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21 pages, 360 KB  
Article
In Vitro and In Situ Evaluation of White Mulberry (Morus alba) Pomace and Leaf: Fermentation Kinetics, Digestibility, and Potential as Alternative Ruminant Feed Sources
by Zekeriya Safa İnanç and Huzur Derya Arik
Fermentation 2025, 11(12), 692; https://doi.org/10.3390/fermentation11120692 (registering DOI) - 12 Dec 2025
Abstract
Mulberry (Morus alba) by-products represent underutilized feed resources with potential for ruminant nutrition. This study evaluated the rumen fermentation kinetics and rumen digestibility of dried mulberry pomace (MP) and leaf (ML) to determine optimal inclusion strategies in dairy cattle diets. Mulberry [...] Read more.
Mulberry (Morus alba) by-products represent underutilized feed resources with potential for ruminant nutrition. This study evaluated the rumen fermentation kinetics and rumen digestibility of dried mulberry pomace (MP) and leaf (ML) to determine optimal inclusion strategies in dairy cattle diets. Mulberry pomace (MP) and mulberry leaf (ML) were sun-dried and incorporated at 50% substitution levels into total mixed rations (TMR) with varying concentrations (30%, 35%, 40%, 45%, and 50%) of neutral detergent fiber (NDF). This created ten treatment groups: 30NP through 50NP (pomace-supplemented, where the number represents basal TMR NDF%) and 30NL through 50NL (leaf-supplemented), plus control groups containing only MP or ML and five basal TMR controls (30N through 50N). Rumen fluid was collected from two non-lactating Holstein cows fitted with ruminal cannulas. Chemical analysis revealed that ML contained 19% crude protein and 27.4% NDF, while MP contained 14.9% crude protein and 35.8% NDF. The highest gas production was observed in the 45NP (43.20 mL) and 50NL (43.50 mL) groups. Results demonstrated that MP achieved optimal fermentation when combined with 40–45% NDF TMR (maximum total volatile fatty acid (VFA): 88.86 mmol/L in 40NP at 48 h), whereas ML performed best with 45% NDF TMR (45NL: 88.03 mmol/L total VFA), indicating these as the most promising treatment combinations for ruminant feeding systems pending in vivo validation. Acetate proportions were higher in ML groups (84–96%), while propionate ratios were elevated in MP groups. Both materials maintained optimal ruminal pH (6.2–6.8). In vitro NDF digestibility was significantly higher for ML, with differences increasing from 2.97% at 2 h to 16.44% at 240 h. In situ degradation of MP was nearly complete at 48 h, while ML reached maximum degradation at 24 h. These findings indicate the potential of MP and ML as valuable alternative feed sources for ruminants, particularly in TMRs containing 40–45% NDF. Full article
(This article belongs to the Special Issue Ruminal Fermentation: 2nd Edition)
36 pages, 8096 KB  
Article
Enhancing Sheep Rumen Function, and Growth Performance Through Yeast Culture and Oxalic Acid Supplementation in a Hemicellulose-Based Diet
by Natnael D. Aschalew, Jialei Liu, Yuetong Liu, Wuwen Sun, Guopei Yin, Long Cheng, He Wang, Wanzhu Zhao, Longyu Zhang, Ziyuan Wang, Huaizhi Jiang, Tao Wang, Guixin Qin, Yuguo Zhen and Zhe Sun
Microorganisms 2025, 13(12), 2834; https://doi.org/10.3390/microorganisms13122834 - 12 Dec 2025
Abstract
Yeast culture (YC) is a microbial product that enhances ruminal fiber breakdown and improves nutrient digestion and utilization. Our previous research showed that oxalic acid (OA) is a crucial metabolite in YC that enhances rumen function. This study aimed to investigate the effects [...] Read more.
Yeast culture (YC) is a microbial product that enhances ruminal fiber breakdown and improves nutrient digestion and utilization. Our previous research showed that oxalic acid (OA) is a crucial metabolite in YC that enhances rumen function. This study aimed to investigate the effects of YC, OA, and their combination (YO) on rumen function, growth, and fattening in sheep. Twenty lambs were divided into 4 groups (ctrl, YC, OA, and YO; n = 5 each) and fed a diet supplemented with 2 levels of YC and 2 doses of OA for 60 days in a 2 × 2 factorial design. Growth and fattening performance, rumen microbiome analysis, serum indices and anti-oxidant levels, and metabolomic profiling were performed. Individual supplementation with YC and OA significantly increased the digestibility of dry matter (DM), organic matter (OM), and crude protein (CP) (p < 0.001); neutral detergent fiber (NDF) (p < 0.05); and acid detergent fiber (ADF) (p < 0.001) and their interaction significantly increased dry matter intake (DMI) (p = 0.05). Serum IgA and IgM levels were higher in the supplemented groups (p < 0.05). Serum calcium levels were higher in the OA and YO groups (p < 0.001). The supplemented groups showed significantly higher growth hormone and superoxide dismutase levels (p < 0.05). The longissimus dorsi muscle had higher levels of iron in the OA and YO groups; zinc in the OA, YO, and YC groups (p < 0.01); and selenium in the YC group (p < 0.05). The OA group had a higher total antioxidant capacity. All supplemented groups showed higher bacterial richness and diversity. Ruminococcus, Succinivibrio, and Fibrobacter were positively correlated with the fermentation and digestibility parameters. The supplementation also altered metabolite levels and types in key physiological pathways. In conclusion, this supplementation improved bacterial composition, nutrient digestibility, weight gain, carcass weight and quality, serum indices, antioxidant levels and metabolomic profiles. This suggests potential for the development of dietary supplements for ruminants. Full article
(This article belongs to the Special Issue Rumen Microorganisms)
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16 pages, 1102 KB  
Article
Associations of Lactoferrin-Fortified Formula with Infant Growth and Gut Microbiota: A Real-World Observational Study
by Xiaojin Shi, Biao Liu, Wenhui Ye, Xuanjing Qi, Menglu Xi, Shuqi Liu, Qihan Zhu, Lutong Zheng and Ai Zhao
Nutrients 2025, 17(24), 3896; https://doi.org/10.3390/nu17243896 - 12 Dec 2025
Abstract
Background/Objectives: Lactoferrin, a key bioactive component in human milk, may bridge functional gaps in infant formula; however, its long-term effects on growth and the gut microbiota in term infants remain underexplored, particularly in real-world settings. Methods: This real-world evidence (RWE) study assessed the [...] Read more.
Background/Objectives: Lactoferrin, a key bioactive component in human milk, may bridge functional gaps in infant formula; however, its long-term effects on growth and the gut microbiota in term infants remain underexplored, particularly in real-world settings. Methods: This real-world evidence (RWE) study assessed the impact of lactoferrin-fortified formula (LF) on infant growth, the gut microbiota, and feeding tolerance compared with control formula (CF) and exclusive breastfeeding (BF). After propensity score matching (PSM) for maternal education level and infant age, 111 matched Chinese infants (37 per group: LF, CF, and BF; age: 6–12 months) were analyzed. Growth was evaluated using WHO Z-scores (WAZ, LAZ, WLZ, and zBMI). The gut microbiota was profiled via 16S rRNA sequencing (n = 81). Feeding challenges were quantified using the Montreal Children’s Hospital Feeding Scale (MCH-FS). Results: The LF group exhibited significantly higher length-for-age Z-scores (LAZ) compared with both the BF and CF groups (p < 0.001), indicating superior linear growth. LF infants also showed reduced MCH-FS scores (18.0 vs. 36.2 in CF; p < 0.001), signifying fewer feeding difficulties. Gut microbiota analysis revealed enrichment of Bifidobacterium breve and butyrate-producing taxa (e.g., Faecalibacterium and Ruminococcaceae), higher alpha diversity, and metabolic divergence, involving enhanced lysine fermentation to acetate/butyrate in LF infants, suggesting a higher level of short-chain fatty acid (SCFA) production. Beta diversity analysis demonstrated that the LF microbiota clustered close to BF. Conclusions: Lactoferrin-fortified formula was associated with improved linear growth and feeding tolerance while shaping a healthy gut microbiota, showing similarities to breastfed infants’ microbiota. These findings support LF fortification as a strategy to improve functional outcomes in formula-fed infants. Full article
(This article belongs to the Section Pediatric Nutrition)
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24 pages, 1196 KB  
Article
Long-Term Straw Return Combined with Chemical Fertilizer Enhances Crop Yields in Wheat-Maize Rotation Systems by Improving Soil Nutrients Stoichiometry and Aggregate Stability in the Shajiang Black Soil (Vertisol) Region of North China Plain
by Xian Tang, Yangfan Qu, Yu Wu, Shasha Li, Fuwei Wang, Dongxue Li, Xiaoliang Li, Jianfei Wang and Jianrong Zhao
Agronomy 2025, 15(12), 2861; https://doi.org/10.3390/agronomy15122861 - 12 Dec 2025
Abstract
The sustainability of wheat-maize rotation systems in the North China Plain is challenged by the over-reliance on chemical fertilizers, which leads to the decline of soil organic matter and structural degradation, particularly in the unique Shajiang black soil (Vertisol). While straw return is [...] Read more.
The sustainability of wheat-maize rotation systems in the North China Plain is challenged by the over-reliance on chemical fertilizers, which leads to the decline of soil organic matter and structural degradation, particularly in the unique Shajiang black soil (Vertisol). While straw return is widely recommended to mitigate these issues, the synergistic mechanisms of its long-term combination with chemical fertilizers on soil nutrient stoichiometry and aggregate stability remain inadequately quantified. A long-term field experiment was conducted with the five fertilization treatments including: (1) no fertilizer or straw (CK), (2) chemical fertilizer alone (NPK), (3) straw return chemical fertilizer (NPKS), (4) straw return with 10% straw-decomposing microbial inoculant combined with chemical fertilizer (10%NPKS), and (5) straw return with 20% straw-decomposing microbial inoculant combined with chemical fertilizer (20%NPKS) in the Shajiang black soil (Vertisol) region to investigate the effects of straw return combined with chemical fertilizers on soil organic carbon (SOC), total nitrogen (TN) and total phosphorus (TP) stoichiometry, aggregate stability, and crop yield in winter wheat-summer maize rotation systems of North China Plain. Our study demonstrated that the co-application of straw with a straw-decomposing microbial inoculant is a highly effective strategy for enhancing soil health and crop productivity, with its efficacy being critically dose-dependent. Our results identified the 10%NPKS treatment as the optimal practice. It most effectively improved soil physical structure by significantly increasing the content of large macroaggregates (>0.5 mm) and key stability indices (MWD, GMD, WA), while concurrently enhancing nutrient cycling, as evidenced by elevated SOC, TN, and shifted C/P and N/P stoichiometry. Multivariate analyses confirmed strong positive correlations among these soil properties, indicating a synergistic improvement in soil quality. Crucially, these enhancements translated into significant yield gains, with a notable crop-specific response: maize yield was maximized under the 10%NPKS treatment, whereas wheat yield benefited sufficiently from NPKS treatment. A key mechanistic insight was that 20%NPKS treatment, despite leading to the highest SOC and TN, induced a relative phosphorus limitation and likely caused transient nutrient immobilization, thereby attenuating its benefits for soil structure and yield. We conclude that co-applying straw with a 10% microbial inoculant combined with chemical fertilizer represents the superior strategy, offering a sustainable pathway to synergistically improve soil structure, nutrient availability, and crop productivity, particularly in maize-dominated systems. Full article
(This article belongs to the Special Issue Plant Nutrition Eco-Physiology and Nutrient Management)
18 pages, 3866 KB  
Article
Silicon-Mediated Mitigation of Moderate Ammonium Stress in Maize Seedlings
by Hilário Júnior de Almeida, Anelisa de Aquino Vidal Lacerda Soares, Victor Manuel Vergara Carmona and Renato de Mello Prado
Plants 2025, 14(24), 3793; https://doi.org/10.3390/plants14243793 (registering DOI) - 12 Dec 2025
Abstract
Intensive irrigated agriculture relies heavily on nitrogen fertilization, which may cause ammonium accumulation, highly detrimental to sensitive seedlings. Silicon application has emerged as a potential strategy to mitigate this stress, although the underlying mechanisms remain poorly understood. To evaluate this effect, maize seedlings [...] Read more.
Intensive irrigated agriculture relies heavily on nitrogen fertilization, which may cause ammonium accumulation, highly detrimental to sensitive seedlings. Silicon application has emerged as a potential strategy to mitigate this stress, although the underlying mechanisms remain poorly understood. To evaluate this effect, maize seedlings were grown in nutrient solution under five N concentrations (1.4, 3.6, 7.1, 14.3, and 28.6 mmol L−1), applied in the presence or absence of silicon (1.8 mmol L−1 Si). The nitrogen source was a mixture of nitrate and ammonium in a N-NO3: N-NH4+ ratio of 4:5. Silicon was supplied as monosilicic acid (H2SiO3). Plant growth, leaf area, root morphology (length, diameter, density), N and Si accumulation, uptake and utilization efficiency, SPAD index, nitrate reductase activity, and proline content were evaluated. Silicon supplementation enhanced nitrate reductase activity, SPAD values, leaf area, and root traits, reduced proline in roots and shoots, and improved N uptake and partitioning. Among the tested N concentrations, 14.3 mmol L−1 achieved the highest efficiency of nutrient absorption and biomass production, highlighting silicon as a sustainable strategy to mitigate ammonium stress in maize seedlings. Full article
(This article belongs to the Special Issue Silicon and Its Physiological Role in Plant Growth and Development)
19 pages, 1280 KB  
Article
Optimization of Nitrogen Fertilizer Operation for Sustainable Production of Japonica Rice with Different Panicle Types in Liaohe Plain: Yield-Quality Synergy Mechanism and Agronomic Physiological Regulation
by Xinyi Lou, Meiling Li, Lin Zhang, Baoyan Jia, Shu Wang, Yan Wang, Yuancai Huang, Chanchan Zhou and Yun Wang
Sustainability 2025, 17(24), 11152; https://doi.org/10.3390/su172411152 - 12 Dec 2025
Abstract
Northern japonica rice holds a significant position in China’s food security. However, the traditional nitrogen fertilizer management model (nitrogen application rate > 225 kg/ha, base fertilizer proportion > 50%) has led to serious sustainability problems: the nitrogen utilization rate is only 25–30%, resulting [...] Read more.
Northern japonica rice holds a significant position in China’s food security. However, the traditional nitrogen fertilizer management model (nitrogen application rate > 225 kg/ha, base fertilizer proportion > 50%) has led to serious sustainability problems: the nitrogen utilization rate is only 25–30%, resulting in a large amount of fertilizer waste and economic losses. At the same time, it causes a decline in rice quality, manifested as a 15–20% increase in chalkiness and an 8–12% decrease in palatability value. It has also brought about environmental problems such as soil acidification and eutrophication of water bodies. As an important japonica rice production area, the Liaohe Plain has significant differences in the response of semi-upright and curved panicle varieties to nitrogen fertilizer. However, the agronomic physiological mechanism for the coordinated improvement of yield and quality of japonica rice with different panicle types is still unclear at present, which limits the sustainable development of rice production in this region. For this purpose, in this study, the typical semi-upright spike variety Shendao 47 and the curved spike variety Shendao 11 from the Liaohe Plain were used as materials, and five nitrogen fertilizer treatments were set up: N1, no nitrogen application; N2–N4, conventional nitrogen application rate of 165–225 kg/ha; and N5, and optimized nitrogen application rate of 195 kg/ha allocated in the proportion of 40% base fertilizer, 15% tillering fertilizer, 25% tillering fertilizer, 15% panicle fertilizer, and 5% grain fertilizer. The synergistic regulatory effect of nitrogen fertilizer management on yield and rice quality was systematically explored, and the key agronomic physiological mechanisms were analyzed. The research results show that: (1) The optimized nitrogen fertilizer treatment (N5) achieved a significant increase in yield while reducing the input of nitrogen fertilizer. The yields of Shendao 47 and Shendao 11 reached 10.71–11.82 t/ha and 9.50–10.62 t/ha, respectively, increasing by more than 35% compared with the treatment without nitrogen. (2) The N5 treatment simultaneously improved the processing quality (the whole polished rice rate increased by 4.11%) and the appearance quality (the chalkiness decreased by 63.8% to 77%). (3) The dry matter accumulation during the tillering stage (≥3.2 t/ha) and the net assimilation rate during the scion development stage (≥12 g/m2/d) were identified as key agronomic physiological indicators for regulating the yield-quality synergy. Optimizing nitrogen fertilizer management ensures an adequate supply of photosynthetic products through the high photosynthetic rate of flag-holding leaves and the extended lifespan of functional leaves. The phased nitrogen application strategy of “40% base fertilizer + 25% tillering fertilizer + 15% panicle fertilizer + 5% grain fertilizer” proposed in this study provides a theoretical and practical basis for the sustainable development of japonica rice production in the Liaohe Plain. This plan has achieved the coordinated realization of multiple goals including resource conservation (reducing nitrogen by 13%), environmental protection (lowering the risk of nitrogen loss), food security guarantee (stable increase in yield), and quality improvement (enhancement of rice quality), effectively promoting the development of the northern japonica rice industry towards a green, efficient and sustainable direction. Develop in the right direction. Full article
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16 pages, 3310 KB  
Article
Effects of Managed Disturbances on Plant Community Structure and Nutritional Function in the Tropical Savanna Habitat of the Endangered Eld’s Deer (Rucervus eldii)
by Jun Lan, Daogeng Yu, Yunnan Fu, Mingli Fu, Binbin He, Yu Guo, Xianbin Xing, Xuming Qi and An Hu
Agronomy 2025, 15(12), 2857; https://doi.org/10.3390/agronomy15122857 - 12 Dec 2025
Abstract
Managed disturbances and their consequences for plant community structure, productivity, and foliar nutrients in the habitat of the endangered Eld’s deer remain inadequately characterized. We assessed the effects of prescribed fire (PF), mechanical mowing (MM), and their combination (PF_MM) on plant communities in [...] Read more.
Managed disturbances and their consequences for plant community structure, productivity, and foliar nutrients in the habitat of the endangered Eld’s deer remain inadequately characterized. We assessed the effects of prescribed fire (PF), mechanical mowing (MM), and their combination (PF_MM) on plant communities in the Datian National Nature Reserve of Hainan, China. Our findings demonstrated that the PF_MM treatment produced the greatest number of species (38 species, representing increases of 26.6% and 72.7% compared to PF and MM, respectively) and diversity indexes, indicating enhanced structural stability relevant to ecological conservation. In contrast, MM yielded the highest aboveground biomass (AGB) and the highest foliar nitrogen (N, 14.28 g kg−1), phosphorus (P, 2.08 g kg−1), and potassium (K, 3.61 g kg−1) concentrations, but concurrently promoted shrub dominance, potentially risking long-term nutrient depletion and functional group imbalance. Legume (Fabaceae) richness was negatively associated with foliar P and K, which is consistent with the nutrient dilution effect often observed in more diverse plant communities. Structural equation modeling indicated that treatment effects on AGB were mediated by the importance value of Fabaceae, whereas treatment effects on foliar N and P were expressed both directly and indirectly via the richness of Fabaceae and other families. Consequently, no single management approach can simultaneously enhance all desired metrics or indices. New management strategies or technologies should be explored to balance biodiversity conservation with improved pasture quality, thereby further supporting the recovery of Eld’s deer habitat while maintaining ecosystem health. Full article
(This article belongs to the Section Grassland and Pasture Science)
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14 pages, 1787 KB  
Article
Clinical Evaluation of Autologous PRP (Platelet-Rich Plasma) in the Treatment of Periodontitis in Small-Breed Dogs
by Dmitrij Kvitka, Martinas Jankauskas, Matas Klupšas, Aistė Gradeckienė, Dalia Juodžentė and Greta Rudenkovaitė
Animals 2025, 15(24), 3581; https://doi.org/10.3390/ani15243581 - 12 Dec 2025
Abstract
Periodontal disease is a progressive inflammatory condition frequently diagnosed in dogs, particularly in small breeds such as Yorkshire Terriers, Toy Terriers, Spitz, Toy Poodles and other breeds predisposed to rapid plaque and tartar accumulation. As the field of regenerative medicine becomes more popular, [...] Read more.
Periodontal disease is a progressive inflammatory condition frequently diagnosed in dogs, particularly in small breeds such as Yorkshire Terriers, Toy Terriers, Spitz, Toy Poodles and other breeds predisposed to rapid plaque and tartar accumulation. As the field of regenerative medicine becomes more popular, more and more attention is being paid to substances that promote tissue regeneration, one of which is platelet-rich plasma (PRP). PRP is an autologous blood-derived product rich in growth factors that stimulate tissue regeneration and modulate inflammation. This study aimed to evaluate the clinical effectiveness of PRP injections without additional activating agents in the management of stage 2–3 periodontitis in small-breed dogs. Forty-two adult dogs (Yorkshire Terriers, Toy Terriers, Pomeranians, Toy Poodles, and Havanese) were enrolled and divided into two groups: PRP (n = 30) and control (n = 12). Following standard dental prophylaxis, the PRP group received gingival, submucosal, and periodontal pocket injections of PRP (0.1 mL per site). Periodontitis stage, gingival index, periodontal pocket depth, and horizontal bone loss were evaluated at baseline and 30 days post-treatment. PRP therapy significantly improved all evaluated parameters (p < 0.05). The gingival index decreased threefold, periodontal pocket depth was reduced twofold, and horizontal bone loss decreased by more than twofold compared with baseline and controls. No adverse reactions, discomfort, or postoperative complications were observed. The administration of non-activated PRP as an adjunct to dental cleaning significantly enhances soft and hard tissue regeneration in small-breed dogs with stage 2–3 periodontitis. PRP therapy represents a safe, minimally invasive, and effective regenerative approach for improving periodontal health in routine veterinary dentistry. Full article
(This article belongs to the Section Veterinary Clinical Studies)
13 pages, 1237 KB  
Article
Sustainable Solid Biofuel Production from Coffee Byproducts: Characterization and Pelletization of Husk and Parchment
by Shoraia Germani Winter, Antonio José Vinha Zanuncio, Amélia Guimarães Carvalho, Marcos Vinícius Ferreira, Olivia Pereira Lopes, Angélica de Cassia Oliveira Carneiro and Solange de Oliveira Araújo
Resources 2025, 14(12), 188; https://doi.org/10.3390/resources14120188 - 12 Dec 2025
Abstract
Brazil is the world’s largest coffee producer, resulting in the production of 1 kg of husk and 0.5 kg of parchment for every 1 kg of coffee beans. Given the large amount of biomass and the constant need for energy production, this study [...] Read more.
Brazil is the world’s largest coffee producer, resulting in the production of 1 kg of husk and 0.5 kg of parchment for every 1 kg of coffee beans. Given the large amount of biomass and the constant need for energy production, this study raises the possibility of using waste for pellet production. Samples of coffee husks and parchment were characterized by moisture content (dry basis), proximate analysis (volatile matter, ash and fixed carbon), calorific value, elemental analysis, and thermogravimetry, and the pellets were characterized by moisture content (dry basis), bulk density, energy density, mechanical durability, percentage of fines, and hardness. The results were compared with the ISO 17225-6. The parchment had a higher carbon, 49.5%, C/N 45.1%, and lignin 26.2% and lower ashes 2.8% and extractives 14.2%, resulting in higher calorific value, while coffee husks obtained 46.5%, 26.3%, 24.6%, 5.5%, and 34.3%, respectively. Pellets produced with parchment had a higher density 622 kg/m3 and lower moisture content 10.5%, resulting in higher energy density. The parchment pellets met all the parameters of the ISO 17225-6, while the coffee husk pellets did not meet the parameters for moisture, which is less than 15%, and bulk density, greather than 600 kg/m3. Both types of biomass showed potential for pellet production, with further studies needed on coffee husks. Full article
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21 pages, 904 KB  
Review
Prenatal Exposure to Tobacco Smoke and Vaping Aerosols: Mechanisms Disrupting White-Matter Formation
by Sebastián Beltran-Castillo, Juan Pablo Espinoza and Michelle Grambs
Toxics 2025, 13(12), 1071; https://doi.org/10.3390/toxics13121071 - 12 Dec 2025
Abstract
White-matter development during fetal life represents one of the most vulnerable processes to environmental disruption, as it relies on the precisely timed proliferation, migration, and differentiation of oligodendrocyte lineage cells. Among environmental threats, exposure to toxic compounds contained in tobacco smoke and vaping [...] Read more.
White-matter development during fetal life represents one of the most vulnerable processes to environmental disruption, as it relies on the precisely timed proliferation, migration, and differentiation of oligodendrocyte lineage cells. Among environmental threats, exposure to toxic compounds contained in tobacco smoke and vaping aerosols represents a major yet preventable risk during pregnancy. Despite growing awareness, tobacco smoking remains widespread, and a substantial proportion of the population—including pregnant women—continues to perceive electronic nicotine delivery systems (ENDS) as less harmful, a misconception that contributes to persistent prenatal exposure. These products expose the fetus to numerous substances that readily cross the placenta and reach the developing brain, including compounds with endocrine-disrupting activity, where they interfere with white-matter development. Epidemiological and neuroimaging studies consistently reveal microstructural alterations in white matter that correlate with long-term cognitive and behavioral impairments in offspring exposed in utero. These alterations may arise from both nicotine-specific pathways and the actions of other toxicants in cigarette smoke and ENDS aerosols that cross the placenta and disrupt white-matter emergence and maturation. Preclinical research provides mechanistic insight: nicotine acts directly on nicotinic acetylcholine receptors (nAChRs) in oligodendrocyte precursor cells, disrupting calcium signaling and differentiation, while additional constituents of smoke and vaping aerosols also affect astrocyte and microglial function and disturb the extracellular milieu required for proper myelination. Full article
(This article belongs to the Special Issue Reproductive and Developmental Toxicity of Environmental Factors)
21 pages, 2046 KB  
Article
Mitigation of Greenhouse Gas Emissions Through Straw Management and Oxygenated and Biochar-Based Fertilizers
by Qi Sun, Yu-Feng Wang, Hao Jiang, Huichang Bian, Xiao-Jun Wang, Yan Li, Hong-Sheng Gao, Xue Pan, Shuai Hao and Xue-Jia Gu
Plants 2025, 14(24), 3791; https://doi.org/10.3390/plants14243791 - 12 Dec 2025
Abstract
Straw returning is a common agricultural practice that can enhance rice (Oryza sativa L.) yield in paddy systems. However, it also leads to a significant increase in greenhouse gas emissions (GHG). Fortunately, this negative impact can be mitigated by implementing enhanced oxygenation [...] Read more.
Straw returning is a common agricultural practice that can enhance rice (Oryza sativa L.) yield in paddy systems. However, it also leads to a significant increase in greenhouse gas emissions (GHG). Fortunately, this negative impact can be mitigated by implementing enhanced oxygenation strategies during rice cultivation. This study explored the effects of various oxygenation measures on GHG under straw-returning conditions through controlled pot experiments. Six distinct treatments were applied. These included straw not returned (NR, no straw applied), straw returned (SR), controlled irrigation (CI), oxygenation irrigation (OI), application of oxygenated fertilizer (OF, CaO2), and use of biochar-based fertilizer (CF). All treatment groups, with the exception of the NR group, involved the return of straw to the field. Creating rice production methods that increase yield and decrease emissions is of great importance to agricultural ecology. We postulated that using aeration methods under straw return conditions would stabilize rice yield and reduce GHG. The experimental results were consistent with our hypothesis. The experiment evaluated multiple parameters, including rice yield, leaf photosynthetic performance, soil ammonium and nitrate nitrogen (N) levels, and greenhouse gas emissions. The findings revealed that different oxygenation approaches significantly promoted rice tillering. Oxygenation measures have been shown to enhance rice yield by 19% to 65%. The highest tiller numbers were observed in the SR (22.75) and CF (21.6) treatments. Among all treatments, the CF achieved the highest seed setting rate at 0.94, which was notably greater than that of the other treatments. Total plant biomass was also significantly higher in the straw returning treatment (109.36 g), surpassing all other treatments. In terms of soil nitrogen dynamics, the OF treatment resulted in the highest nitrate nitrogen content. Meanwhile, the ammonium nitrogen concentrations across the four oxygenation treatments (CI, OI, OF, CF) ranged from approximately 7 to 8.9 mg kg−1. Regarding GHG, the CF treatment exhibited the lowest methane emissions, which were 33% lower compared to the straw returning treatment. The OF led to a 22% reduction in carbon dioxide emissions (CO2) relative to straw returning. Most notably, the CF reduced nitrous oxide emissions by 37% compared to the straw returning treatment. Overall, SR was found to substantially increase GHG. In contrast, all tested oxygenation measures—CI, OI, OF, and CF—were effective in suppressing GHG to varying degrees. Among these, the CF and OF demonstrated the most balanced and outstanding effects, both in reducing emissions and maintaining stable rice yields. Full article
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26 pages, 646 KB  
Article
Development and Consumer Acceptability of Functional Bread Formulations Enriched with Extruded Avocado Seed Flour: Nutritional and Technological Properties
by Jesús Salvador Jaramillo-De la Garza, Dariana Graciela Rodríguez-Sánchez, Carmen Hernández-Brenes and Erick Heredia-Olea
Foods 2025, 14(24), 4282; https://doi.org/10.3390/foods14244282 - 12 Dec 2025
Abstract
Avocado processing generates seed by-products rich in dietary fiber that can be upcycled into functional ingredients. This study modified and characterized avocado seed flour via extrusion and enzyme-assisted wet-milling, as well as evaluated its use in wheat bread. The flour was fractionated, and [...] Read more.
Avocado processing generates seed by-products rich in dietary fiber that can be upcycled into functional ingredients. This study modified and characterized avocado seed flour via extrusion and enzyme-assisted wet-milling, as well as evaluated its use in wheat bread. The flour was fractionated, and fraction 2 (F2) was selected based on techno-functional performance; it was tested in its non-extruded (NEF2) and extruded (EF2) forms. Breads were prepared by replacing 5% of wheat flour with NEF2 and EF2 (NEB and EB, respectively). Compared with NEF2, EF2 had an 81% higher water absorption index (WAI) and an 18% higher oil absorption index (OAI). Extrusion reduced antioxidant activity ~1.6-fold, consistent with an ~85% decrease in acetogenin content, indicating thermo-mechanical degradation of bioactives linked to bitterness. Analyses were conducted in triplicate (p < 0.05). By day 3, crumb hardness increased (EB: 9.65 N; NEB: 6.04 N; control: 5.49 N). In a test with 106 consumers, aroma scores improved for NEB (8.00, IQR 7.00–8.00) and EB (7.00, IQR 5.00–8.00) versus the control (6.00, IQR 4.00–7.00), while overall acceptability, texture, color, and appearance did not differ. These results support EF2 as a functional upcycled ingredient that enhances hydration and aroma, reduces bitterness, and maintains consumer acceptance, aligning with circular economy and clean-label goals. Full article
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11 pages, 2591 KB  
Article
Synthesis of Novel Anion Recognition Molecules as Quinazoline Precursors
by Gábor Krajsovszky, László Piros, Dóra Bogdán, Eszter Kalydi, Tamás Gáti, Pál Szabó, Péter Horváth and István M. Mándity
Int. J. Mol. Sci. 2025, 26(24), 11975; https://doi.org/10.3390/ijms262411975 - 12 Dec 2025
Abstract
Thiourea and structurally related urea derivatives are widely recognised for their ability to transport anions through hydrogen bonding interactions. The strength of these interactions correlates with the electronegativity of the ligand and the acidity of the NH hydrogens involved. Thiourea, being more acidic [...] Read more.
Thiourea and structurally related urea derivatives are widely recognised for their ability to transport anions through hydrogen bonding interactions. The strength of these interactions correlates with the electronegativity of the ligand and the acidity of the NH hydrogens involved. Thiourea, being more acidic than urea, exhibits partial deprotonation in the presence of certain anions such as organic carboxylates, fluoride, and bromide, while remaining resistant to deprotonation by chloride. This behaviour suggests a degree of selectivity toward chloride ions. Additionally, while carbamide-containing molecules tend to aggregate—potentially reducing their ion-binding efficiency—thiourea derivatives show reduced aggregation, preserving their binding capabilities. In this study, we report the synthesis and characterisation of 21 novel thiourea derivatives obtained by reacting 2-aminobenzoylamino acid esters with various substituted phenyl isothiocyanates. Seven similar thiourea-containing molecules were made as a comparison—without the amino acids—by reacting aniline with the different phenyl isothiocyanates. The reaction kinetics were found to be influenced primarily by the electronic nature of the substituents on the phenyl ring. Electron-withdrawing groups (EWGs), such as para-nitro, 3,5-bis(trifluoromethyl), and fluorine, accelerated the reaction, while electron-donating groups (EDGs), such as para-methoxy, slowed it down. Interestingly, the nature of the amino acid precursors had no significant impact on reaction time; however, reactions with aniline proceeded the fastest. Solvent choice also played a role: reactions in N,N-dimethylformamide (DMF) proceeded faster than in acetone, although with reduced yields. Consequently, reaction conditions were optimised to balance time efficiency and product yield. To evaluate the chloride ion-binding properties of the synthesised compounds, 1H NMR titration experiments were conducted in deuterated dimethyl sulfoxide (DMSO-d6). The association constants (Ka) derived from these studies revealed a clear correlation with the electronic nature of the substituents. Compounds bearing EWGs exhibited enhanced chloride binding, while those with EDGs showed diminished binding affinity. Surprisingly, the presence of amino acid moieties led to a decrease in Ka values, despite the electron-withdrawing nature of the amide groups. This suggests that steric or conformational factors may play a role in modulating binding strength. Overall, the synthesised thiourea derivatives demonstrate mild, reversible chloride ion-binding behaviour, making them promising candidates for further development as selective anion receptors. The insights gained from this study contribute to a deeper understanding of structure–activity relationships in anion-binding systems and may inform the design of future supramolecular architectures with tailored ion recognition properties. Full article
(This article belongs to the Special Issue Techniques and Strategies in Drug Design and Discovery, 3rd Edition)
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25 pages, 1800 KB  
Article
Assessing Water and Nitrogen Management Practices in Drip-Irrigated Desert Lettuce
by Aliasghar Montazar, Daniel Geisseler and Michael D. Cahn
Horticulturae 2025, 11(12), 1507; https://doi.org/10.3390/horticulturae11121507 - 12 Dec 2025
Abstract
Efficient water and nitrogen (N) management is critical for sustainable lettuce production in arid regions. This study evaluated N and water management practices for drip-irrigated desert lettuce over three growing seasons at the University of California Desert Research and Extension Center. Two irrigation [...] Read more.
Efficient water and nitrogen (N) management is critical for sustainable lettuce production in arid regions. This study evaluated N and water management practices for drip-irrigated desert lettuce over three growing seasons at the University of California Desert Research and Extension Center. Two irrigation levels and three N application rates were tested in 1 m and 2 m wide bed configurations. The CropManage (CM) decision support tool was used to estimate crop water requirements for a 100% evapotranspiration (ET) irrigation treatment and to determine the 100% N rate, with additional treatments at 125% ET and 80–120% of the CM-recommended N rate. CM was also used to verify water and N applications in eight commercial fields. Water levels, N rates, and their interaction had no significant effect on yields, whereas 2 m wide beds produced significantly higher yields than 1 m wide beds (p < 0.05). Higher N rates increased N uptake at harvest and significantly reduced N use efficiency (NUE), while higher water levels reduced water use efficiency (WUE) (p < 0.05). Lettuce N uptake increased linearly from thinning to harvest at an average rate of 2.9 kg ha−1 d−1. Across commercial sites, CM-recommended applications were lower than farmer standard practice, reducing irrigation by 34% (from 364 mm to 239 mm) and N inputs by 29% (from 202 kg ha−1 to 144 kg ha−1). These findings provide refined N-uptake estimates and highlight opportunities to optimize water and N management, positioning CM as a practical decision-support tool for desert lettuce production. Full article
(This article belongs to the Section Vegetable Production Systems)
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22 pages, 12895 KB  
Article
The Role of Rotational Tool Speed in the Joint Performance of AA2024-T4 Friction Stir Spot Welds at a Short 3-Second Dwell Time
by Yousef G. Y. Elshaghoul, Mahmoud F. Y. Shalaby, Mohamed M. El-Sayed Seleman, Ahmed Elkelity, Hagar A. Reyad and Sabbah Ataya
Crystals 2025, 15(12), 1054; https://doi.org/10.3390/cryst15121054 - 12 Dec 2025
Abstract
This study explores Friction Stir Spot Welding (FSSW), a well-established solid-state joining technique, for high-strength aluminum alloys like AA2024-T4, which present significant challenges for conventional welding techniques. This research focuses on the impact of relatively low rotational speeds, specifically within a range of [...] Read more.
This study explores Friction Stir Spot Welding (FSSW), a well-established solid-state joining technique, for high-strength aluminum alloys like AA2024-T4, which present significant challenges for conventional welding techniques. This research focuses on the impact of relatively low rotational speeds, specifically within a range of 700 to 1300 rpm, on the mechanical and microstructural properties of the welded joints. By employing a short dwell time of 3 s, this study aims to enhance productivity in the automotive and aerospace industries. The experimental work evaluated the joints’ thermal cycles, macrostructure, microstructure, hardness and load-carrying capacity. Results indicated a linear relationship between rotational speed and heat input. Although all welds exhibited a significant grain size reduction in the stir zone (SZ) compared to the base material (29.7 ± 6.1 μm), the SZ grain size increased with rotational speed, ranging from 4.7 ± 1.4 to 8.3 ± 1.3 μm. This study identified 900 rpm as the optimal parameter, achieving the highest load-carrying capacity (7.35 ± 0.4 kN) and a high SZ hardness (99 ± 1.5 HV). These findings confirm that joint strength is a balance between grain refinement and thermal softening. The presence of precipitates and the fractography of the tensile–shear tested specimens were also investigated and discussed. Full article
(This article belongs to the Special Issue Development of Light Alloys and Their Applications)
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