The Development of Recombinant and Subunit Vaccines Against Infectious Diseases

A special issue of Vaccines (ISSN 2076-393X). This special issue belongs to the section "Vaccines against Infectious Diseases".

Deadline for manuscript submissions: closed (31 October 2025) | Viewed by 1233

Special Issue Editor


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Guest Editor
Department of Microbiology, College of Medicine, Health Sciences Centre, Kuwait University, Kuwait City, Kuwait
Interests: infectious diseases; recombinant vaccines; adjuvants; delivery systems
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Special Issue Information

Dear Colleagues,

While significant progress has been made in preventing and controlling infectious diseases globally, humanity continues to suffer from their devastating effects. Vaccines play a crucial role in combating infectious diseases. Many successful vaccines have been developed to immunize against human infectious diseases, thereby reducing morbidity and mortality. Nevertheless, there remains a need for improved or novel vaccines to provide optimal and long-lasting protection against various infectious diseases. One strategy for developing new and more effective vaccines involves using immunogenic proteins in the form of recombinant vaccines. However, recombinant proteins are generally weak antigens and necessitate appropriate adjuvants and delivery systems to enhance their protective efficacy. Incorporating adjuvants can reduce the antigens required to elicit an optimal immune response, thereby minimizing adverse effects at the inoculation site.

This Special Issue invites original research and review articles on the efficacy of adjuvanted recombinant vaccines in preclinical and clinical trials against bacterial, parasitic, fungal, and viral infections for consideration.

Prof. Dr. Abu Salim Mustafa
Guest Editor

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Keywords

  • infectious diseases
  • vaccines
  • recombinant antigens
  • adjuvants

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Published Papers (1 paper)

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Research

17 pages, 3216 KB  
Article
The DNA Vaccines for the Gn and Gc Heterologous Polymer of Severe Fever with Thrombocytopenia Syndrome Virus Induce Potent Immunogenicity in Mice
by Qiuju He, Xiaojuan Liu, Jincheng Tong, Huan Li, Heng Zhang, Jiamin Chen, Mengyi Zhang, Zhihua Li and Qianqian Li
Vaccines 2025, 13(12), 1186; https://doi.org/10.3390/vaccines13121186 - 24 Nov 2025
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Abstract
Introduction/Background: Severe fever with thrombocytopenia syndrome virus (SFTSV) poses a threat to global public health with a mortality rate of up to 30%. However, there is currently no commercialized SFTSV vaccine. This study focused on the construction of DNA vaccines with different structures [...] Read more.
Introduction/Background: Severe fever with thrombocytopenia syndrome virus (SFTSV) poses a threat to global public health with a mortality rate of up to 30%. However, there is currently no commercialized SFTSV vaccine. This study focused on the construction of DNA vaccines with different structures based on the surface glycoproteins Gn and Gc to identify the immunodominant conformations. Methods: The DNA vaccines encoding secretory proteins including Gn or Gc monomer, heterodimer of Gn and Gc (dimer), two forms of hexamer composed of the Gn and Gc heterodimer (hexamer-1 and hexamer-2) or ferritin nanoparticles of Gn, and non-secretory proteins including Gn (Gn-TM) and Gc (Gc-TM) were constructed. Western blot confirmed the expression level and the specificity of those DNA vaccines. After vaccinating mice with those DNA vaccines, its induced humoral and cellular immunity were comprehensively evaluated. Results: The DNA vaccines were constructed successfully. The DNA vaccines of Gn and polymers including dimer, hexamer-2, and ferritin nanoparticles inducing stronger binding antibody, neutralizing antibody, and antibody-dependent cellular cytotoxicity (ADCC) activity. The neutralizing antibody induced by these constructs was also cross-recognized by other five SFTSV pseudovirus strains. However, the T cell response induced by Gc, dimer or hexamer-2 DNA vaccines were significantly higher than those in most other groups, including Gn. Conclusion: The DNA vaccines encoding dimer or hexamer-2 demonstrated superior immunogenicity over other conformations, after taking the results of humoral and cellular responses into account. This study revealed the advantages of using polymer conformations in SFTSV vaccine design and provided new targets in SFTSV vaccine development. Full article
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