Search Results (946)

Ochratoxin A (OTA), as a mycotoxin, can contaminate a variety of feeds and foods. Existing studies have shown that the main toxicity of OTA to organisms is nephrotoxicity, but the toxic mechanism to other organs is still worthy of further study. Whether OTA causes intestinal damage through the necroptosis pathway mediated by RIPK1/RIPK3/MLKL remains to be elucidated. Astaxanthin (AST), a feed additive with strong antioxidant properties, was used as an antidote to evaluate the alleviation effect on OTA-induced intestinal injury and the underlying mechanism in this research. Chickens are the most sensitive animals to OTA except pigs. Therefore, 70 white-feathered chickens (n = 15) and Chicken Small Intestinal Epithelial Cells (CSIECs) were used as experimental subjects. Experimental models were established by single or combined exposure of OTA (1.0 mg/kg on chickens for 21 d; 2 μM on CSIEC for 24 h) and AST (100 mg/kg on chickens for 21 d; 40 μM on CSIEC for 24 h). In this study, AST significantly ameliorated OTA-induced intestinal damage by restoring the expression of tight junction proteins (Occludin-1, Claudin-1, and ZO-1), attenuating severe histopathological alterations, mitigating the inflammatory response (elevated pro-inflammatory cytokines and reduced anti-inflammatory mediators), and suppressing necroptosis through downregulation of RIPK1, RIPK3 and MLKL expression. Combined evidence from animal experiments and cell culture experiments demonstrated that AST alleviated the necroptosis and inflammation caused by OTA in CSIECs and the intestine of chickens through the RIPK1/RIPK3/MLKL signaling pathway, thereby reducing the damage caused by OTA. Full article

Microalgal biomass has emerged as a valuable and nutrient-rich source of novel plant-based foods of the future, with several demonstrated benefits. In addition to their green and health-promoting characteristics, these foods exhibit bioactive properties that contribute to a range of physiological benefits. Photoautotrophic microalgae are particularly important as a source of food products due to their ability to biosynthesize high-value compounds. Their photosynthetic efficiency and biosynthetic activity are directly influenced by light conditions. The primary goal of this study is to track the changes in the light requirements of various high-value microalgae species and use advanced systems to regulate these conditions. Artificial intelligence (AI) and machine learning (ML) models have emerged as pivotal tools for intelligent microalgal cultivation. This approach involves the continuous monitoring of microalgal growth, along with the real-time optimization of environmental factors and light conditions. By accumulating data through cultivation experiments and training AI models, the development of intelligent microalgae cell factories is becoming increasingly feasible. This review provides a concise overview of the regulatory mechanisms that govern microalgae growth in response to light conditions, explores the utilization of microalgae-based products in plant-based foods, and highlights the potential for future research on intelligent microalgae cultivation systems. Full article

Heavy metal contamination in paddy fields poses serious risks to food safety and crop productivity. This study evaluated the potential of native soil fungi as bioinoculants to reduce metal uptake in rice cultivated under contaminated conditions. Eight fungal strains—four indigenous and four allochthonous—were selected based on their plant growth-promoting traits, including siderophore production and phosphate solubilization. Additional metabolic analysis confirmed the production of bioactive secondary metabolites. In a greenhouse experiment, three rice cultivars were grown under permanent flooding (PF) and alternate wetting and drying (AWD) in soil enriched with arsenic, cadmium, chromium, and copper. Inoculation with indigenous fungi under AWD significantly reduced the arsenic accumulation in rice shoots by up to 75%. While AWD increased cadmium uptake across all cultivars, fungal inoculation led to a moderate reduction in cadmium accumulation—ranging from 15% to 25%—in some varieties. These effects were not observed under PF conditions. The results demonstrate the potential of native fungi as a nature-based solution to mitigate heavy metal stress in rice cultivation, supporting both environmental remediation and sustainable agriculture. Full article

Investigating the volatiles isolated from a commercial spirulina (Arthrospira platensis) dietary supplement by gas chromatography–olfactometry (GC–O) in combination with an aroma extract dilution analysis (AEDA) resulted in 29 odor events with flavor dilution (FD) factors between 8 and 2048. Identification experiments, including various offline and online fractionation approaches, led to the structure assignment of 30 odorants, among which the most potent were sweaty 2- and 3-methylbutanoic acid (FD 2048), roasty, earthy, shrimp-like 2-ethyl-3,5-dimethylpyrazine (FD 2048), vinegar-like acetic acid (FD 1024), and floral, violet-like β-ionone (FD 1024). Static headspace dilution analysis revealed sulfuric, cabbage-like methanethiol (FD factor ≥ 32) as an additional potent odorant. In summary, 31 important spirulina odorants were identified in this study, and 14 were reported for the first time as spirulina constituents. Our data will provide a basis for future odor optimization of spirulina-based food products. Full article

Background: Breastfeeding is essential for preventing non-communicable diseases. However, mothers with chronic illnesses tend to breastfeed less, increasing the likelihood of abandoning breastfeeding, especially if they experience gestational complications. Objective: To analyze the association between factors such as prepregnancy maternal characteristics, gestational complications, food security, barriers, and facilitators with the practice of exclusive breastfeeding. Methods: Cross-sectional study with 566 women who had prenatal care and gave birth at the National Institute of Perinatology (Mexico City) between 2021 and 2024. Surveys were administered on breastfeeding practices, food insecurity, barriers, and facilitators of exclusive breastfeeding in mothers. In addition, sociodemographic information, medical history (prepregnancy conditions and complications), gestational weight gain, and neonatal outcomes were recorded. Results: Of the 566 women, only 43.6% practiced exclusive breastfeeding, with a median duration of 4 months. Exclusive breastfeeding was more frequent in young, stay-at-home mothers with lower educational attainment and in those with food insecurity, who also tended to delay the introduction of complementary foods until after six months. Prepregnancy risk conditions (OR 1.56, 95% CI 1.06–2.30) and multiparity (OR 1.64, 95% CI 1.08–2.49) increased the risk of non-exclusive breastfeeding. Conversely, food insecurity (OR 0.40, 95% CI 0.20–0.78) and counseling from healthcare personnel (OR 0.09, 95% CI 0.01–0.51) showed a protective effect. The analysis also showed that paid employment (OR 4.68, 95% CI 1.65–13.21), the perception of low milk production (OR 6.45, 95% CI 2.95–14.10), maternal illness/medication (OR 3.91, 95% CI 1.36–11.28), and fatigue (OR 4.67, 95% CI 1.36–11.28) increased the probability of non-exclusive breastfeeding. Conclusions: In Mexico, the promotion of exclusive breastfeeding faces challenges, especially in mothers who begin pregnancy with significant chronic conditions such as diabetes, hypertension, obesity, advanced maternal age, and hypothyroidism, among others. Healthcare personnel should provide personalized advice to each woman from the prenatal stage on strategies to achieve and maintain exclusive breastfeeding. Full article

Computer Vision (CV) has proven to be a powerful tool for automation in agri-food industrial processes, offering high-precision solutions tailored to specific working conditions. Recent advancements in Artificial Neural Networks (ANNs) have revolutionized CV applications, enabling systems to autonomously learn and optimize tasks. However, ANN-based approaches often require complex development and lengthy training periods, making their implementation a challenge. In this study, we explore the use of the Segment Anything Model (SAM), a pre-trained neural network developed by META AI in 2023, as an alternative for industrial segmentation tasks in the table olive (Olea europaea L.) processing industry. SAM’s ability to segment objects regardless of scene composition makes it a promising tool to improve the efficiency of olive pitting machines (DRRs). These machines, widely employed in industrial processing, frequently experience mechanical inefficiencies, including the “boat error,” which arises when olives are improperly oriented, leading to defective pitting and pit splinter contamination. Our approach integrates SAM into n CV workflow to diagnose and quantify boat errors without designing or training an additional task-specific ANN. By analyzing the segmented images, we can determine both the percentage of boat errors and the size distribution of olives during transport. The results validate SAM as a feasible option for industrial segmentation, offering a simpler and more accessible solution compared to traditional ANN-based methods. Moreover, our statistical analysis reveals that improper calibration—manifested as size deviations from the nominal value—does not significantly increase boat error rates. This finding supports the adoption of complementary CV technologies to enhance olive pitting efficiency. Future work could investigate real-time integration and the combination of CV with electromechanical correction systems to fully automate and optimize the pitting process. Full article

Many meals are consumed in public settings such as schools, hospitals, and elderly care. These meals have the potential to impact public health as well as promote sustainable lifestyles. However, gastronomic values can be important for loading the meals with other dimensions. The aim of this article is to explore how the concept of gastronomy is understood, used, and described in the scientific literature in the context of public meals. For this scoping review, a systematic approach was adopted, including articles that use the concept of gastronomy in the context of public meals, as defined in this paper. A total of 3676 articles were initially identified, of which eight were finally included for assessment in this review. Three main themes emerged in the analysis, illustrating how the concept of gastronomy was used: gastronomy as local food and national cuisine; gastronomy as ideas of ‘the good’; and gastronomy as a framework. The concept of gastronomy is seldom clearly defined but is often used to emphasize ideas of ‘the good’, local food, and culinary traditions. Through gastronomy, healthy and sustainable public meals can be loaded with additional value, creativity, and innovation, enhancing the overall meal experience. Full article

Three-dimensional food printing (3DFP), a specialized application of additive manufacturing (AM), employs a layer-by-layer deposition process guided by digital image files to fabricate edible structures. Utilizing heavily modified 3D printers and Computer-Aided Design (CAD) software technology allows for the precise creation of customized food items tailored to individual aesthetic preferences and nutritional requirements. Three-dimensional food printing holds significant potential in revolutionizing the food industry by enabling the production of personalized meals, enhancing the sensory dining experience, and addressing specific dietary constraints. Despite these promising applications, 3DFP remains one of the most intricate and technically demanding areas within AM, particularly in the context of modern gastronomy. Challenges such as the rheological behaviour of food materials, print stability, and the integration of cooking functions must be addressed to fully realize its capabilities. This article explores the possibilities of applying classical modified 3D printers in the food industry. The behaviour of certain recipes is also tested. Two test case scenarios are covered. The first scenario is the work and formation of a homogenized meat mass. The second scenario involves finding a chocolate recipe that is suitable for printing relatively detailed chocolate decorative elements. The current advancements, technical challenges, and future opportunities of 3DFP in the field of engineering, culinary innovation and nutritional science are also explored. Full article

Accurate and timely monitoring of plant nitrogen content (PNC) is essential for precision agriculture (PA) and food security. While multispectral unmanned aerial vehicle (UAV) imagery has shown promise in PNC estimation, the optimal feature combination methods of spectral and texture features remain underexplored, and model transferability across different agricultural practices is poorly understood. This study aims to present an innovative approach by integrating 40 texture features and 22 spectral features from UAV multispectral images with machine learning (ML) methods (RF, SVR, and XGBoost) for winter wheat nitrogen content prediction. In addition, through analysis of an 8-year long-term field experiment with rigorous data, the results indicated that (1) the RF and XGboost models incorporating both spectral and texture features achieved good prediction accuracy, with R² values of 0.98 and 0.99, respectively, RMSE values of 0.10 and 0.07, and MAE values of 0.07and 0.05; (2) models trained on Farmers’ Practice (FP) data showed superior transferability to Ecological Intensification (EI) conditions (R² = 0.98, RMSE = 0.08, and MAE = 0.05 for XGBoost), while EI-trained models performed less well when applied to FP conditions (R² = 0.89, RMSE = 0.45, and MAE = 0.35 for XGBoost). These findings established an effective framework for UAV-based PNC monitoring, demonstrating that fused spectral–textural features with FP-trained XGboost can achieve both high accuracy and practical transferability, offering valuable decision-support tools for precision nitrogen management in different farming systems. Full article

2,3,5-Trimethylpyrazine (TMP) is an alkyl pyrazine with broad application prospects in the fields of food additives and medicine. L-threonine-3-dehydrogenase (TDH) is a key enzyme in the biosynthesis pathway of TMP. To explore the efficient and environmentally friendly production method of TMP, we constructed recombinant strains overexpressing the BlTDH gene and its mutant BlTDH (N157A) using Bacillus licheniformis YC7. The TMP yield of recombinant strains with pHT01-BlTDH (N157A) reached 15.35 ± 1.51 mg/L, which was significantly higher than that of strains with pHT01-BlTDH (9.86 ± 1.24 mg/L) and strains with vector pHT01 (2.35 ± 0.58 mg/L). To further increase the TMP yield of strain YC7/pHT01-BlTDH (N157A), the fermentation process was optimized by single-factor experiments, and the response surface test was conducted using the Box–Behnken design. The results revealed that the substrate ratio, IPTG concentration and fermentation time had significant effects on the yield of TMP, and the degree of influence was substrate ratio > fermentation time > IPTG concentration. The optimization results of response surface indicated that the optimal fermentation conditions were as follows: substrate ratio of 1:2, IPTG concentration of 1.0 mM, and fermentation time of 4 d. Under these conditions, the TMP yield reached 44.52 ± 0.21 mg/L, which was around 0.005 mg/L different from the predicted value (45.515 mg/L), and increased by 29.17 mg/L compared with the initial condition. The optimization of fermentation conditions significantly increased the yield of TMP produced by recombinant strains, which provided a theoretical basis and strain resources for industrial production of TMP. Full article

With the increasing accumulation of plastic residues in agricultural ecosystems, microplastics (MPs) have emerged as a novel and pervasive environmental risk factor threatening sustainable agriculture. Compared to aquatic systems, our understanding of MP dynamics in agricultural soils—particularly their transport mechanisms, bioavailability, plant uptake pathways, and ecological impacts—remains limited. These knowledge gaps impede accurate risk assessment and hinder the development of effective mitigation strategies. This review critically synthesises current knowledge in the study of MPs within soil–plant systems. It examines how MPs influence soil physicochemical properties, plant physiological processes, toxicological responses, and rhizosphere interactions. It further explores the transport dynamics of MPs in soil–plant systems and recent advances in analytical techniques for their detection and quantification. The role of plant functional traits in mediating species-specific responses to MP exposure is also discussed. In addition, the review evaluates the ecological relevance of laboratory-based findings under realistic agricultural conditions, highlighting the methodological limitations imposed by pollution heterogeneity, complex exposure scenarios, and detection technologies. It also examines existing policy responses at both regional and global levels aimed at addressing MP pollution in agriculture. To address these challenges, we propose future research directions that include the integration of multi-method detection protocols, long-term and multi-site field experiments, the development of advanced risk modelling frameworks, and the establishment of threshold values for MP residues in edible crops. Additionally, we highlight the need for future policies to regulate the full life cycle of agricultural plastics, monitor soil MP residues, and integrate MP risks into food safety assessments. This review provides both theoretical insights and practical strategies for understanding and mitigating MP pollution in agroecosystems, supporting the transition toward more sustainable, resilient, and environmentally sound agricultural practices. Full article

This study aims to identify the chemical structure and immunomodulatory activity of exopolysaccharides (EPSs) from Acetilactobacillus jinshanensis BJ01 and suggest its potential applications in the pharmaceutical field and as functional food additives. The EPS-1 produced by A. jinshanensis BJ01 was purified using column chromatography. The lyophilized powder obtained by vacuum freeze-drying was used for structural characterization and immunomodulatory activity analysis. Gel permeation chromatography (GPC) determined its molecular weight as 156.58 kDa. High-performance anion-exchange chromatography (HPAEC) identified that the EPS-1 is composed of mannose, xylose, and glucose. The structural characterization of EPS-1 by gas chromatography–mass spectrometry (GC-MS) and 1D/2D nuclear magnetic resonance (NMR) spectroscopy demonstrated that EPS-1 is primarily composed of α-D-Manp-(1→, →2,6)-α-D-Manp-(1→, →2)-α-D-Manp-(1→, and →3)-α-D-Manp-(1→. Scanning electron microscopy (SEM) and atomic force microscopy (AFM) illustrated that EPS-1 exhibited a round, flake-like morphology. In vitro experiments with RAW264.7 macrophages demonstrated the high immunomodulatory activity of EPS-1. Significant upregulation of iNOS, IL-6, and TNF-α mRNA levels was confirmed by qRT-PCR (p < 0.05). Western blotting revealed that EPS-1 (6.25 μg/mL) induced phosphorylation of NF-κB (p65, IκBα) and MAPK (ERK) signaling proteins. This study provides the first structural and immunomodulatory characterization of an exopolysaccharide from A. jinshanensis BJ01, highlighting its potential as a novel immune adjuvant. Full article

Ultraprocessed foods (UPFs), now dominant in global diets, pose health risks that go beyond poor nutrition due to the synergistic effects of compounds in their ultracomplex industrial formulations. This narrative review aims to provide researchers and health professionals in the field of cancer with updated and critical information, as they are often unaware of the complex and evolving evidence linking UPFs to carcinogenesis. The review discusses potential mechanisms through which UPFs may contribute to cancer development, including harmful additives, neo-formed contaminants, and packaging-derived substances, as well as the displacement of protective nutrients found in whole foods. Despite limitations in establishing direct causality, epidemiological studies consistently associate high UPF intake with increased incidence of various cancers, notably colorectal, breast, and pancreatic cancers. These findings reflect a broader paradigm shift in nutritional epidemiology, recognizing that food processing is an essential dimension of diet-related health risks. To mitigate the impact of UPFs, the review emphasizes the need for preventive strategies that integrate clear dietary guidelines, regulatory measures on food labeling and additives, and public education campaigns. Successful international experiences in regulating marketing and improving transparency serve as important references. Moreover, eliminating corporate influence and conflicts of interest is crucial to ensure that public health, rather than industry agendas, guides nutrition policy. As scientific research advances to clarify the mechanisms of action and synergistic effects of harmful compounds in UPFs, coordinated efforts are needed to reduce their consumption and ultimately alleviate the global cancer burden. Full article

Municipal solid waste management (MSWM) on islands involves several challenges relating to politics, society, the environment, and technology. This paper addresses the potential for producing biogas and biomethane from food waste on Tenerife, including waste from households, with the aim of reducing landfill and primary fossil energy consumption. The study also introduces the European and Regional policy framework and requirements. Effective microorganisms have been studied as proposals to stabilise the food waste from households, avoiding odours and decomposition during storage. The trials show positive results in terms of the preservation of organic matter until the food waste is transported to the biogas plant. In addition, a new concept for a small biogas plant made of textile materials, which are suited to the municipalities of Tenerife, is presented to provide an easy-to-build solution, with ranges of up to 75 kW in electrical power. With a theoretical potential of 299,012 tons of food waste being available per year (based on 2022), preliminary laboratory experiments with real samples of the island showed a theoretical potential of 28.97 × 10⁶ Nm³ for biogas and 264,612 tons for digestate, which can be used as fertilisers, with potential savings of 18.15 × 10⁶ L of gasoline and 42.66 × 10³ equivalent CO₂ tons. Full article

Soil salinization presents a significant challenge, driven by factors such as inadequate drainage, shallow aquifers, and high evaporation rates, threatening global food security. The sunflower emerges as a key cash crop in such areas, providing the opportunity to convert its straw into biochar, which offers additional agronomic and environmental benefits. This study investigates the effectiveness of biochar interlayers in enhancing salt leaching and suppressing upward salt migration through integrated laboratory and field experiments. The effectiveness of varying biochar interlayer application rates was assessed in promoting salt leaching, decreasing soil electrical conductivity (EC), and enhancing crop performance in saline soils through a systematic approach that combines laboratory and field experiments. The biochar treatments included a control (CK) and different applications of 20 (BL20), 40 (BL40), 60 (BL60), and 80 (BL80) tons of biochar per hectare, all applied below a depth of 20 cm, with each treatment replicated three times. The laboratory and field experimental setups maintained consistency in terms of biochar treatments and interlayer placement methodology. During the laboratory column experiments, the soil columns were treated with deionized water, and their leachates were analyzed for EC and major ionic components. The results showed that columns with biochar interlayers exhibited significantly higher efflux rates compared to those of the control and notably accelerated the time required for the effluent EC to decrease to 2 dS m⁻¹. The CK required 43 days for full discharge and 38 days for EC stabilization below 2 dS m⁻¹. In contrast, biochar treatments notably reduced these times, with BL80 achieving discharge in just 7 days and EC stabilization in 10 days. Elution events occurred 20–36 days earlier in the biochar-treated columns, confirming biochar’s effectiveness in enhancing leaching efficiency in saline soils. The field experiment results supported the laboratory findings, indicating that increased biochar application rates significantly reduced soil EC and ion concentrations at depths of 0–20 cm and 20–40 cm, lowering the EC from 7.12 to 2.25 dS m⁻¹ and from 6.30 to 2.41 dS m⁻¹ in their respective layers. The application of biochar interlayers resulted in significant reductions in Na⁺, K⁺, Ca²⁺, Mg²⁺, Cl⁻, SO₄²⁻, and HCO₃⁻ concentrations across both soil layers. In the 0–20 cm layer, Na⁺ decreased from 3.44 to 2.75 mg·g⁻¹, K⁺ from 0.24 to 0.11 mg·g⁻¹, Ca²⁺ from 0.35 to 0.20 mg·g⁻¹, Mg²⁺ from 0.31 to 0.24 mg·g⁻¹, Cl⁻ from 1.22 to 0.88 mg·g⁻¹, SO₄²⁻ from 1.91 to 1.30 mg·g⁻¹ and HCO₃⁻ from 0.39 to 0.18 mg·g⁻¹, respectively. Similarly, in the 20–40 cm layer, Na⁺ declined from 3.62 to 3.05 mg·g⁻¹, K⁺ from 0.28 to 0.12 mg·g⁻¹, Ca²⁺ from 0.39 to 0.26 mg·g⁻¹, Mg²⁺ from 0.36 to 0.27 mg·g⁻¹, Cl⁻ from 1.18 to 0.80 mg·g⁻¹, SO₄²⁻ from 1.95 to 1.33 mg·g⁻¹ and HCO₃⁻ from 0.42 to 0.21 mg·g⁻¹ under increasing biochar rates. Moreover, the use of biochar interlayers significantly improved the physiological traits of sunflowers, including their photosynthesis rates, stomatal conductance, and transpiration efficiency, thereby boosting biomass and achene yield. These results highlight the potential of biochar interlayers as a sustainable strategy for soil desalination, water conservation, and enhanced crop productivity. This approach is especially promising for managing salt-affected soils in regions like California, where soil salinization represents a considerable threat to agricultural sustainability. Full article