Bloodstream Infections

A special issue of Pathogens (ISSN 2076-0817).

Deadline for manuscript submissions: closed (15 December 2022) | Viewed by 6760

Special Issue Editor


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Guest Editor
Clinical Medicine Research Center, National Cheng Kung University Hospital, No. 138, Sheng Li Road, Tainan 70403, Taiwan
Interests: bloodstream infections; antimicrobial stewardship program; empirical antimicrobial therapy; sepsis
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Special Issue Information

Dear Colleagues,

Bloodstream infection (BSI) is a major cause of infectious disease morbidity and mortality worldwide. While a positive blood culture is mandatory for establishment of the presence of BSI, there are numerous determinants that must be considered for clinicians. Hence, there is a pressing need to study and develop new diagnoses and therapeutic strategies (such as the antimicrobial stewardship program) for BSIs. The aim of the current Special Issue is to collect, update, and harmonize the most recent literature on BSIs. We aim to touch herein the updated epidemiologic aspects of BSIs as well as their diagnosis and treatments, spanning from the understanding of the molecular mechanisms involved in pathogenesis, to the recapitulation of the host immune responses, to the analysis of available biomolecular tools for early diagnoses, and to the latest advancements in antimicrobial discovery.

As the Guest Editor of a Special Issue of Pathogens focused on BSIs, I invite you, as an expert in this field, to submit a manuscript for publication, in terms of original research, review articles, or short communications that report on recent progress to further expand host–pathogen knowledge.

Dr. Ching-Chi Lee
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Pathogens is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • bloodstream infections
  • bacteremia
  • empirical antibiotic
  • definitive antibiotic
  • antimicrobial stewardship program
  • early diagnosis
  • community-acquired
  • hospital-acquired

Published Papers (1 paper)

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Research

12 pages, 1033 KiB  
Article
Development of a Simple DNA Extraction Method and Candida Pan Loop-Mediated Isothermal Amplification Assay for Diagnosis of Candidemia
by Da Hye Lim, Hyunseul Jee, Kyung Chul Moon, Chae Seung Lim and Woong Sik Jang
Pathogens 2022, 11(2), 111; https://doi.org/10.3390/pathogens11020111 - 18 Jan 2022
Cited by 8 | Viewed by 6232
Abstract
To reduce the morbidity and mortality of candidemia patients through rapid treatment, the development of a simple, rapid molecular diagnostic method that is based on nucleic acid extraction and is superior to conventional methods for detecting Candida in the blood is necessary. We [...] Read more.
To reduce the morbidity and mortality of candidemia patients through rapid treatment, the development of a simple, rapid molecular diagnostic method that is based on nucleic acid extraction and is superior to conventional methods for detecting Candida in the blood is necessary. We developed a multiplex Candida Pan/internal control (IC) loop-mediated isothermal amplification (LAMP) assay and a simple DNA extraction boiling protocol using Chelex-100 that could extract yeast DNA in blood within 20 min. The Chelex-100/boiling method for DNA extraction showed comparable efficiency to that of the commercial QIAamp UCP Pathogen Mini Kit using Candida albicans qPCR. In addition, the Candida Pan/IC LAMP assay showed superior sensitivity to that of general Candida Pan and species qPCRs against clinical DNA samples extracted with the QIAamp UCP Pathogen Mini Kit and Chelex-100/boiling method. The Candida Pan/IC LAMP assay followed by Chelex-100/boiling-mediated DNA extraction showed high sensitivity (100%) and specificity (100%) against clinical samples infected with Candida. These results suggest that the Candida Pan/IC LAMP assay could be used as a rapid molecular diagnostic test for candidemia. Full article
(This article belongs to the Special Issue Bloodstream Infections)
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