Exploring the Formation of Adventitious Roots in Perennial Species: Insights and Perspectives

A special issue of Plants (ISSN 2223-7747). This special issue belongs to the section "Plant Development and Morphogenesis".

Deadline for manuscript submissions: 31 December 2025 | Viewed by 858

Special Issue Editors


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Guest Editor
Laboratorio de Biotecnología Aplicada y Genómica Funcional, Instituto de Botánica del Nordeste (IBONE-CONICET), Facultad de Ciencias Agrarias, Universidad Nacional del Nordeste, Sgto. Cabral 2131, Corrientes W3402BKG, Argentina
Interests: plant physiology; plant biotechnology; plant biology; plant breeding; abiotic stress tolerance; plant molecular biology; plant environmental stress physiology
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Guest Editor
Misión Biológica de Galicia (CSIC), Pontevedra, Spain
Interests: adventitious rooting; micropropagation; woody plants; transcriptomics; plant regeneration; gene expression
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Selecting perennial species for forestry, fruit, ornamental, or industrial purposes requires a comprehensive observation from the juvenile to adulthood stage. Upon identifying genotypes with desirable traits, implementing an effective vegetative propagation procedure becomes imperative to ensure the large-scale production of clonal plants. New plants should possess a profuse, high-quality adventitious root system to facilitate their rapid establishment in field conditions, endure the challenging circumstances of the canopy, and support rapid growth. In recent years, considerable progress has been made in elucidating the molecular complex that controls the development of adventitious roots in stem cuttings and shoot apices. At the same time, a better understanding of how the transition from the vegetative to the adult phase influences the morphogenic process has been achieved. Considering all these advancements, further research is needed to clarify the mechanisms that control the number of roots per cutting, the root structure, and the growth rate of the propagated plants. Integrating this information will yield valuable insights into the process and guide the development of strategies to enhance the quantity and quality of roots, thereby ensuring the fast establishment of the plantation. To improve our understanding of the factors impeding the mass propagation of perennial species, we invite the scientific community to contribute short notes, original articles, or reviews covering all aspects of adventitious rooting and growth of rooted cuttings, including reinvigoration and rejuvenation.

Dr. Pedro Alfonso Sansberro
Dr. Conchi Sánchez
Guest Editors

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Keywords

  • adventitious rooting
  • plant rejuvenation
  • stem cuttings
  • perennial species

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Published Papers (1 paper)

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Research

20 pages, 5175 KiB  
Article
Rejuvenation of Mature Ilex paraguariensis Plants Through Serial Rooted Cuttings: Exploring the Roles of miRNAs in Reversing Adult Phase, Promoting Root Formation, and Determining Root Structure
by María J. Duarte, Raúl M. Acevedo, Nicolás L. Ortiz, Mayra Y. Álvarez and Pedro A. Sansberro
Plants 2025, 14(11), 1668; https://doi.org/10.3390/plants14111668 - 30 May 2025
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Abstract
In plants, the transition from the juvenile to adult stage involves physiological and anatomical changes initiated and partially controlled by evolutionarily conserved microRNAs. This process is of particular significance for the successful propagation of woody plant species that have transitioned to vegetative maturity [...] Read more.
In plants, the transition from the juvenile to adult stage involves physiological and anatomical changes initiated and partially controlled by evolutionarily conserved microRNAs. This process is of particular significance for the successful propagation of woody plant species that have transitioned to vegetative maturity and are recalcitrant to propagation. Conserved miRNAs differentially expressed between rejuvenated and mature Ilex paraguariensis plants were identified using high-throughput sequencing of small RNA libraries. The expression of miR156/miR157/miR528 was high in the leaves of juvenile plants and gradually decreased as the plant transitioned from juvenile to adult stages. In contrast, miR172 was predominantly expressed in adult plants. This variation confirmed that adults transitioned back to a juvenile phase after serial-rooted cuttings, allowing the plants to regain juvenile characteristics. Rejuvenation promotes the formation of adventitious roots and improves root structure, which supports the overall growth of the plant and results in greater vigour. The results will offer insights for further investigation into the molecular mechanisms regulating vegetative phase change in I. paraguariensis and other recalcitrant woody plant species. This knowledge could facilitate the earlier identification of rejuvenated material by analysing a wider range of genotypes and maturation stages, enhancing the efficiency of Ilex paraguariensis mass propagation. Full article
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