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13 pages, 3573 KiB  
Article
The Effects of Pruning Date on Flowering, Yield, and Fruit Quality of ‘Korean White’ Pitaya (Selenicereus undatus [(Haw.) Britton and Rose]) Cultivated in Unheated Greenhouses of Southeast Spain
by Ramón Rodríguez-Garrido, Fernando M. Chiamolera and Julián Cuevas
Horticulturae 2025, 11(8), 919; https://doi.org/10.3390/horticulturae11080919 (registering DOI) - 5 Aug 2025
Abstract
Pitaya (Selenicereus undatus) is a long-day climbing cactus that blooms in waves mostly on 1-year old, succulent leafless shoots called cladodes. Nonetheless, pitaya can also bloom on new-year growth if the buds of the cladodes are mature enough and competent for [...] Read more.
Pitaya (Selenicereus undatus) is a long-day climbing cactus that blooms in waves mostly on 1-year old, succulent leafless shoots called cladodes. Nonetheless, pitaya can also bloom on new-year growth if the buds of the cladodes are mature enough and competent for flower induction. Here, we tested, during two consecutive years, whether early pruning could have a positive effect on promoting more flowering waves, better fruiting, and heavier yield of ‘Korean White’ pitaya cultivated in unheated greenhouses of Southeastern Spain. The results show that pruning in January instead of March did not consistently modify the reproductive behavior of ‘Korean White’ pitaya in our conditions. Therefore, no significant effects on the number of blooming waves, flowering intensity, fruit set, quality or yield were observed. The only positive effect, not always significant, was an increase in fruit size that led to better fruit distribution into commercial categories in one out of the two experimental seasons. The lack of effect of early pruning was attributed to the prevalent low temperatures during winter in Spain. The results, however, suggest it is worthwhile exploring whether greenhouse heating with temperatures above pitaya’s base temperature may have the desired effects on increasing blooming waves. The profitability of this practice have to be carefully assessed. Full article
(This article belongs to the Special Issue Orchard Management: Strategies for Yield and Quality)
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23 pages, 1298 KiB  
Article
Evaluation of the Quality and Nutritional Value of Modified Corn Wet Distillers’ Grains Plus Solubles (mcWDGS) Preserved in Aerobic and Anaerobic Conditions
by Mateusz Roguski, Marlena Zielińska-Górska, Andrzej Radomski, Janusz Zawadzki, Marlena Gzowska, Anna Rygało-Galewska and Andrzej Łozicki
Sustainability 2025, 17(15), 7097; https://doi.org/10.3390/su17157097 - 5 Aug 2025
Abstract
To enhance the effectiveness of sustainable preservation of modified corn wet distillers’ grains plus solubles (mcWDGS), various additives were tested under aerobic and anaerobic conditions. In Experiment I, the mcWDGS was stored under aerobic conditions for 5 days at 25 °C. Treatments included [...] Read more.
To enhance the effectiveness of sustainable preservation of modified corn wet distillers’ grains plus solubles (mcWDGS), various additives were tested under aerobic and anaerobic conditions. In Experiment I, the mcWDGS was stored under aerobic conditions for 5 days at 25 °C. Treatments included different organic acids applied at 0.3% or 0.6% of fresh matter (FM). In Experiment II, the mcWDGS was ensiled anaerobically for 8 weeks at 25 °C using organic acids, a commercial acid mixture, or a microbial inoculant at 0.2% FM. In aerobic conditions, the best preservability was achieved with propionic and formic acids at 0.6% FM, as indicated by the lowest temperature, pH, and microbial counts on days 3 and 5 (p ≤ 0.01). Under anaerobic storage, the highest lactic acid concentrations were recorded in the control, citric acid, and commercial acid mixture variants (p ≤ 0.01). Acetic acid levels were highest in the control (p ≤ 0.01). The highest NH3-N content was found in the formic acid variant and the lowest in the inoculant variant (p ≤ 0.01). Aerobic stability after ensiling was greatest in the control and propionic acid groups (p ≤ 0.01). Nutritional analysis showed that the citric acid group had the highest dry matter content (p ≤ 0.01), while the control group contained the most crude protein (p ≤ 0.01) and saturated fatty acids (p ≤ 0.05). The propionic acid and commercial acid mixture variants had the highest unsaturated fatty acids (p ≤ 0.05). Antioxidant capacity was also greatest in the control (p ≤ 0.01). In conclusion, mcWDGS can be effectively preserved aerobically with 0.6% FM of propionic or formic acid, and anaerobically via ensiling, even without additives. These findings support its potential as a stable and nutritious feed ingredient. Full article
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14 pages, 10994 KiB  
Article
Novel Cemented Carbide Inserts for Metal Grooving Applications
by Janusz Konstanty, Albir Layyous and Łukasz Furtak
Materials 2025, 18(15), 3674; https://doi.org/10.3390/ma18153674 - 5 Aug 2025
Abstract
Although cemented carbides have been manufactured by the powder metallurgy (P/M) technology for over a century now, systematic developmental efforts are still underway. In the present study, tool life improvements in metal grooving applications are the key objective. Four PVD-coated cemented carbides compositions, [...] Read more.
Although cemented carbides have been manufactured by the powder metallurgy (P/M) technology for over a century now, systematic developmental efforts are still underway. In the present study, tool life improvements in metal grooving applications are the key objective. Four PVD-coated cemented carbides compositions, dedicated to groove steel, stainless steel, cast iron, and aluminium alloys, have been newly designed, along with their manufacturing conditions. Physical, mechanical and chemical characteristics—such as sintered density, modulus of elasticity, hardness, fracture toughness, WC grain size, and the chemical composition of the substrate material, as well as the chemical composition, microhardness, structure, and thickness of the coatings—have been studied. A series of grooving tests have also been conducted to assess whether modifications to the thus far marketed tool materials, tool geometries, and coatings can improve cutting performance. In order to compare the laboratory and application properties of the investigated materials with currently produced by reputable companies, commercial inserts have also been tested. The experimental results obtained indicate that the newly developed grooving inserts exhibit excellent microstructural characteristics, high hardness, fracture toughness, and wear resistance and that they show slightly longer tool life compared to the commercial ones. Full article
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15 pages, 4075 KiB  
Article
Biological Characteristics and Domestication of a Wild Hericium coralloides
by Ji-Ling Song, Ya Xin, Zu-Fa Zhou, Xue-Ping Kang, Yang Zhang, Wei-Dong Yuan and Bin Yu
Horticulturae 2025, 11(8), 917; https://doi.org/10.3390/horticulturae11080917 (registering DOI) - 5 Aug 2025
Abstract
Hericium coralloides is a highly valued gourmet and medicinal species with growing market demand across East Asia, though industrial production remains limited by cultivation challenges. This study investigated the molecular characteristics, biological traits, domestication potential, and cultivation protocols of Hericium coralloides strains collected [...] Read more.
Hericium coralloides is a highly valued gourmet and medicinal species with growing market demand across East Asia, though industrial production remains limited by cultivation challenges. This study investigated the molecular characteristics, biological traits, domestication potential, and cultivation protocols of Hericium coralloides strains collected from the Changbaishan Nature Reserve (Jiling, China). Optimal conditions for mycelial growth included mannose as the preferred carbon source, peptone as the nitrogen source, 30 °C incubation temperature, pH 5.5, and magnesium sulfate as the essential inorganic salt. The fruiting bodies had a protein content of 2.43% g/100 g (fresh sample meter). Total amino acids comprised 53.3% of the total amino acid profile, while essential amino acids accounted for 114.11% relative to non-essential amino acids, indicating high nutritional value. Under optimized domestication conditions—70% hardwood chips, 20% cottonseed hulls, 8% bran, 1% malic acid, and 1% gypsum—bags reached full colonization in 28 days, with a 15-day maturation phase and initial fruiting occurring after 12–14 days. The interval between flushes was 10–12 days. The average yield reached 318.65 ± 31.74 g per bag, with a biological conversion rate of 63.73%. These findings demonstrate that Hericium coralloides possesses significant potential for edible and commercial applications. This study provides a robust theoretical foundation and resource reference for its artificial cultivation, supporting its broader industrial and economic utilization. Full article
(This article belongs to the Special Issue Advances in Propagation and Cultivation of Mushroom)
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23 pages, 5970 KiB  
Review
Practical Review on Aetio-Pathogenesis and Symptoms in Pigs Affected by Clinical and Subclinical Oedema Disease and the Use of Commercial Vaccines Under Field Conditions
by Juan Hernandez-Garcia, Isaac Ballarà Rodriguez, Ramon Jordà Casadevall, Sergi Bruguera, David Llopart and Emili Barba-Vidal
Animals 2025, 15(15), 2275; https://doi.org/10.3390/ani15152275 - 4 Aug 2025
Abstract
The impact of Oedema Disease produced by Shiga toxigenic Escherichia coli (STEC) in swine is increasing in some production countries due to increasing limitations on treatment with antimicrobials and zinc oxide, either because of the increased prevalence of multi-resistant strains or because of [...] Read more.
The impact of Oedema Disease produced by Shiga toxigenic Escherichia coli (STEC) in swine is increasing in some production countries due to increasing limitations on treatment with antimicrobials and zinc oxide, either because of the increased prevalence of multi-resistant strains or because of legal restrictions. The main pathological effect of Shiga toxin 2e is represented by damage to the endothelial cells of the blood vessel walls, leading to liquid extravasation and oedema formation in multiple tissues. These oedemas are generally easily identifiable in acute clinical cases. However, disease caused by Shiga toxin can occur without any externally visible oedema in the pigs, as observed in the subclinical presentation of Oedema Disease. It also causes productive losses, so it is important to identify and/or diagnose cases to set up control measures in order to optimize production and health. This article includes a comprehensive review of lesions and signs caused by Shiga toxin toxicosis in pigs, as well as other insights about the aetiology and epidemiology of STEC in pigs, and the effect of Shiga toxin recombinant toxoid vaccines in reducing these clinical and subclinical signs under field conditions. Full article
(This article belongs to the Section Pigs)
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33 pages, 3972 KiB  
Article
A Review and Case of Study of Cooling Methods: Integrating Modeling, Simulation, and Thermal Analysis for a Model Based on a Commercial Electric Permanent Magnet Synchronous Motor
by Henrry Gabriel Usca-Gomez, David Sebastian Puma-Benavides, Victor Danilo Zambrano-Leon, Ramón Castillo-Díaz, Milton Israel Quinga-Morales, Javier Milton Solís-Santamaria and Edilberto Antonio Llanes-Cedeño
World Electr. Veh. J. 2025, 16(8), 437; https://doi.org/10.3390/wevj16080437 - 4 Aug 2025
Abstract
The efficiency of electric motors is highly dependent on their operating temperature, with lower temperatures contributing to enhanced performance, reliability, and extended service life. This study presents a comprehensive review of state-of-the-art cooling technologies and evaluates their impact on the thermal behavior of [...] Read more.
The efficiency of electric motors is highly dependent on their operating temperature, with lower temperatures contributing to enhanced performance, reliability, and extended service life. This study presents a comprehensive review of state-of-the-art cooling technologies and evaluates their impact on the thermal behavior of a commercial motor–generator system in high-demand applications. A baseline model of a permanent magnet synchronous motor (PMSM) was developed using MotorCAD 2023® software, which was supported by reverse engineering techniques to accurately replicate the motor’s physical and thermal characteristics. Subsequently, multiple cooling strategies were simulated under consistent operating conditions to assess their effectiveness. These strategies include conventional axial water jackets as well as advanced oil-based methods such as shaft cooling and direct oil spray to the windings. The integration of these systems in hybrid configurations was also explored to maximize thermal efficiency. Simulation results reveal that hybrid cooling significantly reduces the temperature of critical components such as stator windings and permanent magnets. This reduction in thermal stress improves current efficiency, power output, and torque capacity, enabling reliable motor operation across a broader range of speeds and under sustained high-load conditions. The findings highlight the effectiveness of hybrid cooling systems in optimizing both thermal management and operational performance of electric machines. Full article
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20 pages, 3145 KiB  
Article
Determination of Dynamic Elastic Properties of 3D-Printed Nylon 12CF Using Impulse Excitation of Vibration
by Pedro F. Garcia, Armando Ramalho, Joel C. Vasco, Rui B. Ruben and Carlos Capela
Polymers 2025, 17(15), 2135; https://doi.org/10.3390/polym17152135 - 4 Aug 2025
Abstract
Material Extrusion (MEX) process is increasingly used to fabricate components for structural applications, driven by the availability of advanced materials and greater industrial adoption. In these contexts, understanding the mechanical performance of printed parts is crucial. However, conventional methods for assessing anisotropic elastic [...] Read more.
Material Extrusion (MEX) process is increasingly used to fabricate components for structural applications, driven by the availability of advanced materials and greater industrial adoption. In these contexts, understanding the mechanical performance of printed parts is crucial. However, conventional methods for assessing anisotropic elastic behavior often rely on expensive equipment and time-consuming procedures. The aim of this study is to evaluate the applicability of the impulse excitation of vibration (IEV) in characterizing the dynamic mechanical properties of a 3D-printed composite material. Tensile tests were also performed to compare quasi-static properties with the dynamic ones obtained through IEV. The tested material, Nylon 12CF, contains 35% short carbon fibers by weight and is commercially available from Stratasys. It is used in the fused deposition modeling (FDM) process, a Material Extrusion technology, and exhibits anisotropic mechanical properties. This is further reinforced by the filament deposition process, which affects the mechanical response of printed parts. Young’s modulus obtained in the direction perpendicular to the deposition plane (E33), obtained via IEV, was 14.77% higher than the value in the technical datasheet. Comparing methods, the Young’s modulus obtained in the deposition plane, in an inclined direction of 45 degrees in relation to the deposition direction (E45), showed a 22.95% difference between IEV and tensile tests, while Poisson’s ratio in the deposition plane (v12) differed by 6.78%. This data is critical for designing parts subject to demanding service conditions, and the results obtained (orthotropic elastic properties) can be used in finite element simulation software. Ultimately, this work reinforces the potential of the IEV method as an accessible and consistent alternative for characterizing the anisotropic properties of components produced through additive manufacturing (AM). Full article
(This article belongs to the Special Issue Mechanical Characterization of Polymer Composites)
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28 pages, 3909 KiB  
Article
Exploring How Climate Change Scenarios Shape the Future of Alboran Sea Fisheries
by Isabella Uzategui, Susana Garcia-Tiscar and Paloma Alcorlo
Water 2025, 17(15), 2313; https://doi.org/10.3390/w17152313 - 4 Aug 2025
Abstract
Climate change is disrupting marine ecosystems, necessitating a deeper understanding of environmental and fishing-related impacts on exploited species. This study examines the effects of physical factors (temperature, thermal anomalies, salinity, seabed conditions), biogeochemical elements (pH, oxygen levels, nutrients, primary production), and fishing pressure [...] Read more.
Climate change is disrupting marine ecosystems, necessitating a deeper understanding of environmental and fishing-related impacts on exploited species. This study examines the effects of physical factors (temperature, thermal anomalies, salinity, seabed conditions), biogeochemical elements (pH, oxygen levels, nutrients, primary production), and fishing pressure on the biomass of commercially important species in the Alboran Sea from 1999 to 2022. Data were sourced from the Copernicus observational program, focusing on the geographical sub-area 1 (GSA-1) zone across three depth ranges. Generalized Additive Models were applied for analysis. Rising temperatures and seasonal anomalies have largely negative effects, disrupting species’ physiological balance. Changes in water quality, including improved nutrient and oxygen concentrations, have yielded complex ecological responses. Fishing indices highlight the vulnerability of small pelagic fish to climate change and overfishing, underscoring their economic and ecological significance. These findings stress the urgent need for ecosystem-based management strategies that integrate climate change impacts to ensure sustainable marine resource management. Full article
(This article belongs to the Special Issue Impact of Climate Change on Marine Ecosystems)
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27 pages, 2361 KiB  
Review
Review of Thrust Regulation and System Control Methods of Variable-Thrust Liquid Rocket Engines in Space Drones
by Meng Sun, Xiangzhou Long, Bowen Xu, Haixia Ding, Xianyu Wu, Weiqi Yang, Wei Zhao and Shuangxi Liu
Actuators 2025, 14(8), 385; https://doi.org/10.3390/act14080385 - 4 Aug 2025
Abstract
Variable-thrust liquid rocket engines are essential for precision landing in deep-space exploration, reusable launch vehicle recovery, high-accuracy orbital maneuvers, and emergency obstacle evasions of space drones. However, with the increasingly complex space missions, challenges remain with the development of different technical schemes. In [...] Read more.
Variable-thrust liquid rocket engines are essential for precision landing in deep-space exploration, reusable launch vehicle recovery, high-accuracy orbital maneuvers, and emergency obstacle evasions of space drones. However, with the increasingly complex space missions, challenges remain with the development of different technical schemes. In view of these issues, this paper systematically reviews the technology’s evolution through mechanical throttling, electromechanical precision regulation, and commercial space-driven deep throttling. Then, the development of key variable thrust technologies for liquid rocket engines is summarized from the perspective of thrust regulation and control strategy. For instance, thrust regulation requires synergistic flow control devices and adjustable pintle injectors to dynamically match flow rates with injection pressure drops, ensuring combustion stability across wide thrust ranges—particularly under extreme conditions during space drones’ high-maneuver orbital adjustments—though pintle injector optimization for such scenarios remains challenging. System control must address strong multivariable coupling, response delays, and high-disturbance environments, as well as bottlenecks in sensor reliability and nonlinear modeling. Furthermore, prospects are made in response to the research progress, and breakthroughs are required in cryogenic wide-range flow regulation for liquid oxygen-methane propellants, combustion stability during deep throttling, and AI-based intelligent control to support space drones’ autonomous orbital transfer, rapid reusability, and on-demand trajectory correction in complex deep-space missions. Full article
(This article belongs to the Section Aerospace Actuators)
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18 pages, 674 KiB  
Article
Oil Extraction Systems Influence the Techno-Functional and Nutritional Properties of Pistachio Processing By-Products
by Rito J. Mendoza-Pérez, Elena Álvarez-Olmedo, Ainhoa Vicente, Felicidad Ronda and Pedro A. Caballero
Foods 2025, 14(15), 2722; https://doi.org/10.3390/foods14152722 - 4 Aug 2025
Abstract
Low-commercial-value natural pistachios (broken, closed, or immature) can be revalorised through oil extraction, obtaining a high-quality oil and partially defatted flour as by-product. This study evaluated the techno-functional and nutritional properties of the flours obtained by hydraulic press (HP) and single-screw press (SSP) [...] Read more.
Low-commercial-value natural pistachios (broken, closed, or immature) can be revalorised through oil extraction, obtaining a high-quality oil and partially defatted flour as by-product. This study evaluated the techno-functional and nutritional properties of the flours obtained by hydraulic press (HP) and single-screw press (SSP) systems, combined with pretreatment at 25 °C and 60 °C. The extraction method significantly influenced flour’s characteristics, underscoring the need to tailor processing conditions to the specific technological requirements of each food application. HP-derived flours presented lighter colour, greater tocopherol content, and higher water absorption capacity (up to 2.75 g/g), suggesting preservation of hydrophilic proteins. SSP-derived flours showed higher concentration of protein (44 g/100 g), fibre (12 g/100 g), and minerals, and improved emulsifying properties, enhancing their suitability for emulsified products. Pretreatment at 25 °C enhanced functional properties such as swelling power (~7.0 g/g) and water absorption index (~5.7 g/g). The SSP system achieved the highest oil extraction yield, with no significant effect of pretreatment temperature. The oils extracted showed high levels of unsaturated fatty acids, particularly oleic acid (~48% of ω-9), highlighting their nutritional and industrial value. The findings support the valorisation of pistachio oil extraction by-products as functional food ingredients, offering a promising strategy for reducing food waste and promoting circular economy approaches in the agri-food sector. Full article
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23 pages, 2688 KiB  
Article
Effect of Biostimulant Applications on Eco-Physiological Traits, Yield, and Fruit Quality of Two Raspberry Cultivars
by Francesco Giovanelli, Cristian Silvestri and Valerio Cristofori
Horticulturae 2025, 11(8), 906; https://doi.org/10.3390/horticulturae11080906 (registering DOI) - 4 Aug 2025
Abstract
Enhancing the yield and qualitative traits of horticultural crops without further hampering the environment constitutes an urgent challenge that could be addressed by implementing innovative agronomic tools, such as plant biostimulants. This study investigated the effects of three commercial biostimulants—BIO1 (fulvic/humic acids), BIO2 [...] Read more.
Enhancing the yield and qualitative traits of horticultural crops without further hampering the environment constitutes an urgent challenge that could be addressed by implementing innovative agronomic tools, such as plant biostimulants. This study investigated the effects of three commercial biostimulants—BIO1 (fulvic/humic acids), BIO2 (leonardite-humic acids), and BIO3 (plant-based extracts)—on leaf ecophysiology, yield, and fruit quality in two raspberry cultivars, ‘Autumn Bliss’ (AB) and ‘Zeva’ (Z), grown in an open-field context, to assess their effectiveness in raspberry cultivation. Experimental activities involved two Research Years (RYs), namely, year 2023 (RY 1) and 2024 (RY 2). Leaf parameters such as chlorophyll, flavonols, anthocyanins, and the Nitrogen Balance Index (NBI) were predominantly influenced by the interaction between Treatment, Year and Cultivar factors, indicating context-dependent responses rather than direct biostimulant effects. BIO2 showed a tendency to increase yield (g plant−1) and berry number plant−1, particularly in RY 2 (417.50 g plant−1, +33.93% vs. control). Fruit quality responses were cultivar and time-specific: BIO3 improved soluble solid content in AB (12.8 °Brix, RY 2, Intermediate Harvest) and Z (11.43 °Brix, +13.91% vs. BIO2). BIO2 reduced titratable acidity in AB (3.12 g L−1) and increased pH in Z (3.02, RY 2) but also decreased °Brix in Z. These findings highlight the potential of biostimulants to modulate raspberry physiology and productivity but underscore the critical role of cultivar, environmental conditions, and specific biostimulant composition in determining the outcomes, which were found to critically depend on tailored application strategies. Full article
(This article belongs to the Section Fruit Production Systems)
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14 pages, 6826 KiB  
Article
Crack-Mitigating Strategy in Directed Energy Deposition of Refractory Complex Concentrated CrNbTiZr Alloy
by Jan Kout, Tomáš Krajňák, Pavel Salvetr, Pavel Podaný, Michal Brázda, Dalibor Preisler, Miloš Janeček, Petr Harcuba, Josef Stráský and Jan Džugan
Materials 2025, 18(15), 3653; https://doi.org/10.3390/ma18153653 - 4 Aug 2025
Abstract
The conventional manufacturing of refractory complex concentrated alloys (RCCAs) for high-temperature applications is complicated, particularly when material costs and high melting points of the materials processed are considered. Additive manufacturing (AM) could provide an effective alternative. However, the extreme temperatures involved represent significant [...] Read more.
The conventional manufacturing of refractory complex concentrated alloys (RCCAs) for high-temperature applications is complicated, particularly when material costs and high melting points of the materials processed are considered. Additive manufacturing (AM) could provide an effective alternative. However, the extreme temperatures involved represent significant challenges for manufacturing defect-free alloys using this approach. To address this issue, we investigated the preparation of a CrNbTiZr quaternary complex concentrated alloy from an equimolar blend of elemental powders using commercially available powder-blown L-DED technology. Initially, the alloys exhibited some defects owing to the internal stress caused by the temperature gradients. This was subsequently resolved by optimizing the deposition strategy. SEM, XRD and EDS were used to analyze the alloy in the as-deposited condition, revealing a BCC phase and a secondary Laves phase. Furthermore, Vickers hardness testing demonstrated a correlation between the hardness and the volume fraction of the Laves phase. Finally, successfully performed compression tests confirmed that the prepared material exhibits high-temperature strength and therefore is promising for high-temperature application under extreme conditions. Full article
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22 pages, 3515 KiB  
Article
Biodegradation of Chloroquine by a Fungus from Amazonian Soil, Penicillium guaibinense CBMAI 2758
by Patrícia de Almeida Nóbrega, Samuel Q. Lopes, Lucas S. Sá, Ryan da Silva Ramos, Fabrício H. e Holanda, Inana F. de Araújo, André Luiz M. Porto, Willian G. Birolli and Irlon M. Ferreira
J. Fungi 2025, 11(8), 579; https://doi.org/10.3390/jof11080579 - 4 Aug 2025
Abstract
Concern over the presence of pharmaceutical waste in the environment has prompted research into the management of emerging organic micropollutants (EOMs). In response, sustainable technologies have been applied as alternatives to reduce the effects of these contaminants. This study investigated the capacity of [...] Read more.
Concern over the presence of pharmaceutical waste in the environment has prompted research into the management of emerging organic micropollutants (EOMs). In response, sustainable technologies have been applied as alternatives to reduce the effects of these contaminants. This study investigated the capacity of filamentous fungi isolated from iron mine soil in the Amazon region to biodegrade the drug chloroquine diphosphate. An initial screening assessed the growth of four fungal strains on solid media containing chloroquine diphosphate: Trichoderma pseudoasperelloides CBMAI 2752, Penicillium rolfsii CBMAI 2753, Talaromyces verruculosus CBMAI 2754, and Penicillium sp. cf. guaibinense CBMAI 2758. Among them, Penicillium sp. cf. guaibinense CBMAI 2758 was selected for further testing in liquid media. A Box–Behnken factorial design was applied with three variables, pH (5, 7, and 9), incubation time (5, 10, and 15 days), and chloroquine diphosphate concentration (50, 75, and 100 mg·L−1), totaling 15 experiments. The samples were analyzed by gas chromatography–mass spectrometry (GC-MS). The most effective conditions for chloroquine biodegradation were pH 7, 100 mg·L−1 concentration, and 10 days of incubation. Four metabolites were identified: one resulting from N-deethylation M1 (N4-(7-chloroquinolin-4-yl)-N1-ethylpentane-1,4-diamine), two from carbon–carbon bond cleavage M2 (7-chloro-N-ethylquinolin-4-amine) and M3 (N1,N1-diethylpentane-1,4-diamine), and one from aromatic deamination M4 (N1-ethylbutane-1,4-diamine) by enzymatic reactions. The toxicity analysis showed that the products obtained from the biodegradation of chloroquine were less toxic than the commercial formulation of this compound. These findings highlight the biotechnological potential of Amazonian fungi for drug biodegradation and decontamination. Full article
(This article belongs to the Special Issue Fungal Biotechnology and Application 3.0)
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20 pages, 2272 KiB  
Article
An Important Step for the United States: Efforts to Establish the First Official Trade and Diplomatic Relations with the Ottoman Empire During the Process of Developing Its Economy
by Ebru Güher
Histories 2025, 5(3), 37; https://doi.org/10.3390/histories5030037 - 2 Aug 2025
Viewed by 223
Abstract
This study examines how the newly established United States pursued economic development through diplomatic and commercial initiatives with the Ottoman Empire, navigating regional powers and the era’s political-economic conditions. It analyzes using American archival sources how America endeavored to establish commercial and diplomatic [...] Read more.
This study examines how the newly established United States pursued economic development through diplomatic and commercial initiatives with the Ottoman Empire, navigating regional powers and the era’s political-economic conditions. It analyzes using American archival sources how America endeavored to establish commercial and diplomatic relations with the Ottoman Empire in the Mediterranean and Black Sea regions, which it viewed as critical markets in the late 18th and early 19th centuries, before signing any formal agreement. The research tracks how these early efforts laid foundations for what would become one of the world’s largest economies. The study analyzes America’s diplomatic efforts to secure an agreement with the Ottoman Empire prior to the 7 May 1830 trade agreement—which laid the foundation for bilateral relations—alongside the reactions of regional powers, the prevailing conditions of the period, and the Ottoman administration’s reluctance due to various factors, based on U.S. archival sources that, to the best of our knowledge, have not previously been utilized in existing studies. Full article
(This article belongs to the Section Political, Institutional, and Economy History)
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25 pages, 2786 KiB  
Article
Xylem Functional Anatomy of Pure-Species and Interspecific Hybrid Clones of Eucalyptus Differing in Drought Resistance
by José Gándara, Matías Nión, Silvia Ross, Jaime González-Tálice, Paolo Tabeira and María Elena Fernández
Forests 2025, 16(8), 1267; https://doi.org/10.3390/f16081267 - 2 Aug 2025
Viewed by 160
Abstract
Climate extremes threaten the resilience of Eucalyptus plantations, yet hybridization with drought-tolerant species may enhance stress tolerance. This study analyzed xylem anatomical and functional drought responses in commercial Eucalyptus grandis (GG) clones and hybrids: E. grandis × camaldulensis (GC), E. grandis × tereticornis [...] Read more.
Climate extremes threaten the resilience of Eucalyptus plantations, yet hybridization with drought-tolerant species may enhance stress tolerance. This study analyzed xylem anatomical and functional drought responses in commercial Eucalyptus grandis (GG) clones and hybrids: E. grandis × camaldulensis (GC), E. grandis × tereticornis (GT), and E. grandis × urophylla (GU1, GU2). We evaluated vessel traits (water transport), fibers (mechanical support), and wood density (D) in stems and branches. Theoretical stem hydraulic conductivity (kStheo), vessel lumen fraction (F), vessel composition (S), and associations with previous hydraulic and growth data were assessed. While general drought responses occurred, GC had the most distinct xylem profile. This may explain it having the highest performance in different irrigation conditions. Red gum hybrids (GC, GT) maintained kStheo under drought, with stable F and a narrower vessel size, especially in branches. Conversely, GG and GU2 reduced F and S; and stem kStheo declined for a similar F in these clones, indicating vascular reconfiguration aligning the stem with the branch xylem. Almost all clones increased D under drought in any organ, with the highest increase in red gum hybrids. These results reveal diverse anatomical adjustments to drought among clones, partially explaining their growth responses. Full article
(This article belongs to the Section Forest Ecophysiology and Biology)
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