly greater in the MS medium and at higher concentrations compared to the ½ MS medium. Three weeks after cultivation at a concentration of 3 mg/L of the extract, the length of the S. repens branches was 5.3 and 1.8 cm in the two basic MS and ½ MS culture media, and the percentage of green leaves was 14 and 4 percent, respectively. Also, rooting was measured at 9.6 and 3.6 percent, fresh weight at 6 and 1.4 g, and dry weight at 1.1 and 0.03 g, respectively. Therefore, adding M. peregrina leaf aqueous extract as a stimulant significantly increased the in vitro growth of S. repens. Full article

Breast milk is the optimal nutrition for infants, yet lactation insufficiency remains a common cause of early breastfeeding cessation. Moringa oleifera has been traditionally used as a galactagogue due to its rich micronutrient and phytosterol content. This systematic review assessed the effects of Moringa leaf supplementation on prolactin levels and breast milk volume in postpartum mothers with lactation insufficiency. A systematic search following PRISMA guidelines, was conducted for randomized controlled trials involving healthy postpartum women supplemented with Moringa oleifera. Risk of bias was evaluated using the Cochrane Risk of Bias Tool. Eight studies met the inclusion criteria, with intervention durations ranged from 3 to 10 days. Moringa supplementation increased significantly breast milk volume by up to 400 mL/day compared to controls. Serum prolactin levels also rose significantly with a mean increase of 231.72 ng/mL Most studies exhibited low to moderate risk of bias, though one study exhibited high risk due to lack of binding and subjective outcome measurement. Moringa oleifera leaf supplementation appears to enhance lactation by increasing milk volume and prolactin levels in postpartum mothers. However, further longer-term studies are needed to establish optimal dosing, sustained effectiveness, and safety. Full article

The increasing global demand for resilient, sustainable agricultural systems has intensified the need for advanced monitoring strategies, particularly for climate-adaptive crops such as Moringa oleifera Lam. This study presents an integrated approach using Unmanned Aerial Vehicles (UAVs) equipped with multispectral and thermal cameras to monitor the vegetative performance and determine the optimal harvest period of four M. oleifera genotypes in a Mediterranean environment. High-resolution data were collected and processed to generate the NDVI, canopy temperature, and height maps, enabling the assessment of plant vigor, stress conditions, and spatial canopy structure. NDVI analysis revealed robust vegetative growth (0.7–0.9), with optimal harvest timing identified on 30 October 2024, when the mean NDVI exceeded 0.85. Thermal imaging effectively discriminated plant crowns from surrounding weeds by capturing cooler canopy zones due to active transpiration. A clear inverse correlation between NDVI and Land Surface Temperature (LST) was observed, reinforcing its relevance for stress diagnostics and environmental monitoring. The results underscore the value of UAV-based multi-sensor systems for precision agriculture, offering scalable tools for phenotyping, harvest optimization, and sustainable management of medicinal and aromatic crops in semiarid regions. Moreover, in this study, to produce M. oleifera leaf powder intended for use as a food ingredient, the leaves of four M. oleifera genotypes were dried, milled, and evaluated for their hygiene and safety characteristics. Plate count analyses confirmed the absence of pathogenic bacterial colonies in the M. oleifera leaf powders, highlighting their potential application as natural and functional additives in food production. Full article