Search Results (322)

The treatment of chronic wounds is one of the most complex therapeutic problems of modern medicine. It leads to patients’ protracted recovery, generating high treatment costs. Herbal products may be useful in the treatment of chronic wounds via a wide range of pharmacological properties and multidirectional effects on the wound healing phases. The study aims to determine the ability of selected plant extracts to modulate the processes involved in wound healing. The antimicrobial (MIC, MBC, MFC) and antioxidant (ABTS, DPPH) activities, cytotoxicity (MTT test), scratch wound test, and collagen assay were tested. R. canina (MBC 0.39 mg/mL) and V. venifera (MBC 3.13 mg/mL) extracts had bactericidal activities against P. aeruginosa and S. aureus, respectively. The V. vinifera extract showed the highest antioxidant activity in both ABTS (EC50 0.078 mg/mL) and DPPH (EC50 0.005 mg/mL) methods. The percentage of wound closure observed for C. cardunculus, R. rosea, and R. canina extracts with HaCaT, and V. vinifera extract with Hs27 cells was set as 100%. V. vinifera extract (50 μg/mL) stimulated collagen synthesis 5.16 times more strongly than ascorbic acid. Our preliminary study showed that some plant extracts may be promising modulators of the wound healing process, although further in-depth studies are necessary to determine their effectiveness in the in vivo model. Full article

Background: Studies have suggested a synergism between lenalidomide (LEN) and ibrutinib (IBR) in multiple myeloma (MM). Both downregulate IRF4, a key target and master transcriptional factor regulating myeloma cell survival. Method: A 3 + 3 phase I trial was conducted to determine the maximum tolerated dose (MTD) of IBR in combination with LEN + dexamethasone (DEX) in patients with relapsed/refractory (RR) MM who had at least one prior line of therapy. Three dose levels (DLs) were planned. The cycle length was 28 days. IBR was administered orally daily in doses of 560 mg on DL1-2 and 840 mg on DL3, LEN was administered orally on days 1–21 in doses of 15 mg on DL1 and 25 mg on DL2-3, and DEX was administered orally on days 1, 8, 15, and 22 in a dose of 40 mg if age < 75 years or in a dose of 20 mg if it was ≥75 years for DL1-3. Patients with a glomerular filtration rate (GFR) <60 but ≥30 mL/min were treated in accordance with the manufacturer’s instructions with LEN 10 mg. Dose-limiting toxicities (DLTs) included the following: grade 4 neutropenia lasting more than 5 days, thrombocytopenia, febrile neutropenia, nausea, vomiting or diarrhea; grade 3 thrombocytopenia with bleeding or platelet transfusion; and grade 3–4 hyperglycemia or a thrombotic/embolic event, and other nonhematologic toxicities. The overall response rate (ORR) was defined as the percentage of patients with a partial response (PR), very good partial response (VGPR), or complete response (CR) according to IMWG criteria on two consecutive evaluations at least 4 weeks apart. The clinical benefit rate (CBR) was defined as the percentage of patients with stable disease (SD) or a better outcome on two consecutive evaluations at weeks apart. Results: Fourteen patients (DL1: six patients; DL2: three patients; DL3: five patients) were registered for the study from March 2019 to May 2023, prior to its closure due to limited accrual. Thirteen patients are included in the summary of toxicities and response as one patient on DL3 halted participation prior to the start of the treatment. Two patients on DL3 were excluded from the determination of MTD: one having discontinued cycle 1 treatment due to COVID-19 infection and the another having mistakenly taken 280 mg/day of IBR instead of the assigned 840 mg/day dose during cycle 1. Only one patient developed a DLT, on DL1 with grade 3 non-viral hepatitis. The median number of cycles administered was 4 (range: 1–56). Severe toxicities reported included grade 4 lymphocytopenia (1), grade 4 thrombocytopenia (1), and grade 5 sepsis in the setting of PD (1). Disease responses included a VGPR on DL1 and CR on DL3. Thus, the ORR was 15.4% (90% CI: 2.8–41.0%). One patient on DL1 maintained SD for 4.6 years before discontinuing the treatment to undergo an alternative therapy. Another five patients maintained SD for ≥ 2 consecutive cycles. Thus, the CBR was 61.5% (90% CI: 35.5–83.4%). Conclusions: The combination of LEN with IBR in RR MM proved feasible, with manageable toxicities and the majority of discontinuations being due to disease progression. Full article

Humulus lupulus L. (hop) is a multipurpose crop valued for its essential role in beer production and for its bioactive compounds with recognized medicinal properties. Otherwise, climate change represents a major challenge to agriculture, particularly impacting the cultivation of crops with stenoecious characteristics, such as hop. This highlights the urgent need to enhance crop resilience to adverse environmental conditions. The phytohormone abscisic acid (ABA) is a key regulator of plant responses to abiotic stress, yet the ABA signaling pathway remains poorly characterized in hop. Harnessing the publicly available hop genomics resources, we identified eight members of the PYRABACTIN RESISTANCE 1 LIKE ABA receptor family (HlPYLs). Phylogenetic and gene structure analyses classified these HlPYLs into the three canonical ABA receptor subfamilies. Furthermore, all eight HlPYLs are likely functional, as suggested by the protein sequence visual analysis. Expression profiling indicates that ABA perception in hop is primarily mediated by the HlPYL1-like and HlPYL8-like subfamilies, while the HlPYL4-like group appears to play a more limited role. Structure modeling and topology predictions of HlPYL1b and HlPYL2 provided insights into their potential functional mechanisms. To assess the physiological relevance of ABA signaling in hop, we evaluated the impact of exogenous ABA application during the ex vitro acclimatization phase. ABA-treated plants exhibited more robust growth, reduced stress symptoms, and improved acclimatization success. These effects were associated with reduced leaf transpiration and enhanced stomatal closure, consistent with ABA-mediated drought tolerance mechanisms. Altogether, this study provides the first comprehensive characterization of ABA receptor components in hop and demonstrates the practical utility of ABA in improving plant performance under ex vitro conditions. These findings lay the groundwork for further functional studies and highlight ABA signaling as a promising target for enhancing stress resilience in hop, with broader implications for sustainable agriculture in the face of climate change. Full article

Background: Surface electromyography (sEMG) enables the non-invasive assessment of muscle activity and is widely used in orthodontics for evaluating masticatory muscles. However, little is known about the dynamic changes in facial expression muscles during orthodontic treatment. This study aimed to investigate alterations in facial muscle tone during the leveling and alignment phase in adult female patients undergoing fixed appliance therapy. Methods: The study included 30 female patients aged 20–31 years who underwent sEMG assessment at four time points: before treatment initiation (T0), at the start of appliance placement (T1), three months into treatment (T2), and six months into treatment (T3). Muscle activity was recorded during four standardized facial expressions: eye closure, nasal strain, broad smile, and lip protrusion. Electrodes were placed on the orbicularis oris, orbicularis oculi, zygomaticus major, and levator labii superioris alaeque nasi muscles. A total of 1440 measurements were analyzed using Friedman and Conover-Inman tests (α = 0.05). Results: Significant changes in muscle tone were observed during treatment. During lip protrusion, the orbicularis oris and zygomaticus major showed significant increases in peak and minimum activity (p < 0.01). Eye closure was associated with altered orbicularis oris activation bilaterally at T3 (p < 0.01). Nasal strain induced significant changes in zygomaticus and levator labii muscle tone, particularly on the right side (p < 0.05). No significant changes were noted during broad smiling. Conclusions: Orthodontic leveling and alignment influence the activity of selected facial expression muscles, demonstrating a dynamic neuromuscular adaptation during treatment. These findings highlight the importance of considering soft tissue responses in orthodontic biomechanics and suggest potential implications for facial esthetics and muscle function monitoring. Full article

This article addresses the impact of deficit irrigation on the agronomic and physiological performance of “Rojo Brillante” persimmon trees in a Mediterranean climate. It compares the effect of a sustained deficit irrigation (SDI; imposing water deficit uniformly throughout the entire crop cycle) strategy and two regulated deficit irrigation (RDI; enforcing a water deficit during the phenological phases that are less sensitive to water stress) strategies. Field trials were conducted from 2022 to 2024 at the Cajamar Experimental Center in Paiporta, Valencia, Spain. The trees respond to mild water stress reducing transpiration through stomatal closure. RDI resulted in modest irrigation water savings (11–16%), minimizing fruit drop, leading to an increased number of fruits per tree and a higher marketable yield, although this came at the cost of a reduced unit fruit weight. SDI achieved a 30% reduction in irrigation water usage without impacting on the marketable yield, but it also caused a decrease in unit fruit weight. RDI increased water productivity (yield obtained per amount of water applied) primarily through higher yields, while SDI improved productivity mainly by lowering the amount of irrigation water applied. Both irrigation strategies are recommended for cultivating “Rojo Brillante” persimmons. RDI is especially advisable in years with lower fruit loads as more intensive thinning may be necessary in years with higher fruit loads. Conversely, SDI is recommended in situations where water availability is limited. Full article

Aeroponics efficiently conserves water and fertilizer but faces energy sustainability challenges in maintaining high productivity and quality. This study aimed to identify critical growth phases of lettuce affected by management modes and assess resource/energy efficiency (cost per unit yield) to inform the development of sustainability strategies for lettuce production in a lettuce-dominant aeroponics system integrated with radish. Three management modes were tested: M1 (constant nutrient solution concentrations), M2 (variable nutrient solution concentrations), and M3 (combined variable nutrient solution concentrations and preharvest short-duration continuous light for 48 h). Plant parameters were dynamically measured in a 30-day cultivation cycle. The results showed that the intercropped lettuce exhibited peak growth at 15–25 days after transplanting, and nutrient solution adjustment enhanced the shoot weight and quality, with synergistic quality improvements under M3. However, preharvest lighting reduced the net photosynthetic rate via stomatal closure and lowered the effective quantum yield of photosystem II, preventing biomass increase. The preharvest short-duration continuous light elevated the soluble protein, ascorbic acid, and soluble sugar contents. For yield-focused systems, M2 alone achieved comparable shoot weight to M3 with higher energy efficiency. However, when simultaneously considering lettuce quality enhancement and the yield boost of radish in the intercropping system, M3 demonstrated potential for greater marginal benefits. Full article

Background and Objectives: Patients with unilateral cleft lip and palate (UCLP) require a phase of infant orthopedic treatment prior to surgical cleft closure. Treatment planning in this phase necessitates a thorough understanding of maxillary growth dynamics in this period. The aim of the present study was to evaluate the quantitative and qualitative surface growth of maxillary segments in infants with UCLP. Materials and Methods: In total, 195 intraoral scans from 50 patients were obtained postnatal (T0), at monthly intervals (T1–5), and prior to surgical cleft closure at 6 months of age (T6). Surface, linear, and angle measurements of the maxillary segments were performed. Results: Significant increases in the total surface area and the surface areas of the small and large segments were observed at monthly intervals and over the overall duration. The large segment showed greater absolute growth (11.62 mm² per month, 46.57 mm² total), while the small segment had a higher percentage increase (1.49% monthly, 6.57% overall). A positive small correlation was observed between surface area growth changes in the small segment and its increase in length. Conclusions: Our results revealed distinct growth patterns of the large and small segments in amount and direction, underscoring the relevance of incorporating segment arch width in surface evaluations. Full article

Sparkling wine is a complex alcoholic beverage with high economic value, produced through a secondary fermentation of a still wine, followed by a prolonged aging period that may last from nine months to several years. With the growing global demand for high-quality sparkling wines, understanding the biochemical mechanisms related to aroma development has become increasingly relevant. This review provides a comprehensive overview of the secondary fermentation process, with particular emphasis on yeast selection, types of closure, and the impact of aging on the volatile composition. Special attention is also given to the analytical strategies employed for the identification and quantification of target compounds in sparkling wine matrices. Due to the presence of volatile compounds at trace levels, effective extraction and pre-concentration techniques are essential. Extraction methods such as solid-phase microextraction (SPME), stir-bar sorptive extraction (SBSE), and thin-film SPME (TF-SPME) are discussed, as well as chromatographic techniques, such as gas chromatography (GC) and liquid chromatography (LC). Full article

Industrial and urban activity has inevitably changed the water environment and caused significant impacts on water resources’ quality and quantity. The identification of related impacts is particularly important in the context of increasing water shortages due to climate change. Overlapping industrial impacts and drought occurrence have resulted in the long-lasting deterioration of surface water status. Therefore, the mitigation of negative impacts is crucial for relevant and sustainable water management in river basins. One of the most impactful branches of industry is underground coal mining, which requires dewatering deposits and excavations. Mine waters discharged into rivers have induced significant increases of salinity, while urban wastewaters have increased biogenic contamination in surface waters. Sustainable development goals require water protection, energy transition, and circularity; therefore, coal will be repurposed in favor of alternative sources of energy. The phasing out of coal and cessation of dewatering of mines would rapidly reduce mine waters’ impact on the environment. However, in heavily industrialized urban basins, the share of natural waters in river flows is exceptionally low—due to significant and long-lasting transformations, industrial and urban wastewaters are the main constitutive components in certain river hydrological regimes. The case study of Bytomka in the Upper Silesian Coal Basin, Southern Poland is a vivid example of a river basin significantly impacted by urban and industrial activity over a long-term period. The Bytomka River’s water status and the development of its watershed area is an example of complex and overlapping impacts, wherein sustainable water management requires proper recognition of prevailing factors such as mine water discharges, climate change and drought periods, wastewater impacts, and urbanization of the water basin area. The presented study reveals key findings showing that future coal mine closures would result in significant water resource shortages due to a reduction of mine water discharges, significant biogenic (N and P) pollution increases, and hazards of harmful algal blooms. Therefore, there is an urgent need to increase the retention potential of the watershed, use nature-based solutions, and mitigate negative impacts of the coal mining transition. The increase in treatment capability of industrial wastewater and sewage discharge would help to cope with the natural water vulnerability induced by the impacts of climate change. Full article

Background: Diabetic foot ulcers present a significant clinical challenge because of their high prevalence and severe complications. The need for innovative and accessible treatment options is critical. Owing to their medicinal properties, natural products, such as geopropolis, hold promise. However, the wound healing potential of the geopropolis of Melipona fasciculata, particularly in accelerating the healing of diabetic ulcers, remains unexplored. In this study, we evaluated the ability of the geopropolis of M. fasciculata to promote wound healing in diabetic mice. Methods: Geopropolis was collected, prepared as a hydroalcoholic extract, and formulated into a topical cream. Non-obese diabetic (NOD) mice with induced chronic wounds were treated with this cream daily, and wound healing was assessed through macroscopic measurements, histological analysis, cytokine quantification, and in silico molecular docking studies. Results: The results demonstrated that, compared with the control treatment, the geopropolis cream accelerated wound closure at all the analyzed time points (days 3, 7, and 14), reduced inflammatory infiltrates, and enhanced fibroblast proliferation and collagen deposition. These alterations were particularly pronounced in the final phase of healing, indicating an improvement in wound repair processes. These effects occurred without altering systemic cytokine levels, suggesting a localized treatment action. These results may be partially associated with the theoretical ability of beta-amyrin and cycloartenol to interact with human myeloperoxidase (MPO), as suggested by in silico docking analysis. Conclusions: Overall, the findings indicate that geopropolis cream could represent a viable alternative for managing diabetic ulcers, providing an effective means to enhance wound healing while remaining accessible to low-income populations. Full article

Carbon Capture and Storage (CCS) is a critical strategy for reducing CO₂ emissions from hard-to-abate sectors. Reliable and efficient reservoir simulation tools are essential for supporting the safe and effective deployment of CCS projects. This study presents a twofold contribution to CCS modeling in saline aquifers: (1) the validation of the Black Oil Model (BoM) as a computationally efficient alternative to compositional simulators, and (2) a systematic assessment of the impact of grid resolution on plume prediction accuracy. The BoM was benchmarked against three commercial compositional simulators—Eclipse E300, CMG-GEM, and TNavigator. The comparison focused on key aspects of CO₂ storage operations, including plume evolution to assess containment and storage security, as well as injection safety and efficiency through pressure and saturation profile analysis, evaluated across both the injection and the post-closure monitoring phases. The BoM successfully reproduced plume extent and CO₂ saturation distributions, with mean deviations of 3% during injection, 5% during post-closure, and an overall average of 4% across the entire project duration. Additionally, simulation times were reduced by a factor of four compared to compositional models. These results confirm the BoM’s practical utility as a robust and efficient tool for CO₂ storage simulation. In parallel, the study investigated the influence of vertical and lateral grid resolutions/coarsening on the accuracy of CO₂ modeling. Seven models were developed and evaluated using a hybrid qualitative–quantitative framework, consistent with the BoM validation methodology. Vertical resolution was found to be particularly critical during the monitoring phase. While a 5 m resolution proved adequate during injection, deviations in plume shape and magnitude during post-injection increased to an average of 15% compared to a fine 2 m vertical resolution model, highlighting the necessity of fine vertical discretization (≤2 m) to capture gravity-driven plume dynamics during the monitoring phase. Conversely, lateral grid resolution had a stronger effect during the injection phase. A lateral cell size of 150 m was required for accurate plume prediction, with 200 m remaining moderately acceptable for early-phase assessment and prospect ranking, whereas coarser lateral grids led to significant underestimation of plume spread and dissolution extent. These findings demonstrate that the BoM, when combined with informed grid resolution strategies, enables accurate and computationally efficient simulation of CO₂ storage in saline aquifers. The study provides practical guidelines for fluid model selection and spatial discretization, offering critical input to subsurface experts involved in CCS project development, monitoring design, and regulatory compliance. Full article

Skin health must be ensured at all times in the case of wounds when the skin is subjected to traumatic actions that require multiple wound-healing measures. Wound healing is a complex, multi-phase biological process critical for restoring skin integrity after trauma. This study investigates the development and evaluation of a novel composite hydrogel formulated from collagen peptides extracted from the jellyfish Rhizostoma pulmo and hydroethanolic extracts from the brown alga Cystoseira barbata, both sourced from the Romanian Black Sea coast. Throughout the work, the characteristics due to the biochemical compositions of the extracts from the brown alga C. barbata and from the jellyfish R. pulmo are highlighted as important, emphasizing the content of polysaccharides, proteins, and lipids. Total phenol content was analyzed for three extracts from natural products. The biochemical composition, antioxidant, antimicrobial, and in vitro wound-healing properties of the components and their composite (JPC-ALG) were assessed. The rheological behavior and optical microscopy studies of collagen hydrogels were prepared. The general mechanisms of wound healing with the involvement of polysaccharides and collagen peptides existing in all categories of extracts were highlighted. The study of the effects of JPC-ALG composites and individual extracts on fibroblast and keratocyte cell lines is also presented. Results demonstrated that the composite exhibited synergistic effects, enhancing fibroblast and keratinocyte migration and proliferation, key factors in wound closure. The findings support the potential application of this marine-derived bioactive composite as a promising biomaterial for wound-healing therapies. Full article

Topological photonics has provided revolutionary ideas for the design of next-generation photonic devices due to its unique light transmission properties. This paper proposes a honeycomb photonic crystal structure based on a mirror-symmetric interface and numerically simulates the precise manipulation of topological edge states and the robust excitation of high-order topological corner states in this structure. Specifically, two honeycomb photonic crystals with non-trivial topological properties form an interface through mirror-symmetric stitching. Continuous adjustment of the spacing between their coupling pillars can induce the closure and reopening of topological edge state energy bands, accompanied by significant band inversion, revealing the dynamic process of topological phase transitions. Furthermore, zero-dimensional high-order topological corner states are observed at the junction of boundaries with different topological properties. Their localized field strengths are strictly confined and exhibit strong robustness against structural defects. This study not only provides a new mechanism for the local symmetry manipulation of topological edge states but also lays a foundation for the design of high-order topological photonic crystals and the practical application of topological photonic devices. Full article

This paper considers the spatial distribution of gold occurrences, their geochemical anomalies, and late Mesozoic igneous complexes within the framing of the eastern flank of the Mongol–Okhotsk orogenic belt (EF MOOB). It is established that elevated gold concentrations are associated with telescoped igneous complexes formed in different geodynamic regimes. The southern framing of the EF MOOB (Russia) was chosen as the key study area due to its well-preserved superposition of multi-stage igneous events. These stages are considered using the example of the Uskalin intrusive massif. It is a representative example where three geodynamic phases are recorded, namely initial supra-subduction (149–138 Ma), subduction (140–122 Ma), and collision (119–97 Ma). It is shown that the massif is composed of granitoids aged 145 Ma, 129 Ma, and 112 Ma, which correspond to the distinguished geodynamic stages. Geochemical characteristics of the rocks of the first two stages completely coincide with those of the rocks corresponding to the geodynamic stages. The exception is the formations from the collision process. At this stage, differences appear in the rocks, which are manifested in the Sr/Y ratio. These values are comparable with those in the granitoids of the adakite series. Such differences were established only within gold-bearing areas. The formation of the Uskalin massif was accompanied by extensive mineralization zones with gold-bearing veins. Gold concentrations in granitoids of the adakite series (145 Ma) exceed the crustal Clarke value by 2.25 times, which directly links mineralization with magmatic processes. It is assumed that the presence of collision-stage rocks with signs of the adakite signature may be one of the signs of detection of epithermal gold ore objects in the zones of magmatic telescoping. Taking into account the evolution of the MOOB associated with the closure of the MOB and with the accompanying magmatic events, an analog of which is considered using the example of the southern framing of the EF MOOB, it is possible to assume the use of the obtained results in conducting exploration work for ore gold in this region. Full article

This study investigates damage characteristics of red sandstone under dynamic loads to clarify the effects of construction disturbances and blasting on the stability of surrounding rock during mountain tunnel construction in water-rich strata. Dynamic impact experiments at various loads were conducted using the Split Hopkinson Pressure Bar (SHPB) instrument, complemented by simulations of the fracturing process in saturated sandstone using finite element software. This analysis systematically examines the post-fracture granularity mass fraction, stress-strain curves, peak stress-average strain rate relationship, and fracture patterns. The dynamic response mechanism of red sandstone during the process of tunnel blasting construction was thoroughly investigated. Experimental results reveal that the peak stress and failure strain exhibit strain rate dependency, increasing from 45.65 MPa to 115.34 MPa and 0.95% to 5.23%, respectively, as strain rate elevates from 35.53 s⁻¹ to 118.71 s⁻¹. The failure process of red sandstone is divided into four stages: crack closure, nearly elastic phase, rapid crack development, and rapid unloading. Dynamic peak stress and average strain rate in sandstone demonstrate an approximately linear relationship, with the correlation coefficient being 0.962. Under different impact loads, fractures in specimens typically expand from the edges to the center and evolve from internal squeezing fractures to external development. Peak stress, degree of specimen breakage, and energy dissipation during fracturing are significantly influenced by the strain rate. The numerical simulations confirmed experimental findings while elucidating the failure mechanism in surrounding rocks under varying strain rates. This work pioneers a multiscale analysis framework bridging numerical simulation with a blasting construction site, addressing the critical gap in time-dependent deformation during tunnel excavation. Full article