Search Results (6,450)

Mentha essential oils (EOs), renowned for their distinctive aromas and diverse biological activities, represent a key focus in phytochemical and pharmacological research. While numerous reviews have documented the general properties of mint EOs, a systematic and critical synthesis of recent advances linking chemotypic diversity to biosynthetic mechanisms and evidence-based health benefits remains lacking. This review aims to address this gap by comprehensively analyzing the structural variability of EOs across major Mentha species, elucidating the chemotype-dependent enzymatic and genetic regulation within the plastidial methylerythritol phosphate (MEP) pathway, and evaluating preclinical and clinical evidence supporting their health-promoting activities, including antimicrobial, antioxidant, anti-inflammatory, digestive, respiratory, cognitive-enhancing, and anticancer effects. By integrating findings from cutting-edge transcriptomic and genomic studies, we highlight how genetic variations and epigenetic factors influence monoterpene biosynthesis and ultimately shape bioactivity profiles. Furthermore, we critically assess challenges related to EO standardization, bioavailability, and clinical translation, and propose interdisciplinary strategies, such as metabolic engineering, nano-delivery systems, and structured clinical trial designs to overcome these barriers. This review not only consolidates the current understanding of Mentha Eos, but also provides a forward-looking perspective on their potential applications in functional foods, pharmaceuticals, and personalized health products. Full article

Background: Growing evidence highlights the beneficial effects of flavonoids, including anthocyanins, as key components in reducing cardiovascular risk, and emphasizes that incorporating anthocyanin-rich fruits into the daily diet significantly impacts public health. Methods: The effect of bioactive polyphenols from raspberry fruit (RBF) on molecular pathways in inflammation was studied in activated RAW 264.7 macrophages and their protective potential against endothelial dysfunction was analyzed using TNF-α-induced human umbilical vein endothelial cells (HUVECs). Results: The results have shown that RBF extract, along with its anthocyanin and polyphenol fractions, has a significant anti-inflammatory effect in macrophage cell culture by inhibiting the LPS-induced expression of pro-inflammatory genes, including IL-6, IL-1β, TNF-α, and NF-κB. Moreover, RBF and both fractions have demonstrated a protective effect on endothelial function by decreasing the expression of several inflammation-related genes and adhesion molecules, such as IL-6, IL-1β, VCAM-1, ICAM-1, and SELE, in TNF-α-induced HUVECs. Conclusions: The consumption of RBF and/or polyphenol-rich extracts may help prevent the onset of early atherosclerosis. This is attributed to their ability to improve inflammation status and enhance vascular endothelial function. Given the strong anti-inflammatory properties of RBF, incorporating them into a daily diet could significantly reduce the risk of non-communicable diseases related to inflammation. Full article

Frozen shoulder (FS), traditionally regarded as an idiopathic musculoskeletal disorder characterized by pain, stiffness, and capsular fibrosis, is increasingly recognized as the clinical manifestation of systemic endocrine, metabolic, vascular, and immunological dysfunctions. This narrative review reframes FS within a broader neuro–endocrine–immunometabolic model, emphasizing the central role of estrogen deficiency, resistance, and receptor-level disruption, together with their interactions with thyroid dysfunction, endothelial health, and lifestyle-related low-grade inflammation (LGI). Evidence from epidemiological, clinical, and mechanistic studies shows that estrogen signaling failure weakens anti-inflammatory, antifibrotic, and antioxidant defenses, predisposing peri- and postmenopausal women to more severe FS phenotypes. Thyroid dysfunction, particularly hypothyroidism, further contributes to fibrosis and pain sensitization. Endothelial dysfunction—driven by poor diet, advanced glycation end-products (AGEs), and oxidative stress—impairs vascular integrity and promotes local microvascular inflammation. In parallel, lifestyle factors such as sedentarism, circadian misalignment, psychosocial stress, and environmental exposures sustain systemic LGI and hormonal resistance. Together, these interconnected mechanisms suggest that FS is not merely a localized joint pathology but a systemic disorder requiring integrative clinical strategies that combine orthopedic management with endocrine evaluation, metabolic monitoring, dietary interventions, circadian health, and stress regulation. In addition, this review outlines specific clinical implications, highlighting how an integrative, personalized approach that targets hormonal, metabolic, vascular, and lifestyle dimensions may improve pain, function, and long-term prognosis in FS. This paradigm shift underscores the need for future research to focus on stratified patient profiling and interventional trials targeting hormonal, vascular, and lifestyle axes to improve outcomes, particularly in women who remain disproportionately affected by FS. Full article

Alkylic molecules are found as some of the main components of natural essential oils. These essential oils offer several therapeutic properties in skin treatments and cosmetics. Systems providing controlled release of these molecules through the skin tissue are a challenge for their applications. This work explores some properties of the crystal structure of α-pinene and the adsorption and desorption of five terpenoid components of essential oils, such as α-pinene, limonene, β-ocimene, β-caryophyllene, and β-elemene, in the confined surfaces provided by natural clay minerals, particularly montmorillonite (MNT). These terpenoids have a methyl-ethenyl group as their common structural feature. Molecular modelling calculations have been applied at the atomic scale, including force fields, quantum mechanical methods, and molecular dynamics simulations. We calculated the crystallographic and spectroscopic properties of the α-pinene crystal via density functional theory (DFT)-level calculations, which were very close to the known experimental data. Moreover, this work explored the adsorption and desorption of these molecules in confined surfaces provided by MNT. Molecular dynamics simulations also showed the adsorption of these organics in the confined interlayer space of MNT at room temperature and allowed us to know the diffusion coefficient of these adsorbates in this material. The direct adsorption process of these molecules in the vapour phase is not energetically favourable, suggesting the use of non-aqueous solvents and kinetics and thermodynamic conditions for this process. However, the release of these molecules into aqueous media are energetically favourable, predicting that MNT–essential oil can be an excellent pharmaceutical formulation to be delivered in skin as a bioactive preparation with anti-inflammatory or cosmetic power. This research was performed to predict possible therapeutic applications for future experimental works. Full article

Oxidative stress occurs when excessive production of reactive oxygen species (ROS) disrupts the redox balance between oxidants and antioxidants. The brain is particularly vulnerable to oxidative stress due to its high metabolic rate. Astrocytes, the key homeostatic cells in the brain, play a crucial role in maintaining physiological function, including the regulation of oxidative stress. In the present study, we investigated whether hispidulin can mitigate oxidative damage by regulating redox imbalance, inflammatory signaling and apoptotic response in hydrogen peroxide (H₂O₂)-treated C6 astroglial cells. The cells were exposed to hispidulin at various concentrations for 24 h and then challenged with H₂O₂ for another 24 h. Hispidulin treatment significantly increased the viability in all concentrations and attenuated H₂O₂-induced increases in ROS production, lactate dehydrogenase release, and nitric oxide levels. Furthermore, it significantly downregulated proinflammatory markers, including tumor necrosis factor α, interleukin-6 (IL-6), and IL-1β. Western blot analysis exhibited that hispidulin significantly increased the antioxidant defense system-related proteins such as nuclear factor erythroid 2-related factor 2, glutathione peroxidase 1, and superoxide dismutase. In addition, hispidulin decreased the pro-apoptotic Bax and cytochrome C, while increasing the levels of anti-apoptotic Bcl-2. In conclusion, hispidulin showed a protective effect against H₂O₂-induced injury in C6 astroglial cells by suppressing oxidative stress, inflammation, and apoptosis. Full article

Mushroom diversity is essential for maintaining ecological balance and provides valuable bioactive compounds for human use. Beyond their nutritional value, mushrooms contribute to functional foods and have applications in nutraceuticals, pharmaceuticals, and biotechnology. For example, β-glucans from Lentinula edodes are commercialized as immune-enhancing nutraceuticals, polysaccharide Krestin (PSK) from Trametes versicolor is used as an adjuvant in cancer therapy, and enzymes such as laccases from Pleurotus species are widely applied in biotechnological processes. One of the abundant compounds found in mushrooms is ergosterol, which is a sterol present in the cell membrane of the fungal body. Ergosterol has significant health benefits due to its antioxidant, immunomodulatory, and anti-inflammatory properties. Furthermore, ergosterol is a precursor to vitamin D₂ (ergocalciferol), which can be synthesized through exposure to ultraviolet (UV) light and thermal radiation. This review highlights the importance of Himalayan mushroom biodiversity, particularly the wild edible mushrooms traditionally collected and used. This review thoroughly discusses the ergosterol and vitamin D₂ content, their biosynthesis in mushrooms, and the role of environmental factors used to enhance biosynthesis. We also discuss the sustainable cultivation of Himalayan mushrooms and their nutraceutical properties. Several Himalayan mushrooms have been reported to possess health-promoting properties, and their incorporation into functional foods may contribute to improved public health. Furthermore, the future research directions are highlighted. Full article

Chronic kidney disease (CKD) causes a variety of health problems including renal dysfunction and cardiovascular disease. This study aimed to investigate whether the probiotic strain Lactiplantibacillus plantarum HY7718 (HY7718) can protect against CKD using HK2 cells and a CKD mouse model, generated by feeding mice a diet containing 0.15% adenine. In vitro tests showed that HY7718 was anti-inflammatory in H₂O₂-treated HK2 cells and reduced apoptosis of tumor necrosis factor-α/cycloheximide-induced HK2 cells. In the adenine-induced CKD model, markers of renal dysfunction (blood urea nitrogen (BUN) and creatinine (Crea)) and inorganic calcium and phosphorus were markedly increased. However, oral administration of HY7718 (10⁸ colony-forming units/kg/day) significantly attenuated these increases. HY7718 also reduced the kidney histopathological score, including tubular necrosis, cast formation, and tubular dilatation, as well as the mononuclear cell infiltration score in kidney tissue, suggesting that it could reverse the progression of CKD. Additionally, HY7718 downregulated the renal expression of pro-inflammatory cytokine genes and members of the TLR/NF-κB signaling pathway. Furthermore, HY7718 reduced tubule apoptotic cells and expression of apoptosis-related genes, indicating that it is potentially renoprotective. These results demonstrate that supplementation with the probiotic HY7718 can ameliorate CKD symptoms by improving renal function and reducing kidney injury. Full article

Chronic dermatological conditions such as acne vulgaris, androgenetic alopecia (AGA), and alopecia areata (AA) affect hundreds of millions worldwide and contribute substantially to quality-of-life impairment. Despite the availability of systemic retinoids, anti-androgens, and JAK inhibitors, therapeutic responses remain heterogeneous and relapse is common, underscoring the need for biologically grounded stratification. Over the past decade, large genome-wide association studies and functional analyses have clarified disease-specific and cross-cutting mechanisms. In AA, multiple independent HLA class II signals and immune-regulatory loci such as BCL2L11 and LRRC32 establish antigen presentation and interferon-γ/JAK–STAT signalling as central drivers, consistent with clinical responses to JAK inhibition. AGA is driven by variation at the androgen receptor and 5-α-reductase genes alongside WNT/TGF-β regulators (WNT10A, LGR4, RSPO2, DKK2), explaining follicular miniaturisation and enabling polygenic risk prediction. Acne genetics highlight an immune–morphogenesis–lipid triad, with loci in TGFB2, WNT10A, LGR6, FASN, and FADS2 linking follicle repair, innate sensing, and sebocyte lipid metabolism. Barrier modulators such as FLG and OVOL1, first described in atopic dermatitis, further shape inflammatory thresholds across acne and related phenotypes. Together, these findings position genetics not as an abstract catalogue of risk alleles but as a map of tractable biological pathways. They provide the substrate for patient-stratified interventions ranging from JAK inhibitors in AA, to endocrine versus morphogenesis-targeted strategies in AGA, to lipid- and barrier-directed therapies in acne, while also informing cosmetic practices focused on barrier repair, sebaceous balance, and follicle health. Full article

This study investigated the contamination, composition, and functional properties of Wolffia globosa from northern Thailand. The results showed that the heavy metal content of dried W. globosa complied with Thai regulations, ensuring its safety. Its proximate analysis revealed high protein levels with lysine, leucine, and phenylalanine as the principal essential amino acids. The protein was effectively extracted using the alkaline extraction method, followed by precipitation induced by acid or heat. The precipitates and supernatants resulting from various acid- or heat-induced protein precipitation were obtained. The highest protein content was found in the pH 3 precipitate (51.15 ± 6.71%). In contrast, the pH 5 supernatant exhibited the most potent antioxidant activities (2.22 ± 0.05 mmol Trolox/mg and 4.55 ± 0.18 mmol Fe²⁺/mg), as determined by ABTS and FRAP assays, respectively. Additionally, a strong correlation was observed between phenolic content and antioxidant activity. Both supernatant and precipitate protein extracts from W. globosa exhibited no cytotoxicity in THP-1 cells and displayed anti-inflammatory effects by decreasing the production of IL-1β and IL-6. They also downregulated phospho-NF-κB, phospho-IκB-α, and COX-2, consistent with reduced NF-κB pathway activation. These findings position W. globosa as a promising, sustainable plant-based protein with bioactive and functional properties, making it a viable candidate for functional food formulations that enhance dietary health and add value to local agricultural resources. Full article

Propolis, a resinous substance produced by Apis mellifera, is a chemically diverse natural product rich in polyphenols, flavonoids, terpenes, vitamins, and minerals. These compounds exhibit a range of biological activities, including antimicrobial, antioxidant, antidiabetic, anti-inflammatory, and cardioprotective effects, making propolis an attractive candidate for applications in the food and health sciences. This review summarizes the current understanding of its chemical composition and the environmental, botanical, and genetic factors influencing its variability. Particular attention is given to extraction methods: while conventional approaches such as maceration and Soxhlet extraction remain widely used, they often compromise compound stability. In contrast, emerging techniques—such as ultrasound-, microwave-, and supercritical fluid-assisted extraction—enhance yield, selectivity, and sustainability. Encapsulation strategies, including micro- and nanoencapsulation, are also explored as practical tools to protect propolis bioactives from degradation, improve solubility, and mask their strong taste, thereby ensuring higher bioavailability and consumer acceptability. Recent applications in the meat, dairy, beverage, bakery product, and edible film industries demonstrate propolis’ potential to extend shelf life, inhibit microbial growth, and enrich the nutritional and functional quality of these products. Nevertheless, challenges remain, particularly regarding standardization, allergenicity, dosage, and regulatory approval, which limit its widespread industrial adoption. Overall, Apis mellifera propolis represents a multifunctional natural ingredient that bridges traditional medicine with modern food science. Advances in extraction and encapsulation technologies are paving the way for the integration of this ingredient into functional foods, nutraceuticals, and sustainable food preservation systems, underscoring its value as a natural alternative to synthetic additives. Full article

Background: High-fat diet (HFD)-induced hepatic inflammation impairs liver function, promotes fibrosis, and may progress to hepatocellular carcinoma, thereby posing a significant threat to human health. Meanwhile, fermented whole grains have attracted growing attention owing to their diverse beneficial biological properties. Methods: In this study, we investigated the effects of co-fermented quinoa and black barley (FG) on HFD-induced chronic hepatic inflammation using male C57BL/6J mice. Results: FG intervention significantly attenuated excessive body weight gain and reduced hepatic adipose accumulation in HFD-fed mice. Furthermore, FG alleviated hepatic inflammation by downregulating the transcriptional and protein levels of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and interleukin-6 (IL-6), as well as the transcriptional levels of toll-like receptor 4 (Tlr4), cluster of differentiation 14 (CD14), and myeloid differentiation primary response gene 88 (Myd88). Metabolomic analysis identified several hepatic and fecal metabolites, such as vitamin A and L-tryptophan, that were upregulated by FG treatment. The strong negative correlation of these metabolites with hepatic inflammatory markers suggests their role as putative mediators of FG’s anti-inflammatory action. Additionally, FG enhanced the relative abundances of probiotic taxa, including g_Lawsonibacter, g_Acetatifactor, and s_Bifidobacterium cricetid, and upregulated the microbial bile acid (BA) biosynthesis pathway. Notably, these enriched probiotics exhibited a positive correlation with the aforementioned fecal metabolites. Conclusions: Our findings suggest that FG has the potential to alleviate HFD-induced hepatic inflammation by restoring gut microbiota imbalance and reversing metabolic disorders. Full article

Fungal-derived bioactive compounds are emerging as key components in functional food development, offering new opportunities for health-promoting formulations. The Polyporaceae family, particularly Cerrena unicolor, has demonstrated significant potential due to its rich biochemical profile and diverse health benefits. Despite its extensive bioactive properties, its application in food science and biotechnology remains underutilized. This review explores the bioactive composition, technological potential, and functional applications of C. unicolor in innovative food systems. We analyze its antioxidant, antimicrobial, and anticancer effects, focusing on its interactions with dairy-based matrices to enhance bioavailability and therapeutic potential. C. unicolor is a valuable source of polysaccharides, phenolics, and enzymatic compounds with antioxidant and anti-inflammatory properties. Its anticancer potential, especially when incorporated into dairy fermentations, opens new avenues for oncology-focused functional foods. Strong antimicrobial activity suggests its potential as a natural biopreservative or bioactive food additive. Bioactive fractions contribute to metabolic health improvements (diabetes management) and tissue regeneration, highlighting their role in next-generation nutraceuticals. Incorporating C. unicolor into functional food systems represents a cutting-edge approach to biotechnology-driven health solutions. Further research is required to optimize its formulation, improve bioavailability, and explore regulatory pathways for market implementation. Full article

Curcumin (Cur), a natural polyphenolic compound with potent antioxidant and anti-inflammatory properties, faces significant challenges in cosmeceutical applications due to its poor aqueous solubility and low bioavailability. Nanotechnology offers a promising approach to overcome these limitations and enhance the functionality of cosmetic formulations. In this work, Cur-loaded nanoemulsions (NEs) were developed using a droplet microfluidics technique to enhance Cur’s stability, bioavailability, and permeability for advanced cosmeceuticals. Various oils were screened for Cur solubility, with coconut oil demonstrating the highest capacity. Optimal oil-to-water flow ratios were determined to produce monodisperse NEs with controlled droplet sizes. Characterization via dynamic light scattering (DLS) revealed stable NEs with Z-potential values exceeding −30 mV at both room temperature and +4 °C for up to 21 days, indicating strong colloidal stability. Antioxidant activity was evaluated through DPPH assays, while in vitro permeability studies of the drug-loaded NEs after incorporation into suitable hydrogels, using Strat-M^® membranes mimicking human skin, demonstrated significantly enhanced penetration of the encapsulated Cur. In sum, this work highlights the potential of droplet microfluidics as a scalable and precise method for producing high-performance Cur NEs tailored for cosmeceutical applications. Full article

Rheumatoid arthritis (RA) is characterized by chronic inflammation mediated by the JAK/STAT pathway. Variants in STAT4 have been associated with autoimmune susceptibility, but their functional role in RA remains unclear. The aim of this study was to genetically and functionally characterize STAT4 in RA patients with varying disease activity by analyzing two variants, mRNA expression, phosphorylated STAT4 (pSTAT4), and inflammatory cytokines (IL-12, IL-23, and IFN-γ). Sixty-three Mexican patients with RA were stratified into remission/low and moderate/high activity groups. Genotyping, STAT4 mRNA expression, pSTAT4 quantification, cytokine profiling, and treatment analyses were conducted. Patients receiving methotrexate, hydroxychloroquine, and sulfasalazine had higher IL-12 concentrations compared with those on other regimens. In remission/low activity patients, GC/GC carriers exhibited increased IL-12, PBMC levels, and anti-CCP antibodies, while GC/TT carriers in the moderate/high activity group showed distinct ESR values. Secondary analyses revealed that TT/TT carriers with STAT4 overexpression exhibited higher IFN-γ and IL-23 levels. IL-12 differences persisted among GC/GC carriers regardless of STAT4 expression status. In conclusion, these exploratory findings suggest potential interactions among STAT4 haplotypes, expression status, and treatment regimens influencing cytokine and inflammatory profiles in RA. However, due to the small subgroup sizes, the observed associations should be interpreted with caution and considered hypothesis-generating until validated in larger cohorts. Full article

Environmental sensitivity illnesses—including fibromyalgia syndrome (FMS), myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS), and multiple chemical sensitivity (MCS)—are chronic, disabling disorders characterized by hypersensitivity to environmental stimuli, persistent fatigue, widespread pain, and neurocognitive and autonomic dysfunction. Although their diagnostic criteria differ, increasing evidence suggests overlapping clinical features and shared biological mechanisms. A unifying hypothesis highlights the gut–brain–immune axis, where alterations in the intestinal microbiome, epithelial barrier dysfunction, and aberrant immune signaling interact with central sensitization and systemic metabolic dysregulation. Recent studies demonstrate reduced microbial diversity, depletion of anti-inflammatory taxa (e.g., Faecalibacterium prausnitzii, Bifidobacterium), and enrichment of pro-inflammatory Clostridium species across these conditions. These shifts likely alter production of short-chain fatty acids, amino acid metabolites, and complex lipids, with downstream effects on mitochondrial function, neuroinflammation, and host energy metabolism. Moreover, emerging clinical interventions—including probiotics, prebiotics, synbiotics, and fecal microbiota transplantation—suggest a potential role for microbiome-targeted therapies, though controlled evidence remains limited. This review synthesizes current knowledge on microbiome alterations in FMS, ME/CFS, and MCS, emphasizing their convergence on metabolic and immune pathways. By integrating microbial, immunological, and neurophysiological perspectives, we propose a microbiome-centered framework for understanding environmental sensitivity illnesses and highlight avenues for translational research and therapeutic innovation. Full article