Search Results (271)

Maritime surveillance is critical for ensuring the safety and continuity of sea logistics, port operations, and coastal activities in the presence of anomalies such as unlawful maritime activities, security-related incidents, and anomalous events (e.g., tsunamis or aggressive marine wildlife). Recent advances in unmanned aerial vehicles (UAVs)/drones and computer vision enable automated, wide-area monitoring that can reduce dependence on continuous human observation and mitigate the limitations of traditional methods in complex maritime environments (e.g., waves, ship clutter, and marine animal movement). This study proposes a hybrid anomaly detection and tracking pipeline that integrates YOLOv12, as the primary object detector, with two auxiliary modules: (i) motion assistance for tracking moving anomalies and (ii) stillness (appearance) assistance for tracking slow-moving or stationary anomalies. The system is trained and evaluated on a custom maritime dataset captured using a DJI Mini 2 drone operating around a port area near Bayshore MRT Station (TE29), Singapore. Windsurfers are used as proxy (dummy) anomalies because real anomaly footage is restricted for security reasons. On the held-out test set, the trained model achieves over 90% on Precision, Recall, and mAP50 across all classes. When deployed on real maritime video sequences, the pipeline attains a mean Precision of 92.89% (SD 13.31), a mean Recall of 90.44% (SD 15.24), and a mean Accuracy of 98.50% (SD 2.00%), indicating strong potential for real-world maritime anomaly detection. This proof of concept provides a basis for future deployment and retraining on genuine anomaly footage obtained from relevant authorities to further enhance operational readiness for maritime and coastal security. Full article

Cervical spinal cord injury causes severe functional impairment with limited spontaneous recovery, and while spinal cord stimulation has emerged as a promising neuromodulatory strategy, evidence for cervical applications remains fragmented. To address this gap, we conducted a systematic review synthesizing preclinical and clinical evidence on cervical spinal cord stimulation for functional rehabilitation following spinal cord injury. The review was registered on PROSPERO (CRD420251088804) and conducted in accordance with PRISMA guidelines, with PubMed, Embase, IEEE Xplore, and Web of Science searched from inception to July 2025 for animal and human studies of cervical spinal cord stimulation, including epidural, intraspinal, and transcutaneous approaches, reporting functional neurological outcomes. Risk of bias was assessed using the Cochrane RoB 2 and ROBINS-I tools, and due to substantial heterogeneity, results were synthesized narratively. Thirty-one studies comprising 119 animals and 156 human participants, met inclusion criteria. Across studies, outcome measures such as GRASSP, ISNCSCI, and dynamometry consistently demonstrated improvements in hand strength, dexterity, and voluntary motor activation. Several studies also reported gains in sensory and autonomic function, whereas respiratory outcomes were infrequently assessed. Adjunctive interventions, including cortical stimulation, brain–computer interface priming, and task-specific training frequently augmented recovery. Adverse events were generally mild, although overall risk of bias was predominantly serious. Overall, cervical spinal cord stimulation demonstrates preliminary assistive and therapeutic effects on motor recovery, with additional sensory, autonomic, and potential respiratory benefits. Full article

Shelter environments frequently expose dogs to chronic stress and anxiety, which can compromise their welfare and reduce their chances of adoption. Recent interest in psychedelic-assisted approaches has suggested potential therapeutic applications in veterinary behavioral medicine, although empirical evidence remains scarce. This study aimed to evaluate the combined effects of low-dose 1-cyclopropionyl lysergic acid diethylamide (1cp-LSD), a legal lysergamide prodrug of LSD in several countries, and ethological intervention (EI) on the behavior and welfare of shelter dogs. Twenty dogs were randomly assigned to four groups: pharmacological intervention, ethological intervention, combined treatment, or control. The ethological sessions were conducted by veterinary behaviorists, and pharmacological treatment consisted of 10 µg of 1cp-LSD administered orally for three weeks. Blinded evaluators assessed animals using validated anxiety and welfare scales, including a treatment expectation scale, before, during and after the intervention. Results showed that the combined condition consistently outperformed single interventions, significantly enhancing sociability, calmness, and positive emotional reactivity. Importantly, these improvements persisted for three weeks following treatment cessation, indicating sustained benefits beyond the active intervention phase. These findings provide preliminary evidence for the potential of integrating low doses of psychedelics with behavioral therapy in shelter settings. Future studies with larger cohorts and refined pharmacokinetic data are required to confirm safety, elucidate mechanisms, and optimize protocols for clinical application in veterinary practice. Full article

Background: Hospitalisation for paediatric oncohaematological diseases entails significant physical and psychological stress, often compromising children’s emotional regulation and daily functioning. In this context, complementary interventions can provide additional support to the clinical work conducted with children and adolescents undergoing treatment, fostering emotional awareness and well-being. This study evaluates the feasibility and perceived benefits of an animal-assisted psychoeducational intervention to enhance emotional coping during hospitalisation. Methods: A single-centre observational study was conducted at Regina Margherita Children’s Hospital in Italy in collaboration with the ZOOM Foundation (Turin, Italy), between September 2023 and May 2025. Sixty patients aged 6–15 years participated in an intervention combining observation of an in-ward aquarium, virtual animal encounters, and completion of a psychoeducational booklet promoting emotional reflection through symbolic identification with animal behaviours. Results: The intervention showed high feasibility and acceptability: 90% of participants (54/60) reported positive emotions and enjoyment, 80% (48/60) found the booklet engaging, and all participants (100%) perceived care and attention from facilitators. The activities fostered engagement, curiosity, and well-being, though their impact on deeper emotional processing appeared limited. Conclusions: Animal-assisted psychoeducational interventions are feasible and well-received in paediatric oncohaematology, offering complementary support to clinical care by promoting emotional resilience and enriching the hospital experience. Full article

Women of reproductive age, especially those with polycystic ovarian syndrome (PCOS), often use glucagon-like peptide-1 receptor agonists (GLP-1RAs) to improve their metabolic functions. A growing body of evidence suggests that GLP-1R signaling may directly affect ovarian physiology, influencing granulosa cell proliferation, survival pathways, and steroidogenic production, in addition to its systemic metabolic effects. Nonetheless, there is a limited comprehension of the molecular mechanisms that regulate these activities and their correlation with menstrual function, reproductive potential, and assisted reproduction. This comprehensive review focuses on ovarian biology, granulosa cell signaling networks, steroidogenesis, and translational fertility outcomes, integrating clinical, in vivo, and in vitro information to elucidate the effects of GLP-1 receptor agonists on reproductive health. We conducted a thorough search of PubMed, Scopus, and Web of Science for randomized trials, prospective studies, animal models, and cellular experiments evaluating the effects of GLP-1RA on reproductive or ovarian outcomes, in accordance with PRISMA criteria. The retrieved data included metabolic changes, androgen levels, monthly regularity, ovarian structure, granulosa cell growth and death, FOXO1 signaling, FSH-cAMP-BMP pathway activity, and fertility or IVF results. Clinical trials shown that GLP-1 receptor agonists improve menstrual regularity, decrease body weight and central adiposity, increase sex hormone-binding globulin levels, and lower free testosterone in overweight and obese women with PCOS. Liraglutide, when combined with metformin, significantly improved IVF pregnancy rates, whereas exenatide increased natural conception rates. Mechanistic studies demonstrate that GLP-1R activation affects FOXO1 phosphorylation, hence promoting granulosa cell proliferation and anti-apoptotic processes. Incretin signaling altered steroidogenesis by reducing the levels of StAR, P450scc, and 3β-HSD, so inhibiting FSH-induced progesterone synthesis, while simultaneously enhancing BMP-Smad signaling. Animal studies demonstrated both beneficial (enhanced follicular growth, anti-apoptotic effects) and detrimental results (oxidative stress, granulosa cell death, uterine inflammation), indicating a context- and dose-dependent response. GLP-1 receptor agonists influence female reproductive biology by altering overall physiological processes and specifically impacting the ovaries via FOXO1 regulation, steroidogenic enzyme expression, and BMP-mediated FSH signaling. Preliminary clinical data indicate improved reproductive function in PCOS, as seen by increased pregnancy rates in both natural and IVF cycles; nevertheless, animal studies reveal a potential risk of ovarian and endometrial damage. These results highlight the need for controlled human research to clarify reproductive safety, molecular pathways, and optimum therapy timing, particularly in non-PCOS patients and IVF settings. Full article

RECK was first reported as a transformation suppressor gene in 1998 and gradually gained attention as evidence indicating its reduced expression in a wide variety of human cancers accumulated. RECK encodes a membrane-anchored glycoprotein exhibiting protease inhibitor activity against matrix metalloproteases. Restored expression of RECK in cancer xenograft models suggests it suppresses tumor growth and/or metastasis. RECK was also found to be essential for mammalian embryogenesis, especially in the maintenance of tissue integrity as well as the development of neural and vascular systems. Due to its functional versatility during animal development, we only recently began to obtain formal experimental evidence that RECK is a bona fide tumor suppressor. In the meantime, mechanisms by which RECK expression is reduced in cancer cells have been explored. Various stimuli that alter RECK expression have also been described. Furthermore, recent findings in the clinic as well as in animal studies indicate the involvement of RECK in disorders other than cancer. The aim of this article is to summarize our current knowledge of RECK and assist future efforts to understand its nature and functions and to develop useful applications. Full article

In African countries and many developing countries, communal farmers rely on livestock such as cattle, goats, and sheep to support food security, income, and agricultural activities. Fertility in these animals is often limited by poor semen quality, which reduces sperm concentration, total motility, and morphology. Assisted reproductive biotechnologies, including semen cryopreservation and artificial insemination, are increasingly essential to enhance reproductive efficiency and productivity. Although cryopreservation preserves valuable genetic material, it can damage sperm cells, making high-quality extenders critical for protection. Common extenders, such as Tris-egg yolk glucose, citrate-sugar-based, and skimmed milk solutions, supply nutrients and protect sperm membranes. To further minimize oxidative stress, antioxidants are incorporated, with growing interest in plant-derived compounds. Many plants contain bioactive substances, including antioxidants and phytomelatonin, which can enhance sperm quality safely and effectively. This review examines the use of plant-based antioxidants during semen cryopreservation and highlights their potential to improve fertility in mammalian livestock. Full article

Background: Effective communication is a fundamental competency in veterinary medicine that shapes the quality of veterinarian–client relationships, shared decision-making, and animal welfare. However, consistent and systematic integration of communication training across veterinary curricula remains uneven worldwide. Methods: This scoping review mapped and analyzed educational programs aimed at developing communication competencies in veterinary education and professional practices. A systematic search was conducted according to PRISMA-ScR guidelines, identifying 37 eligible studies published between 2005 and 2024. Results: Most publications were in English and originated from North America, particularly Canada (n = 15) and the United States (n = 8). Regarding target populations, 15 studies (40.5%) focused on veterinary students, 12 (32.4%) on practicing veterinarians, 8 (21.6%) on animal owners or clients, and 2 on veterinary educators. 18 studies (48.7%) described structured programs that used active learning strategies such as role-play, clinical simulations, peer-assisted learning, and formative feedback. The competencies frequently emphasized include empathy, active listening, nonverbal communication, conflict resolution, and rapport building. Notable best practices included the Calgary–Cambridge model, Objective Structured Clinical Examination (OSCE), and reflective video analysis. Conclusions: The available evidence indicates a growing emphasis on clinical communication within veterinary education, primarily implemented through experiential and practice-based approaches. However, substantial gaps persist in the representation of Latin American contexts and in the systematic, longitudinal integration of communication skills across veterinary curricula. Addressing these gaps may contribute to more coherent, equitable, and context-sensitive communication training in veterinary education. Full article

Background/Objectives: Evaluating the feasibility of randomized controlled trials (RCTs) represents a critical next step for advancing human–animal interaction (HAI) science and rigorously exploring the role of animal-assisted interventions (AAIs) in psychiatric acute care. This study presents strategies for conducting a pilot RCT comparing an animal-assisted intervention involving dogs (AAI) with an active conversational control (CC), which incorporated conversation with a human volunteer, and treatment as usual (TU) for improving mental health outcomes in psychiatrically hospitalized adults. Methods: We recruited participants from an acute-care psychiatric unit at an academic medical center. AAI and CC were delivered by volunteer handlers with and without their registered therapy dogs. Feasibility data included number of recruitment contacts, recruitment rate, and reasons for non-enrollment. We describe recruitment challenges encountered and mitigating strategies for successful study completion. Results: Recruitment occurred over 23 months with a goal of 60 participants participating in at least one intervention day. A total of 264 patients were referred to the study and 72 enrolled. The additional 12 people were recruited to replace participants who did not complete any intervention days and did not provide any intervention data. Study recruitment goals were met with a recruitment rate of 27.30%. Conclusions: Research to improve the lives of patients in acute psychiatric care is a vital public health goal, yet RCTs are difficult to conduct in acute care settings. Studies like this strengthen HAI and psychiatric science by providing a roadmap for implementing successful AAI RCT designs. Full article

Background/Objectives: Antimicrobial resistance (AMR) in wildlife is an emerging public health concern due to the risk of zoonotic transmission, especially through the food chain, yet data on free-ranging animals remain scarce. This study examined the presence and patterns of AMR among bacteria isolated from hunted wild boars in the Campania region of Italy. Methods: Matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) was used to identify bacterial isolates from wild boar meat and carcass swabs to the species level, and the Kirby–Bauer disk diffusion test was applied to screen 205 isolates, spanning 20 bacterial genera, against a panel of clinically relevant antibiotics. Resistance metrics were analyzed at genus and antibiotic levels, and patterns were visualized using a hierarchically clustered heatmap. Results: Resistance was detected in 15 of the 20 genera, with full susceptibility observed in Acinetobacter, Arthrobacter, Glutamicibacter, Leclercia, and Rahnella. Overall, 67.3% (138/205) of the isolates showed resistance to at least one antibiotic, with 33.7% (69/205) classified as multidrug-resistant (MDR). Carbapenems retained the highest activity (≥95% susceptibility) among all genera tested, while amoxicillin/clavulanate (78.4%) and aztreonam (57.4%) exhibited the highest mean resistance. Among potential pathogens, Escherichia coli exhibited an extended-spectrum β-lactamase (ESBL)-like phenotype, with resistance to amoxicillin/clavulanate (67%), aztreonam (54%), and ceftazidime (47%) but preserved carbapenem susceptibility. Staphylococcus spp. showed pronounced resistance to linezolid (57%) and erythromycin (52%), whereas Pseudomonas isolates demonstrated elevated resistance to aztreonam and ceftazidime (57% each). Opportunistic pathogens such as Alcaligenes faecalis and Pantoea agglomerans showed peak resistance to ciprofloxacin and amoxicillin/clavulanate. Pathogens and opportunistic pathogens demonstrated higher mean resistance (>30%) than commensals (≤32%), but the difference in mean and median resistance levels was not statistically significant (Mann–Whitney’s U test, W = 4, p = 0.39). Conclusions: These findings highlight the widespread occurrence of AMR and MDR phenotypes, with clinically significant resistance patterns in wild-boar-associated bacteria, including non-pathogenic strains, highlighting their role in the amplification of AMR. Although the preservation of carbapenem susceptibility underscores their potential as last-line antibiotics, the high resistance to commonly used antibiotics raises concerns for zoonotic transmission. Surveillance of wildlife reservoirs therefore remains critical for integrated AMR control. Full article

Peripheral nerve repair remains a major clinical challenge, and novel strategies such as conduit-assisted repair have been developed to improve outcomes. In this study, we fabricated a 3D-printed nerve guidance conduit (NGC) composed of polycaprolactone (PCL), a biocompatible and biodegradable polymer, combined with acellular dermal matrix (ADM) derived from porcine dermis, in order to create a multilayered PCL–ADM NGC with both favorable mechanical properties and biological activity. Twenty rabbits were divided into four groups: a negative control group, a silicone tube repair group, an autologous nerve graft group, and a group treated with the 3D-printed PCL–ADM NGCs. Sciatic nerve regeneration was assessed at 4 and 12 weeks postoperatively using electrophysiological measurements, histological staining, and electron microscopy. The PCL–ADM NGC demonstrated comparable axonal regeneration and functional recovery to autologous grafting, and it significantly outperformed silicone tubes in terms of axonal count and maximal electrophysiological response. Histological and ultrastructural analyses further confirmed that the PCL–ADM NGC facilitated organized regeneration with dense myelinated axons and reduced degenerative changes. The fabricated NGCs exhibited excellent flexibility without compromising lumen diameter, which is critical for adapting to the physiological environment of peripheral nerves. These findings indicate that combining synthetic polymers with biologically derived matrices can enhance the regenerative microenvironment and overcome limitations of traditional synthetic conduits. In conclusion, the 3D-printed PCL–ADM NGC represents a promising alternative to both silicone tube repair and autologous nerve grafting by providing structural support and bioactivity while reducing the need for donor nerve harvesting. Further studies in larger animal models and longer follow-up periods will be required to confirm long-term efficacy and support clinical translation of this technology. Full article

The ethical and legal governance of Animal-Assisted Interventions (AAIs) remains conceptually and normatively fragmented. Although animals engaged in therapeutic, educational, and assistive activities make valuable contributions to human well-being, they continue to be defined by law as property or welfare objects, despite their meaningful yet limited forms of relational participation within structured human-controlled environments. This perspective obscures their context-dependent responsiveness and their institutional embeddedness. The present paper addresses this gap by adopting a normative and interdisciplinary approach grounded in relational legal theory and vulnerability scholarship. The concept is developed by drawing on Jennifer Nedelsky’s notion of relational autonomy and Martha Fineman’s theory of universal vulnerability. This results in the conceptualisation of AAI animals as correlated subjects: beings whose ethical and legal significance derives from the relationships and institutional contexts that shape their participation. The analysis identifies weaknesses in current medico-legal practices that frame veterinary certification and welfare assessment as static technical acts, ignoring their relational and systemic dimensions. The paper puts forward a relational ethical–legal framework for Animal-Assisted Interventions, centred on relational vulnerability, context-sensitive oversight and continuous institutional accountability. A number of practical recommendations are put forward, including the introduction of renewable ethical licences, inter-institutional monitoring and the establishment of multidisciplinary oversight mechanisms. By redefining animals’ normative status through relational ethics, in alignment with the interconnected human, animal, and environmental dimensions emphasized by the One Welfare principles, the study advances a shift from welfare-based protection toward a model of justice grounded in interspecies interdependence and institutional responsiveness. Full article

Background/Objectives: Late-life depression is prevalent yet frequently underdiagnosed, underscoring the need for accessible and safe non-pharmacological approaches. Pet-assisted interventions, including live animal-assisted therapy and robotic pets, have gained attention, but their comparative effectiveness remains unclear. This study aimed to evaluate and rank different pet-assisted approaches for reducing depressive symptoms in older adults using network meta-analysis. Methods: We systematically searched PubMed, Embase, Web of Science, and the Cochrane Library up to August 2025 for randomized controlled trials involving adults aged 60 years or older with depression. The protocol was prospectively registered on INPLASY (INPLASY2025100023). Depression severity, assessed using validated scales, was synthesized using a frequentist random-effects network meta-analysis framework. Results: Twenty trials involving 1073 participants were included. Live animal-assisted therapy produced the greatest reduction in depressive symptoms versus passive control (SMD −2.04; 95% CI −3.03 to −1.04). Combining it with gait training (structured walking-based activity conducted with the animal) was associated with a reduction in depressive symptoms (SMD −4.82; 95% CI −6.69 to −2.95). Robotic pets showed a directionally beneficial but non-significant effect (SMD −1.21; 95% CI −2.79 to 0.38). Conclusions: Pet-assisted interventions are effective in reducing depressive symptoms among older adults. Live animal-assisted therapy, particularly when delivered in structured or combined formats, shows the greater benefit. Robotic pets may serve as a practical alternative when live animals cannot be implemented. Full article

Pea protein isolates (PPIs) from Pisum sativum have emerged as strategic ingredients at the interface of nutrition, sustainability, and functional food design. This review synthesizes advances linking isolation procedures with molecular structure and techno-functional performance. We compare alkaline extraction–isoelectric precipitation with wet and dry fractionation, as well as green/fermentation-assisted methods, highlighting the purity–functionality trade-offs driven by denaturation, aggregation, and the removal of anti-nutritional factors. We relate globulin composition (vicilin/legumin ratio), secondary/tertiary structure, and disulfide chemistry to interfacial activity, solubility, gelation thresholds, and long-term emulsion stability. Structure-guided engineering strategies are critically evaluated, including enzymatic hydrolysis, deamidation, transglutaminase cross-linking, ultrasound, high-pressure homogenization, pH shifting, cold plasma, and selected chemical/glycation approaches. Application case studies cover high-moisture texturization for meat analogues, emulsion and Pickering systems, fermented dairy alternatives, edible films, and bioactive peptide-oriented nutraceuticals. We identify bottlenecks—weak native gel networks, off-flavors, acidic pH performance, and batch variability—and outline process controls and synergistic modifications that close functionality gaps relative to animal proteins. Finally, we discuss sustainability and biorefinery opportunities that valorize soluble peptide streams alongside globulin-rich isolates. By integrating extraction, structure, and function, the review provides a roadmap for designing PPI with predictable, application-specific performance. Full article

Animal-assisted intervention is widely used in children and adolescents with neurodevelopmental disorders. The aim of this review is to understand the perceptions of parents of children with neurodevelopmental disorders, regarding the benefits of their children’s participation in animal-assisted intervention. Using the PRISMA methodology, a search was conducted in the Web of Science and ProQuest Central databases. The number of articles included in the review was 23 after applying the selection criteria. The results indicate that in all interventions carried out with children with neurodevelopmental disorders, parents perceive improvements in their children mainly at the physical, social and emotional levels. In addition, they identify positive aspects that influence family functioning. It can be concluded that these types of interventions, regardless of the type of animal or activity, are a beneficial tool when addressing different symptoms associated with neurodevelopmental disorders, having an impact not only on the child or adolescent, but also on parents or caregivers. Full article