Search Results (554)

The genus Curcuma (Zingiberaceae) is a diverse group of plants widely distributed across tropical Asia, with several new species recently described in Thailand. This study documents and clarifies the taxonomic status of a new species, Curcuma nivea Saensouk, P.Saensouk & Boonma sp. nov., discovered in eastern Thailand, and evaluates its horticultural potential. Morphological comparisons were conducted with closely related species in the Curcuma subgenus Hitcheniopsis (Baker) K. Schum., focusing on diagnostic vegetative and floral traits. Curcuma nivea is characterized by its compact habit and white flowers marked with two reddish lines at the base of the labellum, lacking the yellow blotch typical of related species. Additionally, it shows the absence of both epigynous glands and anther spurs, consistent with subgeneric features. Its distinctive morphology and attractive floral display have led to its cultivation as an ornamental pot plant. The discovery of C. nivea contributes to the growing documentation of Curcuma diversity in Thailand and underscores the significance of ongoing botanical exploration and conservation. Furthermore, its compact form and unique floral traits highlight its promise for use in ornamental horticulture and breeding programs. Full article

In the process of the work of a coal power station is formed ash and slag, which, along with process water, are deposited in the dumps. Coal ash waste dumps significantly degrade the surrounding environment due to their unprotected surfaces, which are highly susceptible to wind and water erosion. This results in the dispersion of contaminants into adjacent ecosystems. Pollutants migrate into terrestrial and aquatic systems, compromising soil quality and water resources, and posing documented risks to the environment and human health. Primary succession on the coal ash dumps of the Apatity thermal power plant (Murmansk Region, NW Russia) was initiated by cyanobacterial colonization. We studied cyanobacterial communities inhabiting three spoil sites that varied in time since decommissioning. These sites are characterized by exceptionally high concentrations of calcium and magnesium oxides—levels approximately double those found in the region’s natural soils. A total of 18 cyanobacterial taxa were identified in disposal sites. Morphological analysis of visible surface crusts revealed 16 distinct species. Furthermore, 24 cyanobacterial strains representing 11 species were successfully isolated into unialgal culture and tested with a molecular genetic approach to confirm their identification from 16S rRNA. Three species were determined with molecular evidence. Cyanobacterial colonization of coal fly ash disposal sites begins immediately after deposition. Primary communities initially exhibit low species diversity (four taxa) and do not form a continuous ground cover in the early years. However, as succession progresses—illustrated by observations from a 30-year-old deposit—spontaneous surface revegetation occurs, accompanied by a marked increase in cyanobacterial diversity, reaching 12 species. Full article

This study investigates the genetic diversity and population structure of Castanopsis tribuloides, a vital tree species in Asian forest ecosystems. Understanding the genetic patterns of keystone forest species provides critical insights into forest resilience and ecosystem function and informs conservation strategies. We analyzed population samples collected from three distinct locations within Doi Suthep Mountain in northern Thailand using Short Tandem Repeat (STR) markers to assess both intra- and inter-population genetic relationships. DNA was extracted from leaf samples and analyzed using a panel of polymorphic microsatellite loci specifically optimized for Castanopsis species. Statistical analyses included the assessment of forensic parameters (number of alleles, observed and expected heterozygosity, gene diversity, polymorphic information content), population differentiation metrics (G_ST), inbreeding coefficients (F_IS), and gene flow estimates (N_m). We further examined population history through bottleneck analysis using three models (IAM, SMM, and TPM) and visualized genetic relationships through principal coordinate analysis and cluster analysis. Our results revealed significant patterns of genetic structuring across the sampled populations, with genetic distance metrics showing statistically significant differentiation between certain population pairs. The PCA and cluster analyses confirmed distinct population groupings that correspond to geographic distribution patterns. These findings provide the first comprehensive assessment of C. tribuloides population genetics in this region, establishing baseline data for monitoring genetic diversity and informing conservation strategies. This research contributes to our understanding of how landscape features and ecological factors shape genetic diversity patterns in essential forest tree species, with implications for managing forest genetic resources in the face of environmental change. Full article

The review summarizes the historical and current research on perennial grain breeding in Russia within the context of growing global interest in perennial crops. N.V. Tsitsin’s pioneering work in the 1930s produced the first wheat–wheatgrass amphiploids, which demonstrated the capacity to regrow after harvest and survive for 2–3 years. Subsequent research at the Main Botanical Garden in Moscow focused on characterizing Tsitsin’s material, selecting superior germplasm, and expanding genetic diversity through new cycles of hybridization and selection. This work led to the development of a new crop species, Trititrigia, and the release of cultivar ‘Pamyati Lyubimovoy’ in 2020, designed for dual-purpose production of high-quality grain and green biomass. Intermediate wheatgrass (Thinopyrum intermedium) is native to Russia, where several forage cultivars have been released and cultivated. Two large-grain cultivars (Sova and Filin) were developed from populations provided by the Land Institute and are now grown by farmers. Perennial rye was developed through interspecific crosses between Secale cereale and S. montanum, demonstrating persistence for 2–3 years with high biomass production and grain yields of 1.5–2.0 t/ha. Hybridization between Sorghum bicolor and S. halepense resulted in two released cultivars of perennial sorghum used primarily for forage production under arid conditions. Russia’s agroclimatic diversity in agricultural production systems provides significant opportunities for perennial crop development. The broader scientific and practical implications of perennial crops in Russia extend to climate-resilient, sustainable agriculture and international cooperation in this emerging field. Full article

The spatial distribution of ephemeral and perennial dryland plant species is increasingly modified and restricted by ever-changing climates and development expansion. At the interface of biodiversity conservation and developmental planning in desert landscapes is the growing need for adaptable tools in identifying and monitoring these ecologically fragile plant assemblages, habitats, and, often, heritage sites. This study evaluates usage of Sentinel-2 time series composite imagery to discriminate vegetation assemblages in a hyper-arid landscape. Spatial predictor spaces were compared to classify different vegetation communities: spectral components (PCs), vegetation indices (VIs), and their combination. Further, the uncertainty in discriminating field-verified vegetation assemblages is assessed using Shannon entropy and intensity analysis. Lastly, the intensity analysis helped to decipher and quantify class transitions between maps from different spatial predictors. We mapped plant assemblages in 2022 from combined PCs and VIs at an overall accuracy of 82.71% (95% CI: 81.08, 84.28). A high overall accuracy did not directly translate to high class prediction probabilities. Prediction by spectral components, with comparably lower accuracy (80.32, 95% CI: 78.60, 81.96), showed lower class uncertainty. Class disagreement or transition between classification models was mainly contributed by class exchange (a component of spatial allocation) and less so from quantity disagreement. Different artefacts of vegetation classes are associated with the predictor space—spectral components versus vegetation indices. This study contributes insights into using feature extraction (VIs) versus feature selection (PCs) for pixel-based classification of plant assemblages. Emphasising the ecologically sensitive vegetation in desert landscapes, the study contributes uncertainty considerations in translating optical satellite imagery to vegetation maps of arid landscapes. These are perceived to inform and support vegetation map creation and interpretation for operational management and conservation of plant biodiversity and habitats in such landscapes. Full article

Botanical gardens serve as vital centers for ex situ conservation, maintaining diverse plant species under controlled conditions. Terrestrial orchids, despite their wide diversity and distribution, often occur in small and declining populations, making their conservation increasingly urgent. This study aimed to examine the potential for establishing the first specialized orchidarium in Serbia, focusing on the native orchid species of the Fruška Gora region. A SWOT analysis, combined with site assessment data, was employed to identify key strengths, weaknesses, opportunities, and threats, informing the development of a functional zoning plan. The results indicate that such an orchidarium would offer a threefold benefit: strengthening ex situ conservation, advancing scientific research and environmental education, and promoting sustainable tourism. The proposed design consists of eight distinct zones, three of which reflect natural habitats of selected orchid species. The planned integration of a seed gene bank in the central zone, along with living plant collections and a nearby in vitro culture laboratory, establishes a comprehensive framework for the sustainable management of orchid genetic resources in the region, forming a foundation for future research and preservation. Full article

The Bignoniaceae family encompasses numerous species of ecological, medicinal, and cultural significance, yet its ethnobotanical value remains underexplored in many regions of Thailand. This study investigates the diversity, phenology, cultural relevance, and traditional uses of Bignoniaceae species in Maha Sarakham Province, Northeastern Thailand. Through semi-structured interviews with 260 local informants across 13 districts—alongside field observations and herbarium voucher collections—we documented 27 species across 21 genera. These integrated methods enabled the identification of key culturally significant species and provided insights into their traditional uses. Phenological data revealed clear seasonal patterns in flowering and fruiting, aligned with the regional climatic cycle. Quantitative ethnobotanical indices—including Species Use Value (SUV), Genera Use Value (GUV), Relative Frequency of Citation (RFC), Cultural Importance Index (CI), and Cultural Food Significance Index (CFSI)—were employed to evaluate species significance. Results indicate that species such as Dolichandrone serrulata, D. spathacea, and Oroxylum indicum hold high cultural and practical value, particularly in traditional medicine, spiritual practices, and local landscaping. These findings underscore the critical role of Bignoniaceae in sustaining biocultural diversity and emphasize the urgency of preserving traditional botanical knowledge amid environmental and socio-economic change. Moreover, the insights contribute to broader efforts in cultural heritage preservation and biodiversity conservation across tropical and subtropical regions. Full article

The temperate rainforests and their associated coastline in Scotland have long been home to dye-producing plants including lichens, some commercialised and patented by George and Cuthbert Gordon in 1758 as the dye Cudbear. Using merchant records and family correspondence, this paper will shed new light on the early trade and manufacture of Cudbear and also Cuthbert Gordon’s later investigations into other colour-producing plants undertaken as chemistry and botany emerged as fields of economic opportunity and study in the 18th century. It appears that Cudbear was used for longer and more widely than has previously been supposed and was integrated into known dye practices and used later with synthetic dyes. A rare pattern book of dyed wool flannel samples, held at the Royal Botanic Garden of Edinburgh, shows a re-evaluation of indigenous dye plants, especially lichens, in a time of national emergency in 1916. The result of a government initiative during WW1 and produced under laboratory conditions at the University of Glasgow, it is used here to identify some of the colours studied by Cuthbert Gordon of which we have little contemporary record. Full article

Bulgarian botanical gardens harbor more than 3600 plant species from across the world. Some of them are well-known plants widely used by humans, others are underutilized crops or little-known exotic species. The latter group constitutes a rich reservoir of plant resources whose potential to bring benefits to society is still untapped. The aim of this review is to describe the diversity of species and their potentially valuable secondary metabolites in three of the largest Bulgarian botanical gardens, with a focus on underutilized crops and medicinal plants that are typical of Bulgaria. With this, we aim to pave the way for future research on the most promising of these plants. The report includes currently available ethnobotanical data on the properties and composition of their bioactive components, known culinary or therapeutic uses, and nutritional profiles. We also outline the vast potential of these plants in providing healthy diets, as well as for performing future groundbreaking biomedical research. Finally, we present the approach that will be used to screen extracts from these plants for biological activity. Full article

The current study investigates the chemical profiling, antioxidant activities, and enzyme inhibitory and cytotoxic potential of the water and methanolic extracts of different parts (flower, leaf, and bulb) of Muscari armeniacum. Chemical profiling was performed using UHPLC-MS/MS. At the same time, different in vitro assays were employed to support the results for antioxidant potential, such as DPPH, ABTS, FRAP, CUPRAC, metal chelation, and PBD, along with the measurement of total phenolic and flavonoid contents. Enzyme inhibition was investigated for cholinesterase (AChE and BChE), α-amylase, α-glucosidase, and tyrosinase enzymes. Additionally, the relative expression of NRF2, HMOX1, and YGS was evaluated by qPCR. LC-MS/MS analysis indicated the presence of some significant compounds, including apigenin, muscaroside, hyacinthacine A, B, and C, and luteolin. According to the results, the highest TPC and TFC were obtained with both extracts of the leaves, followed by the water extract (flower) and methanolic extract of the bulb. In contrast, the methanolic extract from the bulb exhibited the highest antioxidant potential using DPPH, ABTS, CUPRAC, and FRAP, followed by the extracts of leaves. In contrast, the leaf extracts had the highest values for the PBD assay and maximum chelation ability compared to other tested extracts. According to the enzyme inhibition studies, the methanolic extract from the bulb appeared to be the most potent inhibitor for all the tested enzymes, with the highest values obtained for AChE (1.96 ± 0.05), BChE (2.19 ± 0.33), α-amylase (0.56 ± 0.02), α-glucosidase (2.32 ± 0.01), and tyrosinase (57.19 ± 0.87). Interestingly, the water extract from the bulb did not inhibit most of the tested enzymes. The relative expression of NRF2 based on qPCR analysis was considerably greater in the flower methanol extract compared to the other extracts (p < 0.05). The relative expression of HMOX1 was stable in all the extracts, whereas YGS expression remained stable in all the treatments and had no statistical differences. The current results indicate that the components of M. armeniacum (leaves, flowers, and bulb) may be a useful source of natural bioactive compounds that are effective against oxidative stress-related conditions, including hyperglycemia, skin disorders, and neurodegenerative diseases. Complementary in silico approaches, including molecular docking, dynamics simulations, and transcription factor (TF) network analysis for NFE2L2, supported the experimental findings and suggested possible multi-target interactions for the selected compounds. Full article

In this work, we evaluated the silvicultural and ecological parameters of Populus bolleana Lauche trees growing in conditions of anthropogenic pollution, using the example of one of the largest megacities of the Donetsk ridge, the city of Donetsk. The objectives of this study included determining the level of anthropogenic load of the territory; conducting dendrological studies to assess morphometric and allometric parameters, age structure, and condition of P. bolleana stands under the influence of environmental factors; as well as completing biomechanical studies to assess and predict the mechanical stability of stands. A total of 1109 plants growing in areas with increased anthropogenic load and in the control areas were studied. The model territories of the study were located in the city of Donetsk on Fallen Communards Avenue (length of field routes: 2.6 km) and Ilyicha Avenue (length of field routes: 9.7 km). Control plantings grew on the territory of the Donetsk botanical garden and residential (dormitory) districts of the city. The age structure of P. bolleana plantations remained uniform throughout the city for 50–55 years due to the fact that the landscaping was under a single state program. In the steppe zone in the south of the East European Plain, with a high level of anthropogenic load and severe natural climatic factors, the critical age of P. bolleana (55 years) was determined. The condition of plantations and their morphometric indices correlate with the level of anthropogenic load of the city (H, Dbase, DBH). Under control conditions, the plants are in good condition with signs of weakening (2 points). Under conditions of increased anthropogenic load, the plants are in a severely weakened condition (3 points). A total of 25% of the plants in the sample are in critical condition (4–5 points). The main damages to the crowns and trunks of plants include core rot, mechanical damage to bark and tissues, the development of core rot through the affected skeletal branch, crown thinning, and drying. P. bolleana trees are valued for their crown area and ability to retain dust particles from the air. The analysis of experimentally obtained data on the crown area showed that in the initial phases of ontogenesis, the average deviation in the crown area of plants does not depend on the place of growth. Due to artificial narrowing and sanitary pruning of the crown, as well as skeletal branches dying along the busiest highways, the values do not exceed 22–23 m² on average, with an allometric coefficient of 0.35–0.37. When comparing this coefficient in the control areas, the crown area in areas with a high level of anthropogenic load is 36 ± 11% lower. For trees growing under the conditions of the anthropogenic load of an industrial city and having reached the critical age, mechanical resistance varied depending on the study area and load level. At sites with a high level of pollution of the territory, a significant decrease in indicators was revealed in comparison with the control (mcr—71%, EI—75%, RRB—43%). Having analyzed all the obtained data, we can conclude that, until the age of 50–55 years, P. bolleana retains good viability, mechanical resistance, and general allometric ratios, upon which the stability of the whole plant depends. Even with modern approaches and tendencies toward landscaping with exotic introductions, it is necessary to keep P. bolleana as the main species in dendrobanocenoses. Full article

Peucedanum ostruthium (L.) W.D.J. Koch (Apiaceae) is a perennial herb native to alpine regions that is renowned in traditional medicine. This study provided a pharmacognostic evaluation, comparing the EOs obtained from its rhizomes and leaves (REO and LEO, respectively). A micromorphological analysis, which was carried out using fluorescence and scanning electron microscopy, revealed terpenoid-rich secretory ducts in both organs. The EOs were extracted by hydrodistillation and characterized by gas chromatography, coupled with flame ionization detection and mass spectrometry (GC-FID and GC-MS), revealing distinct chemical profiles. REO was dominated by monoterpenes (80.08%), especially D-limonene (29.13%), sabinene (19.77%), and α-phellandrene (12.02%), while LEO was sesquiterpene-rich (81.15%), with β-caryophyllene (21.78%), β-selinene (14.09%), and germacrene D (10.43%) as the major compounds. The in vitro assays demonstrated that both EOs exhibit significant antioxidant and anti-inflammatory activities, with LEO consistently outperforming REO across all tests. However, neither EO showed antimicrobial effects against common bacterial or fungal strains. This may have been due to the absence of polar antimicrobial constituents, such as coumarins, which are poorly recovered by hydrodistillation. To fully exploit the therapeutic potential of P. ostruthium, especially its antimicrobial properties, future studies should aim to develop integrated formulations combining volatile and non-volatile fractions, preserving the complete plant complex and broadening bioactivity. Full article

The use of Google Earth Engine (GEE), a cloud-based computing platform, in spatio-temporal evaluation studies has increased rapidly in natural sciences such as forestry. In this study, Sentinel-2 satellite imagery and Shuttle Radar Topography Mission (SRTM) elevation data and image classification algorithms based on two machine learning techniques were examined. Random Forest (RF) and Classification and Regression Trees (CART) were used to classify land use and land cover (LULC) in western Oregon (USA). To classify the LULC from the spectral bands of satellite images, a composition consisting of vegetation difference indices NDVI, NDWI, EVI, and BSI, and a digital elevation model (DEM) were used. The study area was selected due to a diversity of land cover types including research forest, botanical gardens, recreation area, and agricultural lands covered with diverse plant species. Five land classes (forest, agriculture, soil, water, and settlement) were delineated for LULC classification testing. Different spatial points (totaling 75, 150, 300, and 2500) were used as training and test data. The most successful model performance was RF, with an accuracy of 98% and a kappa value of 0.97, while the accuracy and kappa values for CART were 95% and 0.94, respectively. The accuracy of the generated LULC maps was evaluated using 500 independent reference points, in addition to the training and testing datasets. Based on this assessment, the RF classifier that included elevation data achieved an overall accuracy of 92% and a kappa coefficient of 0.90. The combination of vegetation difference indices with elevation data was successful in determining the areas where clear-cutting occurred in the forest. Our results present a promising technique for the detection of forests and forest openings, which was helpful in identifying clear-cut sites. In addition, the GEE and RF classifier can help identify and map storm damage, wind damage, insect defoliation, fire, and management activities in forest areas. Full article

Butterflies (Lepidoptera: Papilionoidea), considered bioindicators of habitat conservation status, are one of the groups used to assess the impact of habitat management and improvement efforts in the Biological Station of Torretes (Ibi, Alicante, Spain). This area also houses the Botanical Garden of the University of Alicante. The study was focused on the period from autumn 2022 to late spring 2023, and data on butterfly diversity and abundance from the current study were compared with historical data. Our results highlight that open spaces and the availability of trophic resources (nutritional plants) were key factors influencing the richness and abundance of Papilionoidea. The program for the creation and maintenance of new habitats at the biological station has increased spatial heterogeneity and the availability of trophic resources, which has led to a significant increase in the richness and abundance of butterflies in a short period. To support the conservation of Mediterranean ecosystems, and entomofauna in general, the Papilionidae are proposed as model groups for designing conservation projects based on traditional habitat management. These projects should promote spatial heterogeneity, include programs to enrich plant diversity, and be complemented by environmental education and outreach initiatives. Full article

This study reports seven new records of Curcuma L. (Zingiberaceae) for the flora of Laos: Curcuma comosa Roxb., C. pedicellata (Chaveer. & Mokkamul) Škorničk., C. phrayawan Boonma & Saensouk, C. pierreana Gagnep., C. rangjued Saensouk & Boonma, C. sabhasrii Saensouk, Maknoi, Wongnak & Rakarcha, and C. wanenlueanga Saensouk, Thomudtha & Boonma. Field surveys were conducted across various habitats in Laos, and species identification was confirmed through morphological comparisons with type specimens and protologues. An identification key for Curcuma species in Laos is provided to facilitate future taxonomic studies. Additionally, the lectotypification of C. comosa Roxb. is designated to ensure nomenclatural stability and clarify its taxonomic placement. The discovery of these species expands the known distribution of Curcuma in Indochina and highlights the importance of continued floristic exploration in understudied regions. These findings emphasize the need for biodiversity conservation, particularly in the nnatural habitats where these species occur. Furthermore, some of the newly recorded species exhibit attractive floral characteristics, making them valuable for sustainable horticultural applications, especially in ornamental plant cultivation. This study underscores the significance of taxonomic research in documenting regional flora, supporting conservation efforts, and promoting the sustainable utilization of plant diversity. Full article