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Plants
Special Issues
Emerging Extraction Techniques for Bioactive Compounds Derived from Plant Resources
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Emerging Extraction Techniques for Bioactive Compounds Derived from Plant Resources

A special issue of Plants (ISSN 2223-7747). This special issue belongs to the section "Phytochemistry".

Deadline for manuscript submissions: 30 November 2025 | Viewed by 478

Special Issue Editors

Dr. Branimir Pavlic

E-Mail Website
Guest Editor
Faculty of Technology Novi Sad, University of Novi Sad, Bulevar cara Lazara 1, 21000 Novi Sad, Serbia
Interests: process engineering; statistical modeling; antioxidant activity; natural product chemistry; extraction; food chemistry
Special Issues, Collections and Topics in MDPI journals
Dr. Milica Aćimović

E-Mail Website
Guest Editor
Institute of Field and Vegetable Crops Novi Sad, National Institute of the Republic of Serbia, 21000 Novi Sad, Serbia
Interests: medicinal plants; growing technology; postharvest processing; biodiversity; ethnopharmacology; essential oil; hydrolate; climate changes
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

The decline in the use of synthetic compounds, chemicals, and additives, due to their toxic effects on humans and the environment, has shifted attention toward natural extracts obtained through sustainable and eco-friendly methods. High-pressure technologies and bio-based solvents have both emerged as critical categories of extraction techniques, addressing key challenges in green chemistry. These include reducing energy, time, and resource consumption while maintaining high yields and the quality of the extracts.

This Special Issue is focused on the utilization of emerging extraction techniques (high-pressure processes, such as supercritical fluid extraction, pressurized liquid extraction, and subcritical water extraction, ultrasounds, microwaves, pulsed electric fields, enzymes, and natural deep eutectic solvents) for recovering a diverse range of natural bioactive compounds, including polyphenols, terpenoids, carotenoids, and lipids. These compounds are commonly sourced from plant- and animal-based resources, as well as from under-utilized side streams such as wild-grown medicinal plants, by-products of the food industry, and agricultural waste streams.

This Special Issue welcomes high-quality research contributions that explore innovative approaches to extracting bioactive compounds using green extraction processes and their novel applications in various products. Topics of interest include process optimization, chemical characterization, and the evaluation of bioactive potential. Particular emphasis is placed on the integration of these extracts into food products, either as natural additives or in the creation of functional foods.

Dr. Branimir Pavlic
Dr. Milica Aćimović
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Plants is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • bioactive compounds
  • emerging extraction techniques
  • natural deep eutectic solvents
  • high-pressure processes
  • functional foods

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Published Papers (1 paper)

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29 pages, 2069 KiB  
Article
Extraction of Bioactive Compounds from Larrea cuneifolia Cav. Using Natural Deep Eutectic Solvents: A Contribution to the Plant Green Extract Validation of Its Pharmacological Potential
by Agostina Conta, Mario Juan Simirgiotis, José Martínez Chamás, María Inés Isla and Iris Catiana Zampini
Plants 2025, 14(7), 1016; https://doi.org/10.3390/plants14071016 - 24 Mar 2025
Viewed by 361
Abstract
A sustainable alternative to replace the use of toxic and non-biodegradable conventional solvents for the extraction of active principles from plants is natural deep eutectic solvents (NADESs). Larrea cuneifolia Cav. (Zygophyllaceae) is a plant widely distributed in semiarid areas of western Argentina. Several [...] Read more.
A sustainable alternative to replace the use of toxic and non-biodegradable conventional solvents for the extraction of active principles from plants is natural deep eutectic solvents (NADESs). Larrea cuneifolia Cav. (Zygophyllaceae) is a plant widely distributed in semiarid areas of western Argentina. Several studies validate its popular medicinal use by demonstrating its biological activities such as antibacterial, antifungal, antioxidant, anti-inflammatory, and anticarcinogenic properties, among others. The aim of this work was to compare the bioactive compounds and the in vitro antioxidant and antibacterial activity of L. cuneifolia extracts using non-conventional vs. conventional solvents. Aqueous, ethanolic, and four NADES extracts were prepared. The extracts were phytochemically characterized, and extracted compounds were identified by UHPLC-MS/MS. Antioxidant activity was determined by evaluating the hydrogen peroxide and free radical scavenging capacity using ABTS•+. The antibacterial activity of the extracts and NADESs was evaluated against Gram-positive and Gram-negative multidrug-resistant strains. The extracts of L. cuneifolia presented a variable content of total phenolic compounds between 4163.4 and 24,371.63 µg GAE/mL. Phenolic acids, flavonoid glycosides, flavanones, flavones, flavonols, alkaloids, lignans (nordihydroguaiaretic acid and its derivatives), and other compounds were tentatively identified in extracts of L. cuneifolia obtained with conventional and non-conventional solvents. A heatmap cluster and a bubble plot were created to compare the diversity and relative abundance of identified compounds, and the extracts were classified into two major groups. All extracts were able to scavenge > 40% of hydrogen peroxide and the ABTS radical cation (ABTS•+) (CD50 = 3.15–5.13 µg GAE/mL). The LAS extract exhibited the highest bacterial growth inhibition (MIC = 75–37.5 µg GAE/mL). In conclusion, the results show that NADESs represent a sustainable alternative for the extraction of compounds with antioxidant and antibacterial activity and could therefore replace traditional solvents in the pharmaceutical, cosmetic, or food industries. Full article
(This article belongs to the Special Issue Emerging Extraction Techniques for Bioactive Compounds Derived from Plant Resources)
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