Beneficial Effects of Galectin-3 Blockade in Vascular and Aortic Valve Alterations in an Experimental Pressure Overload Model
Abstract
:1. Introduction
2. Results
2.1. Aortic Structural Changes Are Improved by Galectin-3 (Gal-3) Blockade in Pressure Overload Rats
2.2. Gal-3 Pharmacological Blockade Diminishes Aortic Remodeling in Pressure Overload (PO) Rats
2.3. Inhibition of Gal-3 Attenuates Vascular Inflammation in PO Rats
2.4. Gal-3 Blockade Reduces Aortic Valve (AV) Thickening and Fibrosis in PO Rats
2.5. Gal-3 Inhibition Decreases AV Inflammation and Calcification in PO Rats
3. Discussion
4. Materials and Methods
4.1. Animals
4.2. Histological Analysis
4.3. Real-Time Reverse Transcription Polymerase Chain Reaction (PCR)
4.4. ELISA
4.5. Gelatin Zymography
4.6. Statistical Analysis
5. Conclusions
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Ibarrola, J.; Martínez-Martínez, E.; Sádaba, J.R.; Arrieta, V.; García-Peña, A.; Álvarez, V.; Fernández-Celis, A.; Gainza, A.; Rossignol, P.; Cachofeiro Ramos, V.; et al. Beneficial Effects of Galectin-3 Blockade in Vascular and Aortic Valve Alterations in an Experimental Pressure Overload Model. Int. J. Mol. Sci. 2017, 18, 1664. https://doi.org/10.3390/ijms18081664
Ibarrola J, Martínez-Martínez E, Sádaba JR, Arrieta V, García-Peña A, Álvarez V, Fernández-Celis A, Gainza A, Rossignol P, Cachofeiro Ramos V, et al. Beneficial Effects of Galectin-3 Blockade in Vascular and Aortic Valve Alterations in an Experimental Pressure Overload Model. International Journal of Molecular Sciences. 2017; 18(8):1664. https://doi.org/10.3390/ijms18081664
Chicago/Turabian StyleIbarrola, Jaime, Ernesto Martínez-Martínez, J. Rafael Sádaba, Vanessa Arrieta, Amaia García-Peña, Virginia Álvarez, Amaya Fernández-Celis, Alicia Gainza, Patrick Rossignol, Victoria Cachofeiro Ramos, and et al. 2017. "Beneficial Effects of Galectin-3 Blockade in Vascular and Aortic Valve Alterations in an Experimental Pressure Overload Model" International Journal of Molecular Sciences 18, no. 8: 1664. https://doi.org/10.3390/ijms18081664